ES2543629T3 - Células productoras de polipéptidos - Google Patents
Células productoras de polipéptidos Download PDFInfo
- Publication number
- ES2543629T3 ES2543629T3 ES10154684.4T ES10154684T ES2543629T3 ES 2543629 T3 ES2543629 T3 ES 2543629T3 ES 10154684 T ES10154684 T ES 10154684T ES 2543629 T3 ES2543629 T3 ES 2543629T3
- Authority
- ES
- Spain
- Prior art keywords
- immunoglobulin
- nucleic acid
- mrna
- domain
- processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de selección de una célula eucariótica expresante de una inmunoglobulina, en el que el método comprende: a) transfectar una célula eucariótica con un ácido nucleico que comprende, en dirección 5' a 3', i) un primer ácido nucleico codificante de por lo menos un fragmento de un dominio CH3 o CH4 de cadena pesada de inmunoglobulina sin codón de parada traduccional dentro del marco, ii) un segundo ácido nucleico que se inicia con el sitio 5' donante de procesamiento de un dominio CH3 o CH4 de cadena pesada de inmunoglobulina y que termina en el sitio 3' aceptor de procesamiento del exón M1 de dominio transmembranal de cadena pesada de inmunoglobulina posterior que comprende un codón de parada traduccional dentro del marco y una señal de poliadenilación, iii) un tercer ácido nucleico codificante de: iiia) por lo menos un fragmento de un dominio transmembranal de inmunoglobulina, b) cultivar dicha célula transfectada bajo condiciones adecuadas a la producción de pre-ARNm de dicho ácido nucleico, procesar dicho pre-ARNm, y traducción de dicho ARNm procesado en una inmunoglobulina, en la que dicha célula transfectada produce inmunoglobulina soluble e inmunoglobulina unida a membrana plasmática mediante procesamiento alternativo de dicho pre-ARNm y c) seleccionar una célula con inmunoglobulina unida a membrana plasmática que será la célula expresante de una inmunoglobulina.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06010146 | 2006-05-17 | ||
| EP06010146 | 2006-05-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2543629T3 true ES2543629T3 (es) | 2015-08-20 |
Family
ID=37076089
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07725230.2T Active ES2573027T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptido |
| ES10154684.4T Active ES2543629T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptidos |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07725230.2T Active ES2573027T3 (es) | 2006-05-17 | 2007-05-15 | Células productoras de polipéptido |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8354516B2 (es) |
| EP (2) | EP2027273B1 (es) |
| JP (1) | JP4870208B2 (es) |
| KR (1) | KR101202498B1 (es) |
| CN (1) | CN101410525B (es) |
| AU (1) | AU2007251766B2 (es) |
| BR (1) | BRPI0712073B8 (es) |
| CA (1) | CA2651478C (es) |
| ES (2) | ES2573027T3 (es) |
| IL (1) | IL193721A (es) |
| MX (1) | MX2008013514A (es) |
| WO (1) | WO2007131774A1 (es) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2027273B1 (en) * | 2006-05-17 | 2016-04-13 | F. Hoffmann-La Roche AG | Polypeptide producing cells |
| WO2008070367A2 (en) | 2006-11-01 | 2008-06-12 | Facet Biotech Corporation | Mammalian cell-based immunoglobulin display libraries |
| US7947495B2 (en) | 2007-11-01 | 2011-05-24 | Abbott Biotherapeutics Corp. | Immunoglobulin display vectors |
| AU2009286956B2 (en) | 2008-08-28 | 2012-12-06 | Novartis Ag | Cell surface display of polypeptide isoforms by stop codon readthrough |
| JP5801207B2 (ja) | 2009-02-27 | 2015-10-28 | ノバルティス アーゲー | ２種類の選択マーカーを含む発現ベクター系 |
| FR2944016B1 (fr) | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides anti-age activateurs du proteasome et compositions les contenant |
| FR2944018B1 (fr) * | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides anti-age activateurs du proteasome et compositions les contenant |
| FR2944017B1 (fr) * | 2009-04-02 | 2012-03-09 | Isp Investments Inc | Nouveaux peptides eclaircissants activateurs du proteasome et compositions les contenant |
| FR2944798B1 (fr) | 2009-04-23 | 2013-05-10 | Isp Investments Inc | Hydrolysats peptidiques eclaircissants activateurs du proteasome et compositions les contenant |
| FR2944797B1 (fr) | 2009-04-23 | 2013-05-10 | Isp Investments Inc | Hydrolysats peptidiques activateurs du proteasome et compositions les contenant |
| CN101875916B (zh) * | 2009-11-27 | 2013-04-17 | 西安交通大学 | 一种fgfr1高表达的重组hek293细胞及其应用 |
| MX359950B (es) | 2012-11-20 | 2018-10-17 | Novartis Ag | Casete de expresión optimizado para expresar un polipéptido con un alto rendimiento. |
| WO2014126254A1 (ja) * | 2013-02-18 | 2014-08-21 | 協和発酵キリン株式会社 | 膜結合型タンパク質発現ベクター |
| WO2014141037A1 (en) | 2013-03-11 | 2014-09-18 | Novartis Ag | Method of screening cell clones |
| WO2014171826A1 (en) | 2013-04-17 | 2014-10-23 | Stichting Vu-Vumc | Treatment of cognitive impairment in depressive disorders |
| RU2716977C2 (ru) | 2013-07-31 | 2020-03-17 | Новартис Аг | Новые селективные векторы и способы селекции эукариотических клеток-хозяев |
| EP3083677B1 (en) | 2013-12-20 | 2019-08-28 | Novartis AG | Novel eukaryotic cells and methods for recombinantly expressing a product of interest |
| JP6708548B2 (ja) | 2013-12-20 | 2020-06-10 | ノバルティス アーゲー | 新規真核細胞および目的の生成物を組換え発現させるための方法 |
| WO2015128509A1 (en) * | 2014-02-28 | 2015-09-03 | Glenmark Pharmaceuticals S.A. | Expression constructs and methods for selecting host cells expressing polypeptides |
| US11623950B2 (en) | 2015-07-09 | 2023-04-11 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Lentiviral vector expressing membrane-anchored or secreted antibody |
| GB2592821B (en) | 2015-07-31 | 2022-01-12 | Univ Minnesota | Modified cells and methods of therapy |
| CN109790214B (zh) * | 2016-09-29 | 2022-09-06 | 豪夫迈·罗氏有限公司 | 用于选择产生多肽的细胞的改进方法 |
| CN110520530A (zh) | 2016-10-18 | 2019-11-29 | 明尼苏达大学董事会 | 肿瘤浸润性淋巴细胞和治疗方法 |
| EP3645021A4 (en) | 2017-06-30 | 2021-04-21 | Intima Bioscience, Inc. | ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY |
| WO2019015677A1 (en) * | 2017-07-21 | 2019-01-24 | Beijing Biocytogen Co., Ltd | GENETICALLY MODIFIED IMMUNOGLOBULIN HEAVY CHAIN ANIMALS |
| CN107557367B (zh) * | 2017-09-08 | 2020-11-03 | 北京大学人民医院 | Ighg1基因突变体及其应用 |
| WO2021168577A1 (en) * | 2020-02-25 | 2021-09-02 | Symvivo Corporation | Gene delivery system |
| EP4256061A1 (en) * | 2020-12-01 | 2023-10-11 | ETH Zurich | Dna constructs comprising alternative promoters |
| CN120865355A (zh) * | 2024-04-29 | 2025-10-31 | 苏州系统医学研究所 | 一种工程化改造gpi锚定信号肽、其融合蛋白及应用 |
| CN118420709B (zh) * | 2024-04-29 | 2025-02-14 | 南京鼓楼医院 | 靶向cd73的肿瘤抗原肽、疫苗及其制备方法和应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| IE913929A1 (en) | 1990-11-13 | 1992-05-20 | Immunex Corp | Bifunctional selectable fusion genes |
| IL104570A0 (en) * | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5888809A (en) | 1997-05-01 | 1999-03-30 | Icos Corporation | Hamster EF-1α transcriptional regulatory DNA |
| ES2257303T3 (es) | 1999-07-12 | 2006-08-01 | Genentech, Inc. | Vectores de expresion y procedimientos. |
| AUPQ422399A0 (en) | 1999-11-24 | 1999-12-16 | University Of New South Wales, The | Method of screening transformed or transfected cells |
| IL136459A0 (en) | 2000-05-30 | 2001-06-14 | Galim Galil Immunology Ltd | Antibody library |
| US6919183B2 (en) | 2001-01-16 | 2005-07-19 | Regeneron Pharmaceuticals, Inc. | Isolating cells expressing secreted proteins |
| ATE311101T1 (de) | 2001-07-30 | 2005-12-15 | Smithkline Beecham Corp | Abbildung von transgenen markern |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| EP1477805A1 (de) | 2003-05-12 | 2004-11-17 | ProBioGen AG | Verfahren zur Zellselektion |
| US9128090B2 (en) | 2003-08-29 | 2015-09-08 | Janssen Biotech, Inc. | Rapid way to obtain high expression clones of mammalian cells using a methylcellulose and immunoprecipitation screening method |
| DE602004018349D1 (de) * | 2003-10-16 | 2009-01-22 | Claude Negrier | MODIFIZIERTE cDNA FÜR HOHE EXPRESSIONSNIVEAUS VON FAKTOR VIII UND DESSEN DERIVATEN |
| CA2559280A1 (en) * | 2004-03-15 | 2005-09-29 | Biogen Idec Ma Inc. | Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing |
| EP2027273B1 (en) * | 2006-05-17 | 2016-04-13 | F. Hoffmann-La Roche AG | Polypeptide producing cells |
-
2007
- 2007-05-15 EP EP07725230.2A patent/EP2027273B1/en not_active Not-in-force
- 2007-05-15 CN CN2007800108283A patent/CN101410525B/zh not_active Expired - Fee Related
- 2007-05-15 ES ES07725230.2T patent/ES2573027T3/es active Active
- 2007-05-15 JP JP2009510343A patent/JP4870208B2/ja not_active Expired - Fee Related
- 2007-05-15 AU AU2007251766A patent/AU2007251766B2/en not_active Ceased
- 2007-05-15 KR KR1020087027867A patent/KR101202498B1/ko not_active Expired - Fee Related
- 2007-05-15 WO PCT/EP2007/004313 patent/WO2007131774A1/en not_active Ceased
- 2007-05-15 ES ES10154684.4T patent/ES2543629T3/es active Active
- 2007-05-15 EP EP20100154684 patent/EP2208792B1/en not_active Not-in-force
- 2007-05-15 BR BRPI0712073A patent/BRPI0712073B8/pt not_active IP Right Cessation
- 2007-05-15 MX MX2008013514A patent/MX2008013514A/es active IP Right Grant
- 2007-05-15 CA CA2651478A patent/CA2651478C/en not_active Expired - Fee Related
- 2007-05-15 US US12/299,918 patent/US8354516B2/en not_active Expired - Fee Related
-
2008
- 2008-08-27 IL IL193721A patent/IL193721A/en active IP Right Grant
-
2012
- 2012-12-05 US US13/705,339 patent/US8541204B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2007251766A1 (en) | 2007-11-22 |
| EP2027273B1 (en) | 2016-04-13 |
| CN101410525A (zh) | 2009-04-15 |
| BRPI0712073A2 (pt) | 2012-03-06 |
| BRPI0712073B1 (pt) | 2019-11-12 |
| US20130109058A1 (en) | 2013-05-02 |
| EP2208792A3 (en) | 2010-10-27 |
| IL193721A (en) | 2014-09-30 |
| MX2008013514A (es) | 2008-10-28 |
| US20090311725A1 (en) | 2009-12-17 |
| WO2007131774A1 (en) | 2007-11-22 |
| US8354516B2 (en) | 2013-01-15 |
| JP2009537124A (ja) | 2009-10-29 |
| CA2651478C (en) | 2015-02-03 |
| EP2027273A1 (en) | 2009-02-25 |
| EP2208792B1 (en) | 2015-05-06 |
| EP2208792A2 (en) | 2010-07-21 |
| US8541204B2 (en) | 2013-09-24 |
| KR20090003327A (ko) | 2009-01-09 |
| ES2573027T3 (es) | 2016-06-03 |
| IL193721A0 (en) | 2011-08-01 |
| AU2007251766B2 (en) | 2013-06-20 |
| BRPI0712073B8 (pt) | 2021-05-25 |
| KR101202498B1 (ko) | 2012-11-16 |
| CA2651478A1 (en) | 2007-11-22 |
| JP4870208B2 (ja) | 2012-02-08 |
| CN101410525B (zh) | 2012-09-05 |
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