ES2571355T3 - Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas - Google Patents

Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas

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Publication number
ES2571355T3
ES2571355T3 ES03813286T ES03813286T ES2571355T3 ES 2571355 T3 ES2571355 T3 ES 2571355T3 ES 03813286 T ES03813286 T ES 03813286T ES 03813286 T ES03813286 T ES 03813286T ES 2571355 T3 ES2571355 T3 ES 2571355T3
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ES
Spain
Prior art keywords
xenocontaminants
culture system
embryonic stem
human embryonic
cells
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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ES03813286T
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English (en)
Inventor
Michal Amit
Joseph Itskovitz-Eldor
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Technion Research and Development Foundation Ltd
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Technion Research and Development Foundation Ltd
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0602—Vertebrate cells
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    • C12N2500/05—Inorganic components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un sistema de cultivo sin células alimentadoras ni xenocontaminantes que comprende una matriz y un medio de cultivo tisular que comprende sustitutivo del suero, TLFß1 y bFGF, pudiendo mantener el sistema de cultivo sin células alimentadoras ni xenocontaminantes, células madre embrionarias humanas cultivadas en su interior, en un estado proliferativo, pluripotente e indiferenciado.
ES03813286T 2002-12-16 2003-12-07 Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas Expired - Lifetime ES2571355T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43361902P 2002-12-16 2002-12-16
PCT/IL2003/001030 WO2004055155A2 (en) 2002-12-16 2003-12-07 Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same

Publications (1)

Publication Number Publication Date
ES2571355T3 true ES2571355T3 (es) 2016-05-24

Family

ID=32595220

Family Applications (2)

Application Number Title Priority Date Filing Date
ES11190776T Expired - Lifetime ES2705683T3 (es) 2002-12-16 2003-12-07 Medio de cultivo de células madre pluripotentes
ES03813286T Expired - Lifetime ES2571355T3 (es) 2002-12-16 2003-12-07 Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES11190776T Expired - Lifetime ES2705683T3 (es) 2002-12-16 2003-12-07 Medio de cultivo de células madre pluripotentes

Country Status (14)

Country Link
US (8) US7592175B2 (es)
EP (2) EP2457999B1 (es)
JP (1) JP4613069B2 (es)
KR (1) KR101156745B1 (es)
CN (1) CN100549163C (es)
AU (2) AU2003303071B2 (es)
CA (1) CA2508880C (es)
DK (2) DK1572984T3 (es)
ES (2) ES2705683T3 (es)
HU (1) HUE028026T2 (es)
IL (2) IL168984A (es)
PT (2) PT2457999T (es)
SI (1) SI1572984T1 (es)
WO (1) WO2004055155A2 (es)

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AU2003303071B2 (en) 2002-12-16 2009-04-23 Technion Research & Development Foundation Ltd. Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
WO2005065354A2 (en) * 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
TWI334443B (en) 2003-12-31 2010-12-11 Ind Tech Res Inst Method of single cell culture of undifferentiated human embryonic stem cells
JP5128946B2 (ja) * 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
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JP2006115767A (ja) * 2004-10-21 2006-05-11 Kyoto Univ フィーダー細胞の不在下で精原幹細胞を増殖させる方法
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JP2009500297A (ja) 2005-06-08 2009-01-08 セントカー・インコーポレーテツド 眼の変性についての細胞療法
JP5560391B2 (ja) 2005-06-22 2014-07-23 アステリアス バイオセラピューティクス インコーポレイテッド ヒト胚性幹細胞の懸濁培養法
US9101590B2 (en) * 2005-07-29 2015-08-11 Yale University Defined culture conditions of human embryonic stem cells
US8062890B2 (en) 2005-08-15 2011-11-22 Wisconsin Alumni Research Foundation Defined surfaces of self-assembled monolayers and stem cells
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