ES2530757T3 - Método de expansión de condrocitos con conservación fenotípica - Google Patents
Método de expansión de condrocitos con conservación fenotípica Download PDFInfo
- Publication number
- ES2530757T3 ES2530757T3 ES08831789T ES08831789T ES2530757T3 ES 2530757 T3 ES2530757 T3 ES 2530757T3 ES 08831789 T ES08831789 T ES 08831789T ES 08831789 T ES08831789 T ES 08831789T ES 2530757 T3 ES2530757 T3 ES 2530757T3
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- Prior art keywords
- chondrocytes
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- cartilaginous
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- tissue
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- 210000001612 chondrocyte Anatomy 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 2
- 238000004321 preservation Methods 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 6
- 210000001519 tissue Anatomy 0.000 abstract 5
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 229920002674 hyaluronan Polymers 0.000 abstract 2
- 229960003160 hyaluronic acid Drugs 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 239000004417 polycarbonate Substances 0.000 abstract 2
- 229920000515 polycarbonate Polymers 0.000 abstract 2
- 239000011148 porous material Substances 0.000 abstract 2
- 239000013587 production medium Substances 0.000 abstract 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 abstract 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000002744 extracellular matrix Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 238000009331 sowing Methods 0.000 abstract 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/80—Hyaluronan
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Rheumatology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Un método para producir tejido cartilaginoso in vitro, comprendiendo el método: a) aislar una población de condrocitos de tejido cartilaginoso donante aislado; b) expandir la población de condrocitos cartilaginosos en un medio de expansión y en un sustrato en condiciones eficaces para impedir la adhesión al sustrato de una mayoría de los condrocitos, en el que las condiciones comprenden expandir la población de células usando condiciones que comprenden: (i) un sustrato que tiene un recubrimiento de ácido hialurónico; o (ii) un sustrato no modificado que usa un medio de expansión que comprende ácido hialurónico en solución; c) sembrar una pluralidad de condrocitos de la población expandida de condrocitos cartilaginosos en un medio de producción tisular que comprenda TGF-β, en un sustrato de policarbonato que tenga una pluralidad de poros a través del mismo, teniendo los poros un diámetro interno de al menos 1 micra a aproximadamente 12 micras; y d) cultivar los condrocitos sembrados en el medio de producción tisular en el sustrato de policarbonato poroso durante un periodo de tiempo suficiente para que los condrocitos produzcan matriz extracelular, produciendo de este modo in vivo un tejido cartilaginoso cultivado caracterizado por una organización de células al azar.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/859,524 US8017394B2 (en) | 2004-10-01 | 2007-09-21 | Method for chondrocyte expansion with phenotype retention |
| PCT/US2008/077161 WO2009039469A1 (en) | 2007-09-21 | 2008-09-22 | Method for chondrocyte expansion with phenotype retention |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2530757T3 true ES2530757T3 (es) | 2015-03-05 |
Family
ID=40469879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES08831789T Active ES2530757T3 (es) | 2007-09-21 | 2008-09-22 | Método de expansión de condrocitos con conservación fenotípica |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8017394B2 (es) |
| EP (1) | EP2201099B1 (es) |
| JP (1) | JP2010539905A (es) |
| AU (1) | AU2008302088B2 (es) |
| DK (1) | DK2201099T3 (es) |
| ES (1) | ES2530757T3 (es) |
| WO (1) | WO2009039469A1 (es) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0896825B1 (en) * | 1997-08-14 | 2002-07-17 | Sulzer Innotec Ag | Composition and device for in vivo cartilage repair comprising nanocapsules with osteoinductive and/or chondroinductive factors |
| US7622562B2 (en) | 2002-06-26 | 2009-11-24 | Zimmer Orthobiologics, Inc. | Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue |
| EP2335650B1 (en) | 2003-12-11 | 2012-10-03 | Isto Technologies Inc. | Particulate cartilage system |
| US8889127B2 (en) | 2004-07-01 | 2014-11-18 | Icahn School Of Medicine At Mount Sinai | Targeted protein replacement for the treatment of lysosomal storage disorders |
| WO2007025290A2 (en) | 2005-08-26 | 2007-03-01 | Isto Technologies, Inc. | Implants and methods for repair, replacement and treatment of joint disease |
| EP1764117A1 (en) * | 2005-09-20 | 2007-03-21 | Zimmer GmbH | Implant for the repair of a cartilage defect and method for manufacturing the implant |
| US20080154233A1 (en) * | 2006-12-20 | 2008-06-26 | Zimmer Orthobiologics, Inc. | Apparatus for delivering a biocompatible material to a surgical site and method of using same |
| US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
| EP2134297B1 (en) | 2007-04-12 | 2017-03-08 | Zimmer, Inc. | Apparatus for tissue repair |
| US8426196B2 (en) * | 2008-06-05 | 2013-04-23 | Lynn L. H. Huang | Method for regulating proliferation of cells |
| US20100041149A1 (en) * | 2008-08-15 | 2010-02-18 | Mark Weidenbecher | Bioreactor and method for generating cartilage tissue constructs |
| US11248211B2 (en) * | 2008-11-25 | 2022-02-15 | Kolon Tissuegene, Inc. | Primed cell therapy |
| JP5721319B2 (ja) * | 2009-08-17 | 2015-05-20 | 国立大学法人北海道大学 | 軟骨細胞培養基材及び培養方法 |
| JP5544635B2 (ja) * | 2009-09-30 | 2014-07-09 | 国立大学法人北海道大学 | 軟骨細胞再分化誘導用基材およびこれを用いた軟骨細胞の製造方法 |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| GB201010422D0 (en) * | 2010-06-22 | 2010-08-04 | Univ Cardiff | Cartilage repair |
| US8834928B1 (en) | 2011-05-16 | 2014-09-16 | Musculoskeletal Transplant Foundation | Tissue-derived tissugenic implants, and methods of fabricating and using same |
| WO2013003595A1 (en) | 2011-06-29 | 2013-01-03 | Biorestorative Therapies, Inc. | Brown fat cell compositions and methods |
| EP3741384A1 (en) | 2011-09-07 | 2020-11-25 | Mount Sinai School Of Medicine | Ceramidase and cell differentiation |
| CN109529028A (zh) | 2012-06-01 | 2019-03-29 | 西奈山伊坎医学院 | 在感染的治疗和预防中的神经酰胺水平 |
| EP2875125B1 (en) * | 2012-07-20 | 2018-10-24 | Alfred C. Kuo | Methods for producing cartilage and bone |
| US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
| WO2014160390A1 (en) | 2013-03-14 | 2014-10-02 | Icahn School Of Medicine At Mount Sinai | Therapeutic acid ceramidase compositions and methods of making and using them |
| ES2957410T3 (es) | 2013-04-19 | 2024-01-18 | Biorestorative Therapies Inc | Células madre derivadas de tejido adiposo marrón humano y sus utilizaciones |
| JP6485349B2 (ja) * | 2013-06-07 | 2019-03-20 | 日産化学株式会社 | 細胞培養器 |
| WO2016019168A1 (en) * | 2014-08-01 | 2016-02-04 | Isto Technologies, Inc. | Cell expansion methods and systems |
| EP4509147A2 (en) | 2014-08-01 | 2025-02-19 | Isto Technologies, Inc. | Cartilage compositions and methods for modifying proteoglycan content |
| EP3297694A1 (en) | 2015-05-21 | 2018-03-28 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
| EP3473701B1 (en) | 2016-06-15 | 2021-03-03 | Nissan Chemical Corporation | Container for cryopreservation |
| WO2018021362A1 (ja) * | 2016-07-25 | 2018-02-01 | 宇部興産株式会社 | 脱分化しやすい細胞の脱分化を抑制する方法、当該細胞の調製方法、及び物質の産生方法 |
| US12291701B2 (en) | 2017-09-26 | 2025-05-06 | Nissan Chemical Corporation | Cell culture container having minute volume |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4356261A (en) * | 1980-04-22 | 1982-10-26 | Rush-Presbyterian-St. Luke's Medical Center | Anti-invasion factor containing cultures |
| IT1219587B (it) * | 1988-05-13 | 1990-05-18 | Fidia Farmaceutici | Polisaccaridi carbossiilici autoreticolati |
| US5326357A (en) * | 1992-03-18 | 1994-07-05 | Mount Sinai Hospital Corporation | Reconstituted cartridge tissue |
| IT1263316B (it) * | 1993-02-12 | 1996-08-05 | Fidia Advanced Biopolymers Srl | Tessuto non tessuto multistrato in cui uno degli strati e' costituito essenzialmente da esteri dell'acido ialuronico |
| US5723331A (en) * | 1994-05-05 | 1998-03-03 | Genzyme Corporation | Methods and compositions for the repair of articular cartilage defects in mammals |
| JP3028852B2 (ja) * | 1994-06-24 | 2000-04-04 | ロシュ ダイアグノスティックス コーポレーション | ヒストリチクス菌から得られるコラゲナーゼおよび２つの他のプロテアーゼの精製混合物 |
| US5716404A (en) * | 1994-12-16 | 1998-02-10 | Massachusetts Institute Of Technology | Breast tissue engineering |
| IT1288290B1 (it) * | 1996-06-21 | 1998-09-11 | Fidia Spa In Amministrazione S | Acido ialuronico autoreticolato e relative composizioni farmaceutiche per il trattamento delle artropatie |
| EP0920490A2 (en) * | 1996-07-25 | 1999-06-09 | Genzyme Corporation | Chondrocyte media formulations and culture procedures |
| US5830741A (en) * | 1996-12-06 | 1998-11-03 | Boehringer Mannheim Corporation | Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease |
| US5866165A (en) * | 1997-01-15 | 1999-02-02 | Orquest, Inc. | Collagen-polysaccharide matrix for bone and cartilage repair |
| EP1018987B1 (en) * | 1997-04-04 | 2014-10-29 | Barnes-Jewish Hospital | Neocartilage and methods of use |
| WO1998059035A2 (en) * | 1997-06-25 | 1998-12-30 | The Goverment Of The United States Of America, Represented By The Secretary, Dept. Of Health And Human Services, National Institutes Of Health | Serum-free cell growth medium |
| EP1112348B1 (en) * | 1998-09-18 | 2005-11-30 | Massachusetts Institute Of Technology | Use of growth factors and hormones for expansion of mammalian cells and tissue engineering |
| IT1302534B1 (it) * | 1998-12-21 | 2000-09-05 | Fidia Advanced Biopolymers Srl | Composizioni iniettabili, biocompatibili e biodegradabili comprendentialmeno un derivato dell'acido ialuronico, cellule condrogeniche, per |
| US6197061B1 (en) * | 1999-03-01 | 2001-03-06 | Koichi Masuda | In vitro production of transplantable cartilage tissue cohesive cartilage produced thereby, and method for the surgical repair of cartilage damage |
| AU1618201A (en) * | 1999-11-19 | 2001-05-30 | Children's Medical Center Corporation | Methods for inducing chondrogenesis and producing de novo cartilage in vitro |
| US6932977B2 (en) * | 2000-04-14 | 2005-08-23 | Depuy Spine, Inc. | Method of inducing or enhancing chondrogenesis with extracellular matrix containing BMP-4 |
| US7476257B2 (en) * | 2001-09-15 | 2009-01-13 | Rush University Medical Center | Methods to engineer stratified cartilage tissue |
| CA2479840A1 (en) * | 2002-04-02 | 2003-10-16 | William Marsh Rice University | Redifferentiated cells for repairing cartilage defects |
| US7074615B2 (en) * | 2003-08-15 | 2006-07-11 | Becton, Dickinson And Company | Peptides for enhanced cell attachment and cell growth |
| US7273756B2 (en) * | 2004-10-01 | 2007-09-25 | Isto Technologies, Inc. | Method for chondrocyte expansion with phenotype retention |
| CN101500512B (zh) | 2006-02-07 | 2011-08-24 | 脊柱赛特有限公司 | 使用生物反应器的修复软骨的方法和组合物 |
-
2007
- 2007-09-21 US US11/859,524 patent/US8017394B2/en active Active
-
2008
- 2008-09-22 AU AU2008302088A patent/AU2008302088B2/en active Active
- 2008-09-22 EP EP08831789.6A patent/EP2201099B1/en not_active Not-in-force
- 2008-09-22 WO PCT/US2008/077161 patent/WO2009039469A1/en not_active Ceased
- 2008-09-22 DK DK08831789.6T patent/DK2201099T3/da active
- 2008-09-22 ES ES08831789T patent/ES2530757T3/es active Active
- 2008-09-22 JP JP2010526037A patent/JP2010539905A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010539905A (ja) | 2010-12-24 |
| EP2201099A4 (en) | 2011-06-01 |
| AU2008302088B2 (en) | 2014-02-20 |
| EP2201099B1 (en) | 2014-11-19 |
| WO2009039469A1 (en) | 2009-03-26 |
| AU2008302088A1 (en) | 2009-03-26 |
| EP2201099A1 (en) | 2010-06-30 |
| US20080081369A1 (en) | 2008-04-03 |
| DK2201099T3 (da) | 2015-02-23 |
| US8017394B2 (en) | 2011-09-13 |
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