ES2546108T3 - Estabilidad de la producción masiva de metabolitos secundarios mediante cultivos sincronizados de células vegetales - Google Patents

Estabilidad de la producción masiva de metabolitos secundarios mediante cultivos sincronizados de células vegetales Download PDF

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Publication number
ES2546108T3
ES2546108T3 ES06757522.5T ES06757522T ES2546108T3 ES 2546108 T3 ES2546108 T3 ES 2546108T3 ES 06757522 T ES06757522 T ES 06757522T ES 2546108 T3 ES2546108 T3 ES 2546108T3
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cambium
stability
plant cell
plant
mass production
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Young Woo Jin
Eun Kyong Lee
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Unhwa Corp
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Unhwa Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para aislar una línea celular de una planta, en el que la línea celular aislada de la planta se caracteriza por que (i) procede de un cambium de la planta, (ii) es homogénea, (iii) no ha experimentado desdiferenciación para formar el callo, comprendiendo el método: (a) recoger un tejido que contiene el cambium de la planta; (b) cultivar el tejido que contiene el cambium, induciendo de esta forma una capa proliferada del cambium a partir del cambium; y (c) recoger las células que tienen continuidad meristemática del meristemo primario sin pasar por la desdiferenciación para obtener el callo aislando la capa de cambium.

Description

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E06757522
26-08-2015
y se extrajeron varias veces usando 4 ml de cloruro de metilo. La capa de disolvente orgánico separada se secó a vacío y el residuo se disolvió en 1 ml de metanol. El extracto disuelto se agitó igualmente un limpiador ultrasónico. Después, tras la centrifugación, se retiró el aglomerado (8.000 g X 5 min).
5 El medio (1-5 ml) separado de la célula se combinó con el mismo volumen de cloruro de metilo y se extrajo 3 veces tras una agitación completa. Después de evaporar a vacío y secar completamente, se disolvió en 0,5 ml de metanol de nuevo.
HPLC (cromatografía líquida de alto rendimiento, Shiseido, Japón) se usó para el análisis del contenido, y los
10 productos Sigma se usaron como sustancias patrón del paclitaxel. El envase Capcell (C18, MGII, 5 um, 3,0 mm x 250 mm, Shiseido, Japón) se mantuvo a 40 °C usando el horno, y agua y acetonitrilo (Burdick & Jackson, EE.UU.
50:50. v/v) se combinaron para la fase móvil y se añadieron gota a gota regularmente a la velocidad de 0,5 ml/min. Se usó el detector UV-VIS (227 nm, Shiseido, Japón).
15 Aplicabilidad industrial
En la presente invención, la adquisición de un clon monocelular, un meristemo primero que tiene continuidad meristemática del meristemo sin desdiferenciación, mediante la separación del procambium o del cambium simplemente a partir de la rama o el tallo dio como resultado una mayor productividad debido al menor tiempo de
20 duplicación que las líneas celulares de las técnicas anteriores. También permitió una productividad estable debido a menos cambios en el crecimiento celular y en el patrón de crecimiento durante el cultivo a largo plazo, y fue posible el escalado debido a la menor agregación y múltiples vacuolas de las líneas celulares. Estas líneas celulares permitieron la recuperación tras la crioconservación sin ninguna variación genética.
11

Claims (1)

  1. imagen1
ES06757522.5T 2005-10-31 2006-04-25 Estabilidad de la producción masiva de metabolitos secundarios mediante cultivos sincronizados de células vegetales Expired - Lifetime ES2546108T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20050103445 2005-10-31
KR20050103445 2005-10-31
PCT/KR2006/001544 WO2007052876A1 (en) 2005-10-31 2006-04-25 Stability of secondary metabolite mass production through syncronized plant cell cultures

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ES2546108T3 true ES2546108T3 (es) 2015-09-18

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US (2) US8017397B2 (es)
EP (2) EP2386626B1 (es)
JP (2) JP5211296B2 (es)
KR (2) KR101064519B1 (es)
CN (1) CN101297028B (es)
AU (1) AU2006309568B8 (es)
BR (1) BRPI0619656C1 (es)
CA (1) CA2627533C (es)
ES (1) ES2546108T3 (es)
PL (1) PL1951863T3 (es)
RU (1) RU2398874C2 (es)
WO (1) WO2007052876A1 (es)

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RU2500386C2 (ru) 2008-06-13 2013-12-10 Унхва Корпорейшн ПРОТИВОВОЗРАСТНАЯ ИЛИ АНТИОКСИДАНТНАЯ КОМПОЗИЦИЯ, СОДЕРЖАЩАЯ В КАЧЕСТВЕ АКТИВНОГО ИНГРЕДИЕНТА ЛИНИЮ РАСТИТЕЛЬНЫХ СТВОЛОВЫХ КЛЕТОК, ПОЛУЧЕННЫХ ИЗ КАМБИЯ Panax ginseng, ВКЛЮЧАЯ ЖЕНЬШЕНЬ ДИКОГО ТИПА И ЖЕНЬШЕНЬ КОРЕЙСКИЙ
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CN102459573A (zh) * 2009-05-28 2012-05-16 云火公司 茄科的形成层来源的植物干细胞及其分离培养方法
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KR101179171B1 (ko) 2008-08-14 2012-09-03 주식회사 운화 산삼 또는 인삼을 포함한 인삼류의 형성층 유래 식물줄기세포주를 유효성분으로 함유하는 간질환의 예방 또는 치료용 조성물
BRPI0919298A2 (pt) 2008-09-30 2019-09-24 Unhwa Corp composição para a prevenção ou o tratamento da aids que compreende a linhagem de células do tronco da planta derivada do câmbio da panax ginseng incluindo ginseng selvagem ou ginseng como ingrediente ativo.
WO2010095911A2 (ko) 2009-02-23 2010-08-26 주식회사 운화 산삼 또는 인삼을 포함한 인삼류의 형성층 유래 식물줄기세포주를 유효성분으로 함유하는 면역증강용 조성물

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US20080194025A1 (en) 2008-08-14
WO2007052876A1 (en) 2007-05-10
CA2627533C (en) 2015-09-08
AU2006309568B2 (en) 2012-03-15
KR20110004922A (ko) 2011-01-14
KR101064519B1 (ko) 2011-09-19
RU2398874C2 (ru) 2010-09-10
JP2009513157A (ja) 2009-04-02
AU2006309568A1 (en) 2007-05-10
EP2386626A1 (en) 2011-11-16
JP5211296B2 (ja) 2013-06-12
EP2386626B1 (en) 2016-10-26
EP1951863B1 (en) 2015-07-08
CN101297028A (zh) 2008-10-29
KR20080029971A (ko) 2008-04-03
US20110033903A1 (en) 2011-02-10
CA2627533A1 (en) 2007-05-10
BRPI0619656C1 (pt) 2011-12-20
JP6026209B2 (ja) 2016-11-16
EP1951863A1 (en) 2008-08-06
KR101020689B1 (ko) 2011-03-11
EP1951863A4 (en) 2009-05-20
PL1951863T3 (pl) 2015-12-31
RU2008121914A (ru) 2009-12-10
AU2006309568B8 (en) 2012-04-05
CN101297028B (zh) 2016-01-20
US8017397B2 (en) 2011-09-13
JP2013031451A (ja) 2013-02-14
BRPI0619656A2 (pt) 2011-10-11

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