ES2502841T3 - Métodos y medios para la proliferación de células madres y generación y expansión posterior de células progenitoras, así como producción de células efectoras como terapéuticos clínicos - Google Patents
Métodos y medios para la proliferación de células madres y generación y expansión posterior de células progenitoras, así como producción de células efectoras como terapéuticos clínicos Download PDFInfo
- Publication number
- ES2502841T3 ES2502841T3 ES06799473.1T ES06799473T ES2502841T3 ES 2502841 T3 ES2502841 T3 ES 2502841T3 ES 06799473 T ES06799473 T ES 06799473T ES 2502841 T3 ES2502841 T3 ES 2502841T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- stem cells
- differentiation
- medium
- differentiating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 131
- 210000004027 cell Anatomy 0.000 title claims description 104
- 238000000034 method Methods 0.000 title claims description 32
- 239000003814 drug Substances 0.000 title description 4
- 239000012636 effector Substances 0.000 title description 4
- 230000004663 cell proliferation Effects 0.000 title description 2
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000002609 medium Substances 0.000 claims abstract description 92
- 230000004069 differentiation Effects 0.000 claims abstract description 55
- 102000004127 Cytokines Human genes 0.000 claims abstract description 28
- 108090000695 Cytokines Proteins 0.000 claims abstract description 28
- 210000002966 serum Anatomy 0.000 claims abstract description 28
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims abstract description 20
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims abstract description 20
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims abstract description 20
- 108090001005 Interleukin-6 Proteins 0.000 claims abstract description 19
- RDHQFKQIGNGIED-MRVPVSSYSA-N O-acetyl-L-carnitine Chemical compound CC(=O)O[C@H](CC([O-])=O)C[N+](C)(C)C RDHQFKQIGNGIED-MRVPVSSYSA-N 0.000 claims abstract description 19
- 102000004889 Interleukin-6 Human genes 0.000 claims abstract description 17
- 229960002897 heparin Drugs 0.000 claims abstract description 17
- 229920000669 heparin Polymers 0.000 claims abstract description 17
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims abstract description 16
- -1 stem cell factor Proteins 0.000 claims abstract description 15
- 101100335081 Mus musculus Flt3 gene Proteins 0.000 claims abstract description 12
- 239000003446 ligand Substances 0.000 claims abstract description 12
- 102000036693 Thrombopoietin Human genes 0.000 claims abstract description 11
- 108010041111 Thrombopoietin Proteins 0.000 claims abstract description 11
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 claims abstract description 8
- 239000006143 cell culture medium Substances 0.000 claims abstract description 5
- 230000002792 vascular Effects 0.000 claims description 17
- 102100032352 Leukemia inhibitory factor Human genes 0.000 claims description 14
- 108090000581 Leukemia inhibitory factor Proteins 0.000 claims description 14
- 102100034195 Thrombopoietin Human genes 0.000 claims description 13
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims description 13
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims description 13
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 12
- 108010002586 Interleukin-7 Proteins 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 10
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 9
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 9
- 102100032528 C-type lectin domain family 11 member A Human genes 0.000 claims description 8
- 108010002350 Interleukin-2 Proteins 0.000 claims description 8
- 102000000588 Interleukin-2 Human genes 0.000 claims description 8
- 102100020880 Kit ligand Human genes 0.000 claims description 8
- 102100037852 Insulin-like growth factor I Human genes 0.000 claims description 7
- 101000942297 Homo sapiens C-type lectin domain family 11 member A Proteins 0.000 claims description 6
- 101710113649 Thyroid peroxidase Proteins 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 102000000646 Interleukin-3 Human genes 0.000 claims description 5
- 108010002386 Interleukin-3 Proteins 0.000 claims description 5
- 239000007640 basal medium Substances 0.000 claims description 5
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 claims description 4
- 102000003812 Interleukin-15 Human genes 0.000 claims description 4
- 108090000172 Interleukin-15 Proteins 0.000 claims description 4
- 101710177504 Kit ligand Proteins 0.000 claims description 4
- 101001055320 Myxine glutinosa Insulin-like growth factor Proteins 0.000 claims description 4
- 210000000773 pre-NK cell Anatomy 0.000 claims description 4
- 102000009088 Angiopoietin-1 Human genes 0.000 claims description 3
- 108010048154 Angiopoietin-1 Proteins 0.000 claims description 3
- 102000009075 Angiopoietin-2 Human genes 0.000 claims description 3
- 108010048036 Angiopoietin-2 Proteins 0.000 claims description 3
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 3
- FSPQCTGGIANIJZ-UHFFFAOYSA-N 2-[[(3,4-dimethoxyphenyl)-oxomethyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxamide Chemical compound C1=C(OC)C(OC)=CC=C1C(=O)NC1=C(C(N)=O)C(CCCC2)=C2S1 FSPQCTGGIANIJZ-UHFFFAOYSA-N 0.000 claims description 2
- 102100020718 Receptor-type tyrosine-protein kinase FLT3 Human genes 0.000 claims description 2
- 101710151245 Receptor-type tyrosine-protein kinase FLT3 Proteins 0.000 claims description 2
- 102000000704 Interleukin-7 Human genes 0.000 claims 2
- 102100023487 Lens fiber major intrinsic protein Human genes 0.000 abstract 1
- 108010047660 Mitochondrial intermediate peptidase Proteins 0.000 abstract 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 16
- 210000004700 fetal blood Anatomy 0.000 description 14
- 210000000822 natural killer cell Anatomy 0.000 description 13
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 12
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 12
- 102100021592 Interleukin-7 Human genes 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 10
- 229960005070 ascorbic acid Drugs 0.000 description 8
- 235000010323 ascorbic acid Nutrition 0.000 description 8
- 239000011668 ascorbic acid Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000013589 supplement Substances 0.000 description 8
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 7
- 229930182816 L-glutamine Natural products 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 229960002743 glutamine Drugs 0.000 description 7
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 6
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 6
- XYUNNDAEUQFHGV-UHFFFAOYSA-N [Se].[Se] Chemical compound [Se].[Se] XYUNNDAEUQFHGV-UHFFFAOYSA-N 0.000 description 6
- 208000032839 leukemia Diseases 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 101000577064 Lymnaea stagnalis Molluscan insulin-related peptide 1 Proteins 0.000 description 5
- 101000737895 Mytilus edulis Contraction-inhibiting peptide 1 Proteins 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 229930182555 Penicillin Natural products 0.000 description 5
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 229940049954 penicillin Drugs 0.000 description 5
- 238000010561 standard procedure Methods 0.000 description 5
- 229960005322 streptomycin Drugs 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 210000003954 umbilical cord Anatomy 0.000 description 5
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 4
- 102000009571 Macrophage Inflammatory Proteins Human genes 0.000 description 4
- 108010009474 Macrophage Inflammatory Proteins Proteins 0.000 description 4
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 230000003511 endothelial effect Effects 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 238000001802 infusion Methods 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 229940100601 interleukin-6 Drugs 0.000 description 4
- ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 4
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 3
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 3
- 102000014429 Insulin-like growth factor Human genes 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 3
- 108700025316 aldesleukin Proteins 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 229940100994 interleukin-7 Drugs 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 210000001939 mature NK cell Anatomy 0.000 description 3
- 230000009984 peri-natal effect Effects 0.000 description 3
- 239000008194 pharmaceutical composition Substances 0.000 description 3
- 210000001778 pluripotent stem cell Anatomy 0.000 description 3
- 229940087463 proleukin Drugs 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000003014 totipotent stem cell Anatomy 0.000 description 3
- 230000006444 vascular growth Effects 0.000 description 3
- LOGFVTREOLYCPF-KXNHARMFSA-N (2s,3r)-2-[[(2r)-1-[(2s)-2,6-diaminohexanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxybutanoic acid Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H]1CCCN1C(=O)[C@@H](N)CCCCN LOGFVTREOLYCPF-KXNHARMFSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101710167766 C-type lectin domain family 11 member A Proteins 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 241001227713 Chiron Species 0.000 description 2
- 102100020715 Fms-related tyrosine kinase 3 ligand protein Human genes 0.000 description 2
- 101710162577 Fms-related tyrosine kinase 3 ligand protein Proteins 0.000 description 2
- 101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 description 2
- 101000917839 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-B Proteins 0.000 description 2
- 101000971513 Homo sapiens Natural killer cells antigen CD94 Proteins 0.000 description 2
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 description 2
- 102000000589 Interleukin-1 Human genes 0.000 description 2
- 108010002352 Interleukin-1 Proteins 0.000 description 2
- 102000003777 Interleukin-1 beta Human genes 0.000 description 2
- 108090000193 Interleukin-1 beta Proteins 0.000 description 2
- 102100029185 Low affinity immunoglobulin gamma Fc region receptor III-B Human genes 0.000 description 2
- 102100021462 Natural killer cells antigen CD94 Human genes 0.000 description 2
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000002308 embryonic cell Anatomy 0.000 description 2
- 210000001671 embryonic stem cell Anatomy 0.000 description 2
- 210000003038 endothelium Anatomy 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
- 230000006303 immediate early viral mRNA transcription Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 210000002894 multi-fate stem cell Anatomy 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000275 quality assurance Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 208000003174 Brain Neoplasms Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 102100037362 Fibronectin Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 208000009329 Graft vs Host Disease Diseases 0.000 description 1
- 101000589305 Homo sapiens Natural cytotoxicity triggering receptor 2 Proteins 0.000 description 1
- 102000002698 KIR Receptors Human genes 0.000 description 1
- 108010043610 KIR Receptors Proteins 0.000 description 1
- 101150069255 KLRC1 gene Proteins 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 101100404845 Macaca mulatta NKG2A gene Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 102100022682 NKG2-A/NKG2-B type II integral membrane protein Human genes 0.000 description 1
- 108010004217 Natural Cytotoxicity Triggering Receptor 1 Proteins 0.000 description 1
- 108010004222 Natural Cytotoxicity Triggering Receptor 3 Proteins 0.000 description 1
- 102100032870 Natural cytotoxicity triggering receptor 1 Human genes 0.000 description 1
- 102100032851 Natural cytotoxicity triggering receptor 2 Human genes 0.000 description 1
- 102100032852 Natural cytotoxicity triggering receptor 3 Human genes 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 1
- 238000011316 allogeneic transplantation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000007792 gaseous phase Substances 0.000 description 1
- 208000024908 graft versus host disease Diseases 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940076264 interleukin-3 Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000007758 minimum essential medium Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000003169 placental effect Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000000250 revascularization Effects 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 210000001988 somatic stem cell Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
- C12N5/0692—Stem cells; Progenitor cells; Precursor cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/145—Thrombopoietin [TPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/165—Vascular endothelial growth factor [VEGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2306—Interleukin-6 (IL-6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Vascular Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05077221 | 2005-09-28 | ||
| EP05077221 | 2005-09-28 | ||
| PCT/NL2006/000484 WO2007037682A1 (fr) | 2005-09-28 | 2006-09-28 | Procédés et moyens pour la prolifération de cellules souches, pour la génération et le développement ultérieurs de cellules progénitrices et pour la production de cellules effectrices en tant que traitement clinique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2502841T3 true ES2502841T3 (es) | 2014-10-06 |
Family
ID=37307258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06799473.1T Active ES2502841T3 (es) | 2005-09-28 | 2006-09-28 | Métodos y medios para la proliferación de células madres y generación y expansión posterior de células progenitoras, así como producción de células efectoras como terapéuticos clínicos |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9109202B2 (fr) |
| EP (1) | EP1941027B1 (fr) |
| DK (1) | DK1941027T3 (fr) |
| ES (1) | ES2502841T3 (fr) |
| PL (1) | PL1941027T3 (fr) |
| PT (1) | PT1941027E (fr) |
| SI (1) | SI1941027T1 (fr) |
| WO (1) | WO2007037682A1 (fr) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2377923A1 (fr) | 2006-04-14 | 2011-10-19 | Advanced Cell Technology, Inc. | Cellules formant colonie d'hemangio |
| PL2142642T3 (pl) | 2007-03-27 | 2017-10-31 | Ipd Therapeutics B V | Sposoby i środki do proliferacji komórek macierzystych oraz następczego wytwarzania i ekspansji komórek progenitorowych, jak również wytwarzania komórek efektorowych jako leków klinicznych |
| PT2203176E (pt) | 2007-09-28 | 2015-03-02 | Anthrogenesis Corp | Supressão de tumor utilizando perfusato placentário humano e células assassinas naturais intermédias derivadas de placenta humanas |
| US20120003738A1 (en) * | 2007-10-04 | 2012-01-05 | University Hospitals Of Cleveland | Method For Amplification And Functional Enhancment Of Blood Derived Progenitor Cells Using A Closed Culture System |
| RU2359030C1 (ru) * | 2008-03-19 | 2009-06-20 | Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" | Способ получения эндотелиальных клеток из эмбриональных стволовых клеток человека (варианты) |
| ES2324011B1 (es) * | 2009-01-02 | 2010-03-15 | Banc De Sang I Teixits | Procedimiento para la obtencion de celulas madre mesenquimales a partir de la fraccion mononuclear de la medula osea humana. |
| AU2010326027A1 (en) * | 2009-12-04 | 2012-06-21 | Stem Cell & Regenerative Medicine International, Inc. | Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts |
| KR20220053695A (ko) | 2010-07-13 | 2022-04-29 | 셀룰래리티 인코포레이티드 | 천연 킬러 세포의 생성 방법 |
| ES2856825T3 (es) * | 2011-03-18 | 2021-09-28 | Glycostem Therapeutics B V | Generación de células NK y progenitores de células NK |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| CN104136034B (zh) | 2011-11-30 | 2018-04-24 | 安斯泰来再生医药协会 | 间充质基质细胞及其相关用途 |
| WO2013119118A1 (fr) * | 2012-02-08 | 2013-08-15 | Ipd-Therapeutics B.V. | Différenciation ex vivo de cellules tueuses naturelles à partir de cellules hématopoïétiques cd34+ |
| CA2876499C (fr) | 2012-07-12 | 2021-10-26 | Imstem Biotechnology, Inc. | Cellules souches de type mesenchymateuses issues de cellules souches embryonnaires humaines, leurs procedes et leurs utilisations |
| ES2896354T3 (es) | 2012-12-21 | 2022-02-24 | Astellas Inst For Regenerative Medicine | Métodos para la producción de plaquetas a partir de células madre pluripotentes |
| US9763983B2 (en) | 2013-02-05 | 2017-09-19 | Anthrogenesis Corporation | Natural killer cells from placenta |
| US10975353B2 (en) | 2014-06-10 | 2021-04-13 | Polybiocept Gmbh | Culture medium for cellular immunotherapy |
| US12146161B2 (en) | 2015-02-11 | 2024-11-19 | The Hosptial For Sick Children | Mammalian alveolar macrophages derived from pluripotent cells |
| EP3256569B2 (fr) | 2015-02-11 | 2024-04-17 | The Hospital for Sick Children | Macrophages de type alvéolaire et leur procédé de production |
| CN107922922A (zh) | 2015-08-24 | 2018-04-17 | 维特罗利夫瑞典股份公司 | 培养基 |
| WO2017046142A1 (fr) * | 2015-09-15 | 2017-03-23 | Stichting Katholieke Universiteit | Procédé amélioré pour l'expansion ex vivo de cellules souches hématopoïétiques cd34+ en cellules nk en utilisant un antagoniste de récepteur d'hydrocarbure aryle |
| CA3089853A1 (fr) | 2018-02-01 | 2019-08-08 | Nkmax Co., Ltd. | Procede de production de cellules tueuses naturelles et composition pour le traitement du cancer |
| CA3134782A1 (fr) | 2019-03-28 | 2020-10-01 | The Hospital For Sick Children | Procede de production d'hemangioblastes |
| EP4114415A1 (fr) | 2020-03-06 | 2023-01-11 | Sorrento Therapeutics, Inc. | Cellules tueuses d'immunité naturelle ciblant des cellules tumorales positives au psma |
| CN113801846B (zh) * | 2021-09-22 | 2024-06-04 | 南京艾尔普再生医学科技有限公司 | 一种从人诱导多能干细胞分化为自然杀伤细胞的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5206223A (en) * | 1986-06-26 | 1993-04-27 | Yeda Research And Development Co. Ltd. | Method for inhibiting heparanase activity |
| US5199942A (en) * | 1991-06-07 | 1993-04-06 | Immunex Corporation | Method for improving autologous transplantation |
| DE69739951D1 (de) * | 1996-03-04 | 2010-09-16 | Calyx Bio Ventures Inc | Modifizierte schnellvermehrungsmethode ('modified-rem') zur in vitro vermehrung von t-lymphozyten |
| CA2448599A1 (fr) * | 2001-04-27 | 2002-11-07 | Invitrogen Corporation | Maturation des cellules presentatrices des antigenes au moyen de lymphocytes t actives |
-
2006
- 2006-09-28 EP EP20060799473 patent/EP1941027B1/fr active Active
- 2006-09-28 US US12/088,471 patent/US9109202B2/en active Active
- 2006-09-28 WO PCT/NL2006/000484 patent/WO2007037682A1/fr not_active Ceased
- 2006-09-28 DK DK06799473T patent/DK1941027T3/da active
- 2006-09-28 PL PL06799473T patent/PL1941027T3/pl unknown
- 2006-09-28 PT PT06799473T patent/PT1941027E/pt unknown
- 2006-09-28 SI SI200631825T patent/SI1941027T1/sl unknown
- 2006-09-28 ES ES06799473.1T patent/ES2502841T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1941027A1 (fr) | 2008-07-09 |
| PT1941027E (pt) | 2014-10-09 |
| US20090017539A1 (en) | 2009-01-15 |
| EP1941027B1 (fr) | 2014-08-20 |
| WO2007037682A1 (fr) | 2007-04-05 |
| US9109202B2 (en) | 2015-08-18 |
| DK1941027T3 (da) | 2014-10-13 |
| SI1941027T1 (sl) | 2014-11-28 |
| PL1941027T3 (pl) | 2015-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2502841T3 (es) | Métodos y medios para la proliferación de células madres y generación y expansión posterior de células progenitoras, así como producción de células efectoras como terapéuticos clínicos | |
| ES2638435T3 (es) | Métodos y medios para proliferación de células madre y posterior generación y expansión de células progenitoras, así como también producción de células efectoras como terapéuticos clínicos | |
| ES2666746T3 (es) | Métodos para generar linfocitos citolíticos naturales | |
| JP5283120B2 (ja) | Es細胞からの造血前駆細胞を内包する構造物、及び該構造物を用いた血球細胞の調製方法。 | |
| AU2013302853A1 (en) | Natural killer cells and uses thereof | |
| US9518250B2 (en) | Method for the ex vivo expansion of hematopoietic stem and progenitor cells | |
| JP5597904B2 (ja) | 多能性幹細胞からの血液細胞分化に関する培養方法 | |
| Chivu et al. | The comparison of different protocols for expansion of umbilical‐cord blood hematopoietic stem cells | |
| Hsu et al. | Large-scale production and directed induction of functional dendritic cells ex vivo from serum-free expanded human hematopoietic stem cells | |
| Kwok et al. | Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34+ cells | |
| US20060024827A1 (en) | Stroma-free, serum-free, and chemically defined medium and method for ex vivo mononuclear cell expansion using the same | |
| JP2012165660A (ja) | ヒト造血幹細胞を増幅させるための組成物及び方法 | |
| JP2008237136A (ja) | ヒト造血細胞の培養方法 | |
| Chivu et al. | Ex vivo differentiation of umbilical cord blood progenitor cells in the presence of placental conditioned medium | |
| WO2005085426A1 (fr) | Milieu en vue d'une différenciation exempte d'alimentateur et procédé de différenciation exempt d'alimentateur à partir d'une cellule souche embryonnaire de primate | |
| ES2293353T3 (es) | Productos sanguineos procedentes de celulas madre mesenquimales. | |
| JP2006067858A (ja) | 共培養による造血幹細胞の増幅方法 | |
| US10471097B2 (en) | HAR-NDS-derived stem cells, method for separating same, and use thereof | |
| Mesquitta | Investigating an Effect of the Pyrimido-indole Derivative, UM171, on Human Pluripotent Stem Cell-derived Hematopoietic Progenitor Cells | |
| Cerdan et al. | Hematopoietic Differentiation | |
| US20080233643A1 (en) | Differentiation of Cd34 Positive Cell to Megakaryocyte and Multiplication | |
| Bock et al. | Clinical use for expanded peripheral blood stem cells | |
| da Mata | Stem cell engineering approaches towards angiogenic cell therapy for ischemic diseases |