ES2532030A1 - Método de medición del tono vascular ex vivo y cámara de flujo - Google Patents

Método de medición del tono vascular ex vivo y cámara de flujo Download PDF

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Publication number
ES2532030A1
ES2532030A1 ES201300866A ES201300866A ES2532030A1 ES 2532030 A1 ES2532030 A1 ES 2532030A1 ES 201300866 A ES201300866 A ES 201300866A ES 201300866 A ES201300866 A ES 201300866A ES 2532030 A1 ES2532030 A1 ES 2532030A1
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ES
Spain
Prior art keywords
vascular tone
flow chamber
measuring
vessel
vivo vascular
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Granted
Application number
ES201300866A
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ES2532030B2 (es
Inventor
María Teresa TEJERINA SÁNCHEZ
Santiago REDONDO BLASCO
Úrsula Fabiola MEDINA MORENO
Jorge NAVARRO DORADO
Marta RAMAJO MATESANZ
Alexis CANTIZANO GONZÁLEZ
Mario CASTRO PONCE
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Universidad Complutense de Madrid
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Universidad Complutense de Madrid
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Priority to ES201300866A priority Critical patent/ES2532030B2/es
Publication of ES2532030A1 publication Critical patent/ES2532030A1/es
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Publication of ES2532030B2 publication Critical patent/ES2532030B2/es
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método de medición del tono vascular ex vivo y cámara de flujo. La presente invención se refiere a un método de medición del tono vascular ex vivo que comprende: perfundir un flujo constante de una suspensión celular en un circuito abierto que incluye un vaso cuyo tono se desea estudiar, cerrar el circuito, perfundir una solución nutricia de Krebs en el circuito cerrado y aplicar una presión transmural constante, añadir una sustancia vasoactiva y registrar las variaciones de diámetro del vaso mediante un sistema de captación de imágenes situado perpendicularmente al vaso y acoplado a un microscopio. La invención también se refiere a una cámara de flujo en la que realizar el método de medición del tono vascular que incluye dos capilares de vidrio, un baño, una bomba de infusión, un sistema de presión hidrostática, un manómetro, un sistema de termorregulación de la temperatura del baño y de las soluciones y/o suspensiones utilizadas, un sistema de captación de imágenes y un microscopio.

Description

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Claims (1)

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ES201300866A 2013-09-20 2013-09-20 Método de medición del tono vascular ex vivo y cámara de flujo Active ES2532030B2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (es) 2013-09-20 2013-09-20 Método de medición del tono vascular ex vivo y cámara de flujo

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (es) 2013-09-20 2013-09-20 Método de medición del tono vascular ex vivo y cámara de flujo

Publications (2)

Publication Number Publication Date
ES2532030A1 true ES2532030A1 (es) 2015-03-23
ES2532030B2 ES2532030B2 (es) 2015-12-28

Family

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ES201300866A Active ES2532030B2 (es) 2013-09-20 2013-09-20 Método de medición del tono vascular ex vivo y cámara de flujo

Country Status (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (zh) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 一种血管管径检测设备

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (zh) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 一种血管管径检测设备

Also Published As

Publication number Publication date
ES2532030B2 (es) 2015-12-28

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