ES2426031T3 - Métodos mejorados para BEAMing - Google Patents

Métodos mejorados para BEAMing Download PDF

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Publication number
ES2426031T3
ES2426031T3 ES11191989T ES11191989T ES2426031T3 ES 2426031 T3 ES2426031 T3 ES 2426031T3 ES 11191989 T ES11191989 T ES 11191989T ES 11191989 T ES11191989 T ES 11191989T ES 2426031 T3 ES2426031 T3 ES 2426031T3
Authority
ES
Spain
Prior art keywords
dna
beads
amplicons
homo sapiens
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES11191989T
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English (en)
Spanish (es)
Inventor
Bert Vogelstein
Kenneth Kinzler
Frank Diehl
Ming Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Application granted granted Critical
Publication of ES2426031T3 publication Critical patent/ES2426031T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R31/00—Arrangements for testing electric properties; Arrangements for locating electric faults; Arrangements for electrical testing characterised by what is being tested not provided for elsewhere
    • G01R31/327—Testing of circuit interrupters, switches or circuit-breakers
    • G01R31/3277—Testing of circuit interrupters, switches or circuit-breakers of low voltage devices, e.g. domestic or industrial devices, such as motor protections, relays, rotation switches
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R31/00—Arrangements for testing electric properties; Arrangements for locating electric faults; Arrangements for electrical testing characterised by what is being tested not provided for elsewhere
    • G01R31/50—Testing of electric apparatus, lines, cables or components for short-circuits, continuity, leakage current or incorrect line connections
    • G01R31/52—Testing for short-circuits, leakage current or ground faults
    • H—ELECTRICITY
    • H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02H—EMERGENCY PROTECTIVE CIRCUIT ARRANGEMENTS
    • H02H1/00—Details of emergency protective circuit arrangements
    • H02H1/0007—Details of emergency protective circuit arrangements concerning the detecting means
    • H02H1/0015—Using arc detectors
    • H—ELECTRICITY
    • H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02H—EMERGENCY PROTECTIVE CIRCUIT ARRANGEMENTS
    • H02H3/00—Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection
    • H02H3/26—Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents
    • H02H3/32—Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents involving comparison of the voltage or current values at corresponding points in different conductors of a single system, e.g. of currents in go and return conductors
    • H02H3/33—Emergency protective circuit arrangements for automatic disconnection directly responsive to an undesired change from normal electric working condition with or without subsequent reconnection ; integrated protection responsive to difference between voltages or between currents; responsive to phase angle between voltages or between currents involving comparison of the voltage or current values at corresponding points in different conductors of a single system, e.g. of currents in go and return conductors using summation current transformers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Power Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Vascular Medicine (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nanotechnology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ES11191989T 2005-10-24 2006-10-20 Métodos mejorados para BEAMing Active ES2426031T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72923505P 2005-10-24 2005-10-24
US729235P 2005-10-24

Publications (1)

Publication Number Publication Date
ES2426031T3 true ES2426031T3 (es) 2013-10-18

Family

ID=37968413

Family Applications (2)

Application Number Title Priority Date Filing Date
ES11191989T Active ES2426031T3 (es) 2005-10-24 2006-10-20 Métodos mejorados para BEAMing
ES06817236T Active ES2381204T3 (es) 2005-10-24 2006-10-20 Métodos mejorados para BEAMING

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES06817236T Active ES2381204T3 (es) 2005-10-24 2006-10-20 Métodos mejorados para BEAMING

Country Status (10)

Country Link
US (4) US9360526B2 (de)
EP (2) EP1948816B1 (de)
AT (1) ATE536420T1 (de)
CY (1) CY1112525T1 (de)
DK (1) DK1948816T3 (de)
ES (2) ES2426031T3 (de)
PL (1) PL1948816T3 (de)
PT (1) PT1948816E (de)
SI (1) SI1948816T1 (de)
WO (1) WO2007050465A2 (de)

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US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
DE602005009324D1 (de) 2005-04-06 2008-10-09 Maurice Stroun Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf
EP1948816B1 (de) * 2005-10-24 2011-12-07 The Johns Hopkins University Verbesserte verfahren für beaming
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
GB2453173A (en) * 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US20100041048A1 (en) * 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
EP3216874A1 (de) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP4435111A1 (de) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013083810A1 (en) * 2011-12-09 2013-06-13 F. Hoffmann-La Roche Ag Identification of non-responders to her2 inhibitors
US20130309667A1 (en) * 2012-05-15 2013-11-21 Anthony P. Shuber Primers for analyzing methylated sequences and methods of use thereof
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
DE102013211113A1 (de) * 2013-06-14 2014-12-18 Siemens Aktiengesellschaft Verfahren zur kombinierten Quantifizierung und Sequenzierung von mindestens einer Ziel-Nukleinsäure
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
WO2016152236A1 (ja) * 2015-03-24 2016-09-29 日立金属株式会社 セラミックハニカム構造体
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
US11220705B2 (en) * 2015-04-24 2022-01-11 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
US9938572B1 (en) 2015-09-08 2018-04-10 Raindance Technologies, Inc. System and method for forming an emulsion
CN106929598B (zh) * 2017-05-04 2019-07-05 湖南融健基因生物科技有限公司 一种乳液中进行滚环扩增的数字核酸分析方法
CN111565731B (zh) * 2017-10-24 2024-03-08 达莉亚·伊兰妮 治疗缺血性疾病的方法
US11168373B2 (en) 2018-09-17 2021-11-09 The University Of North Carolina At Chapel Hill Method for quantifying DNA fragments in a sample by size
US11474109B2 (en) 2018-11-16 2022-10-18 Scintimetrics, Inc. Compositions and methods for controllably merging emulsion droplets and sample analysis
EP3725894A1 (de) 2019-04-16 2020-10-21 Blod Diagnostik GmbH Vorrichtung und verfahren zur nukleinsäurezielanreicherung, -suspension und -quantifizierung
CN114902024B (zh) * 2019-11-15 2026-05-26 闪璨迈璀公司 基于荧光团扩散的组合物和方法

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US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DE10048797A1 (de) * 2000-10-02 2002-04-18 Bayer Ag Wirkstoffhaltige Emulsionen
AUPS298102A0 (en) * 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
DE602004026033D1 (de) * 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US8048627B2 (en) * 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
WO2005073410A2 (en) * 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
WO2006076022A2 (en) * 2004-05-13 2006-07-20 Anita Goel Nano-pcr: methods and devices for nucleic acid amplification and detection
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
EP1948816B1 (de) 2005-10-24 2011-12-07 The Johns Hopkins University Verbesserte verfahren für beaming
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays

Also Published As

Publication number Publication date
ATE536420T1 (de) 2011-12-15
EP2428579A1 (de) 2012-03-14
US20120014879A1 (en) 2012-01-19
US20200123600A1 (en) 2020-04-23
PL1948816T3 (pl) 2012-07-31
US10150991B2 (en) 2018-12-11
EP1948816B1 (de) 2011-12-07
US10837050B2 (en) 2020-11-17
EP1948816A2 (de) 2008-07-30
ES2381204T3 (es) 2012-05-24
PT1948816E (pt) 2012-03-19
US20110059435A1 (en) 2011-03-10
US9360526B2 (en) 2016-06-07
DK1948816T3 (da) 2012-04-02
SI1948816T1 (sl) 2012-04-30
US20160376645A1 (en) 2016-12-29
WO2007050465A3 (en) 2007-10-25
CY1112525T1 (el) 2015-12-09
EP2428579B1 (de) 2013-05-29
EP1948816A4 (de) 2009-11-04
US20130040300A9 (en) 2013-02-14
WO2007050465A2 (en) 2007-05-03
HK1121193A1 (en) 2009-04-17

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