ES2189888T3 - Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos. - Google Patents

Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos.

Info

Publication number
ES2189888T3
ES2189888T3 ES96937302T ES96937302T ES2189888T3 ES 2189888 T3 ES2189888 T3 ES 2189888T3 ES 96937302 T ES96937302 T ES 96937302T ES 96937302 T ES96937302 T ES 96937302T ES 2189888 T3 ES2189888 T3 ES 2189888T3
Authority
ES
Spain
Prior art keywords
dehydrogenase
endogenous
sample
analyte
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96937302T
Other languages
English (en)
Inventor
Anthony Joseph Delizza
Shireen Syrah Hussain
Jill Mccornack Visor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Application granted granted Critical
Publication of ES2189888T3 publication Critical patent/ES2189888T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION ES UNA MEJORA EN UN METODO QUE UTILIZA UNA DESHIDROGENASA MARCADORA PARA DETECTAR LA PRESENCIA DE UN ANALITO EN UNA MUESTRA EN LA QUE LAS DESHIDROGENASAS ENDOGENAS PRESENTES EN LA MUESTRA INTERFIEREN EN LA DETECCION DEL ANALITO. LA MEJORA CONSISTE EN LA INCLUSION DE UN INHIBIDOR DE LA DESHIDROGENASA ENDOGENA EN EL MEDIO DE ENSAYO. LA MEJORA ES PARTICULARMENTE UTIL ES LA MEDIDA DE ALCOHOL ETILICO UTILIZANDO ALCOHOL DESHIDROGENASA COMO ENZIMA MARCADOR CUANDO EL LACTATO Y LA LACTATO DESHIDROGENASA PRESENTES EN LA MUESTRA INTERFIEREN EN LA DETECCION DEL ANALITO. INHIBIDORES PARTICULARMENTE EFECTIVOS QUE EVITAN ESTA INTERFERENCIA SON LO ACIDOS GRASOS, TALES COMO EL ACIDO AGARICO Y LAURICO, Y SALES DE SULFATO DE ALQUILO, TALES COMO EL DODECIL SULFATO SODICO Y EL DECIL SULFATO SODICO.
ES96937302T 1995-11-02 1996-10-30 Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos. Expired - Lifetime ES2189888T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US613495P 1995-11-02 1995-11-02

Publications (1)

Publication Number Publication Date
ES2189888T3 true ES2189888T3 (es) 2003-07-16

Family

ID=21719474

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96937302T Expired - Lifetime ES2189888T3 (es) 1995-11-02 1996-10-30 Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos.

Country Status (6)

Country Link
EP (1) EP0865501B1 (es)
JP (1) JP3658691B2 (es)
CA (1) CA2236307A1 (es)
DE (1) DE69625617T2 (es)
ES (1) ES2189888T3 (es)
WO (1) WO1997016562A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861269A (en) * 1996-11-01 1999-01-19 Dade Behring Marburg Gmbh Methods for removing interferences due to endogenous dehydrogenases in enzyme assays

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO158197C (no) * 1980-04-09 1988-07-27 Nyegaard & Co As Reagens for bestemmelse av hydroksysteroider i serum.
DE3303098A1 (de) * 1983-01-31 1984-08-02 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur glucosebestimmung im haemolysierten blut
JPS6091998A (ja) * 1983-10-27 1985-05-23 Yukio Shigeta 体液及び尿中のｄ―３―ヒドロキシ酪酸の測定法並びにそのための測定試薬
US4937047A (en) * 1986-04-01 1990-06-26 Konishiroku Photo Industry Co., Ltd. Analytical element
IE920779A1 (en) * 1991-03-13 1992-09-23 Du Pont Selective stabilization of lactate dehydrogenase isoenzyme¹ld1 by high molecular weight polyols

Also Published As

Publication number Publication date
DE69625617D1 (de) 2003-02-06
JP3658691B2 (ja) 2005-06-08
EP0865501A1 (en) 1998-09-23
CA2236307A1 (en) 1997-05-09
EP0865501B1 (en) 2003-01-02
DE69625617T2 (de) 2003-08-21
WO1997016562A1 (en) 1997-05-09
JP2001507206A (ja) 2001-06-05

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