CL2016003096A1 - Métodos para cosechar cultivos de células de mamífero - Google Patents

Métodos para cosechar cultivos de células de mamífero

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Publication number
CL2016003096A1
CL2016003096A1 CL2016003096A CL2016003096A CL2016003096A1 CL 2016003096 A1 CL2016003096 A1 CL 2016003096A1 CL 2016003096 A CL2016003096 A CL 2016003096A CL 2016003096 A CL2016003096 A CL 2016003096A CL 2016003096 A1 CL2016003096 A1 CL 2016003096A1
Authority
CL
Chile
Prior art keywords
methods
mammalian cell
cell cultures
harvesting mammalian
harvesting
Prior art date
Application number
CL2016003096A
Other languages
English (en)
Inventor
Jonathan Lull
Tharmala Tharmalingam
Chetan Goudar
Sean Cole
Henry Lin
Nicole Sabo
Original Assignee
Amgen Inc
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53433304&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2016003096(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of CL2016003096A1 publication Critical patent/CL2016003096A1/es

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Computer Hardware Design (AREA)
  • Water Supply & Treatment (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

<p>LA INVENCIÓN PROPORCIONA MÉTODOS Y MATERIALES PARA CULTIVAR CÉLULAS DE MAMÍFERO Y COSECHAR PROTEÍNAS RECOMBINANTES.</p>
CL2016003096A 2014-06-04 2016-12-01 Métodos para cosechar cultivos de células de mamífero CL2016003096A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462007588P 2014-06-04 2014-06-04

Publications (1)

Publication Number Publication Date
CL2016003096A1 true CL2016003096A1 (es) 2017-11-03

Family

ID=53433304

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2016003096A CL2016003096A1 (es) 2014-06-04 2016-12-01 Métodos para cosechar cultivos de células de mamífero
CL2019001641A CL2019001641A1 (es) 2014-06-04 2019-06-14 Sistema de filtro unitario individual. (divisional solicitud 201603096).

Family Applications After (1)

Application Number Title Priority Date Filing Date
CL2019001641A CL2019001641A1 (es) 2014-06-04 2019-06-14 Sistema de filtro unitario individual. (divisional solicitud 201603096).

Country Status (25)

Country Link
US (3) US11384378B2 (es)
EP (4) EP3521440B1 (es)
JP (3) JP6695814B2 (es)
KR (2) KR102391259B1 (es)
CN (1) CN106687575B (es)
AU (5) AU2015269365A1 (es)
BR (2) BR112016028285B1 (es)
CA (2) CA3195249A1 (es)
CL (2) CL2016003096A1 (es)
CY (1) CY1121217T1 (es)
DK (2) DK3926051T3 (es)
EA (2) EA202192126A3 (es)
ES (3) ES2909630T3 (es)
FI (1) FI3926051T3 (es)
HU (2) HUE042277T2 (es)
IL (3) IL249150B (es)
LT (2) LT3152317T (es)
MX (3) MX384212B (es)
PL (2) PL3152317T3 (es)
PT (2) PT3152317T (es)
SG (2) SG11201610143VA (es)
SI (2) SI3926051T1 (es)
TR (1) TR201900776T4 (es)
WO (1) WO2015188009A1 (es)
ZA (1) ZA201608303B (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105143443A (zh) 2013-02-22 2015-12-09 建新公司 微载体灌注培养方法及其用途
CN105209603A (zh) 2013-02-22 2015-12-30 建新公司 微载体灌注培养方法及其用途
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
KR102769649B1 (ko) * 2014-12-22 2025-02-19 젠자임 코포레이션 포유류 세포의 배양 방법
JOP20160086B1 (ar) 2015-05-08 2021-08-17 2 Katholieke Univ Leuven Ku Leuven Research And Development مشتقات اندول مستبدلة احاديا او ثنائيا بصفتها مانعات للتكاثر الفيروسي لحمى الفنك
CA3032099A1 (en) * 2016-07-25 2018-02-01 Repligen Corporation Alternating tangential flow rapid harvesting
JP7043125B2 (ja) * 2016-10-28 2022-03-29 旭化成メディカル株式会社 連続培養における有用物質の回収方法
GB201622343D0 (en) 2016-12-29 2017-02-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
CN107964037A (zh) * 2017-05-16 2018-04-27 浙江海隆生物科技有限公司 基于cho细胞表达系统的重组猪瘟e2蛋白的纯化方法及应用
CN110945593A (zh) * 2017-06-16 2020-03-31 通用电气健康护理生物科学股份公司 用于预测生物反应器中的过程的产出和对生物反应器中的过程建模的方法
AU2018404665A1 (en) 2017-08-31 2020-02-27 Massachusetts Institute Of Technology Hollow fibre membrane filtration systems and methods for manufacturing biologically-produced products
WO2019071076A1 (en) * 2017-10-06 2019-04-11 Lonza Ltd. AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN
EP3700505A4 (en) * 2017-10-26 2021-01-06 Repligen Corporation MICRO-ALTERNATIVE TANGENTIAL FLOW INFUSION FILTER, PROCESSING CONTAINER, AND METHODS OF USE THEREOF
IL274776B2 (en) 2017-11-20 2025-02-01 Lonza Ag Process and system for growing cell cultures while preventing lactate accumulation
DK3770266T3 (da) * 2018-03-19 2026-07-20 Fujifilm Corp Produktfremstillingsfremgangsmåde
GB201810772D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
IL279782B2 (en) * 2018-07-03 2026-05-01 Bristol Myers Squibb Co Methods for producing recombinant proteins
WO2020088180A1 (en) * 2018-11-02 2020-05-07 Wuxi Biologics (Shanghai) Co., Ltd. Cell culture process by intensified perfusion with continuous harvest and without cell bleeding
JP7560459B2 (ja) 2018-12-21 2024-10-02 ジェネンテック， インコーポレイテッド アポトーシスに耐性のある細胞株を使用してポリペプチドを生成する方法
JP2022516516A (ja) * 2018-12-31 2022-02-28 レプリゲン・コーポレーション 疎水性中空繊維による哺乳類細胞培養物の灌流および浄化用フィルター
JP7340313B2 (ja) * 2019-08-13 2023-09-07 レプリゲン・コーポレーション 固形分を多く含む細胞培養物の自動清澄化のための制御システムおよび方法
WO2021097281A1 (en) 2019-11-15 2021-05-20 Lonza Ltd Process and system for producing an inoculum
PH12022551160A1 (en) 2019-11-15 2023-09-25 Janssen Pharmaceuticals Inc Treatment and prevention of dengue disease
CA3158269A1 (en) * 2020-01-10 2021-07-15 Yonatan LEVINSON Filter apparatus and method for purifying biological processes and cell populations
TWI777364B (zh) * 2020-01-15 2022-09-11 大陸商上海藥明生物技術有限公司 連續收穫由培養的細胞生產的生物物質的設備和方法
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
EP3985097A1 (en) * 2020-10-14 2022-04-20 Sartorius Stedim Biotech GmbH A method and system for configuring and/or setup of a downstream process for processing a biomass
EP4228794A1 (en) * 2020-10-15 2023-08-23 AGC Biologics, Inc. Continuous high cell-density culture with dual-vessel tangential flow filtration
US20220119759A1 (en) * 2020-10-19 2022-04-21 Emd Millipore Corporation Cascade Tangential Flow Filtration Systems for Perfusion of Cell Culture
US20220268785A1 (en) * 2021-02-19 2022-08-25 Northwestern University Facile sample preparation for quantitative single-cell proteomics
JP2024507578A (ja) * 2021-02-25 2024-02-20 セキュアセル アクチェンゲゼルシャフト バイオテクノロジー用途のための流体サンプリングシステム、その操作方法および使用
CA3222382A1 (en) * 2021-06-09 2022-12-15 Amgen Inc. Assessing packed cell volume for cell cultures
FR3126713A1 (fr) * 2021-09-08 2023-03-10 Erypharm Procédé de production de cellules de culture à haute densité
CN114317392A (zh) * 2021-12-27 2022-04-12 无锡药明生物技术股份有限公司 优化细胞培养的方法
CN119630774A (zh) * 2022-03-01 2025-03-14 拜奥莎娜制药有限公司 具有过程可追溯性的生物物质的连续制备
US20250250524A1 (en) * 2022-04-14 2025-08-07 Terasaki Institute For Biomedical Innovation Systems and methods for recycling growth factors and other components
WO2023205075A1 (en) * 2022-04-22 2023-10-26 Coopersurgical, Inc. Methods of culturing umbilical cord mesenchymal stem cells
AR130495A1 (es) 2022-09-16 2024-12-11 Amgen Inc Un método para recoger productos a partir de cultivos en perfusión
WO2024173685A1 (en) * 2023-02-15 2024-08-22 The Board Of Trustees Of The Leland Stanford Junior University Metabolic selection for glycogen-storing cells in vitro
EP4722338A1 (en) 2023-05-31 2026-04-08 FUJIFILM Corporation Cell culture method and product producing method
EP4731319A2 (en) 2023-06-20 2026-04-29 Amgen Inc. Methods for chromatography and chromatography medium reuse
WO2025184296A1 (en) * 2024-02-29 2025-09-04 Nature's Toolbox, Inc. In vitro rna production and purification system and methods of use thereof

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
US5223428A (en) 1982-12-14 1993-06-29 Baxter International Inc. Method for in vitro culture of mammalian cells
AU588819B2 (en) 1984-10-29 1989-09-28 Immunex Corporation Cloning of human granulocyte-macrophage colony stimulating factor gene
US4968607A (en) 1987-11-25 1990-11-06 Immunex Corporation Interleukin-1 receptors
US5075222A (en) 1988-05-27 1991-12-24 Synergen, Inc. Interleukin-1 inhibitors
JP2507552B2 (ja) * 1988-08-29 1996-06-12 株式会社ニッショー 細胞培養産生物の取出しおよび培地の交換方法
WO1990005183A1 (en) 1988-10-31 1990-05-17 Immunex Corporation Interleukin-4 receptors
US4937196A (en) 1989-08-18 1990-06-26 Brunswick Corporation Membrane bioreactor system
US5395760A (en) 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
DE59010908D1 (de) 1989-09-12 2000-08-10 Hoffmann La Roche TNF-bindende Proteine
US6204363B1 (en) 1989-10-16 2001-03-20 Amgen Inc. Stem cell factor
US5149792A (en) 1989-12-19 1992-09-22 Amgen Inc. Platelet-derived growth factor B chain analogs
US5272064A (en) 1989-12-19 1993-12-21 Amgen Inc. DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof
US5378612A (en) 1990-05-11 1995-01-03 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Culture medium for production of recombinant protein
WO1991018982A1 (en) 1990-06-05 1991-12-12 Immunex Corporation Type ii interleukin-1 receptors
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
PT672141E (pt) 1992-10-23 2003-09-30 Immunex Corp Metodos de preparacao de proteinas oligomericas soluveis
US5554512A (en) 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
US5981713A (en) 1994-10-13 1999-11-09 Applied Research Systems Ars Holding N.V. Antibodies to intereleukin-1 antagonists
US5721121A (en) 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
CA2225378C (en) 1995-06-29 2012-04-17 Immunex Corporation Cytokine that induces apoptosis
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US6096728A (en) 1996-02-09 2000-08-01 Amgen Inc. Composition and method for treating inflammatory diseases
US6271349B1 (en) 1996-12-23 2001-08-07 Immunex Corporation Receptor activator of NF-κB
US6235883B1 (en) 1997-05-05 2001-05-22 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US6337072B1 (en) 1998-04-03 2002-01-08 Hyseq, Inc. Interleukin-1 receptor antagonist and recombinant production thereof
US6068775A (en) * 1998-04-13 2000-05-30 Circe Biomedical, Inc. Removal of agent from cell suspension
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
AU1920301A (en) 1999-11-17 2001-05-30 Immunex Corporation Receptor activator of nf-kappa b
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
TWI314933B (en) 2000-05-26 2009-09-21 Bristol Myers Squibb Compan Soluble ctla4 mutant molecules and uses thereof
AU2001291653A1 (en) 2000-10-02 2002-04-15 Novo-Nordisk A/S Industrial-scale serum-free production of recombinant factor vii in mammalian cells
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
US7198940B2 (en) 2000-10-25 2007-04-03 Shot Hardware Optimization Technology, Inc. Bioreactor apparatus and cell culturing system
TR201809678T4 (tr) * 2001-06-26 2018-07-23 Amgen Fremont Inc Opgl ye karşi antikorlar.
DK1520008T3 (da) 2002-07-09 2012-10-15 Baxter Int Medium frit for dyreprotein til dyrkning af celler
MXPA05002514A (es) * 2002-09-06 2005-05-27 Amgen Inc Anticuerpo monoclonal anti-il-1r1 humano terapeutico.
EP1575998A4 (en) * 2002-12-23 2007-07-25 Bristol Myers Squibb Co PROCESS FOR CULTIVATING MAMMALIAN CELLS FOR PROTEIN PRODUCTION
DE10312765A1 (de) 2003-03-21 2004-09-30 Bayer Technology Services Gmbh Vorrichtung und Verfahren zur Sterilisation flüssiger Medien mittels UV-Bestrahlung und Kurzzeiterhitzung
US20050100965A1 (en) * 2003-11-12 2005-05-12 Tariq Ghayur IL-18 binding proteins
ES2605406T3 (es) * 2004-01-21 2017-03-14 Toray Industries, Inc. Fraccionador
PL1720972T3 (pl) * 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
US7422875B2 (en) 2004-07-20 2008-09-09 Board Of Regents Of The University Of Nebraska Compositions and methods for increasing protein production
US8053238B2 (en) 2005-10-31 2011-11-08 Unhwa Corporation Isolated population of plant single cells and method of preparing the same
FI2343362T4 (fi) 2006-07-14 2024-07-04 Patheon Holdings I B V Parannettu menetelmä solujen viljelyä varten
US20080206819A1 (en) * 2006-08-21 2008-08-28 Mary Tsao Intensified Perfusion Production Method
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
US20100221823A1 (en) 2007-06-11 2010-09-02 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
EP2014760A1 (en) * 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
WO2008157247A1 (en) 2007-06-15 2008-12-24 Amgen Inc. Methods of treating cell culture media for use in a bioreactor
WO2009023562A2 (en) * 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
SI2574677T1 (sl) 2007-12-27 2017-11-30 Baxalta GmbH Procesi celične kulture
DE102008051574A1 (de) 2008-10-14 2010-04-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Herstellung von Interferon-beta und deren Varianten
PT2356247E (pt) 2008-11-12 2015-10-26 Baxalta Inc Método de produção de fator vii isento de soro e de insulina
NZ597727A (en) 2009-07-31 2013-08-30 Baxter Int Cell culture medium for adamts protein expression
US20110117087A1 (en) * 2009-10-26 2011-05-19 Reinhard Franze Method for the production of a glycosylated immunoglobulin
WO2011077035A1 (fr) * 2009-12-23 2011-06-30 Sanofi Pasteur Procede de culture de cellules adherentes
CN103298538B (zh) 2010-08-25 2017-05-10 瑞普利根公司 流体过滤系统
CN103347595B (zh) 2011-02-10 2016-01-20 克鲁塞尔荷兰公司 气动交变压力膜细胞分离系统
EP2678423A1 (en) 2011-02-23 2014-01-01 Amgen Inc. Cell culture media for uvc exposure and methods related thereto
US9133493B2 (en) 2011-04-21 2015-09-15 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
RU2600886C2 (ru) 2011-05-13 2016-10-27 Октафарма Аг Способ повышения продуктивности эукариотических клеток в продуцировании рекомбинантного fviii
SI2726600T1 (sl) 2011-07-01 2017-07-31 Amgen Inc. Sesalska celična kultura
BR112014008303A2 (pt) 2011-09-16 2017-04-11 Amgen Inc alimentação contínua controlada pré-programada sem feedback de culturas celulares
AU2012328753A1 (en) 2011-10-26 2014-04-24 Amgen Inc. Methods of reducing or eliminating protein modification and degradation arising from exposure to UV light
WO2013138159A1 (en) 2012-03-15 2013-09-19 Amgen Inc. Methods of determining exposure to uv light
US10481164B2 (en) 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
WO2014022102A1 (en) 2012-08-01 2014-02-06 Amgen Inc. Methods of using anti-apoptotic compounds to modulate one or more properties of a cell culture
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
ES2625510T3 (es) * 2012-11-26 2017-07-19 Gambro Lundia Ab Sistema de soporte hepático
ES2842350T3 (es) * 2013-03-19 2021-07-13 Cmc Biologics As Un procedimiento para la producción de un producto (por ejemplo, polipéptido) en un proceso de fermentación de cultivo celular continuo

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