ES2375860B8 - Método para la obtención de células precursoras de oligodendrocitos. - Google Patents

Método para la obtención de células precursoras de oligodendrocitos. Download PDF

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Publication number
ES2375860B8
ES2375860B8 ES201030493A ES201030493A ES2375860B8 ES 2375860 B8 ES2375860 B8 ES 2375860B8 ES 201030493 A ES201030493 A ES 201030493A ES 201030493 A ES201030493 A ES 201030493A ES 2375860 B8 ES2375860 B8 ES 2375860B8
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ES
Spain
Prior art keywords
obtaining
precursor cells
pos
oligodendrocit
cerebral cortex
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201030493A
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English (en)
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ES2375860B1 (es
ES2375860A1 (es
Inventor
Fernando De Castro Soubriet
Francisco Javier Arenzana Sanagérico
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Fundacion Hospital Nacional de Paraplejicos Para la Investigacion Y la Integracion (FUHNPAIIN)
Original Assignee
Fundacion Hospital Nacional de Paraplejicos Para la Investigacion Y la Integracion (FUHNPAIIN)
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Application filed by Fundacion Hospital Nacional de Paraplejicos Para la Investigacion Y la Integracion (FUHNPAIIN) filed Critical Fundacion Hospital Nacional de Paraplejicos Para la Investigacion Y la Integracion (FUHNPAIIN)
Priority to ES201030493A priority Critical patent/ES2375860B8/es
Priority to EP11721797A priority patent/EP2554663A2/en
Priority to PCT/ES2011/070191 priority patent/WO2011121158A2/es
Publication of ES2375860A1 publication Critical patent/ES2375860A1/es
Publication of ES2375860B1 publication Critical patent/ES2375860B1/es
Application granted granted Critical
Publication of ES2375860B8 publication Critical patent/ES2375860B8/es
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Método para la obtención de células precursoras de oligodendrocitos.#Método para la purificación de células precursoras de oligodendrocitos (POs) de corteza cerebral a partir de la corteza cerebral de un mamífero de edad superior a una semana, que comprende: obtener una muestra aislada de cerebro; seleccionar las POs de la muestra biológica obtenida anteriormente y cultivar las POs en condiciones de proliferación, migración, movilidad o diferenciación celular. Además, la presente invención se refiere a los Pos obtenidos por el método de la invención y a las líneas celulares derivadas del mismo.
ES201030493A 2010-03-31 2010-03-31 Método para la obtención de células precursoras de oligodendrocitos. Expired - Fee Related ES2375860B8 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES201030493A ES2375860B8 (es) 2010-03-31 2010-03-31 Método para la obtención de células precursoras de oligodendrocitos.
EP11721797A EP2554663A2 (en) 2010-03-31 2011-03-22 Method for obtaining oligodendrocyte precursor cells
PCT/ES2011/070191 WO2011121158A2 (es) 2010-03-31 2011-03-22 Método para la obtención de células precursoras de oligodendrocitos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201030493A ES2375860B8 (es) 2010-03-31 2010-03-31 Método para la obtención de células precursoras de oligodendrocitos.

Publications (3)

Publication Number Publication Date
ES2375860A1 ES2375860A1 (es) 2012-03-07
ES2375860B1 ES2375860B1 (es) 2013-01-25
ES2375860B8 true ES2375860B8 (es) 2013-06-10

Family

ID=44484795

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201030493A Expired - Fee Related ES2375860B8 (es) 2010-03-31 2010-03-31 Método para la obtención de células precursoras de oligodendrocitos.

Country Status (3)

Country Link
EP (1) EP2554663A2 (es)
ES (1) ES2375860B8 (es)
WO (1) WO2011121158A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115340982A (zh) * 2022-08-23 2022-11-15 徐州医科大学 一种分离并纯化新生小鼠皮质区少突胶质前体细胞的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007200A1 (en) 1995-08-16 1997-02-27 The Board Of Trustees Of The Leland Stanford Junior University Adult oligodendrocyte precursor cell compositions and methods
AR046076A1 (es) * 2003-07-18 2005-11-23 Otsuka Pharma Co Ltd Procedimiento para obtener una poblacion homogenea de celulas precursoras de oligodendrocitos y poblacion obtenida
EP1684706A4 (en) * 2003-11-10 2008-01-09 Cornell Res Foundation Inc GENES DIFFERENTIALLY EXPRESSED BY INSULATED PRECURSOR CELLS OF HUMAN WHITE MATTER
US20060183147A1 (en) * 2005-02-11 2006-08-17 Elan Pharmaceuticals, Inc. Methods of identifying genes which modulate myelination

Also Published As

Publication number Publication date
WO2011121158A3 (es) 2011-12-08
ES2375860B1 (es) 2013-01-25
EP2554663A2 (en) 2013-02-06
WO2011121158A2 (es) 2011-10-06
ES2375860A1 (es) 2012-03-07

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