ES2488121T3 - Procedimiento para la obtención de plantas transgénicas resistentes a los fitopatógenos basado en la interferencia por ARN (ARNi) - Google Patents

Procedimiento para la obtención de plantas transgénicas resistentes a los fitopatógenos basado en la interferencia por ARN (ARNi) Download PDF

Info

Publication number
ES2488121T3
ES2488121T3 ES09797438.0T ES09797438T ES2488121T3 ES 2488121 T3 ES2488121 T3 ES 2488121T3 ES 09797438 T ES09797438 T ES 09797438T ES 2488121 T3 ES2488121 T3 ES 2488121T3
Authority
ES
Spain
Prior art keywords
seq
receptor
rapg
nucleotide sequence
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09797438.0T
Other languages
English (en)
Inventor
Stefania Arciello
Davide Andrenacci
Fabio Apone
Maria Gabriella Colucci
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arterra Bioscience SpA
Original Assignee
Arterra Bioscience SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arterra Bioscience SpA filed Critical Arterra Bioscience SpA
Application granted granted Critical
Publication of ES2488121T3 publication Critical patent/ES2488121T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Procedimiento para la preparación de plantas transgénicas resistentes al ataque de fitopatógenos como Spodoptera littoralis y/o Meloidogyne incognita por interferencia del ARN que comprende las etapas siguientes: a) aislar la secuencia de nucleótidos de ADNc que codifica un receptor acoplado a la proteína G (RAPG), o una porción del mismo, cuya función es vital para el fitopatógeno, en el que dicha secuencia de nucleótidos del ADNc codifica un receptor RAPG seleccionado de entre AlstCR de SEC ID nº 2, DHR de SEC ID nº 3 u Oct/TyrR de SEC ID nº 6 cuando dicho insecto es Spodoptera littoralis y en el que dicha secuencia de nucleótidos de ADNc codifica un receptor RAPG seleccionado de entre el receptor de tipo serotonina de SEC ID nº 9 o el receptor de neuropéptidos de SEC ID nº 11 cuando dicho fitopatógeno es Meloidogyne incognita; b) construir un vector de expresión que comprende la secuencia de nucleótidos de ADNc que codifica un receptor RAPG o una porción del mismo según se determina en la etapa a), flanqueado por dos sitios de recombinación específicos; c) reaccionar por recombinación el vector de expresión de la etapa b) con un vector binario que comprende un activador constitutivo o específico para el tejido, los mismos sitios de recombinación específicos y una secuencia intrónica para obtener un plásmido recombinante; d) transferir dicho plásmido recombinante en células competentes de Agrobacterium tumefaciens y transformar la planta; e) cultivar la planta y obtener la expresión constitutiva o específica para el tejido de ARNbc en dicha planta.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15

Claims (1)

  1. imagen1
ES09797438.0T 2008-07-17 2009-07-15 Procedimiento para la obtención de plantas transgénicas resistentes a los fitopatógenos basado en la interferencia por ARN (ARNi) Active ES2488121T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITMI2008A001305A IT1393648B1 (it) 2008-07-17 2008-07-17 Metodo per l'ottenimento di piante transgeniche resistenti all'attacco di fitopatogeni basato sull'interferenza dell'rna (rnai)
ITMI20081305 2008-07-17
PCT/EP2009/005203 WO2010006804A1 (en) 2008-07-17 2009-07-15 Method to obtain transgenic plants resistant to phytopathogen attack based on rna interference (rnai)

Publications (1)

Publication Number Publication Date
ES2488121T3 true ES2488121T3 (es) 2014-08-26

Family

ID=40532516

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09797438.0T Active ES2488121T3 (es) 2008-07-17 2009-07-15 Procedimiento para la obtención de plantas transgénicas resistentes a los fitopatógenos basado en la interferencia por ARN (ARNi)

Country Status (7)

Country Link
US (1) US20110150839A1 (es)
EP (1) EP2310515B1 (es)
CN (1) CN102124114A (es)
BR (1) BRPI0916445A2 (es)
ES (1) ES2488121T3 (es)
IT (1) IT1393648B1 (es)
WO (1) WO2010006804A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9587248B2 (en) 2011-01-12 2017-03-07 The United States Of America As Represented By The Secretary Of Agriculture Mayetiola destructor susceptibility gene one (Mds-1) and its application in pest management
CN102399787B (zh) * 2011-10-31 2013-03-20 东北农业大学 大豆食心虫核糖体蛋白基因及其应用
CN103665131B (zh) * 2012-09-12 2015-07-29 中国农业大学 棉铃虫和烟夜蛾的npf神经肽及其编码基因和应用
CN103665132B (zh) * 2012-09-12 2015-08-26 中国农业大学 棉铃虫和烟夜蛾的npy神经肽及其编码基因和应用
US10308954B2 (en) 2012-12-14 2019-06-04 Icar Method for the control of nematodes in plants
EP3140401A2 (en) 2014-05-04 2017-03-15 Forrest Innovations Ltd. Compositions for mosquito control and uses of same
CN111733171B (zh) * 2020-06-23 2022-02-25 中国农业科学院郑州果树研究所 抗根癌病的靶基因片段及其干扰载体和应用
CN115838742B (zh) * 2022-10-18 2024-06-21 华中农业大学 南方根结线虫去甲基化酶Mi-NMAD-1/2基因及其应用
CN116622714B (zh) * 2023-02-02 2023-11-03 广东省农业科学院植物保护研究所 褐飞虱NlA7基因及其dsRNA在防治褐飞虱的应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5228926A (en) * 1990-09-20 1993-07-20 United Solar Systems Corporation Photovoltaic device with increased light absorption and method for its manufacture
DE4130092A1 (de) * 1991-03-28 1992-10-01 Nipox K K Fresnel'sche linse
US6060594A (en) * 1997-12-18 2000-05-09 Ecogen, Inc. Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US6733872B2 (en) * 2001-03-01 2004-05-11 Asahi Glass Company, Limited Laminated glass
JP4124991B2 (ja) * 2001-10-23 2008-07-23 Ｄｉｃ株式会社 フレネルレンズ用活性エネルギー線硬化型樹脂組成物及びフレネルレンズシート
JP2005512539A (ja) * 2001-12-18 2005-05-12 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 昆虫ｇタンパク質共役型受容体遺伝子及びそれらの使用
US6885371B2 (en) * 2002-04-30 2005-04-26 Hewlett-Packard Development Company, L.P. System and method of identifying a selected image object in a three-dimensional graphical environment
WO2004038462A1 (en) * 2002-10-22 2004-05-06 Sunray Technologies, Inc. Diffractive structures for the redirection and concentration of optical radiation
EP1807522B1 (en) * 2004-10-21 2013-11-20 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
WO2006101854A2 (en) * 2005-03-16 2006-09-28 Kansas State University Research Foundation Compositions and methods for controlling fungal diseases
DE102005017170B4 (de) * 2005-04-13 2010-07-01 Ovd Kinegram Ag Transferfolie, Verfahren zu deren Herstellung sowie Mehrschichtkörper und dessen Verwendung
DE102005033272A1 (de) * 2005-06-03 2006-12-07 Solartec Ag Konzentrator-Photovoltaik-Einrichtung, daraus gebildetes PV-Konzentratormodul sowie Herstellverfahren hierfür
CN101195821A (zh) * 2006-12-04 2008-06-11 中国科学院上海生命科学研究院 利用RNAi技术改良植物抗虫性的方法
CN101646769A (zh) * 2007-02-20 2010-02-10 孟山都技术公司 无脊椎动物微rna

Also Published As

Publication number Publication date
EP2310515A1 (en) 2011-04-20
CN102124114A (zh) 2011-07-13
IT1393648B1 (it) 2012-05-08
WO2010006804A1 (en) 2010-01-21
EP2310515B1 (en) 2014-04-16
BRPI0916445A2 (pt) 2018-10-16
WO2010006804A8 (en) 2010-04-29
US20110150839A1 (en) 2011-06-23
ITMI20081305A1 (it) 2010-01-18

Similar Documents

Publication Publication Date Title
AR054171A1 (es) Aumento de rendimiento en plantas con sobreexpresion de los genes accdp
AR091299A1 (es) Polinucleotidos y polipeptidos aislados y metodos para utilizarlos para mejorar el rendimiento de las plantas
MX377436B (es) Polinucleotidos y polipeptidos aislados, y metodos para usarlos para incrementar el rendimiento de una planta y/o caracteristicas agricolas.
MX393132B (es) Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para aumentar la eficacia en el uso de nitrogeno, rendimiento, tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
AR069893A1 (es) Plantas con mayor rendimiento y/o mayor tolerancia al estres ambiental (iy-bm)
AR114027A2 (es) Construcciones de adn recombinante y métodos para modular la expresión de un gen diana
ES2383716T3 (es) Composiciones y métodos que utilizan ARN de interferencia de un gen del tipo OPR3 para el control de nemátodos
AR059433A1 (es) Identificacion y uso de genes blanco para el control de nematodos parasitos de plantas
AR088595A1 (es) Una planta con tolerancia a la sequia, eficiencia en el uso de nitrogeno y rendimiento mejorados
AR060435A1 (es) Polipeptidos relacionados con esteres y metodos para su uso en plantas
MX393710B (es) Polinucleotidos y polipeptidos aislados, sus metodos de utilizacion para mejorar la eficacia en el uso del nitrogeno, rendimiento tasa de crecimiento, vigor, biomasa, contenido de aceite y/o tolerancia al estres abiotico.
ES2531135T3 (es) Fitasas, ácidos nucleicos que las codifican y métodos para su producción y uso
MX395168B (es) Polinucleotidos y polipeptidos aislados, y metodos para utilizarlos para mejorar el rendimiento de la planta y/o las caracteristicas agricolas.
AR070693A1 (es) Plantas que tienen rasgos aumentados relacionados con el rendimiento y un metodo para obtenerlas a partir de la expresion del polipeptido de nitrato 2 (nrt2)
CL2012000799A1 (es) Proteína cryba que tiene una solubilidad o actividad insecticida mejorada contra al menos el gusano barrenador europeo (ostrinia nubilalis); molecula de acido nucleico que la codifica; gen quimérico, vector y celula huesped de dicho acido nucleico; método de producción; composicion insecticida; metodo para producir una planta resistente a insectos; y metodo de control de un insecto lepidóptero.
BR112014004130A8 (pt) Polinucleotídeos e polipeptídeos isolados e métodos de uso dos mesmos para o aumento da prooução da planta e/ou características agrícolas
AR069821A1 (es) Composiciones y metodos de uso de la interferencia de arn para el control de nematodos
AR065242A1 (es) Promotores y elementos reguladores de genes vegetales de tipo mtn3 inducibles por nematodos
CU23936B1 (es) Plásmido para la expresión sexo-específica en un insecto de empalmes alternativos
AR074589A1 (es) Planta con resistencia especifica de raiz a nematodos
AR084844A1 (es) Arboles de citricos con resistencia a cancro de los citricos
Luke et al. Growing uses of 2A in plant biotechnology
Dong et al. Densovirus infection facilitates plant–virus transmission by an aphid.
AR114287A2 (es) Constructo de un gen quimérico aislado; polipéptido codificado por dicho constructo; y método para conferir a una planta tolerancia a sequía, alta salinidad y congelamiento, excluida la planta obtenida por dicho método
AR069770A1 (es) Casette de expresion y metodos para aumentar los rendimientos de una planta