ES2190802T3 - Procedimientos y composiciones para la identificacion sensible, rapida y funcional de polinucleotidos genomicos, y su utilizacion para ensayos celulares en la puesta a punto de farmacos. - Google Patents

Procedimientos y composiciones para la identificacion sensible, rapida y funcional de polinucleotidos genomicos, y su utilizacion para ensayos celulares en la puesta a punto de farmacos.

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Publication number
ES2190802T3
ES2190802T3 ES97943625T ES97943625T ES2190802T3 ES 2190802 T3 ES2190802 T3 ES 2190802T3 ES 97943625 T ES97943625 T ES 97943625T ES 97943625 T ES97943625 T ES 97943625T ES 2190802 T3 ES2190802 T3 ES 2190802T3
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cell
pharmacos
procedures
sensitive
compositions
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ES97943625T
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Michael A Whitney
Paul A Negulescu
Frank Craig
Lora Mere
Gorden J Foulkes
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Vertex Pharmaceuticals San Diego LLC
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Vertex Pharmaceuticals San Diego LLC
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/5047—Cells of the immune system
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/986—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides (3.5.2), e.g. beta-lactamase (penicillinase, 3.5.2.6), creatinine amidohydrolase (creatininase, EC 3.5.2.10), N-methylhydantoinase (3.5.2.6)
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Abstract

LA PRESENTE INVENCION SE REFIERE A PROCEDIMIENTOS Y COMPOSICIONES PARA LA IDENTIFICACION DE PROTEINAS O SUSTANCIAS QUIMICAS QUE MODULAN DIRECTA E INDIRECTAMENTE UN POLINUCLEOTIDO GENOMICO, ASI COMO A PROCEDIMIENTOS DE IDENTIFICACION DE POLINUCLEOTIDOS GENOMICOS ACTIVOS. EN GENERAL, EL PROCEDIMIENTO CONSISTE EN INSERTAR UNA CONSTRUCCION DE EXPRESION DE BL (BETA LACTAMASA) EN UN GENOMA EUCARIOTICO, NORMALMENTE SIN LEVADURA, QUE CONTIENE, POR LO MENOS, UNA CELULA VIVA, QUE ENTRA EN CONTACTO CON LA CELULA QUE TIENE UNA CONCENTRACION PREDETERMINADA DE UN MODULADOR Y DETECTA LA ACTIVIDAD DE LA BL EN LA CELULA.
ES97943625T 1996-09-26 1997-09-26 Procedimientos y composiciones para la identificacion sensible, rapida y funcional de polinucleotidos genomicos, y su utilizacion para ensayos celulares en la puesta a punto de farmacos. Expired - Lifetime ES2190802T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/719,697 US5928888A (en) 1996-09-26 1996-09-26 Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities

Publications (1)

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ES2190802T3 true ES2190802T3 (es) 2003-08-16

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ES97943625T Expired - Lifetime ES2190802T3 (es) 1996-09-26 1997-09-26 Procedimientos y composiciones para la identificacion sensible, rapida y funcional de polinucleotidos genomicos, y su utilizacion para ensayos celulares en la puesta a punto de farmacos.
ES02010958T Expired - Lifetime ES2315327T4 (es) 1996-09-26 1997-09-26 Procedimientos y composiciones para determinar los perfiles de respuesta celular.

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ES02010958T Expired - Lifetime ES2315327T4 (es) 1996-09-26 1997-09-26 Procedimientos y composiciones para determinar los perfiles de respuesta celular.

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US (1) US5928888A (es)
EP (2) EP0952976B1 (es)
AT (2) ATE229508T1 (es)
AU (1) AU4505797A (es)
CA (2) CA2409901A1 (es)
DE (2) DE69739110D1 (es)
DK (2) DK1262478T3 (es)
ES (2) ES2190802T3 (es)
PT (1) PT1262478E (es)
WO (1) WO1998013353A1 (es)

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DK0952976T3 (da) 2003-04-07
ES2315327T3 (es) 2009-04-01
EP1262478B9 (en) 2009-04-15
EP1262478A2 (en) 2002-12-04
DE69739110D1 (de) 2008-12-24
US5928888A (en) 1999-07-27
PT1262478E (pt) 2009-01-20
EP1262478B1 (en) 2008-11-12
WO1998013353A1 (en) 1998-04-02
AU4505797A (en) 1998-04-17
ATE414165T1 (de) 2008-11-15
DE69717856T2 (de) 2004-02-19
ATE229508T1 (de) 2002-12-15
EP0952976A4 (es) 1999-12-01
EP1262478A3 (en) 2003-03-26
EP0952976A1 (en) 1999-11-03
CA2409901A1 (en) 1998-04-02
CA2266978C (en) 2005-06-07
EP0952976B1 (en) 2002-12-11
CA2266978A1 (en) 1998-04-02
ES2315327T4 (es) 2009-10-21
DK1262478T3 (da) 2009-02-09
DE69717856D1 (de) 2003-01-23

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