ES2531459A1 - Método para la separación física de "traductomas" convolucionados - Google Patents

Método para la separación física de "traductomas" convolucionados Download PDF

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Publication number
ES2531459A1
ES2531459A1 ES201300566A ES201300566A ES2531459A1 ES 2531459 A1 ES2531459 A1 ES 2531459A1 ES 201300566 A ES201300566 A ES 201300566A ES 201300566 A ES201300566 A ES 201300566A ES 2531459 A1 ES2531459 A1 ES 2531459A1
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traductomas
rebivas
partners
translation
machine
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ES2531459B1 (es
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Antonio VILA SANJURJO
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método para la separación física de "traductomas" convolucionados. Este método se basa en una rápida lisis para todos los socios presentes en ReBiVaS (relaciones biológicas de varios socios) y ReBiVaS-AD, (relaciones biológicas de varios socios altamente desequilibradas) seguida de un procedimiento de aislamiento que permita la separación de los "traductomas" individuales de cada uno de los socios. Los diferentes "traductomas" involucrados en ReBiVaS y ReBiVaS-AD son capturados por medio de un número igual de tipos de anticuerpos con especificidad hacia una proteína-r específica de cada uno de dichos "traductomas". Tras su captura en resinas de cromatografía independientes y colocadas en tandem, dichos "traductomas" son eluídos por separado. Se propone el desarrollo de anticuerpos específicos para zonas no conservadas de proteínas ribosómicas (proteínas-r) de modo que diferentes "traductomas" presentes en una misma muestra puedan ser fácilmente separados.

Description

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Claims (1)

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ES201300566A 2013-06-10 2013-06-10 Método para la separación física de "traductomas" convolucionados Expired - Fee Related ES2531459B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300566A ES2531459B1 (es) 2013-06-10 2013-06-10 Método para la separación física de "traductomas" convolucionados

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ES201300566A ES2531459B1 (es) 2013-06-10 2013-06-10 Método para la separación física de "traductomas" convolucionados

Publications (2)

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ES2531459A1 true ES2531459A1 (es) 2015-03-16
ES2531459B1 ES2531459B1 (es) 2015-12-28

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ES201300566A Expired - Fee Related ES2531459B1 (es) 2013-06-10 2013-06-10 Método para la separación física de "traductomas" convolucionados

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2170788T3 (es) * 1995-09-22 2002-08-16 Zlb Bioplasma Ag Procedimiento para la obtencion de inmunoglobulinas a partir de fracciones que resultan de plasma sanguineo humano.
ES2177632T3 (es) * 1994-02-22 2002-12-16 Smithkline Beecham Corp Purificacion de anticuerpos.
WO2012123520A1 (en) * 2011-03-17 2012-09-20 Chromacon Ag Method for identification and purification of multi-specific polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2177632T3 (es) * 1994-02-22 2002-12-16 Smithkline Beecham Corp Purificacion de anticuerpos.
ES2170788T3 (es) * 1995-09-22 2002-08-16 Zlb Bioplasma Ag Procedimiento para la obtencion de inmunoglobulinas a partir de fracciones que resultan de plasma sanguineo humano.
WO2012123520A1 (en) * 2011-03-17 2012-09-20 Chromacon Ag Method for identification and purification of multi-specific polypeptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GUERRERO HY et al. Evaluación de dos métodos para la separación de ARN mensajero de Plasmodium falciparum. Biomédica. 1998. Vol. 18(1), páginas: 55-65, página 55, resumen. *
LEONOV AA et al. Affinity Purification of Ribosomes with a Lethal G2655 Mutation in 23 S rRNA That Affects the Translocation. The Journal of Biological Chemistry. 2003. Vol. 278 (28), páginas: 25664-25670, página 25664, resumen . *

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Publication number Publication date
ES2531459B1 (es) 2015-12-28

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