ES2532030B2 - Method of measuring ex vivo vascular tone and flow chamber - Google Patents

Method of measuring ex vivo vascular tone and flow chamber Download PDF

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Publication number
ES2532030B2
ES2532030B2 ES201300866A ES201300866A ES2532030B2 ES 2532030 B2 ES2532030 B2 ES 2532030B2 ES 201300866 A ES201300866 A ES 201300866A ES 201300866 A ES201300866 A ES 201300866A ES 2532030 B2 ES2532030 B2 ES 2532030B2
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Spain
Prior art keywords
measuring
vascular tone
flow chamber
vessel
tone
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Active
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ES201300866A
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Spanish (es)
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ES2532030A1 (en
Inventor
María Teresa TEJERINA SÁNCHEZ
Santiago REDONDO BLASCO
Úrsula Fabiola MEDINA MORENO
Jorge NAVARRO DORADO
Marta RAMAJO MATESANZ
Alexis CANTIZANO GONZÁLEZ
Mario CASTRO PONCE
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Universidad Complutense de Madrid
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Universidad Complutense de Madrid
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Priority to ES201300866A priority Critical patent/ES2532030B2/en
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método de medición del tono vascular ex vivo y cámara de flujo.#La presente invención se refiere a un método de medición del tono vascular ex vivo que comprende: perfundir un flujo constante de una suspensión celular en un circuito abierto que incluye un vaso cuyo tono se desea estudiar, cerrar el circuito, perfundir una solución nutricia de Krebs en el circuito cerrado y aplicar una presión transmural constante, añadir una sustancia vasoactiva y registrar las variaciones de diámetro del vaso mediante un sistema de captación de imágenes situado perpendicularmente al vaso y acoplado a un microscopio.#La invención también se refiere a una cámara de flujo en la que realizar el método de medición del tono vascular que incluye dos capilares de vidrio, un baño, una bomba de infusión, un sistema de presión hidrostática, un manómetro, un sistema de termorregulación de la temperatura del baño y de las soluciones y/o suspensiones utilizadas, un sistema de captación de imágenes y un microscopio.Method of measuring ex vivo vascular tone and flow chamber # The present invention relates to a method of measuring ex vivo vascular tone comprising: perfusing a constant flow of a cell suspension in an open circuit that includes a vessel whose tone it is desired to study, close the circuit, perfuse a nutrient solution of Krebs in the closed circuit and apply a constant transmural pressure, add a vasoactive substance and record the vessel diameter variations by means of an image capture system located perpendicular to the vessel and coupled to a microscope. # The invention also relates to a flow chamber in which to perform the method of measuring vascular tone that includes two glass capillaries, a bath, an infusion pump, a hydrostatic pressure system, a pressure gauge, a thermoregulation system for the bath temperature and the solutions and / or suspensions used, a system for capturing i images and a microscope.

Description

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Claims (1)

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ES201300866A 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber Active ES2532030B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Publications (2)

Publication Number Publication Date
ES2532030A1 ES2532030A1 (en) 2015-03-23
ES2532030B2 true ES2532030B2 (en) 2015-12-28

Family

ID=52683008

Family Applications (1)

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ES201300866A Active ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

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ES (1) ES2532030B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878B (en) * 2023-03-07 2023-06-20 中国人民解放军军事科学院军事医学研究院 A method for detecting changes in vascular diameter

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
DE3774680D1 (en) * 1986-12-22 1992-01-02 Siemens Ag ARRANGEMENT FOR EXAMINING A LIQUID MEDIUM AND METHOD FOR OPERATING THE ARRANGEMENT.
US5078135A (en) * 1989-05-31 1992-01-07 Board Of Regents, The University Of Texas System Apparatus for in vivo analysis of biological compounds in blood or tissue by microdialysis and mass spectrometry
US6607501B2 (en) * 2001-05-14 2003-08-19 Reynolds G. Gorsuch Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
CA2605233C (en) * 2005-04-29 2013-04-23 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Also Published As

Publication number Publication date
ES2532030A1 (en) 2015-03-23

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