ES2198216B1 - Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones. - Google Patents

Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.

Info

Publication number
ES2198216B1
ES2198216B1 ES200201540A ES200201540A ES2198216B1 ES 2198216 B1 ES2198216 B1 ES 2198216B1 ES 200201540 A ES200201540 A ES 200201540A ES 200201540 A ES200201540 A ES 200201540A ES 2198216 B1 ES2198216 B1 ES 2198216B1
Authority
ES
Spain
Prior art keywords
autologous
applications
culture medium
human
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200201540A
Other languages
English (en)
Other versions
ES2198216A1 (es
Inventor
Felipe Prosper Cardoso
Jesus Herreros Gonzalez
Juan Carlos Chacques
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Navarra
Original Assignee
CHACQUES (33%)
Instituto Cientifico y Tecnologico de Navarra SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES200201540A priority Critical patent/ES2198216B1/es
Application filed by CHACQUES (33%), Instituto Cientifico y Tecnologico de Navarra SA filed Critical CHACQUES (33%)
Priority to KR1020087023967A priority patent/KR20080091874A/ko
Priority to BR0312365-0A priority patent/BR0312365A/pt
Priority to PCT/ES2003/000285 priority patent/WO2004005494A1/es
Priority to AU2003240857A priority patent/AU2003240857B2/en
Priority to US10/519,974 priority patent/US20060110368A1/en
Priority to RU2005102061/13A priority patent/RU2312141C2/ru
Priority to MXPA04012601A priority patent/MXPA04012601A/es
Priority to NZ537397A priority patent/NZ537397A/en
Priority to JP2004518793A priority patent/JP5379340B2/ja
Priority to KR1020057000034A priority patent/KR100960173B1/ko
Priority to EP03730223A priority patent/EP1972684A1/en
Priority to CNB038155990A priority patent/CN100572527C/zh
Publication of ES2198216A1 publication Critical patent/ES2198216A1/es
Priority to IL16596904A priority patent/IL165969A0/xx
Application granted granted Critical
Publication of ES2198216B1 publication Critical patent/ES2198216B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C12N5/0659—Satellite cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Medio de cultivo de células madre-progenitoras autólogas humanas y sus aplicaciones. El medio de cultivo autólogo de células madre-progenitoras autólogas humanas comprende: entre 0,1% y 90% en peso de suero humano autólogo; entre 0,1 y 10.000 Ul/ml de heparina; entre 0,1 y 10.000 Ul/ml de protamina; y un medio de cultivo con nutrientes básicos con o sin glutamina, en cantidad suficiente hasta el 100% en peso, y es útil para cultivar y expandir células madre-progenitoras autólogas humanas. Composiciones conteniendo dichas células pueden ser implantadas en el paciente mediante un procedimiento de cardiomioplastia celular autóloga para crear, regenerar y reparar tejido miocárdico disfuncional.
ES200201540A 2002-07-02 2002-07-02 Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones. Expired - Fee Related ES2198216B1 (es)

Priority Applications (14)

Application Number Priority Date Filing Date Title
ES200201540A ES2198216B1 (es) 2002-07-02 2002-07-02 Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.
KR1020057000034A KR100960173B1 (ko) 2002-07-02 2003-06-11 인간 원종 줄기세포들을 자가 배양하기 위한 배지와 그의응용
PCT/ES2003/000285 WO2004005494A1 (es) 2002-07-02 2003-06-11 Medio de cultivo de células madre-progenitoras autólogas humanas y sus aplicaciones
AU2003240857A AU2003240857B2 (en) 2002-07-02 2003-06-11 Medium for culturing autologous human progenitor stem cells and applications thereof
US10/519,974 US20060110368A1 (en) 2002-07-02 2003-06-11 Medium for culturing autologous human progenitor stem cells and applications thereof
RU2005102061/13A RU2312141C2 (ru) 2002-07-02 2003-06-11 Среда для культивирования аутологических человеческих стволовых клеток-предшественников и способы их применения
MXPA04012601A MXPA04012601A (es) 2002-07-02 2003-06-11 Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.
NZ537397A NZ537397A (en) 2002-07-02 2003-06-11 Use of an autologous culture medium comprising autologous human serum and nutrients, together with an anticoagulant, allows the in vitro culture, purification and expansion of autologous human progenitor stem cells
KR1020087023967A KR20080091874A (ko) 2002-07-02 2003-06-11 인간 원종 줄기세포들을 자가 배양하기 위한 배지와 그의 응용
BR0312365-0A BR0312365A (pt) 2002-07-02 2003-06-11 Meio de cultura autólogo de células-mães progenitoras autólogas humanas, métodos para a preparação do mesmo e de uma composição de células-mães progenitoras autólogas humanas e para a obtenção de células-mães progenitoras musculares autólogas humanas, emprego de um meio de cultura autólogo de células-mães progenitoras autólogas humanas, procedimento para a obtenção de células-mães progenitoras musculares autólogas humanas e procedimento terapêutico de cardiomioplastia celular autóloga, composição enriquecida com células-mães progenitoras musculares autólogas humanas e composição farmacêutica
EP03730223A EP1972684A1 (en) 2002-07-02 2003-06-11 Medium for culturing autologous human progenitor stem cells and applications
CNB038155990A CN100572527C (zh) 2002-07-02 2003-06-11 人体原始干细胞自体同源培养基及其应用
JP2004518793A JP5379340B2 (ja) 2002-07-02 2003-06-11 ヒト自己前駆幹細胞を培養する培地とその応用
IL16596904A IL165969A0 (en) 2002-07-02 2004-12-23 Medium for culturing aurologous human progenitor stem cells and applications thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200201540A ES2198216B1 (es) 2002-07-02 2002-07-02 Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.

Publications (2)

Publication Number Publication Date
ES2198216A1 ES2198216A1 (es) 2004-01-16
ES2198216B1 true ES2198216B1 (es) 2005-04-16

Family

ID=30011351

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200201540A Expired - Fee Related ES2198216B1 (es) 2002-07-02 2002-07-02 Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.

Country Status (13)

Country Link
US (1) US20060110368A1 (es)
EP (1) EP1972684A1 (es)
JP (1) JP5379340B2 (es)
KR (2) KR20080091874A (es)
CN (1) CN100572527C (es)
AU (1) AU2003240857B2 (es)
BR (1) BR0312365A (es)
ES (1) ES2198216B1 (es)
IL (1) IL165969A0 (es)
MX (1) MXPA04012601A (es)
NZ (1) NZ537397A (es)
RU (1) RU2312141C2 (es)
WO (1) WO2004005494A1 (es)

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US7253735B2 (en) 2003-03-24 2007-08-07 Alien Technology Corporation RFID tags and processes for producing RFID tags
DE102004025684B4 (de) 2004-04-29 2024-08-22 OSRAM Opto Semiconductors Gesellschaft mit beschränkter Haftung Verfahren zum Ausbilden einer Kontaktstruktur zur elektrischen Kontaktierung eines optoelektronischen Halbleiterchips
US8048999B2 (en) * 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
AU2008298816B2 (en) 2007-09-11 2013-09-26 Sapporo Medical University Cell growth method and pharmaceutical preparation for tissue repair and regeneration
RU2355424C1 (ru) * 2008-01-09 2009-05-20 Государственное образовательное учреждение дополнительного профессионального образования "Новокузнецкий государственный институт усовершенствования врачей Федерального агентства по здравоохранению и социальному развитию" Способ получения биоматрицы в эксперименте
WO2009119073A1 (ja) * 2008-03-28 2009-10-01 北海道公立大学法人札幌医科大学 皮膚の老化や瘢痕の治療剤
RU2493250C2 (ru) * 2011-07-08 2013-09-20 Общество с ограниченной ответственностью Научно-производственное предприятие "ПанЭко" Малосывороточная среда для культивирования фибробластов человека
JP5598864B2 (ja) * 2011-12-05 2014-10-01 北海道公立大学法人 札幌医科大学 細胞増殖方法ならびに組織の修復および再生のための医薬
RU2506309C2 (ru) * 2012-03-11 2014-02-10 Екатерина Алексеевна Федорова Способ культивирования миобластов in vitro для получения биомассы миоцитов для пищевых целей
TWI643953B (zh) * 2014-12-11 2018-12-11 訊聯生物科技股份有限公司 以自體血漿培養原始細胞的方法
CN106110304A (zh) * 2016-07-28 2016-11-16 广州赛莱拉干细胞科技股份有限公司 一种祛疤组合物和敷料
KR102093810B1 (ko) * 2017-09-19 2020-03-26 서울대학교산학협력단 면역원성 감소 방법
KR102118522B1 (ko) 2017-10-25 2020-06-03 (주)셀라토즈테라퓨틱스 신규한 근골격계 줄기세포
AU2020261541B2 (en) * 2019-04-23 2024-03-14 Cellatoz Therapeutics, Inc. Method for regulation of selective differentiation of musculoskeletal stem cells
EP3979997A1 (en) * 2019-06-06 2022-04-13 Novo Nordisk A/S Application of antiarrhythmic agents to stem cell derived cardiomyocytes and uses thereof
CN112501035B (zh) * 2020-12-09 2022-09-20 云南大学 基于捕食线虫真菌细胞膜破裂和内体增多而产生捕食器官的方法

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Also Published As

Publication number Publication date
BR0312365A (pt) 2005-04-05
ES2198216A1 (es) 2004-01-16
JP5379340B2 (ja) 2013-12-25
US20060110368A1 (en) 2006-05-25
EP1972684A1 (en) 2008-09-24
MXPA04012601A (es) 2005-09-30
KR20050037549A (ko) 2005-04-22
AU2003240857B2 (en) 2008-06-26
RU2312141C2 (ru) 2007-12-10
KR20080091874A (ko) 2008-10-14
RU2005102061A (ru) 2005-10-10
CN1665922A (zh) 2005-09-07
AU2003240857A1 (en) 2004-01-23
WO2004005494A1 (es) 2004-01-15
KR100960173B1 (ko) 2010-05-26
IL165969A0 (en) 2006-01-15
CN100572527C (zh) 2009-12-23
NZ537397A (en) 2008-09-26
JP2005531322A (ja) 2005-10-20

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