ES2540588T3 - Método para mejorar la eficiencia del uso de agua en plantas - Google Patents

Método para mejorar la eficiencia del uso de agua en plantas Download PDF

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Publication number
ES2540588T3
ES2540588T3 ES11155855.7T ES11155855T ES2540588T3 ES 2540588 T3 ES2540588 T3 ES 2540588T3 ES 11155855 T ES11155855 T ES 11155855T ES 2540588 T3 ES2540588 T3 ES 2540588T3
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Prior art keywords
plants
efficiency
improve
water use
transgenic
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Expired - Lifetime
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ES11155855.7T
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English (en)
Inventor
Shimon Gepstein
Amira Gepstein
Eduardo Blumwald
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Technion Research and Development Foundation Ltd
University of California Berkeley
University of California San Diego UCSD
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Technion Research and Development Foundation Ltd
University of California Berkeley
University of California San Diego UCSD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Peptides Or Proteins (AREA)
  • Cultivation Of Plants (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método de aumento de la eficiencia de uso del agua por una planta, comprendiendo el método: (a) transformar una población de plantas con una casete de expresión recombinante que comprende un promotor SARK inducible por senescencia enlazado operativamente a una secuencia de ácido nucleico que codifica isopentenil-transferasa; y (b) seleccionar una planta que tiene una eficiencia de uso del agua incrementada, en donde el promotor SARK es (i) al menos 95% idéntico al promotor de SEQ ID NO: 1 o (ii) al menos 95% idéntico a 800 pb del extremo 5' de SEQ ID NO: 1.

Description

imagen1
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E11155855
24-06-2015
completo ambos linajes transgénicos (Figs. 2K y 2L). Las medidas del peso fresco de las plantas de tipo salvaje y transgénicas a finales del periodo de rehidratación demostraron que los linajes transgénicos alcanzaban un peso fresco de ~250 gramos/planta, mientras que el tipo salvaje seguía seco, con un peso que no excedía del 20% del correspondiente a los linajes transgénicos (Fig. 2). La Figura 3 muestra el peso fresco
5 de las plantas representadas en Fig. 2 después de 14 días de rehidratación. Los valores son la media ± SD (n = 40).
La expresión del gen IPT bajo la regulación del promotor del gen SARK confiere resistencia a la sequía a las
plantas transgénicas de Arabidopsis
Se cultivaron plantas de Arabidopsis thaliana bajo un régimen de día largo (16/8 horas) a 23 ºC. No pudieron
10 distinguirse diferencias morfológicas y de desarrollo de ningún tipo entre las plantas WT y las plantas transgénicas (pSARK:IPT) cultivadas en condiciones normales. En cambio, cuando se sometieron plantas de dos meses (en la etapa de floración avanzada) a estrés de sequía (sin adición alguna de agua a los tiestos) exhibían resistencia diferencial al estrés. Las plantas WT sufrieron marchitamiento severo irreversible y amarilleo de las hojas después de 12 días de deshidratación, mientras que 10 linajes independientes diferentes
15 de las plantas transgénicas T1 (pSARK:IPT) exhibían amarilleo moderado y se recuperaban del estrés de sequía después de 5 días de rehidratación (Fig. 4).
8
imagen7
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Claims (1)

  1. imagen1
ES11155855.7T 2005-03-21 2006-03-21 Método para mejorar la eficiencia del uso de agua en plantas Expired - Lifetime ES2540588T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66403505P 2005-03-21 2005-03-21
US664035P 2005-03-21

Publications (1)

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ES2540588T3 true ES2540588T3 (es) 2015-07-10

Family

ID=37024661

Family Applications (2)

Application Number Title Priority Date Filing Date
ES06739467T Expired - Lifetime ES2376924T3 (es) 2005-03-21 2006-03-21 Métodos para generar plantas resistentes a la sequ�?a.
ES11155855.7T Expired - Lifetime ES2540588T3 (es) 2005-03-21 2006-03-21 Método para mejorar la eficiencia del uso de agua en plantas

Family Applications Before (1)

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ES06739467T Expired - Lifetime ES2376924T3 (es) 2005-03-21 2006-03-21 Métodos para generar plantas resistentes a la sequ�?a.

Country Status (13)

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US (1) US9624503B2 (es)
EP (2) EP2324703B1 (es)
CN (1) CN101146439B (es)
AT (1) ATE531807T1 (es)
AU (2) AU2006226863B2 (es)
BR (1) BRPI0607732B1 (es)
CA (1) CA2601605C (es)
ES (2) ES2376924T3 (es)
HU (1) HUE025741T2 (es)
MX (1) MX2007011612A (es)
PL (2) PL1863334T3 (es)
WO (1) WO2006102559A2 (es)
ZA (1) ZA200707905B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2674217A1 (en) * 2006-12-08 2008-06-12 The University Of York Regulation of plant metabolism
EP2593554A2 (en) * 2010-07-15 2013-05-22 Technion Research And Development Foundation Ltd. Nucleic acid construct for increasing abiotic stress tolerance in plants
US10031531B2 (en) 2011-02-25 2018-07-24 Mks Instruments, Inc. System for and method of multiple channel fast pulse gas delivery
US10126760B2 (en) 2011-02-25 2018-11-13 Mks Instruments, Inc. System for and method of fast pulse gas delivery
CN104884619B (zh) * 2012-12-31 2017-09-08 创世纪种业有限公司 一种棉花异戊烯基转移酶ipt2及其编码基因与应用
WO2017083757A1 (en) 2015-11-13 2017-05-18 Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Reno Methods of engineered tissue succulence in plants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5306862A (en) 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
US5837851A (en) 1994-12-08 1998-11-17 Pioneer Hi-Bred International, Inc. DNA promoter 5126 and constructs useful in a reversible nuclear genetic system for male sterility in transgenic plants
US5689042A (en) 1995-03-29 1997-11-18 Wisconsin Alumni Research Foundation Transgenic plants with altered senescence characteristics
EP1045631A1 (en) * 1997-12-08 2000-10-25 Vitality Biotechnologies, Inc. Plant senescence-associated genes
US20050086718A1 (en) 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
AU2001259754A1 (en) 2000-05-10 2001-11-20 Phycogen, Inc. Transgenic plants incorporating_genes of zostera marina
AUPQ994600A0 (en) 2000-09-06 2000-09-28 Agriculture Victoria Services Pty Ltd Manipulation of plant senescene
ES2392190T3 (es) * 2001-12-10 2012-12-05 Thomas Schmulling Método para modificar la morfología, bioquímica y fisiología de plantas que comprende la expresión de citoquinina oxidasa de plantas
MXPA04009778A (es) * 2002-04-08 2004-12-13 Pionner Hi Bred International Exsercion de fibra sedosa mejorada bajo estres.
US7138566B2 (en) 2002-06-21 2006-11-21 Regents Of The University Of California Methods of modulating cytokinin related processes in a plant using B3 domain proteins

Also Published As

Publication number Publication date
PL2324703T3 (pl) 2015-11-30
EP1863334A2 (en) 2007-12-12
EP2324703B1 (en) 2015-05-06
CN101146439B (zh) 2013-03-27
EP1863334B1 (en) 2011-11-02
EP1863334A4 (en) 2008-11-26
BRPI0607732B1 (pt) 2017-11-07
PL1863334T3 (pl) 2012-04-30
CN101146439A (zh) 2008-03-19
AU2006226863A1 (en) 2006-09-28
ES2376924T3 (es) 2012-03-20
ZA200707905B (en) 2008-12-31
CA2601605C (en) 2014-09-09
AU2011202138B2 (en) 2011-11-03
HUE025741T2 (en) 2016-04-28
BRPI0607732A2 (pt) 2010-03-16
WO2006102559A3 (en) 2007-02-22
US20080282365A1 (en) 2008-11-13
US9624503B2 (en) 2017-04-18
EP2324703A1 (en) 2011-05-25
MX2007011612A (es) 2007-10-18
ATE531807T1 (de) 2011-11-15
CA2601605A1 (en) 2006-09-28
AU2006226863B2 (en) 2011-02-10
AU2011202138A1 (en) 2011-05-26
WO2006102559A2 (en) 2006-09-28

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