ES2347574T3 - Compuestos de arn de doble hebra especificos de vglut. - Google Patents

Compuestos de arn de doble hebra especificos de vglut. Download PDF

Info

Publication number: ES2347574T3
Authority: ES; Spain
Prior art keywords: cells; sirna; dna; vglut2; cell
Prior art date: 2004-03-10
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES05707623T

Other languages

English (en)

Spanish (es)

Inventor

Clemens Gillen

Gregor Bahrenberg

Thomas Christoph

Eberhard Weihe

K.-H. Martin Schafer

Florian Bender

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Gruenenthal GmbH

Original Assignee

Gruenenthal GmbH

Chemie Gruenenthal GmbH

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2004-03-10

Filing date

2005-02-24

Publication date

2010-11-02

2005-02-24 Application filed by Gruenenthal GmbH, Chemie Gruenenthal GmbH filed Critical Gruenenthal GmbH

2010-11-02 Application granted granted Critical

2010-11-02 Publication of ES2347574T3 publication Critical patent/ES2347574T3/es

2025-02-24 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

102000016508 VGLUT Human genes 0.000 title claims abstract description 17
108060004582 VGLUT Proteins 0.000 title claims abstract description 17
150000001875 compounds Chemical class 0.000 title description 5
108020004459 Small interfering RNA Proteins 0.000 claims abstract description 68
210000004027 cell Anatomy 0.000 claims description 282
238000000034 method Methods 0.000 claims description 62
239000000126 substance Substances 0.000 claims description 56
241000282414 Homo sapiens Species 0.000 claims description 53
230000014509 gene expression Effects 0.000 claims description 52
208000002193 Pain Diseases 0.000 claims description 37
238000011282 treatment Methods 0.000 claims description 35
239000003814 drug Substances 0.000 claims description 22
238000001727 in vivo Methods 0.000 claims description 17
238000000338 in vitro Methods 0.000 claims description 15
239000002502 liposome Substances 0.000 claims description 10
208000000094 Chronic Pain Diseases 0.000 claims description 8
239000000654 additive Substances 0.000 claims description 8
238000004519 manufacturing process Methods 0.000 claims description 8
208000004454 Hyperalgesia Diseases 0.000 claims description 7
206010028980 Neoplasm Diseases 0.000 claims description 7
230000000202 analgesic effect Effects 0.000 claims description 6
229940079593 drug Drugs 0.000 claims description 6
208000024891 symptom Diseases 0.000 claims description 5
206010061218 Inflammation Diseases 0.000 claims description 4
210000000234 capsid Anatomy 0.000 claims description 4
230000004054 inflammatory process Effects 0.000 claims description 4
230000003612 virological effect Effects 0.000 claims description 4
208000003251 Pruritus Diseases 0.000 claims description 3
206010046543 Urinary incontinence Diseases 0.000 claims description 3
238000001415 gene therapy Methods 0.000 claims description 3
206010065390 Inflammatory pain Diseases 0.000 claims description 2
208000006673 asthma Diseases 0.000 claims description 2
210000001671 embryonic stem cell Anatomy 0.000 claims description 2
238000006911 enzymatic reaction Methods 0.000 claims description 2
230000001272 neurogenic effect Effects 0.000 claims description 2
230000002401 inhibitory effect Effects 0.000 claims 1
108091006162 SLC17A6 Proteins 0.000 description 159
102000046053 Vesicular Glutamate Transport Protein 2 Human genes 0.000 description 158
108020004414 DNA Proteins 0.000 description 156
238000001890 transfection Methods 0.000 description 79
239000002609 medium Substances 0.000 description 45
108020004999 messenger RNA Proteins 0.000 description 45
238000012360 testing method Methods 0.000 description 45
108091006283 SLC17A7 Proteins 0.000 description 41
102100038033 Vesicular glutamate transporter 3 Human genes 0.000 description 41
108091028043 Nucleic acid sequence Proteins 0.000 description 40
101150086693 Slc17a8 gene Proteins 0.000 description 40
102000001763 Vesicular Glutamate Transport Proteins Human genes 0.000 description 40
108010040170 Vesicular Glutamate Transport Proteins Proteins 0.000 description 40
125000003729 nucleotide group Chemical group 0.000 description 40
108090000623 proteins and genes Proteins 0.000 description 40
102100038039 Vesicular glutamate transporter 1 Human genes 0.000 description 39
239000002773 nucleotide Substances 0.000 description 39
210000002569 neuron Anatomy 0.000 description 38
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 38
239000000243 solution Substances 0.000 description 37
239000013612 plasmid Substances 0.000 description 30
102000004169 proteins and genes Human genes 0.000 description 29
239000000203 mixture Substances 0.000 description 28
235000018102 proteins Nutrition 0.000 description 26
210000003594 spinal ganglia Anatomy 0.000 description 26
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 24
238000003752 polymerase chain reaction Methods 0.000 description 24
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
238000002360 preparation method Methods 0.000 description 22
239000000872 buffer Substances 0.000 description 20
238000005119 centrifugation Methods 0.000 description 20
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 19
239000013598 vector Substances 0.000 description 18
239000008188 pellet Substances 0.000 description 17
241000283973 Oryctolagus cuniculus Species 0.000 description 16
230000000694 effects Effects 0.000 description 16
238000013518 transcription Methods 0.000 description 16
230000035897 transcription Effects 0.000 description 16
241001465754 Metazoa Species 0.000 description 15
241000700159 Rattus Species 0.000 description 15
230000015572 biosynthetic process Effects 0.000 description 15
239000002299 complementary DNA Substances 0.000 description 15
238000011534 incubation Methods 0.000 description 15
238000000746 purification Methods 0.000 description 15
230000002068 genetic effect Effects 0.000 description 14
102000039446 nucleic acids Human genes 0.000 description 14
108020004707 nucleic acids Proteins 0.000 description 14
150000007523 nucleic acids Chemical class 0.000 description 14
239000006228 supernatant Substances 0.000 description 14
210000001519 tissue Anatomy 0.000 description 14
238000012228 RNA interference-mediated gene silencing Methods 0.000 description 13
238000006243 chemical reaction Methods 0.000 description 13
230000000295 complement effect Effects 0.000 description 13
230000009368 gene silencing by RNA Effects 0.000 description 13
230000001629 suppression Effects 0.000 description 13
241000894006 Bacteria Species 0.000 description 12
108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 description 12
102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 description 12
102000004190 Enzymes Human genes 0.000 description 12
108090000790 Enzymes Proteins 0.000 description 12
QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
229940088598 enzyme Drugs 0.000 description 12
238000003786 synthesis reaction Methods 0.000 description 12
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 11
102000003566 TRPV1 Human genes 0.000 description 11
101150016206 Trpv1 gene Proteins 0.000 description 11
230000000692 anti-sense effect Effects 0.000 description 11
238000010494 dissociation reaction Methods 0.000 description 11
230000005593 dissociations Effects 0.000 description 11
210000003527 eukaryotic cell Anatomy 0.000 description 11
229930195712 glutamate Natural products 0.000 description 11
239000001963 growth medium Substances 0.000 description 11
230000005764 inhibitory process Effects 0.000 description 11
238000005259 measurement Methods 0.000 description 11
239000000047 product Substances 0.000 description 11
102100023387 Endoribonuclease Dicer Human genes 0.000 description 10
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
101000907904 Homo sapiens Endoribonuclease Dicer Proteins 0.000 description 10
102000004142 Trypsin Human genes 0.000 description 10
108090000631 Trypsin Proteins 0.000 description 10
238000010521 absorption reaction Methods 0.000 description 10
230000029087 digestion Effects 0.000 description 10
239000007758 minimum essential medium Substances 0.000 description 10
229920000729 poly(L-lysine) polymer Polymers 0.000 description 10
238000011160 research Methods 0.000 description 10
238000002560 therapeutic procedure Methods 0.000 description 10
239000012588 trypsin Substances 0.000 description 10
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
108091034117 Oligonucleotide Proteins 0.000 description 9
102100026260 Titin Human genes 0.000 description 9
239000003153 chemical reaction reagent Substances 0.000 description 9
239000011780 sodium chloride Substances 0.000 description 9
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
241000283074 Equus asinus Species 0.000 description 8
108010025020 Nerve Growth Factor Proteins 0.000 description 8
102000015336 Nerve Growth Factor Human genes 0.000 description 8
102100025038 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Human genes 0.000 description 8
101710186825 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Proteins 0.000 description 8
230000001580 bacterial effect Effects 0.000 description 8
230000003115 biocidal effect Effects 0.000 description 8
235000011089 carbon dioxide Nutrition 0.000 description 8
230000015556 catabolic process Effects 0.000 description 8
238000004113 cell culture Methods 0.000 description 8
238000006731 degradation reaction Methods 0.000 description 8
238000010790 dilution Methods 0.000 description 8
239000012895 dilution Substances 0.000 description 8
238000002474 experimental method Methods 0.000 description 8
238000010353 genetic engineering Methods 0.000 description 8
239000000463 material Substances 0.000 description 8
239000012528 membrane Substances 0.000 description 8
229940053128 nerve growth factor Drugs 0.000 description 8
230000009467 reduction Effects 0.000 description 8
239000000725 suspension Substances 0.000 description 8
230000009466 transformation Effects 0.000 description 8
FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 7
241000700199 Cavia porcellus Species 0.000 description 7
102100039289 Glial fibrillary acidic protein Human genes 0.000 description 7
101710193519 Glial fibrillary acidic protein Proteins 0.000 description 7
210000000170 cell membrane Anatomy 0.000 description 7
210000003855 cell nucleus Anatomy 0.000 description 7
239000006285 cell suspension Substances 0.000 description 7
210000005046 glial fibrillary acidic protein Anatomy 0.000 description 7
230000003993 interaction Effects 0.000 description 7
238000002372 labelling Methods 0.000 description 7
210000000929 nociceptor Anatomy 0.000 description 7
239000002243 precursor Substances 0.000 description 7
238000012545 processing Methods 0.000 description 7
108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
102000040650 (ribonucleotides)n+m Human genes 0.000 description 6
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 6
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
241000700157 Rattus norvegicus Species 0.000 description 6
101100428530 Rattus norvegicus Slc17a6 gene Proteins 0.000 description 6
108091027967 Small hairpin RNA Proteins 0.000 description 6
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
239000003242 anti bacterial agent Substances 0.000 description 6
229940088710 antibiotic agent Drugs 0.000 description 6
238000010195 expression analysis Methods 0.000 description 6
238000000799 fluorescence microscopy Methods 0.000 description 6
210000004379 membrane Anatomy 0.000 description 6
108091008700 nociceptors Proteins 0.000 description 6
230000008569 process Effects 0.000 description 6
239000012096 transfection reagent Substances 0.000 description 6
241000588724 Escherichia coli Species 0.000 description 5
230000004913 activation Effects 0.000 description 5
238000013019 agitation Methods 0.000 description 5
238000000540 analysis of variance Methods 0.000 description 5
238000003556 assay Methods 0.000 description 5
239000003086 colorant Substances 0.000 description 5
238000010276 construction Methods 0.000 description 5
238000010828 elution Methods 0.000 description 5
239000012737 fresh medium Substances 0.000 description 5
239000000499 gel Substances 0.000 description 5
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 5
239000010410 layer Substances 0.000 description 5
210000004962 mammalian cell Anatomy 0.000 description 5
230000004048 modification Effects 0.000 description 5
238000012986 modification Methods 0.000 description 5
230000001537 neural effect Effects 0.000 description 5
210000000278 spinal cord Anatomy 0.000 description 5
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
108010078791 Carrier Proteins Proteins 0.000 description 4
241000699800 Cricetinae Species 0.000 description 4
102000007260 Deoxyribonuclease I Human genes 0.000 description 4
108010008532 Deoxyribonuclease I Proteins 0.000 description 4
102000016911 Deoxyribonucleases Human genes 0.000 description 4
108010053770 Deoxyribonucleases Proteins 0.000 description 4
102100034349 Integrase Human genes 0.000 description 4
241001430197 Mollicutes Species 0.000 description 4
241000699666 Mus <mouse, genus> Species 0.000 description 4
108091081024 Start codon Proteins 0.000 description 4
239000007983 Tris buffer Substances 0.000 description 4
238000000246 agarose gel electrophoresis Methods 0.000 description 4
150000001413 amino acids Chemical group 0.000 description 4
229910052791 calcium Inorganic materials 0.000 description 4
239000011575 calcium Substances 0.000 description 4
230000001413 cellular effect Effects 0.000 description 4
230000000052 comparative effect Effects 0.000 description 4
230000006378 damage Effects 0.000 description 4
238000001514 detection method Methods 0.000 description 4
230000002255 enzymatic effect Effects 0.000 description 4
238000000605 extraction Methods 0.000 description 4
239000012634 fragment Substances 0.000 description 4
238000007710 freezing Methods 0.000 description 4
230000008014 freezing Effects 0.000 description 4
230000006870 function Effects 0.000 description 4
235000011187 glycerol Nutrition 0.000 description 4
238000009396 hybridization Methods 0.000 description 4
229910052739 hydrogen Inorganic materials 0.000 description 4
238000010166 immunofluorescence Methods 0.000 description 4
230000001965 increasing effect Effects 0.000 description 4
239000003112 inhibitor Substances 0.000 description 4
239000007788 liquid Substances 0.000 description 4
210000005036 nerve Anatomy 0.000 description 4
229910052757 nitrogen Inorganic materials 0.000 description 4
-1 poly (oxyphosphinicooxy-1,3-propanediol Chemical class 0.000 description 4
210000004116 schwann cell Anatomy 0.000 description 4
210000003497 sciatic nerve Anatomy 0.000 description 4
238000000926 separation method Methods 0.000 description 4
238000013519 translation Methods 0.000 description 4
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
239000011534 wash buffer Substances 0.000 description 4
229920001817 Agar Polymers 0.000 description 3
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
108091026890 Coding region Proteins 0.000 description 3
KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 3
102000012410 DNA Ligases Human genes 0.000 description 3
108010061982 DNA Ligases Proteins 0.000 description 3
241000196324 Embryophyta Species 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
101000574648 Homo sapiens Retinoid-inducible serine carboxypeptidase Proteins 0.000 description 3
102100034170 Interferon-induced, double-stranded RNA-activated protein kinase Human genes 0.000 description 3
101710089751 Interferon-induced, double-stranded RNA-activated protein kinase Proteins 0.000 description 3
108090000862 Ion Channels Proteins 0.000 description 3
102000004310 Ion Channels Human genes 0.000 description 3
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
102000003939 Membrane transport proteins Human genes 0.000 description 3
108090000301 Membrane transport proteins Proteins 0.000 description 3
241000204031 Mycoplasma Species 0.000 description 3
101710163270 Nuclease Proteins 0.000 description 3
239000004698 Polyethylene Substances 0.000 description 3
238000002123 RNA extraction Methods 0.000 description 3
102100025483 Retinoid-inducible serine carboxypeptidase Human genes 0.000 description 3
240000004808 Saccharomyces cerevisiae Species 0.000 description 3
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
241000282898 Sus scrofa Species 0.000 description 3
239000004480 active ingredient Substances 0.000 description 3
230000001154 acute effect Effects 0.000 description 3
239000008272 agar Substances 0.000 description 3
239000011543 agarose gel Substances 0.000 description 3
206010053552 allodynia Diseases 0.000 description 3
239000012736 aqueous medium Substances 0.000 description 3
230000008901 benefit Effects 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
235000011148 calcium chloride Nutrition 0.000 description 3
HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Substances ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 3
238000011109 contamination Methods 0.000 description 3
238000001816 cooling Methods 0.000 description 3
210000000805 cytoplasm Anatomy 0.000 description 3
230000001419 dependent effect Effects 0.000 description 3
201000010099 disease Diseases 0.000 description 3
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
238000001962 electrophoresis Methods 0.000 description 3
108010048367 enhanced green fluorescent protein Proteins 0.000 description 3
238000011156 evaluation Methods 0.000 description 3
230000002964 excitative effect Effects 0.000 description 3
239000008103 glucose Substances 0.000 description 3
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 3
XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 3
239000001257 hydrogen Substances 0.000 description 3
238000010874 in vitro model Methods 0.000 description 3
238000002955 isolation Methods 0.000 description 3
239000011777 magnesium Substances 0.000 description 3
229910052749 magnesium Inorganic materials 0.000 description 3
229910001629 magnesium chloride Inorganic materials 0.000 description 3
239000003550 marker Substances 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
239000013642 negative control Substances 0.000 description 3
230000003040 nociceptive effect Effects 0.000 description 3
230000001575 pathological effect Effects 0.000 description 3
230000002028 premature Effects 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
239000011535 reaction buffer Substances 0.000 description 3
102000005962 receptors Human genes 0.000 description 3
108020003175 receptors Proteins 0.000 description 3
230000002829 reductive effect Effects 0.000 description 3
230000001105 regulatory effect Effects 0.000 description 3
210000002966 serum Anatomy 0.000 description 3
230000003584 silencer Effects 0.000 description 3
241000894007 species Species 0.000 description 3
238000003756 stirring Methods 0.000 description 3
230000004083 survival effect Effects 0.000 description 3
230000001225 therapeutic effect Effects 0.000 description 3
230000001052 transient effect Effects 0.000 description 3
MGRVRXRGTBOSHW-UHFFFAOYSA-N (aminomethyl)phosphonic acid Chemical compound NCP(O)(O)=O MGRVRXRGTBOSHW-UHFFFAOYSA-N 0.000 description 2
VLEIUWBSEKKKFX-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O VLEIUWBSEKKKFX-UHFFFAOYSA-N 0.000 description 2
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 2
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
108020005544 Antisense RNA Proteins 0.000 description 2
239000004475 Arginine Substances 0.000 description 2
101000714457 Chaetoceros protobacilladnavirus 2 Capsid protein Proteins 0.000 description 2
102000029816 Collagenase Human genes 0.000 description 2
108060005980 Collagenase Proteins 0.000 description 2
UHDGCWIWMRVCDJ-CCXZUQQUSA-N Cytarabine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-CCXZUQQUSA-N 0.000 description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
101710091045 Envelope protein Proteins 0.000 description 2
241000283073 Equus caballus Species 0.000 description 2
241000206602 Eukaryota Species 0.000 description 2
108700024394 Exon Proteins 0.000 description 2
102000018899 Glutamate Receptors Human genes 0.000 description 2
108010027915 Glutamate Receptors Proteins 0.000 description 2
102000009127 Glutaminase Human genes 0.000 description 2
108010073324 Glutaminase Proteins 0.000 description 2
239000012981 Hank's balanced salt solution Substances 0.000 description 2
101000805528 Homo sapiens Vesicular glutamate transporter 2 Proteins 0.000 description 2
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
239000004472 Lysine Substances 0.000 description 2
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
101100428529 Mus musculus Slc17a6 gene Proteins 0.000 description 2
HOKKHZGPKSLGJE-GSVOUGTGSA-N N-Methyl-D-aspartic acid Chemical compound CN[C@@H](C(O)=O)CC(O)=O HOKKHZGPKSLGJE-GSVOUGTGSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
102000016462 Phosphate Transport Proteins Human genes 0.000 description 2
108010092528 Phosphate Transport Proteins Proteins 0.000 description 2
241001505332 Polyomavirus sp. Species 0.000 description 2
101710188315 Protein X Proteins 0.000 description 2
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
238000011530 RNeasy Mini Kit Methods 0.000 description 2
238000011529 RT qPCR Methods 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108091028664 Ribonucleotide Proteins 0.000 description 2
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
102000003565 TRPV2 Human genes 0.000 description 2
241001365914 Taira Species 0.000 description 2
101150077905 Trpv2 gene Proteins 0.000 description 2
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
101710108545 Viral protein 1 Proteins 0.000 description 2
241000700605 Viruses Species 0.000 description 2
208000027418 Wounds and injury Diseases 0.000 description 2
239000000443 aerosol Substances 0.000 description 2
230000003321 amplification Effects 0.000 description 2
238000010171 animal model Methods 0.000 description 2
239000008346 aqueous phase Substances 0.000 description 2
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
210000001130 astrocyte Anatomy 0.000 description 2
210000003050 axon Anatomy 0.000 description 2
230000008827 biological function Effects 0.000 description 2
230000037396 body weight Effects 0.000 description 2
210000004556 brain Anatomy 0.000 description 2
239000002775 capsule Substances 0.000 description 2
210000005056 cell body Anatomy 0.000 description 2
210000001638 cerebellum Anatomy 0.000 description 2
238000012512 characterization method Methods 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
230000004186 co-expression Effects 0.000 description 2
238000012761 co-transfection Methods 0.000 description 2
239000011248 coating agent Substances 0.000 description 2
238000000576 coating method Methods 0.000 description 2
229960002424 collagenase Drugs 0.000 description 2
210000004748 cultured cell Anatomy 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
238000013461 design Methods 0.000 description 2
238000011496 digital image analysis Methods 0.000 description 2
BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 2
238000006073 displacement reaction Methods 0.000 description 2
238000009826 distribution Methods 0.000 description 2
238000001035 drying Methods 0.000 description 2
239000000975 dye Substances 0.000 description 2
238000004520 electroporation Methods 0.000 description 2
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
229960005542 ethidium bromide Drugs 0.000 description 2
239000012091 fetal bovine serum Substances 0.000 description 2
235000011389 fruit/vegetable juice Nutrition 0.000 description 2
239000008187 granular material Substances 0.000 description 2
239000003102 growth factor Substances 0.000 description 2
102000045564 human SLC17A6 Human genes 0.000 description 2
210000005260 human cell Anatomy 0.000 description 2
238000010348 incorporation Methods 0.000 description 2
230000001939 inductive effect Effects 0.000 description 2
208000014674 injury Diseases 0.000 description 2
238000009434 installation Methods 0.000 description 2
210000002414 leg Anatomy 0.000 description 2
239000003446 ligand Substances 0.000 description 2
238000002156 mixing Methods 0.000 description 2
230000036651 mood Effects 0.000 description 2
210000001640 nerve ending Anatomy 0.000 description 2
210000000944 nerve tissue Anatomy 0.000 description 2
210000000653 nervous system Anatomy 0.000 description 2
208000004296 neuralgia Diseases 0.000 description 2
208000021722 neuropathic pain Diseases 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
238000000399 optical microscopy Methods 0.000 description 2
238000005457 optimization Methods 0.000 description 2
150000007530 organic bases Chemical class 0.000 description 2
230000002018 overexpression Effects 0.000 description 2
239000002245 particle Substances 0.000 description 2
230000035515 penetration Effects 0.000 description 2
WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 2
230000002263 peptidergic effect Effects 0.000 description 2
230000002093 peripheral effect Effects 0.000 description 2
239000012071 phase Substances 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
239000004033 plastic Substances 0.000 description 2
229920003023 plastic Polymers 0.000 description 2
230000032361 posttranscriptional gene silencing Effects 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
210000002248 primary sensory neuron Anatomy 0.000 description 2
ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 description 2
150000003212 purines Chemical class 0.000 description 2
238000003908 quality control method Methods 0.000 description 2
230000004044 response Effects 0.000 description 2
238000010839 reverse transcription Methods 0.000 description 2
239000002336 ribonucleotide Substances 0.000 description 2
125000002652 ribonucleotide group Chemical group 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
239000012723 sample buffer Substances 0.000 description 2
230000001953 sensory effect Effects 0.000 description 2
210000000130 stem cell Anatomy 0.000 description 2
235000000346 sugar Nutrition 0.000 description 2
230000000946 synaptic effect Effects 0.000 description 2
210000002504 synaptic vesicle Anatomy 0.000 description 2
239000003826 tablet Substances 0.000 description 2
238000010361 transduction Methods 0.000 description 2
230000026683 transduction Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
230000032258 transport Effects 0.000 description 2
210000004881 tumor cell Anatomy 0.000 description 2
239000013603 viral vector Substances 0.000 description 2
239000012224 working solution Substances 0.000 description 2
101150072531 10 gene Proteins 0.000 description 1
VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 description 1
OVONXEQGWXGFJD-UHFFFAOYSA-N 4-sulfanylidene-1h-pyrimidin-2-one Chemical compound SC=1C=CNC(=O)N=1 OVONXEQGWXGFJD-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
108020004491 Antisense DNA Proteins 0.000 description 1
108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
108020000946 Bacterial DNA Proteins 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
241000167854 Bourreria succulenta Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
102400000967 Bradykinin Human genes 0.000 description 1
101800004538 Bradykinin Proteins 0.000 description 1
241000208199 Buxus sempervirens Species 0.000 description 1
241000283707 Capra Species 0.000 description 1
201000009030 Carcinoma Diseases 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
241000282693 Cercopithecidae Species 0.000 description 1
VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
230000004544 DNA amplification Effects 0.000 description 1
101710200526 DNA polymerase 2 Proteins 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
102000037087 Excitatory amino acid transporters Human genes 0.000 description 1
108091006291 Excitatory amino acid transporters Proteins 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 1
241000233866 Fungi Species 0.000 description 1
102000034575 Glutamate transporters Human genes 0.000 description 1
108091006151 Glutamate transporters Proteins 0.000 description 1
QXZGBUJJYSLZLT-UHFFFAOYSA-N H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH Natural products NC(N)=NCCCC(N)C(=O)N1CCCC1C(=O)N1C(C(=O)NCC(=O)NC(CC=2C=CC=CC=2)C(=O)NC(CO)C(=O)N2C(CCC2)C(=O)NC(CC=2C=CC=CC=2)C(=O)NC(CCCN=C(N)N)C(O)=O)CCC1 QXZGBUJJYSLZLT-UHFFFAOYSA-N 0.000 description 1
238000012404 In vitro experiment Methods 0.000 description 1
108010063738 Interleukins Proteins 0.000 description 1
102000015696 Interleukins Human genes 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
239000007836 KH2PO4 Substances 0.000 description 1
YQEZLKZALYSWHR-UHFFFAOYSA-N Ketamine Chemical compound C=1C=CC=C(Cl)C=1C1(NC)CCCCC1=O YQEZLKZALYSWHR-UHFFFAOYSA-N 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
108091027974 Mature messenger RNA Proteins 0.000 description 1
108700011259 MicroRNAs Proteins 0.000 description 1
241000204003 Mycoplasmatales Species 0.000 description 1
102000004868 N-Methyl-D-Aspartate Receptors Human genes 0.000 description 1
108090001041 N-Methyl-D-Aspartate Receptors Proteins 0.000 description 1
208000028389 Nerve injury Diseases 0.000 description 1
208000001294 Nociceptive Pain Diseases 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
239000012124 Opti-MEM Substances 0.000 description 1
229930040373 Paraformaldehyde Natural products 0.000 description 1
241001494479 Pecora Species 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
108010013639 Peptidoglycan Proteins 0.000 description 1
241000009328 Perro Species 0.000 description 1
BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
102000001708 Protein Isoforms Human genes 0.000 description 1
108010029485 Protein Isoforms Proteins 0.000 description 1
101710149951 Protein Tat Proteins 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 1
CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
238000012181 QIAquick gel extraction kit Methods 0.000 description 1
102000009572 RNA Polymerase II Human genes 0.000 description 1
108010009460 RNA Polymerase II Proteins 0.000 description 1
102000014450 RNA Polymerase III Human genes 0.000 description 1
108010078067 RNA Polymerase III Proteins 0.000 description 1
238000010802 RNA extraction kit Methods 0.000 description 1
239000012980 RPMI-1640 medium Substances 0.000 description 1
108010057163 Ribonuclease III Proteins 0.000 description 1
102000003661 Ribonuclease III Human genes 0.000 description 1
PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
102000034553 SLC17 Human genes 0.000 description 1
108091006169 SLC17 Proteins 0.000 description 1
108091006282 SLC17A8 Proteins 0.000 description 1
101100369153 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) tfbA gene Proteins 0.000 description 1
101100369160 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) tfbB gene Proteins 0.000 description 1
108091081021 Sense strand Proteins 0.000 description 1
206010070834 Sensitisation Diseases 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
208000006097 Spinal Dysraphism Diseases 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
208000010513 Stupor Diseases 0.000 description 1
239000008049 TAE buffer Substances 0.000 description 1
102000003563 TRPV Human genes 0.000 description 1
108060008564 TRPV Proteins 0.000 description 1
102100029621 Transient receptor potential cation channel subfamily V member 2 Human genes 0.000 description 1
102000004243 Tubulin Human genes 0.000 description 1
108090000704 Tubulin Proteins 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
102100038036 Vesicular glutamate transporter 2 Human genes 0.000 description 1
108010003533 Viral Envelope Proteins Proteins 0.000 description 1
206010048010 Withdrawal syndrome Diseases 0.000 description 1
229960000583 acetic acid Drugs 0.000 description 1
HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
230000009471 action Effects 0.000 description 1
239000013543 active substance Substances 0.000 description 1
208000005298 acute pain Diseases 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
230000001464 adherent effect Effects 0.000 description 1
210000001943 adrenal medulla Anatomy 0.000 description 1
HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
230000003574 anti-allodynic effect Effects 0.000 description 1
239000000427 antigen Substances 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
239000003816 antisense DNA Substances 0.000 description 1
239000000074 antisense oligonucleotide Substances 0.000 description 1
238000012230 antisense oligonucleotides Methods 0.000 description 1
230000006907 apoptotic process Effects 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000000823 artificial membrane Substances 0.000 description 1
238000000376 autoradiography Methods 0.000 description 1
210000003719 b-lymphocyte Anatomy 0.000 description 1
230000006399 behavior Effects 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
239000011230 binding agent Substances 0.000 description 1
239000012148 binding buffer Substances 0.000 description 1
230000008436 biogenesis Effects 0.000 description 1
238000010170 biological method Methods 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
TXFLGZOGNOOEFZ-UHFFFAOYSA-N bis(2-chloroethyl)amine Chemical compound ClCCNCCCl TXFLGZOGNOOEFZ-UHFFFAOYSA-N 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
230000008499 blood brain barrier function Effects 0.000 description 1
230000017531 blood circulation Effects 0.000 description 1
210000004204 blood vessel Anatomy 0.000 description 1
210000001218 blood-brain barrier Anatomy 0.000 description 1
238000006664 bond formation reaction Methods 0.000 description 1
QXZGBUJJYSLZLT-FDISYFBBSA-N bradykinin Chemical compound NC(=N)NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(=O)NCC(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CO)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)CCC1 QXZGBUJJYSLZLT-FDISYFBBSA-N 0.000 description 1
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
239000012876 carrier material Substances 0.000 description 1
230000021164 cell adhesion Effects 0.000 description 1
230000030833 cell death Effects 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
238000002144 chemical decomposition reaction Methods 0.000 description 1
125000003636 chemical group Chemical group 0.000 description 1
235000019693 cherries Nutrition 0.000 description 1
230000008045 co-localization Effects 0.000 description 1
239000005515 coenzyme Substances 0.000 description 1
230000001149 cognitive effect Effects 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
210000002808 connective tissue Anatomy 0.000 description 1
239000007799 cork Substances 0.000 description 1
230000001054 cortical effect Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 description 1
229940099500 cystamine Drugs 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
210000000172 cytosol Anatomy 0.000 description 1
231100000433 cytotoxic Toxicity 0.000 description 1
230000001472 cytotoxic effect Effects 0.000 description 1
230000009849 deactivation Effects 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000007850 degeneration Effects 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
210000001787 dendrite Anatomy 0.000 description 1
230000002999 depolarising effect Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
230000004069 differentiation Effects 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
238000002224 dissection Methods 0.000 description 1
239000008298 dragée Substances 0.000 description 1
239000006196 drop Substances 0.000 description 1
102000010982 eIF-2 Kinase Human genes 0.000 description 1
108010037623 eIF-2 Kinase Proteins 0.000 description 1
239000012636 effector Substances 0.000 description 1
230000002996 emotional effect Effects 0.000 description 1
210000003890 endocrine cell Anatomy 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
239000003797 essential amino acid Substances 0.000 description 1
235000020776 essential amino acid Nutrition 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
230000002461 excitatory amino acid Effects 0.000 description 1
239000003257 excitatory amino acid Substances 0.000 description 1
239000013604 expression vector Substances 0.000 description 1
210000001723 extracellular space Anatomy 0.000 description 1
239000003925 fat Substances 0.000 description 1
239000000835 fiber Substances 0.000 description 1
210000002950 fibroblast Anatomy 0.000 description 1
239000000945 filler Substances 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000012530 fluid Substances 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
239000012595 freezing medium Substances 0.000 description 1
239000012362 glacial acetic acid Substances 0.000 description 1
230000000848 glutamatergic effect Effects 0.000 description 1
125000003827 glycol group Chemical group 0.000 description 1
210000004884 grey matter Anatomy 0.000 description 1
230000012010 growth Effects 0.000 description 1
230000035876 healing Effects 0.000 description 1
230000003862 health status Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
210000001320 hippocampus Anatomy 0.000 description 1
238000002744 homologous recombination Methods 0.000 description 1
230000006801 homologous recombination Effects 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
238000010191 image analysis Methods 0.000 description 1
238000003384 imaging method Methods 0.000 description 1
230000007124 immune defense Effects 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
238000003365 immunocytochemistry Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
210000004969 inflammatory cell Anatomy 0.000 description 1
230000004941 influx Effects 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
239000000138 intercalating agent Substances 0.000 description 1
230000010468 interferon response Effects 0.000 description 1
229940047122 interleukins Drugs 0.000 description 1
210000001153 interneuron Anatomy 0.000 description 1
244000000056 intracellular parasite Species 0.000 description 1
238000007912 intraperitoneal administration Methods 0.000 description 1
238000011835 investigation Methods 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
230000003902 lesion Effects 0.000 description 1
210000003041 ligament Anatomy 0.000 description 1
125000005647 linker group Chemical group 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
238000001638 lipofection Methods 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
238000002483 medication Methods 0.000 description 1
229910021645 metal ion Inorganic materials 0.000 description 1
MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 description 1
239000002679 microRNA Substances 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
230000003278 mimic effect Effects 0.000 description 1
230000011278 mitosis Effects 0.000 description 1
238000004264 monolayer culture Methods 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
210000004400 mucous membrane Anatomy 0.000 description 1
229940105631 nembutal Drugs 0.000 description 1
230000008764 nerve damage Effects 0.000 description 1
210000004126 nerve fiber Anatomy 0.000 description 1
239000002858 neurotransmitter agent Substances 0.000 description 1
230000036441 nociceptive stimulation Effects 0.000 description 1
230000001473 noxious effect Effects 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
210000004248 oligodendroglia Anatomy 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
210000003463 organelle Anatomy 0.000 description 1
150000002891 organic anions Chemical class 0.000 description 1
239000012074 organic phase Substances 0.000 description 1
230000002611 ovarian Effects 0.000 description 1
229940094443 oxytocics prostaglandins Drugs 0.000 description 1
229940124583 pain medication Drugs 0.000 description 1
230000008052 pain pathway Effects 0.000 description 1
239000012188 paraffin wax Substances 0.000 description 1
229920002866 paraformaldehyde Polymers 0.000 description 1
244000045947 parasite Species 0.000 description 1
230000003071 parasitic effect Effects 0.000 description 1
230000001936 parietal effect Effects 0.000 description 1
244000052769 pathogen Species 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
229960001412 pentobarbital Drugs 0.000 description 1
150000002972 pentoses Chemical class 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
210000002951 peptidergic neuron Anatomy 0.000 description 1
230000035699 permeability Effects 0.000 description 1
230000002085 persistent effect Effects 0.000 description 1
239000000825 pharmaceutical preparation Substances 0.000 description 1
238000011458 pharmacological treatment Methods 0.000 description 1
229960003531 phenolsulfonphthalein Drugs 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
125000001863 phosphorothioyl group Chemical group *P(*)(*)=S 0.000 description 1
125000005642 phosphothioate group Chemical group 0.000 description 1
238000005375 photometry Methods 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
230000008488 polyadenylation Effects 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
229920001296 polysiloxane Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
230000009023 proprioceptive sensation Effects 0.000 description 1
150000003180 prostaglandins Chemical class 0.000 description 1
230000001681 protective effect Effects 0.000 description 1
238000011002 quantification Methods 0.000 description 1
239000010453 quartz Substances 0.000 description 1
238000011552 rat model Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000011514 reflex Effects 0.000 description 1
230000002787 reinforcement Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
238000003757 reverse transcription PCR Methods 0.000 description 1
230000008313 sensitization Effects 0.000 description 1
210000000697 sensory organ Anatomy 0.000 description 1
230000020341 sensory perception of pain Effects 0.000 description 1
239000000741 silica gel Substances 0.000 description 1
229910002027 silica gel Inorganic materials 0.000 description 1
239000004055 small Interfering RNA Substances 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
230000008925 spontaneous activity Effects 0.000 description 1
238000012453 sprague-dawley rat model Methods 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
239000007858 starting material Substances 0.000 description 1
230000000638 stimulation Effects 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
238000003860 storage Methods 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
239000013589 supplement Substances 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
230000009782 synaptic response Effects 0.000 description 1
235000020357 syrup Nutrition 0.000 description 1
239000006188 syrup Substances 0.000 description 1
230000008467 tissue growth Effects 0.000 description 1
208000037816 tissue injury Diseases 0.000 description 1
238000004448 titration Methods 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
231100000041 toxicology testing Toxicity 0.000 description 1
230000010474 transient expression Effects 0.000 description 1
108091005703 transmembrane proteins Proteins 0.000 description 1
102000035160 transmembrane proteins Human genes 0.000 description 1
230000001960 triggered effect Effects 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
230000007306 turnover Effects 0.000 description 1
241000701161 unidentified adenovirus Species 0.000 description 1
210000001170 unmyelinated nerve fiber Anatomy 0.000 description 1
210000000689 upper leg Anatomy 0.000 description 1
229940035893 uracil Drugs 0.000 description 1
238000009423 ventilation Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
238000005303 weighing Methods 0.000 description 1
BPICBUSOMSTKRF-UHFFFAOYSA-N xylazine Chemical compound CC1=CC=CC(C)=C1NC1=NCCCS1 BPICBUSOMSTKRF-UHFFFAOYSA-N 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

ES05707623T 2004-03-10 2005-02-24 Compuestos de arn de doble hebra especificos de vglut. Expired - Lifetime ES2347574T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE102004011687A DE102004011687A1 (de)	2004-03-10	2004-03-10	VGLUT-spezifische dsRNA-Verbindungen
DE102004011687		2004-03-10

Publications (1)

Publication Number	Publication Date
ES2347574T3 true ES2347574T3 (es)	2010-11-02

Family

ID=34960530

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES05707623T Expired - Lifetime ES2347574T3 (es)	2004-03-10	2005-02-24	Compuestos de arn de doble hebra especificos de vglut.

Country Status (7)

Country	Link
US (1)	US20070117771A1 (de)
EP (1)	EP1723234B1 (de)
AT (1)	ATE471375T1 (de)
DE (2)	DE102004011687A1 (de)
ES (1)	ES2347574T3 (de)
PL (1)	PL1723234T3 (de)
WO (1)	WO2005090571A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE10356113A1 (de)	2003-11-27	2005-06-23	Behr Gmbh & Co. Kg	Verfahren zur Herstellung eines Werkstücks
CN112760276A (zh) *	2021-03-24	2021-05-07	扬州大学	一种柑橘原生质体的制备方法
CN114457045B (zh) *	2022-02-25	2023-07-14	中国人民解放军军事科学院军事医学研究院	抑制Slc2a1的RNAi腺相关病毒及其制备和应用
CN120366312A (zh) *	2025-06-24	2025-07-25	南京农业大学三亚研究院	一种用于防治二化螟的靶向囊泡型谷氨酸转运体的dsRNA制剂与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6818391B2 (en) *	2000-07-25	2004-11-16	The Regents Of The University Of California	Glutamate transporters
DE10128541A1 (de) *	2001-06-13	2003-02-20	Gruenenthal Gmbh	Screening-Verfahren mit BNPI und DNPI
DE10133915A1 (de) *	2001-07-12	2003-02-06	Aventis Pharma Gmbh	Neue Oligoribonucleotid-Derivate zur gezielten Hemmung der Genexpression

2004
- 2004-03-10 DE DE102004011687A patent/DE102004011687A1/de not_active Withdrawn
2005
- 2005-02-24 DE DE502005009757T patent/DE502005009757D1/de not_active Expired - Lifetime
- 2005-02-24 WO PCT/EP2005/001970 patent/WO2005090571A1/de not_active Ceased
- 2005-02-24 EP EP05707623A patent/EP1723234B1/de not_active Expired - Lifetime
- 2005-02-24 AT AT05707623T patent/ATE471375T1/de active
- 2005-02-24 ES ES05707623T patent/ES2347574T3/es not_active Expired - Lifetime
- 2005-02-24 PL PL05707623T patent/PL1723234T3/pl unknown
2006
- 2006-09-11 US US11/518,284 patent/US20070117771A1/en not_active Abandoned

Also Published As

Publication number	Publication date
US20070117771A1 (en)	2007-05-24
WO2005090571A1 (de)	2005-09-29
DE102004011687A1 (de)	2005-10-13
DE502005009757D1 (de)	2010-07-29
EP1723234A1 (de)	2006-11-22
EP1723234B1 (de)	2010-06-16
PL1723234T3 (pl)	2010-11-30
ATE471375T1 (de)	2010-07-15

Publication	Publication Date	Title
JP6845589B2 (ja)	2021-03-17	腱治癒を調節するための物質および方法
US20110165227A1 (en)	2011-07-07	Compositions and Methods Using siRNA Molecules for Treatment of Gliomas
US8163709B2 (en)	2012-04-24	TAK1-D mediated induction of cell death in human cancer cells by specific sequence short double-stranded RNAS
JP2017536826A (ja)	2017-12-14	幹細胞および／または前駆細胞からの赤血球産生を改善するためのｓｈ２ｂ３の調節
Simmons et al.	2016	Retroviral transduction of T cells and T cell precursors
EP3359553A1 (de)	2018-08-15	Zusammensetzungen und verfahren zur behandlung von diabetischer retinopathie
CN103380214B (zh)	2015-07-15	用于治疗脑肿瘤的支链氨基转移酶-1(bcat1)抑制剂
ES2347574T3 (es)	2010-11-02	Compuestos de arn de doble hebra especificos de vglut.
ES2559106B1 (es)	2016-11-25	Método de activación de la expresión del gen Pitx2 para promover la regeneración muscular
CN101213300B (zh)	2013-01-23	用于rna干扰的寡核苷酸及其生物学应用
CA2672937A1 (en)	2008-06-26	Inhibitory polynucleotide compositions and methods for treating cancer
AU2009303355B2 (en)	2015-10-01	FAS/FASL or other death receptor targeted methods and compositions for killing tumor cells
US20230092787A1 (en)	2023-03-23	Car t cells targeting the integrin alphav beta3 exhibit robust anti-tumor responses against gliomas and other solid tumor malignancies
US20150329857A1 (en)	2015-11-19	Rna interference to activate stem cells
US20150283164A1 (en)	2015-10-08	Treatment of Myelodysplastic Syndrome by Inhibition of NR2F2
KR20140122380A (ko)	2014-10-20	Ｔｃｔｐ-ｐｔｄ를 포함하는 유전자 전달체
Yokota	0	32. OCs induced Ki-67-negative population in leukemic cells We next sought to determine the source of growth suppression of Ba/F3 wt bcr-abl cells in the OB+ OC+ bone co-culture. First, we examined the cell cycle status of Ba/F3 wt bcr-abl cells co-cultured with OBs, OCs and/or bone slices. Ki-57 is expressed throughout