ES2218535T3 - Metodo para el enriquecimiento de celulas modificadas mediante. - Google Patents

Metodo para el enriquecimiento de celulas modificadas mediante.

Info

Publication number
ES2218535T3
ES2218535T3 ES95250109T ES95250109T ES2218535T3 ES 2218535 T3 ES2218535 T3 ES 2218535T3 ES 95250109 T ES95250109 T ES 95250109T ES 95250109 T ES95250109 T ES 95250109T ES 2218535 T3 ES2218535 T3 ES 2218535T3
Authority
ES
Spain
Prior art keywords
cells
magnetic
microprojectiles
sequences
magnetic field
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95250109T
Other languages
English (en)
Spanish (es)
Inventor
Burghardt Prof. Dr. Wittig
Matthias Schroff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mologen AG
Original Assignee
Mologen AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mologen AG filed Critical Mologen AG
Application granted granted Critical
Publication of ES2218535T3 publication Critical patent/ES2218535T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ES95250109T 1994-05-09 1995-05-08 Metodo para el enriquecimiento de celulas modificadas mediante. Expired - Lifetime ES2218535T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4416784A DE4416784A1 (de) 1994-05-09 1994-05-09 Methode zur Anreicherung von Zellen, die durch ballistischen Transfer modifiziert wurden
DE4416784 1994-05-09

Publications (1)

Publication Number Publication Date
ES2218535T3 true ES2218535T3 (es) 2004-11-16

Family

ID=6517973

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95250109T Expired - Lifetime ES2218535T3 (es) 1994-05-09 1995-05-08 Metodo para el enriquecimiento de celulas modificadas mediante.

Country Status (5)

Country Link
US (1) US6348338B1 (de)
EP (1) EP0686697B1 (de)
AT (1) ATE264399T1 (de)
DE (2) DE4416784A1 (de)
ES (1) ES2218535T3 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19612001A1 (de) * 1996-03-18 1997-09-25 Silica Gel Gmbh Adsorptions Te Superparamagnetische Teilchen mit vergrößerter R¶1¶-Relaxivität, Verfahren zur Herstellung und deren Verwendung
DE19648625A1 (de) * 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
DE19648656B4 (de) * 1996-11-14 2005-08-11 Hiper Ceramics Gmbh Vorrichtung zum Beschleunigen von Partikeln auf Zellen zum ballistischen Transfer
US6284345B1 (en) 1997-12-08 2001-09-04 Washington University Designer particles of micron and submicron dimension
PL1699480T3 (pl) 2003-12-30 2011-11-30 Mologen Ag Allogeniczny przeciwnowotworowy czynnik terapeutyczny
US20070016985A1 (en) * 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Particle Preparation for Direct-Delivery Transformation
ES2390132T3 (es) 2005-07-18 2012-11-06 Pioneer Hi-Bred International Inc. Sitios de recombinación FRT modificados y métodos de uso
US11484724B2 (en) 2015-09-30 2022-11-01 Btl Medical Solutions A.S. Methods and devices for tissue treatment using mechanical stimulation and electromagnetic field
US12220380B2 (en) 2015-09-30 2025-02-11 Btl Medical Solutions A.S. Methods and devices for tissue treatment using mechanical stimulation and electromagnetic field

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) * 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
AU4746590A (en) * 1988-12-28 1990-08-01 Stefan Miltenyi Methods and materials for high gradient magnetic separation of biological materials
ES2113371T3 (es) * 1989-11-16 1998-05-01 Univ Duke Transformacion de celulas epidermicas de tejidos animales con la ayuda de particulas.
US5066587A (en) * 1990-01-26 1991-11-19 The Upjohn Company Gas driven microprojectile accelerator and method of use
NZ239977A (en) * 1990-11-14 1993-08-26 Pioneer Hi Bred Int Transforming plants by the use of agrobacterium
EP0706576A1 (de) * 1993-06-30 1996-04-17 Dcv Biologics L.P. Eine methode zur ineinfühlung von einem biologische stoff in ein ziel

Also Published As

Publication number Publication date
EP0686697A3 (de) 1997-03-12
US6348338B1 (en) 2002-02-19
DE4416784A1 (de) 1995-11-30
EP0686697A2 (de) 1995-12-13
EP0686697B1 (de) 2004-04-14
DE59510890D1 (de) 2004-05-19
ATE264399T1 (de) 2004-04-15

Similar Documents

Publication Publication Date Title
Olden et al. Cationic polymers for non-viral gene delivery to human T cells
ES2218535T3 (es) Metodo para el enriquecimiento de celulas modificadas mediante.
JP2006517790A (ja) ポリペプチド−核酸複合体の細胞の送達および活性化
Liang et al. A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy
RU2014129632A (ru) Комбинированная терапия для стабильного и долговременного приживления трансплантата с использованием конкретных протоколов для т/в-клеточной деплеции
WO2012138858A1 (en) Reversing the effects of the tumor microenvironment using chimeric cytokine receptors
Li et al. Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner
CN105315377A (zh) 带有穿膜肽的tcs融合蛋白、质粒及制备方法与应用
CN110713977B (zh) 一种cd8 t细胞的培养扩增方法
US10294199B2 (en) Propyl cationic peptide lipids, synthesis method thereof, and application thereof
CN105143441A (zh) 用带有活性因子的细胞空壳作为淋巴细胞体外培养增效剂的制备及其应用方法
US20090004742A1 (en) Selection of antigen-specific t cells
IL189559A (en) Nanoparticle suitable for delivery of a biomolecule into or out of a membrane enclosed cell or cell organelle
Zhang et al. Polyethyleneimine-coated Fe3O4 nanoparticles for efficient siRNA delivery to human mesenchymal stem cells derived from different tissues
ES2319942T3 (es) Procedimientos para la produccion de celulas t tcr gamma delta.
ES2336302T3 (es) Produccion de celulas ttcr gamma delta.
CN116656619A (zh) 用于胰腺癌治疗的工程化外泌体
CN114377122B (zh) 一种基于四面体框架核酸的复合佐剂、mRNA疫苗及其制备方法和用途
ES2218221T3 (es) Metodo de poracion de membranas biologicas.
Shimasaki et al. Engineering of natural killer cells for clinical application
EP3004153B1 (de) Rekombinantes cytokin für eine krebstherapie
CN115678847A (zh) 一种体外扩增及活化γδ-T细胞的方法
CN113583953B (zh) 制备过表达外源基因的细胞的方法
CN116785416A (zh) 一种树突状细胞外泌体肝癌疫苗制备方法
JP7337373B2 (ja) 抗原特異的ｔ細胞の製造方法