ES2614352T3 - Método para la detección de moduladores de la interacción de GPIb-trombina - Google Patents

Método para la detección de moduladores de la interacción de GPIb-trombina Download PDF

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Publication number
ES2614352T3
ES2614352T3 ES14158716.2T ES14158716T ES2614352T3 ES 2614352 T3 ES2614352 T3 ES 2614352T3 ES 14158716 T ES14158716 T ES 14158716T ES 2614352 T3 ES2614352 T3 ES 2614352T3
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ES
Spain
Prior art keywords
thrombin
gpib
modulators
interaction
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES14158716.2T
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English (en)
Inventor
Juergen Patzke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Products GmbH
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Siemens Healthcare Diagnostics Products GmbH
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Publication date
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Application granted granted Critical
Publication of ES2614352T3 publication Critical patent/ES2614352T3/es
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/755—Factors VIII, e.g. factor VIII C [AHF], factor VIII Ag [VWF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método para la detección de moduladores del interacción de GPIb-trombina en una muestra, en el que la muestra es puesta en contacto con proteína GPIb- aislada y con trombina aislada y se determina la formación de complejo entre la proteína GPIbα y trombina, caracterizado porque la proteína GPIbα ha hecho mutación y, comparada con la secuencia tipo silvestre de la proteína GPIbα humana, contiene por lo menos los residuos de aminoácidos 1-268 y en por lo menos una de las posiciones 233, 235, 237 y 239 exhibe una sustitución Xaa (SEQ ID NO: 1).

Description

imagen1
imagen2
imagen3
imagen4

Claims (1)

  1. imagen1
ES14158716.2T 2014-03-11 2014-03-11 Método para la detección de moduladores de la interacción de GPIb-trombina Active ES2614352T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP14158716.2A EP2919013B1 (de) 2014-03-11 2014-03-11 Verfahren zur Detektion von Modulatoren der GPIb-Thrombin-Interaktion

Publications (1)

Publication Number Publication Date
ES2614352T3 true ES2614352T3 (es) 2017-05-30

Family

ID=50272363

Family Applications (1)

Application Number Title Priority Date Filing Date
ES14158716.2T Active ES2614352T3 (es) 2014-03-11 2014-03-11 Método para la detección de moduladores de la interacción de GPIb-trombina

Country Status (5)

Country Link
US (2) US10281480B2 (es)
EP (1) EP2919013B1 (es)
JP (1) JP6418986B2 (es)
CN (1) CN104914246B (es)
ES (1) ES2614352T3 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11746140B2 (en) 2018-02-20 2023-09-05 F. Hoffman-La Roche Ag Composition comprising recombinant GPlbα receptor protein

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69232161T2 (de) 1991-05-22 2002-05-16 Dade Behring Marburg Gmbh Bestimmungsmethoden unter Verwendung von induzierter Lumineszenz
WO1995006877A1 (en) 1993-09-03 1995-03-09 Behringwerke Ag Fluorescent oxygen channeling immunoassays
US5486361A (en) 1993-10-25 1996-01-23 The United States Of America As Represented By The Department Of Health And Human Services Hybridomas and monoclonal antibodies that speifically bind to GPIB on platelets and inhibit the binding of thrombin to platelets
AR035779A1 (es) * 2001-02-06 2004-07-14 Genetics Inst Llc Polipeptidos de fusion derivados de glicoproteina ib alfa de plaqueta y metodos de uso de los mismos
JP2007537710A (ja) * 2003-06-11 2007-12-27 ワイス 血小板糖タンパク質ＩＢα改変体融合ポリペプチドおよびその使用法
DE102007031708A1 (de) 2007-07-06 2009-01-08 Dade Behring Marburg Gmbh Bestimmung der von Willebrand Faktor-Aktivität in Abwesenheit von Ristocetin
EP2193373A4 (en) * 2007-08-23 2010-07-28 Bloodct Of Wisconsin Inc METHODS AND KITS FOR MEASURING VON WILLEBRAND FACTOR
IL209921A0 (en) * 2010-12-09 2011-02-28 Omrix Biopharmaceuticals Immunoassay for thrombin detection
CN102279273A (zh) * 2011-07-19 2011-12-14 苏州大学 一种血管性血友病因子瑞斯托霉素辅因子酶联免疫试剂盒
CN102993307A (zh) * 2011-09-09 2013-03-27 苏州苏大赛尔免疫生物技术有限公司 抗人血小板膜糖蛋白Ibα嵌合抗体
EP2637024A1 (de) 2012-03-05 2013-09-11 Siemens Healthcare Diagnostics Products GmbH Screeningverfahren zum Auffinden von Proben mit einer Störung der Funktionalität der GPIb-Von Willebrand Faktor-Interaktion

Also Published As

Publication number Publication date
CN104914246A (zh) 2015-09-16
US20150260736A1 (en) 2015-09-17
US10281480B2 (en) 2019-05-07
EP2919013B1 (de) 2016-11-02
EP2919013A1 (de) 2015-09-16
US20190227084A1 (en) 2019-07-25
JP2015172577A (ja) 2015-10-01
JP6418986B2 (ja) 2018-11-07
CN104914246B (zh) 2018-06-01

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