ES2192960B1 - Nuevo procedimiento de obtencion de acido hialuronico. - Google Patents
Nuevo procedimiento de obtencion de acido hialuronico.Info
- Publication number
- ES2192960B1 ES2192960B1 ES200102542A ES200102542A ES2192960B1 ES 2192960 B1 ES2192960 B1 ES 2192960B1 ES 200102542 A ES200102542 A ES 200102542A ES 200102542 A ES200102542 A ES 200102542A ES 2192960 B1 ES2192960 B1 ES 2192960B1
- Authority
- ES
- Spain
- Prior art keywords
- obtaining hyaluronic
- new procedure
- hyaluronic acid
- hydroalcoholic
- recovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 229920002674 hyaluronan Polymers 0.000 abstract 2
- 229960003160 hyaluronic acid Drugs 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 238000011084 recovery Methods 0.000 abstract 2
- 239000013049 sediment Substances 0.000 abstract 2
- 241000251468 Actinopterygii Species 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 238000011026 diafiltration Methods 0.000 abstract 1
- 238000004070 electrodeposition Methods 0.000 abstract 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 abstract 1
- 235000019799 monosodium phosphate Nutrition 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 abstract 1
- 239000001488 sodium phosphate Substances 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- 210000004127 vitreous body Anatomy 0.000 abstract 1
Landscapes
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
Abstract
Nuevo procedimiento de obtención de ácido hialurónico. El procedimiento de obtención de ácido hialurónico (AH) a partir del humor vítreo de peces, entre otros materiales biológicos de partida, descrita en esta invención se basa en la sucesión de: 1) una etapa de electrodeposición proteica, previa o simultánea a un proceso de diafiltración con recirculación total, 2) una recuperación selectiva en solución hidroalcohólica de sedimentos impuros obtenidos por precipitación alcohólica exhaustiva, 3) un tratamiento alcalino en solución hidroalcohólica y condiciones controladas de alcalinidad, temperatura, proporción de etanol y tiempo, que precipita el AH y solubiliza proteínas, y 4) la recuperación del AH por resuspensión del precipitado alcalino en fosfato monosódico hidroalcohólico, que disuelve el AH, neutraliza el extracto y deja en el sedimento los restos proteicos insolubles. Se obtienen de este modo preparados de AH de alta pureza (superior al 99,5 %), aptos para aplicaciones en clínica ycosmética.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200102542A ES2192960B1 (es) | 2001-11-16 | 2001-11-16 | Nuevo procedimiento de obtencion de acido hialuronico. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200102542A ES2192960B1 (es) | 2001-11-16 | 2001-11-16 | Nuevo procedimiento de obtencion de acido hialuronico. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2192960A1 ES2192960A1 (es) | 2003-10-16 |
| ES2192960B1 true ES2192960B1 (es) | 2005-03-01 |
Family
ID=29724693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200102542A Expired - Fee Related ES2192960B1 (es) | 2001-11-16 | 2001-11-16 | Nuevo procedimiento de obtencion de acido hialuronico. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2192960B1 (es) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4879375A (en) * | 1985-05-09 | 1989-11-07 | Hill David Cullis | Preparation of hyaluronic acid from synovial fluid |
| RU2074196C1 (ru) * | 1993-08-23 | 1997-02-27 | Научно-производственная фирма "Нарт" | Способ получения гиалуроновой кислоты |
| WO2000044925A1 (en) * | 1999-01-28 | 2000-08-03 | Chemedica S.A. | Process for purifying high molecular weight hyaluronic acid |
| RU2157381C1 (ru) * | 1999-03-01 | 2000-10-10 | Общество с ограниченной ответственностью "Научно-производственное предприятие "Тульская индустрия ЛТД" | Способ получения гиалуроновой кислоты |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59166504A (ja) * | 1983-03-10 | 1984-09-19 | Ichimaru Fuarukosu Kk | ヒアルロン酸の製造法 |
| JP2893451B2 (ja) * | 1988-08-09 | 1999-05-24 | 昭和産業株式会社 | 高分子量のヒアルロン酸を製造する方法 |
-
2001
- 2001-11-16 ES ES200102542A patent/ES2192960B1/es not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4879375A (en) * | 1985-05-09 | 1989-11-07 | Hill David Cullis | Preparation of hyaluronic acid from synovial fluid |
| RU2074196C1 (ru) * | 1993-08-23 | 1997-02-27 | Научно-производственная фирма "Нарт" | Способ получения гиалуроновой кислоты |
| WO2000044925A1 (en) * | 1999-01-28 | 2000-08-03 | Chemedica S.A. | Process for purifying high molecular weight hyaluronic acid |
| RU2157381C1 (ru) * | 1999-03-01 | 2000-10-10 | Общество с ограниченной ответственностью "Научно-производственное предприятие "Тульская индустрия ЛТД" | Способ получения гиалуроновой кислоты |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2192960A1 (es) | 2003-10-16 |
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