ES2252005T3 - Transformacion de allium sp. con agrobacterium utilizando cultivos de callos embriogenicos. - Google Patents

Transformacion de allium sp. con agrobacterium utilizando cultivos de callos embriogenicos.

Info

Publication number
ES2252005T3
ES2252005T3 ES00932149T ES00932149T ES2252005T3 ES 2252005 T3 ES2252005 T3 ES 2252005T3 ES 00932149 T ES00932149 T ES 00932149T ES 00932149 T ES00932149 T ES 00932149T ES 2252005 T3 ES2252005 T3 ES 2252005T3
Authority
ES
Spain
Prior art keywords
allium
agrobacterium
embriogenic
streets
crops
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES00932149T
Other languages
English (en)
Inventor
John Reynolds
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seminis Vegetable Seeds Inc
Original Assignee
Seminis Vegetable Seeds Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seminis Vegetable Seeds Inc filed Critical Seminis Vegetable Seeds Inc
Application granted granted Critical
Publication of ES2252005T3 publication Critical patent/ES2252005T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para transformar una especie de Allium con un gen heterólogo, el método comprendiendo la etapa de: poner en contacto el material embriogénico de callo de una especie Allium con una bacteria que pertenece al género Agrobacterium que contiene un gen heterólogo.
ES00932149T 1999-05-05 2000-05-05 Transformacion de allium sp. con agrobacterium utilizando cultivos de callos embriogenicos. Expired - Lifetime ES2252005T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13261799P 1999-05-05 1999-05-05

Publications (1)

Publication Number Publication Date
ES2252005T3 true ES2252005T3 (es) 2006-05-16

Family

ID=22454849

Family Applications (1)

Application Number Title Priority Date Filing Date
ES00932149T Expired - Lifetime ES2252005T3 (es) 1999-05-05 2000-05-05 Transformacion de allium sp. con agrobacterium utilizando cultivos de callos embriogenicos.

Country Status (4)

Country Link
EP (1) EP1180927B1 (es)
AU (1) AU780954B2 (es)
ES (1) ES2252005T3 (es)
WO (1) WO2000065903A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU763531C (en) * 1999-01-29 2004-02-26 The New Zealand Institute For Plant And Food Research Limited Transformation and regeneration of allium plants
NZ562781A (en) * 2007-10-24 2010-02-26 Nz Inst Plant & Food Res Ltd Allium transformation
CN111543320B (zh) * 2020-05-11 2021-09-21 天津师范大学 一种利用大蒜愈伤组织生产大蒜素的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593874A (en) * 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
JPH0884540A (ja) * 1994-07-18 1996-04-02 Sumitomo Chem Co Ltd 植物における病害虫抵抗性増強方法
EP0900279B1 (en) * 1997-02-20 2004-09-29 Bayer BioScience N.V. Improved transformation method for plants
WO1999010512A1 (en) * 1997-08-25 1999-03-04 Nunhems Zaden B.V. Improved agrobacterium-mediated transformation of plants
AU763531C (en) * 1999-01-29 2004-02-26 The New Zealand Institute For Plant And Food Research Limited Transformation and regeneration of allium plants
CA2267014A1 (en) * 1999-03-26 2000-09-26 Brenda Rojas Monocot transformation using acrobacterium

Also Published As

Publication number Publication date
AU780954B2 (en) 2005-04-28
EP1180927B1 (en) 2005-12-21
AU4991800A (en) 2000-11-17
EP1180927A1 (en) 2002-02-27
EP1180927A4 (en) 2002-06-19
WO2000065903A1 (en) 2000-11-09

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