ES2553331T3 - Nuevos genes marcadores seleccionables - Google Patents

Nuevos genes marcadores seleccionables Download PDF

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Publication number
ES2553331T3
ES2553331T3 ES07869044.3T ES07869044T ES2553331T3 ES 2553331 T3 ES2553331 T3 ES 2553331T3 ES 07869044 T ES07869044 T ES 07869044T ES 2553331 T3 ES2553331 T3 ES 2553331T3
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Prior art keywords
selectable marker
marker genes
new selectable
protein
new
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ES07869044.3T
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English (en)
Inventor
Justin M. Lira
Terry R. Wright
Sean M. Russell
Donald J. Merlo
Steven Robert Webb
Nicole L. Arnold
Andrew E. Robinson
Kelley A. Smith
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Corteva Agriscience LLC
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Dow AgroSciences LLC
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277Phosphinotricin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01183Phosphinothricin acetyltransferase (2.3.1.183)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Una célula vegetal transgénica que comprende un polinucleótido que codifica una proteína que tiene actividad fosfinotricina acetiltransferasa, en la que dicha proteína tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos de SEQ ID NO:2.

Description

19
ES07869044.3T 2006-12-07 2007-12-07 Nuevos genes marcadores seleccionables Active ES2553331T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87360206P 2006-12-07 2006-12-07
US873602P 2006-12-07
PCT/US2007/086813 WO2008070845A2 (en) 2006-12-07 2007-12-07 Novel selectable marker genes

Publications (1)

Publication Number Publication Date
ES2553331T3 true ES2553331T3 (es) 2015-12-07

Family

ID=39493101

Family Applications (2)

Application Number Title Priority Date Filing Date
ES13160763.2T Active ES2609848T3 (es) 2006-12-07 2007-12-07 Nuevos genes marcadores seleccionables
ES07869044.3T Active ES2553331T3 (es) 2006-12-07 2007-12-07 Nuevos genes marcadores seleccionables

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES13160763.2T Active ES2609848T3 (es) 2006-12-07 2007-12-07 Nuevos genes marcadores seleccionables

Country Status (17)

Country Link
US (3) US10752913B2 (es)
EP (2) EP2094831B1 (es)
JP (1) JP5563825B2 (es)
CN (3) CN106987561A (es)
AR (2) AR064200A1 (es)
AU (1) AU2007329208B2 (es)
BR (1) BRPI0720226B8 (es)
CA (1) CA2671365C (es)
DK (2) DK2610334T3 (es)
ES (2) ES2609848T3 (es)
HU (2) HUE026199T2 (es)
MX (1) MX2009006093A (es)
NZ (1) NZ577363A (es)
PL (1) PL2610334T3 (es)
PT (1) PT2610334T (es)
SI (1) SI2610334T1 (es)
WO (1) WO2008070845A2 (es)

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CA2727430C (en) * 2008-06-11 2018-10-02 Dow Agrosciences Llc Constructs for expressing herbicide tolerance genes, related plants, and related trait combinations
WO2010046422A2 (en) * 2008-10-22 2010-04-29 Basf Se Use of auxin type herbicides on cultivated plants
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US8598413B2 (en) * 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
ES2719599T3 (es) 2009-08-19 2019-07-11 Dow Agrosciences Llc Evento DAS-40278-9 que incorpora add-1, líneas transgénicas de maíz relacionadas e identificación evento-específico de las mismas
WO2011075588A1 (en) 2009-12-16 2011-06-23 Dow Agrosciences Llc COMBINED USE OF CRY1Ca AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT
PL2512226T3 (pl) * 2009-12-17 2019-10-31 Pioneer Hi Bred Int Modyfikacja DP-004114-3 kukurydzy i sposoby jej wykrywania
WO2013010094A1 (en) * 2011-07-13 2013-01-17 Dow Agrosciences Llc Stacked herbicide tolerance event 8264.42.32.1, related transgenic soybean lines, and detection thereof
CN102559717B (zh) * 2012-01-05 2013-03-06 华中农业大学 一种抗寒的草铵膦抗性基因及应用
JP6242345B2 (ja) 2012-02-01 2017-12-06 ダウ アグロサイエンシィズ エルエルシー グリホサート抵抗性植物および関連する方法
CN103320433A (zh) * 2013-06-06 2013-09-25 中国科学院广州生物医药与健康研究院 一种用于高效构建无抗性标记重组分枝杆菌的抗性表达盒
AP2016009164A0 (en) 2013-10-04 2016-04-30 Dow Agrosciences Llc Zea mays metallothionein-like regulatory elements and uses thereof
BR102014025499A2 (pt) 2013-10-15 2015-09-29 Dow Agrosciences Llc elementos regulatórios de zea mays e uso dos mesmos
UA121743C2 (uk) 2013-12-10 2020-07-27 Дау Аґросаєнсиз Елелсі Синергетична боротьба з бур'янами при використанні комбінацій гербіцидів, які включають 2,4-d-холін і глуфосинат
WO2015094884A1 (en) 2013-12-20 2015-06-25 Dow Agrosciences Llc Synergistic herbicidal weed control
AR100785A1 (es) 2014-06-09 2016-11-02 Dow Agrosciences Llc Control herbicida de maleza a partir de combinaciones de fluroxipir e inhibidores de als
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
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BR102016012010A2 (pt) 2015-05-29 2020-03-24 Dow Agrosciences Llc Molécula de ácido nucleico, de ácido ribonucleico (rna) e de ácido ribonucleico de filamento duplo (dsrna), usos de célula, planta e semente, produto primário, bem como métodos para controlar uma população de pragas coleópteras e/ou hemípteras, para melhorar o rendimento de uma cultura, e para produzir uma célula vegetal transgênica e uma planta transgênica
WO2018136547A1 (en) * 2017-01-17 2018-07-26 Noble Research Institute, Llc Dna-free genome editing and selection methods in plants
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Also Published As

Publication number Publication date
EP2094831A2 (en) 2009-09-02
AU2007329208B2 (en) 2014-06-12
CN101600790B (zh) 2020-08-04
WO2008070845A3 (en) 2008-11-27
CN101600790A (zh) 2009-12-09
US20110107455A1 (en) 2011-05-05
JP2010512153A (ja) 2010-04-22
AR109558A2 (es) 2018-12-19
AR064200A1 (es) 2009-03-18
ES2609848T3 (es) 2017-04-24
SI2610334T1 (sl) 2017-04-26
CA2671365C (en) 2020-07-21
HUE026199T2 (en) 2016-05-30
EP2610334A1 (en) 2013-07-03
NZ577363A (en) 2012-02-24
US10752913B2 (en) 2020-08-25
AU2007329208A1 (en) 2008-06-12
PL2610334T3 (pl) 2017-05-31
BRPI0720226A2 (pt) 2013-12-24
US20200385751A1 (en) 2020-12-10
BRPI0720226B8 (pt) 2022-06-28
DK2610334T3 (en) 2017-01-30
CN111944759A (zh) 2020-11-17
PT2610334T (pt) 2017-01-30
BRPI0720226B1 (pt) 2017-08-22
DK2094831T3 (en) 2015-11-23
WO2008070845A2 (en) 2008-06-12
HUE031454T2 (en) 2017-07-28
EP2094831A4 (en) 2010-06-23
JP5563825B2 (ja) 2014-07-30
MX2009006093A (es) 2009-06-17
CA2671365A1 (en) 2008-06-12
EP2610334B1 (en) 2016-10-26
US20200385752A1 (en) 2020-12-10
EP2094831B1 (en) 2015-09-16
CN106987561A (zh) 2017-07-28

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