BRPI0612288A2 - amilase modificada de pseudomonas saccharophilia - Google Patents

amilase modificada de pseudomonas saccharophilia

Info

Publication number
BRPI0612288A2
BRPI0612288A2 BRPI0612288-4A BRPI0612288A BRPI0612288A2 BR PI0612288 A2 BRPI0612288 A2 BR PI0612288A2 BR PI0612288 A BRPI0612288 A BR PI0612288A BR PI0612288 A2 BRPI0612288 A2 BR PI0612288A2
Authority
BR
Brazil
Prior art keywords
polypeptide
amino acid
acid mutation
amylase
pseudomonas saccharophilia
Prior art date
Application number
BRPI0612288-4A
Other languages
English (en)
Inventor
Casper Tune Berg
Patrick Maria Franciscus Derkx
Carol Fioresi
Gijsbert Gerritse
Kellet-Smith Anja Hemmingen
Karsten Kragh
Wei Liu
Andrew Shaw
Bo Spange Sorensen
Thoudahl Charlotte Refdahl
Original Assignee
Danisco
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco, Genencor Int filed Critical Danisco
Publication of BRPI0612288A2 publication Critical patent/BRPI0612288A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Cereal-Derived Products (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

AMILASE MODIFICADA DE PSEUDONONAS SACCHIAROPHILIA A presente invenção descreve um polipeptídeo variante PS4 derivável de um polipeptídeo de origem com atividade amílase selecionado do grupo consistindo de: (a) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 e 334; (b) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 e 334; (o) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 e 334; e (d) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 e 334; com referência à numeração de posição de uma seqúência exoamilase de Pseudomonas saccharophilia mostrada como SEQ ID NO: 1, usos de tal polipeptídeo como um aditivo de ração ou alimentício e ácidos nuclélcos que o codificam.
BRPI0612288-4A 2005-07-07 2006-07-07 amilase modificada de pseudomonas saccharophilia BRPI0612288A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69730205P 2005-07-07 2005-07-07
PCT/GB2006/002513 WO2007007053A1 (en) 2005-07-07 2006-07-07 Modified amylase from pseudomonas saccharophilia

Publications (1)

Publication Number Publication Date
BRPI0612288A2 true BRPI0612288A2 (pt) 2009-01-27

Family

ID=37331082

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0612288-4A BRPI0612288A2 (pt) 2005-07-07 2006-07-07 amilase modificada de pseudomonas saccharophilia

Country Status (11)

Country Link
US (2) US20070141693A1 (pt)
EP (1) EP1907538A1 (pt)
JP (1) JP2008544751A (pt)
KR (1) KR20080023746A (pt)
CN (1) CN101238210A (pt)
AU (1) AU2006268418A1 (pt)
BR (1) BRPI0612288A2 (pt)
CA (1) CA2614274A1 (pt)
MX (1) MX2008000374A (pt)
RU (1) RU2008104637A (pt)
WO (1) WO2007007053A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2342287T3 (es) * 2003-06-13 2010-07-05 Danisco A/S Polipeptidos de pseudomonas variantes que presentan una actividad de exoamilasa no maltogena y su utilizacion en la preparacion de productos alimenticios.
US8143048B2 (en) * 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
US7371552B2 (en) * 2003-07-07 2008-05-13 Danisco A/S Polypeptide
EP2295557B1 (en) * 2004-07-07 2017-09-06 DuPont Nutrition Biosciences ApS Exoamylase variants
KR20080023746A (ko) * 2005-07-07 2008-03-14 대니스코 에이/에스 슈도모나스 사카로필리아에 의해 변형된 아밀라아제
US8030050B2 (en) * 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species
ES2644745T3 (es) * 2006-06-19 2017-11-30 Dupont Nutrition Biosciences Aps Polipéptido
US7666637B2 (en) * 2006-09-05 2010-02-23 Xuan Nghinh Nguyen Integrated process for separation of lignocellulosic components to fermentable sugars for production of ethanol and chemicals
CN101918545A (zh) * 2008-01-02 2010-12-15 丹尼斯科美国公司 应用嗜糖假单胞菌g4-淀粉酶和其变体获得乙醇的无葡糖淀粉酶的方法
JP2010148488A (ja) * 2008-12-26 2010-07-08 Biomaterial In Tokyo Co Ltd カンジダ・グラブラータ（Ｃ．ｇｌａｂｒａｔａ）を用いるエタノール製造方法
EP2417255A2 (en) * 2009-04-10 2012-02-15 Danisco US Inc. Production of maltotetraose syrup using a pseudomonas saccharophila maltotetraohyfrolase variant
EP2432875B1 (en) 2009-05-19 2016-09-07 DuPont Nutrition Biosciences ApS Amylase polypeptides
DK2552945T3 (en) 2010-03-29 2018-07-16 Dupont Nutrition Biosci Aps POLYPEPTIDES WITH TRANSGALACTOSYLATION ACTIVITY
CA2852603C (en) 2011-10-17 2025-10-07 Novozymes A/S VARIANTS OF ALPHA-AMYLASE AND POLYNUCLEOTIDES CODING THEM
MX355356B (es) 2011-10-17 2018-04-17 Novozymes As Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
BR112014030009B1 (pt) 2012-06-08 2022-11-08 Dupont Nutrition Biosciences Aps. Método para a produção de um produto alimentar compreendendo galacto- oligossacarídeos
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2015086027A1 (en) 2013-12-09 2015-06-18 Carlsberg A/S Stable haze for beverages
AR098708A1 (es) 2013-12-11 2016-06-08 Dupont Nutrition Biosci Aps Método para preparar un producto lácteo con un contenido estable de galacto-oligosacárido/s
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
WO2016071504A1 (en) 2014-11-07 2016-05-12 Dupont Nutrition Biosciences Aps Recombinant host cell expressing beta-galactosidase and/or transgalactosylating activity deficient in mannanase, cellulase and pectinase
FI3607097T3 (fi) 2017-04-07 2023-09-11 Dupont Nutrition Biosci Aps BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA
EP4071242A1 (en) * 2021-04-06 2022-10-12 DuPont Nutrition Biosciences ApS Amylase polypeptides with improved properties

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CA1171374A (en) * 1981-04-13 1984-07-24 Takeda Chemical Industries, Ltd. Pseudo-aminosugars, their production and use
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JP2660836B2 (ja) * 1987-07-08 1997-10-08 株式会社 林原生物化学研究所 マルトテトラオース生成アミラーゼ活性を有するポリペプチドとその用途
DE4017595A1 (de) * 1990-05-31 1991-12-05 Consortium Elektrochem Ind Maltopentaose produzierende amylasen
JP3533239B2 (ja) * 1994-03-01 2004-05-31 株式会社林原生物化学研究所 マルトヘキサオース・マルトヘプタオース生成アミラーゼとその製造方法並びに用途
JP4047379B2 (ja) * 1995-02-03 2008-02-13 ノボザイムス アクティーゼルスカブ 予め決められた性質を有するα−アミラーゼ変異体のデザイン方法
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
DE69942995D1 (de) * 1998-02-27 2011-01-13 Novozymes As Maltogene alpha-amylase varianten
ES2241267T3 (es) * 1998-04-01 2005-10-16 Danisco A/S Exoamilasas no maltogenicas y su utilizacion en el retraso de la retrogradacion del almidon.
US20030134395A1 (en) * 2001-12-19 2003-07-17 Shetty Jayarama K. Process for hydrolyzing starch without pH adjustment
ES2342287T3 (es) * 2003-06-13 2010-07-05 Danisco A/S Polipeptidos de pseudomonas variantes que presentan una actividad de exoamilasa no maltogena y su utilizacion en la preparacion de productos alimenticios.
US7371552B2 (en) * 2003-07-07 2008-05-13 Danisco A/S Polypeptide
US8143048B2 (en) * 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
US20060073583A1 (en) * 2004-09-22 2006-04-06 Kragh Karsten M Polypeptide
US20060008888A1 (en) * 2004-07-07 2006-01-12 Kragh Karsten M Polypeptide
US20060008890A1 (en) * 2004-07-07 2006-01-12 Kragh Karsten M Polypeptide
US20060018997A1 (en) * 2004-07-07 2006-01-26 Kragh Karsten M Polypeptide
KR20080023746A (ko) * 2005-07-07 2008-03-14 대니스코 에이/에스 슈도모나스 사카로필리아에 의해 변형된 아밀라아제
US8030050B2 (en) * 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species

Also Published As

Publication number Publication date
AU2006268418A1 (en) 2007-01-18
WO2007007053A1 (en) 2007-01-18
MX2008000374A (es) 2008-03-07
US20080292747A1 (en) 2008-11-27
KR20080023746A (ko) 2008-03-14
EP1907538A1 (en) 2008-04-09
CN101238210A (zh) 2008-08-06
JP2008544751A (ja) 2008-12-11
RU2008104637A (ru) 2009-08-20
US20070141693A1 (en) 2007-06-21
CA2614274A1 (en) 2007-01-18

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: NAO APRESENTADA A GUIA DE CUMPRIMENTO DE EXIGENCIA. REFERENTE A 6A ANUIDADE.