ES2422424T3 - Serina recombinasas específicas de sitio y métodos para su uso - Google Patents

Serina recombinasas específicas de sitio y métodos para su uso

Info

Publication number
ES2422424T3
ES2422424T3 ES05722807T ES05722807T ES2422424T3 ES 2422424 T3 ES2422424 T3 ES 2422424T3 ES 05722807 T ES05722807 T ES 05722807T ES 05722807 T ES05722807 T ES 05722807T ES 2422424 T3 ES2422424 T3 ES 2422424T3
Authority
ES
Spain
Prior art keywords
recombination
sites
recombinase
site
attp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES05722807T
Other languages
English (en)
Inventor
Malla Padidam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precigen Inc
Original Assignee
Intrexon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corp filed Critical Intrexon Corp
Application granted granted Critical
Publication of ES2422424T3 publication Critical patent/ES2422424T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A61P31/06—Antibacterial agents for tuberculosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A61P33/06—Antimalarials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/22—Endodeoxyribonucleases producing 3'-phosphomonoesters (3.1.22)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Oncology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Diabetes (AREA)
  • Rheumatology (AREA)
  • Pulmonology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Pain & Pain Management (AREA)

Abstract

Un método para obtener una recombinación específica de sitio en una célula eucariota aislada, comprendiendo elmétodo: proporcionar una célula eucariota aislada que comprende un primer sitio de recombinación y un segundositio de recombinación con una secuencia de polinucleótidos flanqueada por un tercer sitio de recombinación y uncuarto sitio de recombinación; poner en contacto los sitios de recombinación con un polipéptido de recombinasaprocariota, que tiene como resultado la recombinación entre los sitios de recombinación, en el que el polipéptido derecombinasa puede participar en la recombinación entre el primero y el tercer sitios de recombinación y el segundo yel cuarto sitios de recombinación, el primero y el segundo sitios de recombinación son attP o attB, el tercero y elcuarto sitios de recombinación son attB o attP y la recombinasa es una recombinasa del fago de Mycobacteriumtuberculosis y una recombinasa del fago de Mycobacterium smegmatis, en el que la recombinasa se selecciona delgrupo que consiste en una recombinasa fRv1 y una recombinasa Bxb1, siempre que cuando el primero y el segundositios de unión para recombinación son attB, el tercero y el cuarto sitios de unión para recombinación son attP ycuando el primero y el segundo sitios de unión para recombinación son attP el tercero y el cuarto sitios de unión pararecombinación son attB
ES05722807T 2005-02-02 2005-02-08 Serina recombinasas específicas de sitio y métodos para su uso Expired - Lifetime ES2422424T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/049,552 US9034650B2 (en) 2005-02-02 2005-02-02 Site-specific serine recombinases and methods of their use
PCT/US2005/003851 WO2006083253A1 (en) 2005-02-02 2005-02-08 Site-specific serine recombinases and methods of their use

Publications (1)

Publication Number Publication Date
ES2422424T3 true ES2422424T3 (es) 2013-09-11

Family

ID=36757054

Family Applications (2)

Application Number Title Priority Date Filing Date
ES10181444.0T Expired - Lifetime ES2584911T3 (es) 2005-02-02 2005-02-08 Serina recombinasas específicas de sitio y métodos para su uso
ES05722807T Expired - Lifetime ES2422424T3 (es) 2005-02-02 2005-02-08 Serina recombinasas específicas de sitio y métodos para su uso

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES10181444.0T Expired - Lifetime ES2584911T3 (es) 2005-02-02 2005-02-08 Serina recombinasas específicas de sitio y métodos para su uso

Country Status (15)

Country Link
US (5) US9034650B2 (es)
EP (3) EP1844155B1 (es)
JP (4) JP5684445B2 (es)
KR (1) KR20080003780A (es)
CN (2) CN102994492B (es)
AU (1) AU2005326917B2 (es)
BR (1) BRPI0519865A2 (es)
CA (4) CA2595929C (es)
DK (2) DK2327786T3 (es)
ES (2) ES2584911T3 (es)
IL (3) IL185027A (es)
MX (1) MX2007009349A (es)
NZ (2) NZ583562A (es)
SG (1) SG10201509276YA (es)
WO (1) WO2006083253A1 (es)

Families Citing this family (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040033600A1 (en) 2001-03-21 2004-02-19 Palli Subba Reddy Ecdysone receptor-based inducible gene expression system
US8105825B2 (en) 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
CA2438119C (en) 2001-02-20 2014-12-16 Rheogene Holdings, Inc. Chimeric retinoid x receptors and their use in a novel ecdysone receptor-based inducible gene expression system
EP1534738B1 (en) 2001-02-20 2012-06-20 Intrexon Corporation Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
AU2002306550B2 (en) 2001-02-20 2007-10-25 Intrexon Corporation Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
ES2385598T3 (es) 2001-02-20 2012-07-27 Intrexon Corporation Sistema novedoso de expresión génica inducible basado en el receptor de ecdisona/receptor X retinoide de invertebrado
JP2005532781A (ja) 2001-09-26 2005-11-04 レオジーン・ホールディングス，インコーポレーテッド ヨコバイエクジソン受容体核酸、ポリペプチド、およびそれらの使用
US8304233B2 (en) * 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US7304161B2 (en) 2003-02-10 2007-12-04 Intrexon Corporation Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
US7456315B2 (en) 2003-02-28 2008-11-25 Intrexon Corporation Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US7935510B2 (en) 2004-04-30 2011-05-03 Intrexon Corporation Mutant receptors and their use in a nuclear receptor-based inducible gene expression system
EP2865432B1 (en) 2004-06-29 2020-09-09 Donaldson Company, Inc. Liquid filter arrangement
US9034650B2 (en) 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
US20090093403A1 (en) 2007-03-01 2009-04-09 Feng Zhang Systems, methods and compositions for optical stimulation of target cells
US10052497B2 (en) * 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
JP2009502140A (ja) * 2005-07-22 2009-01-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 光活性化陽イオンチャネルおよびその使用
US8926959B2 (en) 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US9238150B2 (en) * 2005-07-22 2016-01-19 The Board Of Trustees Of The Leland Stanford Junior University Optical tissue interface method and apparatus for stimulating cells
US9274099B2 (en) 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
EP2019860A4 (en) * 2006-05-22 2010-12-15 Hiprocell Llc PROTEIN PRODUCTION USING EUKARYOTIC CELL LINES
US8398692B2 (en) 2007-01-10 2013-03-19 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US8401609B2 (en) * 2007-02-14 2013-03-19 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US8455628B2 (en) * 2007-05-02 2013-06-04 Intrexon Corporation PP1 ligands
US20090098055A1 (en) 2007-08-23 2009-04-16 Intrexon Corporation Methods and Compositions for Diagnosing Disease
DK2205618T3 (en) 2007-09-26 2017-02-20 Intrexon Corp SYNTHETIC 5 'NON-TRANSLATED REGIONS, EXPRESSION VECTORS AND PROCEDURES FOR INCREASING TRANSGENIC EXPRESSION
US20090136465A1 (en) 2007-09-28 2009-05-28 Intrexon Corporation Therapeutic Gene-Switch Constructs and Bioreactors for the Expression of Biotherapeutic Molecules, and Uses Thereof
JP2010539993A (ja) 2007-10-08 2010-12-24 イントレキソン コーポレーション 遺伝子操作した樹状細胞および癌の治療のための使用
US10434327B2 (en) * 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US10035027B2 (en) * 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
ES2701349T3 (es) 2008-04-23 2019-02-21 Univ Leland Stanford Junior Sistemas, métodos y composiciones para la estimulación óptica de células diana
EP2294208B1 (en) 2008-05-29 2013-05-08 The Board of Trustees of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
EP2303405A4 (en) * 2008-06-17 2017-12-27 The Board of Trustees of the Leland Stanford Junior University Apparatus and methods for controlling cellular development
JP2011525130A (ja) 2008-06-17 2011-09-15 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 光伝送素子を用いた標的細胞の光刺激のための方法、システム、および装置
WO2010006049A1 (en) 2008-07-08 2010-01-14 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
US20110223635A1 (en) * 2008-08-11 2011-09-15 Karl Deisseroth Method and Composition for Controlling Gene Expression
NZ602416A (en) 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto
EP3399024A1 (en) 2010-03-17 2018-11-07 The Board of Trustees of The Leland Stanford Junior University Light-sensitive ion-passing molecules
EP2550359B1 (en) 2010-03-23 2017-08-09 Intrexon Corporation Vectors conditionally expressing therapeutic proteins, host cells comprising the vectors, and uses thereof
EP3486253A1 (en) 2010-11-05 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US10568307B2 (en) 2010-11-05 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
CN103384469B (zh) 2010-11-05 2016-06-15 斯坦福大学托管董事会 光控cns功能障碍
CN106422081B (zh) 2010-11-05 2019-06-21 斯坦福大学托管董事会 用于光遗传学方法的光的上转换
US10086012B2 (en) 2010-11-05 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
ES2625179T3 (es) 2010-11-05 2017-07-18 The Board Of Trustees Of The Leland Stanford Junior University Control optogenético de comportamientos relacionados con la recompensa
US8696722B2 (en) 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US20120141441A1 (en) * 2010-12-03 2012-06-07 The Board Of Trustees Of The Leland Stanford Junior University Methods and Compositions for Treatment of Muscular Dystrophy
EP2681327B1 (en) 2011-03-04 2018-11-21 Intrexon Corporation Vectors conditionally expressing protein
CA2859364C (en) 2011-12-16 2019-05-07 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
ES2628006T3 (es) 2012-02-21 2017-08-01 Circuit Therapeutics, Inc. Composiciones para el tratamiento de trastornos neurogénicos del suelo pélvico
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
ES2683329T3 (es) 2013-03-15 2018-09-26 Intrexon Corporation Diacilhidrazinas que contienen boro
ES2742492T3 (es) 2013-03-15 2020-02-14 Univ Leland Stanford Junior Control optogenético del estado conductual
US10220092B2 (en) 2013-04-29 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Devices, systems and methods for optogenetic modulation of action potentials in target cells
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
JP6621747B2 (ja) 2013-08-14 2019-12-18 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 疼痛を制御するための組成物及び方法
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9932607B2 (en) 2013-11-15 2018-04-03 The Board Of Trustees Of The Leland Stanford Junior University Site-specific integration of transgenes into human cells
US9683280B2 (en) 2014-01-10 2017-06-20 Ut-Battelle, Llc Intermediate strength alloys for high temperature service in liquid-salt cooled energy systems
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016025719A1 (en) * 2014-08-15 2016-02-18 Massachusetts Institute Of Technology Genomically-encoded memory in live cells
EP3194359B1 (en) 2014-09-17 2020-04-08 Intrexon Corporation Boron-containing diacylhydrazine compounds
MA41644A (fr) 2015-03-03 2018-01-09 Advaxis Inc Compositions à base de listeria comprenant un système d'expression de minigènes codant pour des peptides, et leurs procédés d'utilisation
US10568516B2 (en) 2015-06-22 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
JP2018534921A (ja) * 2015-10-01 2018-11-29 マサチューセッツ インスティテュート オブ テクノロジー 生物学的ステートマシン
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
WO2017083750A1 (en) 2015-11-11 2017-05-18 Intrexon Corporation Compositions and methods for expression of multiple biologically active polypeptides from a single vector for treatment of cardiac conditions and other pathologies
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
KR20190082850A (ko) 2016-11-30 2019-07-10 어드박시스, 인크. 반복되는 암 돌연변이를 표적화하는 면역원 조성물 및 그것의 사용 방법
CA3049652A1 (en) * 2017-01-10 2018-07-19 Intrexon Corporation Modulating expression of polypeptides via new gene switch expression systems
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
EP3635132A4 (en) 2017-06-07 2021-05-26 Intrexon Corporation EXPRESSION OF NOVEL CELL MARKERS
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3749754A4 (en) 2018-02-08 2021-10-20 Applied Stemcell, Inc. TARGET GENE VARIANT SCREENING METHODS
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
CN111019873B (zh) * 2018-10-09 2022-08-19 安徽吐露港生物科技有限公司 在食气梭菌基因组上快速整合大片段dna的方法
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
AU2019370387A1 (en) * 2018-11-02 2021-05-27 Greenvenus, Llc Serine recombinases mediating stable integration into plant genomes
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
CN110093354B (zh) * 2019-04-17 2020-06-05 湖南杂交水稻研究中心 一种固定植物杂种优势的种子分选方法
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220259617A1 (en) 2019-06-13 2022-08-18 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
AU2020316034A1 (en) * 2019-07-23 2022-02-10 Janssen Biotech, Inc. Synthetic genetic elements for biomanufacture
JP7839728B2 (ja) * 2019-10-09 2026-04-02 ザ ジャクソン ラボラトリー 高頻度標的化動物遺伝子導入
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
EP4061940A4 (en) * 2019-11-22 2024-10-23 Flagship Pioneering Innovations VI, LLC RECOMBINASE COMPOSITIONS AND METHODS OF USE
WO2021158651A1 (en) * 2020-02-04 2021-08-12 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for controlling production of polypeptides in cells
CN113355345B (zh) * 2020-03-06 2023-05-23 深圳蓝晶生物科技有限公司 一种基因组整合外源序列的方法
WO2021207401A1 (en) * 2020-04-07 2021-10-14 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites
CA3177481A1 (en) * 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CN112458059B (zh) * 2020-11-25 2021-07-23 杭州景杰生物科技股份有限公司 一种识别H3 K18la兔单克隆抗体稳转细胞株及其构建方法
IL303269A (en) 2020-12-03 2023-07-01 Century Therapeutics Inc Genetically engineered cells and uses thereof
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
TW202241935A (zh) 2020-12-18 2022-11-01 美商世紀治療股份有限公司 具有可調適受體專一性之嵌合抗原受體系統
US20260055430A1 (en) * 2021-03-08 2026-02-26 Flagship Pioneering Innovations Vi, Llc Lentivirus with altered integrase activity
JP2024535618A (ja) 2021-10-14 2024-09-30 アシモフ，インコーポレーテッド インテグラーゼ、ランディングパッド構造、およびそれを含む操作された細胞
CN114107380B (zh) * 2021-11-05 2024-06-07 上海药明生物技术有限公司 一种CHO-S.attp重组细胞株及其构建方法和应用
WO2023114992A1 (en) * 2021-12-17 2023-06-22 Massachusetts Institute Of Technology Programmable insertion approaches via reverse transcriptase recruitment
CN117430696A (zh) * 2022-07-22 2024-01-23 北京智仁美博生物科技有限公司 用于表达Fab片段库的重组系统
US20240271161A1 (en) * 2022-09-16 2024-08-15 Editas Medicine, Inc. Novel recombinases and methods of use
WO2024102666A2 (en) * 2022-11-07 2024-05-16 Metagenomi, Inc. Serine recombinases for gene editing
WO2024192141A1 (en) 2023-03-13 2024-09-19 Dana-Farber Cancer Institute, Inc. Treatment of cancers having a drug-resistant mesenchymal cell state
WO2024197185A1 (en) 2023-03-21 2024-09-26 The Broad Institute, Inc. Methods and compositions for dissecting organelle physiology
WO2024226838A2 (en) 2023-04-25 2024-10-31 The Brigham And Women's Hospital, Inc. Treatment of autoimmune diseases having a pathogenic t cell state
KR20260044268A (ko) 2023-06-26 2026-04-01 유니버시티 오브 하와이 진화한 인테그라제 및 이를 이용한 게놈 편집 방법
CN117089574A (zh) * 2023-08-25 2023-11-21 上海科技大学 一种构建用于外源基因定点整合的果蝇品系的方法
WO2025049788A1 (en) 2023-08-29 2025-03-06 The Broad Institute, Inc. Optical genetic screens of intracellular and intercellular transcriptional circuits with perturb-fish
WO2025096916A1 (en) 2023-11-03 2025-05-08 The Broad Institute, Inc. Multi-site editing in living cells
WO2025129158A1 (en) 2023-12-15 2025-06-19 The Broad Institute, Inc. Engineered arc delivery vesicles and uses thereof
WO2025259766A1 (en) * 2024-06-11 2025-12-18 Komo Biosciences, Inc. Genome editing using evolved integrases
WO2026026962A1 (zh) * 2024-08-02 2026-02-05 北京齐禾生科生物科技有限公司 一种基于大丝氨酸重组酶的基因组编辑系统及其应用
CN119286893A (zh) * 2024-12-11 2025-01-10 三亚中国农业科学院国家南繁研究院 一种重组酶位点变体的筛选方法
CN119307470A (zh) * 2024-12-13 2025-01-14 三亚中国农业科学院国家南繁研究院 一种丝氨酸重组酶及其在植物中的应用

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5175385A (en) 1987-09-03 1992-12-29 Ohio University/Edison Animal Biotechnolgy Center Virus-resistant transgenic mice
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
DE69012601T2 (de) 1989-02-02 1995-01-19 Joel S Greenberger Gentherapie durch stützgewebebezügliche Zellen.
US5354844A (en) 1989-03-16 1994-10-11 Boehringer Ingelheim International Gmbh Protein-polycation conjugates
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5693622A (en) 1989-03-21 1997-12-02 Vical Incorporated Expression of exogenous polynucleotide sequences cardiac muscle of a mammal
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
US5750376A (en) 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
FR2714830B1 (fr) 1994-01-10 1996-03-22 Rhone Poulenc Rorer Sa Composition contenant des acides nucléiques, préparation et utilisations.
FR2715847B1 (fr) 1994-02-08 1996-04-12 Rhone Poulenc Rorer Sa Composition contenant des acides nucléiques, préparation et utilisations.
US5763198A (en) 1994-07-22 1998-06-09 Sugen, Inc. Screening assays for compounds
FR2727679B1 (fr) 1994-12-05 1997-01-03 Rhone Poulenc Rorer Sa Nouveaux agents de transfection et leurs applications pharmaceutiques
FR2730637B1 (fr) 1995-02-17 1997-03-28 Rhone Poulenc Rorer Sa Composition pharmaceutique contenant des acides nucleiques, et ses utilisations
US5919653A (en) 1996-08-20 1999-07-06 Allelix Neuroscience Inc. Nucleic acids encoding a human glycine transporter
US6114600A (en) 1997-10-31 2000-09-05 The United States Of America As Represented By The Secretary Of Agriculture Resolution of complex integration patterns to obtain single copy transgenes
WO2000011155A1 (en) * 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
WO2001061049A1 (en) 2000-02-18 2001-08-23 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
BR0112633A (pt) 2000-07-21 2003-09-16 Us Agriculture Método para substituir, translocar e empilhar dna em genomas eucarióticos
AU2002221829A1 (en) 2000-11-10 2002-05-21 Artemis Pharmaceuticals Gmbh Modified recombinase
JP4068297B2 (ja) 2000-11-21 2008-03-26 独立行政法人科学技術振興機構 Ｙ型六方晶系フェライト薄膜およびその製造方法
GB0210128D0 (en) 2002-05-02 2002-06-12 Chiron Spa Nucleic acids and proteins from streptococcus groups A & B
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
WO2005017170A2 (en) 2002-06-04 2005-02-24 Michele Calos Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
EP2801614B1 (en) * 2002-08-29 2019-05-15 The Board of Trustees of the Leland Stanford Junior University Circular nucleic acid vectors and methods for making and using the same
US7491539B2 (en) * 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
WO2004080162A2 (en) 2003-03-07 2004-09-23 Avigenics, Inc. Integrase mediated avian transgenesis
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
US20060046294A1 (en) 2004-08-26 2006-03-02 The United States Of America, As Represented By The Secretary Of Agriculture Site-specific recombination systems for use in eukaryotic cells
US9034650B2 (en) * 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
US8101169B2 (en) 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection

Also Published As

Publication number Publication date
IL211820A0 (en) 2011-05-31
AU2005326917A1 (en) 2006-08-10
CA2827515A1 (en) 2006-08-10
US20180346934A1 (en) 2018-12-06
MX2007009349A (es) 2008-03-07
JP6158639B2 (ja) 2017-07-05
EP1844155B1 (en) 2013-04-24
BRPI0519865A2 (pt) 2009-03-24
CN102994492B (zh) 2015-04-29
WO2006083253A1 (en) 2006-08-10
CA2914181A1 (en) 2006-08-10
CN101194018B (zh) 2012-11-28
US20170114370A1 (en) 2017-04-27
EP2327786B1 (en) 2016-04-27
IL185027A (en) 2014-04-30
ES2584911T3 (es) 2016-09-30
EP1844155A4 (en) 2008-03-12
DK1844155T3 (da) 2013-07-29
JP2017136080A (ja) 2017-08-10
CA2595929A1 (en) 2006-08-10
US10081817B2 (en) 2018-09-25
CA3048118A1 (en) 2006-08-10
AU2005326917B2 (en) 2012-04-19
JP6577969B2 (ja) 2019-09-18
JP5684445B2 (ja) 2015-03-11
HK1107119A1 (en) 2008-03-28
JP2014000085A (ja) 2014-01-09
KR20080003780A (ko) 2008-01-08
IL211819A0 (en) 2011-05-31
JP5796975B2 (ja) 2015-10-21
NZ556716A (en) 2011-02-25
EP2327786A3 (en) 2012-03-21
CN101194018A (zh) 2008-06-04
JP2011167195A (ja) 2011-09-01
CA2595929C (en) 2016-02-02
US20080020465A1 (en) 2008-01-24
NZ583562A (en) 2012-04-27
EP1844155A1 (en) 2007-10-17
EP3112457A1 (en) 2017-01-04
IL211820A (en) 2015-01-29
US9034650B2 (en) 2015-05-19
JP2008528056A (ja) 2008-07-31
US20060172377A1 (en) 2006-08-03
CN102994492A (zh) 2013-03-27
DK2327786T3 (en) 2016-08-15
EP2327786A2 (en) 2011-06-01
CA2914181C (en) 2019-08-06
US20150275232A1 (en) 2015-10-01
SG10201509276YA (en) 2015-12-30
IL211819A (en) 2015-01-29
IL185027A0 (en) 2007-12-03
US10648003B2 (en) 2020-05-12

Similar Documents

Publication Publication Date Title
CY1120743T1 (el) Μεθοδος επιθεωρησης εσωτερικου χωρου ενος πλοιου και/η εκτελεσης εργασιων εντος αυτου
MX2021013857A (es) Biomateriales compuestos.
WO2018136562A3 (en) Compositions and methods for immune repertoire sequencing
BRPI0519865A2 (pt) serina recombinases sìtio-especìficas e métodos para seu uso
GB2432845A (en) Gradient scaffolding and methods of producing the same
HN2008001104A (es) Compuestos para anticuerpos de dickpf-1 y70-4
AU2017257225A1 (en) Allele editing and applications thereof
AR080009A1 (es) Alteracion genomica objetivo utilizando nucleasas de dedos de zinc
IN2012DN02452A (es)
CL2007003161A1 (es) Anticuerpos anti-factor d; acidos nucleicos que los codifican; vector y linea celular que los comprenden; metodo de produccion; composicion farmaceutica que comprende a los anticuerpos; y su uso para tratar trastornos mediados por el complemento como
EP3805383A4 (en) ANTIFIBROSIS COMPOSITION, CELLS PRODUCING THESE CELLS AND CELL LAYER CONTAINING THESE CELLS
CL2017001903A1 (es) Composición novedosa para uso en el incremento de eficacia de injerto de células madre hematopoyéticas después del trasplante
UY38519A (es) Composiciones y procedimientos para la detección de ácido nucleico de especies de plasmodium
MX2025012083A (es) Imprimacion híbrida poliuretano-silano a base de agua, métodos para su preparación y métodos de uso
WO2015051122A3 (en) Wnt compositions and methods for purification
WO2005109555A3 (en) System for and method of performing electrochemical tests of solid oxide fuel cells
EP4297118A4 (en) ELECTRODE ASSEMBLY, BATTERY ELEMENT, BATTERY, AND METHOD AND DEVICE FOR MANUFACTURING ELECTRODE ASSEMBLY
BR112021019657A2 (pt) Método para produzir célula knock-in
WO2019112760A3 (en) Structures and components having composite unit cell matrix construction
EP4369437A4 (en) ANODE COMPOSITION, ANODE INCLUDING THE ANODE FOR SECONDARY LITHIUM BATTERY, SECONDARY LITHIUM BATTERY INCLUDING THE ANODE, AND METHOD FOR PREPARING ANODE COMPOSITION
MX2024003986A (es) Metodos para cuantificar el impacto de la tension de cizallamiento en lineas de celulas cho.
EP4386914A4 (en) ELECTROCHEMICAL CELL, METHOD FOR GENERATING POWER USING AN ELECTROCHEMICAL CELL, AND METHOD FOR GENERATING HYDROGEN GAS USING AN ELECTROCHEMICAL CELL
GT200900134A (es) Sistema y mètodo para mejorar las comunicaciones entres vehiculos
ATE516562T1 (de) Verfahren und system zur bildsynthese
TW200726827A (en) In situ fluoride ion-generating compositions and uses thereof