ES2628847T3 - Producción de isoprenoides derivados de acetil-coenzima a - Google Patents

Producción de isoprenoides derivados de acetil-coenzima a Download PDF

Info

Publication number
ES2628847T3
ES2628847T3 ES12795948T ES12795948T ES2628847T3 ES 2628847 T3 ES2628847 T3 ES 2628847T3 ES 12795948 T ES12795948 T ES 12795948T ES 12795948 T ES12795948 T ES 12795948T ES 2628847 T3 ES2628847 T3 ES 2628847T3
Authority
ES
Spain
Prior art keywords
coenzyme
acetyl
production
heterologous nucleic
isoprenoids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES12795948T
Other languages
English (en)
Inventor
Timothy Stevens Gardner
Kristy Michelle Hawkins
Adam Leon Meadows
Annie Ening Tsong
Yoseph Tsegaye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Application granted granted Critical
Publication of ES2628847T3 publication Critical patent/ES2628847T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Una célula de levadura modificada genéticamente capaz de producir un isoprenoide, comprendiendo la célula: (a) uno o más ácidos nucleicos heterólogos que codifican una o más enzimas de una ruta de mevalonato (MEV) para fabricar isopentenil pirofosfato; y (b) un ácido nucleico heterólogo que codifica una acetilaldehído deshidrogenasa de acilación (ADA).
ES12795948T 2011-11-09 2012-11-09 Producción de isoprenoides derivados de acetil-coenzima a Active ES2628847T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161557893P 2011-11-09 2011-11-09
PCT/US2012/064532 WO2013071172A1 (en) 2011-11-09 2012-11-09 Production of acetyl-coenzyme a derived isoprenoids

Publications (1)

Publication Number Publication Date
ES2628847T3 true ES2628847T3 (es) 2017-08-04

Family

ID=47295179

Family Applications (1)

Application Number Title Priority Date Filing Date
ES12795948T Active ES2628847T3 (es) 2011-11-09 2012-11-09 Producción de isoprenoides derivados de acetil-coenzima a

Country Status (16)

Country Link
US (4) US8415136B1 (es)
EP (1) EP2776571B1 (es)
JP (2) JP6073350B2 (es)
KR (1) KR102021914B1 (es)
CN (1) CN104039974B (es)
AU (1) AU2012335091B2 (es)
BR (1) BR112014010750B1 (es)
CA (1) CA2853679C (es)
DK (1) DK2776571T3 (es)
ES (1) ES2628847T3 (es)
IN (1) IN2014CN03791A (es)
MX (1) MX346459B (es)
PT (1) PT2776571T (es)
SG (1) SG11201402083XA (es)
WO (1) WO2013071172A1 (es)
ZA (1) ZA201402989B (es)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2510666T3 (es) * 2004-03-31 2014-10-21 Cargill, Incorporated Proceso para fermentar azúcares que contienen sacáridos oligómeros
EP2739742A1 (en) * 2011-08-05 2014-06-11 Danisco US Inc. PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS
BR112014008278A2 (pt) * 2011-10-07 2017-04-18 Danisco Us Inc utilização de fosfocetolase na produção de mevalonato, precursores isoprenoides e isopreno
KR102021914B1 (ko) * 2011-11-09 2019-09-17 아미리스 인코퍼레이티드 아세틸-코엔자임 a로부터 유래되는 이소프레노이드의 제조 방법
BR112015012946B1 (pt) * 2012-12-10 2021-09-28 Cargill, Incorporated Processo de fermentação em lote para fermentar um hidrolisato de amido
CN105189772B (zh) 2013-03-15 2019-09-13 阿迈瑞斯公司 磷酸转酮酶和磷酸转乙酰酶生产乙酰-辅酶a衍生化合物的用途
EP3425049A1 (en) * 2013-04-10 2019-01-09 Danisco US Inc. Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids
US9617569B2 (en) 2013-11-15 2017-04-11 Samsung Electronics Co., Ltd. Genetically engineered yeast cell producing lactate including acetaldehyde dehydrogenase, method of producing yeast cell, and method of producing lactate using the same
WO2015077752A1 (en) 2013-11-25 2015-05-28 Genomatica, Inc. Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol
PL3077501T3 (pl) 2013-12-03 2022-01-31 Genomatica, Inc. Mikroorganizmy i sposoby poprawy wydajności produktu na metanolu z użyciem syntezy acetylo-coa
AU2014368982B2 (en) 2013-12-19 2021-03-25 Amyris, Inc. Methods for genomic integration
WO2015148272A1 (en) * 2014-03-28 2015-10-01 Danisco Us Inc. Altered host cell pathway for improved ethanol production
JP6034332B2 (ja) 2014-05-30 2016-11-30 トヨタ自動車株式会社 組換え微生物及び当該組換え微生物を用いた物質製造方法
MY185817A (en) 2014-08-21 2021-06-10 Manus Biosynthesis Inc Methods for production of oxygenated terpenes
WO2016039685A1 (en) * 2014-09-08 2016-03-17 Temasek Life Sciences Laboratory Limited Methods for efficient production of polyunsaturated fatty acids (pufa) in rhodosporidium and rhodotorula species
JP6470532B2 (ja) * 2014-09-17 2019-02-13 積水化学工業株式会社 組換え細胞、並びに、有機化合物の生産方法
US20170298363A1 (en) 2014-09-18 2017-10-19 Genomatica, Inc Non-natural microbial organisms with improved energetic efficiency
US20180127804A1 (en) 2014-12-05 2018-05-10 Amyris, Inc. High-throughput sequencing of polynucleotides
DE102015103608A1 (de) * 2015-03-11 2016-09-15 Basf Se Verfahren zur mikrobiellen de novo Synthese von Terpenen
KR101782891B1 (ko) * 2015-07-08 2017-09-29 한국과학기술연구원 미어신 생산용 벡터 및 균주, 및 이를 이용한 미어신의 생산방법
WO2017075538A1 (en) 2015-10-29 2017-05-04 Amyris, Inc. Compositions and methods for production of myrcene
CA3003163A1 (en) 2015-10-29 2017-05-04 Senomyx, Inc. High intensity sweeteners
US20180334657A1 (en) * 2015-11-30 2018-11-22 Sekisui Chemical Co., Ltd. Recombinant cell, method for producing recombinant cell, and method for producing organic compound
EP3478844A4 (en) * 2016-07-01 2020-04-08 International Flavors & Fragrances Inc. COMPOSITIONS AND METHODS FOR PRODUCING CITRUS TERPENOIDS
EP3555295B1 (en) * 2016-12-16 2021-01-20 Danisco US Inc. Bifunctional phosphoketolase-phosphotransacetylase fusion polypeptides
WO2018140652A1 (en) 2017-01-25 2018-08-02 Amyris, Inc. Co-production of a sesquiterpene and a carotenoid
CN106893726B (zh) * 2017-02-20 2020-06-09 天津大学 一种启动子以及重组酵母菌株
EP3587572A4 (en) * 2017-02-27 2020-12-30 Sekisui Chemical Co., Ltd. RECOMBINATED CELL, PROCESS FOR THE PRODUCTION OF A RECOMBINATED CELL AND PROCESS FOR THE PRODUCTION OF ISOPRENE OR TERPENE
BR112019023104A2 (pt) 2017-05-03 2020-11-03 Firmenich Incorporated métodos para fazer adoçante de alta intensidade
WO2019030073A1 (en) 2017-08-07 2019-02-14 Total Raffinage Chimie PROCESS FOR RECOVERING ISOPRENOIDS PRODUCED BY MICROORGANISMS
WO2019030007A1 (en) 2017-08-07 2019-02-14 Total Raffinage Chimie PROCESS FOR RECOVERING FERMENTATION PRODUCTS
JP2019041712A (ja) * 2017-09-05 2019-03-22 株式会社デンソー 組み換え体及びスクアレンの製造方法
CN107841523A (zh) * 2017-10-19 2018-03-27 浙江大学 利用群体感应分子诱导从桦褐孔菌中提取三萜类物质方法
CN107937431A (zh) * 2017-10-28 2018-04-20 国际竹藤中心 一种提高酿酒酵母合成芳樟醇能力的方法
KR20200095534A (ko) 2017-12-07 2020-08-10 지머젠 인코포레이티드 발효에 의한 (6e)-8-히드록시제라니올의 제조를 위해 조작된 생합성 경로
WO2019126778A1 (en) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone
SG11202103802XA (en) 2018-10-15 2021-05-28 Amyris Inc Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
MX2021005219A (es) 2018-11-07 2021-06-18 Firmenich Incorporated Metodos para hacer edulcorantes de alta intensidad.
CN111378587B (zh) * 2018-12-29 2022-08-05 中国科学院青岛生物能源与过程研究所 一种合成β-法尼烯的基因工程菌及其应用
CN111378588A (zh) * 2018-12-29 2020-07-07 中国科学院青岛生物能源与过程研究所 一种转化纤维素水解液合成法尼烯的基因工程菌及其应用
MX2021008747A (es) 2019-01-24 2021-09-08 Amyris Inc Transportadores abc para la produccion de alta eficiencia de rebaudiosidos.
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用
CN109929937B (zh) * 2019-04-01 2023-04-21 广东省科学院动物研究所 龟嗜皮菌特异性鉴定引物、试剂盒及其鉴定方法
US12065685B1 (en) 2019-05-13 2024-08-20 Amyris, Inc. UDP-glycosyltransferase variants and uses thereof
GB201907088D0 (en) 2019-05-20 2019-07-03 C3 Bio Tech Limited Linalool synthases
BR112021024508A2 (pt) * 2019-06-06 2022-03-22 Amyris Inc Métodos para desacoplar rendimento e produtividade de um composto não catabólico produzido por uma célula hospedeira
CA3171369A1 (en) * 2020-02-28 2021-09-02 Wisconsin Alumni Research Foundation Nonviral generation of genome edited chimeric antigen receptor t cells
US20230174922A1 (en) * 2020-07-08 2023-06-08 The Regents Of The University Of California Modified yeast host cells useful for producing isoprenol
CN113234610B (zh) * 2021-03-05 2023-06-13 江南大学 一种合成角鲨烯的酿酒酵母菌株及其应用
CN113430215B (zh) * 2021-06-03 2023-04-18 昆明理工大学 乙酰CoA合成酶基因RKACS1及其应用
CN115704038B (zh) * 2021-08-10 2025-08-15 西北农林科技大学 一种基因、重组载体、工程菌及其应用
CN116376995B (zh) * 2022-04-02 2024-01-26 元素驱动(杭州)生物科技有限公司 一种利用苏氨酸制备甘氨酸、乙酰辅酶a及乙酰辅酶a衍生物的方法
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids
EP4646426A1 (en) 2023-01-03 2025-11-12 Amyris, Inc. Host cells capable of producing sequiterpenoids and methods of use thereof
EP4658757A1 (en) 2023-02-03 2025-12-10 Amyris, Inc. Host cells capable of producing retinol or retinol precursors and methods of use thereof
KR20260010506A (ko) 2023-05-15 2026-01-20 디에스엠 아이피 어셋츠 비.브이. 발효 공정 중 에멀젼 형성 감소 방법

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR745875A (fr) 1932-11-16 1933-05-17 Système d'adaptation aux brouettes, de roues à bandage pneumatique
US5272065A (en) 1983-10-20 1993-12-21 Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
US7253001B2 (en) 2002-03-19 2007-08-07 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of Saccharomyces cerevisiae
ES2379368T3 (es) * 2004-05-21 2012-04-25 The Regents Of The University Of California Método para aumentar la producción de compuestos isoprenoides
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
EP1778843B1 (en) * 2004-08-19 2012-09-19 DSM IP Assets B.V. Production of isoprenoids
WO2007024718A2 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Genetically modified host cells and use of same for producing isoprenoid compounds
WO2007086608A1 (en) 2006-01-27 2007-08-02 Ajinomoto Co., Inc. Method for producing l-amino acid
CA2651747C (en) 2006-05-26 2017-10-24 Amyris Biotechnologies, Inc. Production of isoprenoids
WO2009005704A1 (en) * 2007-07-03 2009-01-08 The Regents Of The University Of California Methods of increasing isoprenoid or isoprenoid precursor production
US20100248233A1 (en) 2007-07-23 2010-09-30 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
AU2009316660B2 (en) 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
EP2438157A1 (en) * 2009-06-01 2012-04-11 Amyris, Inc. Genetically modified microbes producing isoprenoids
EP2277989A1 (en) * 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
KR20190006103A (ko) * 2010-05-05 2019-01-16 게노마티카 인코포레이티드 부타디엔을 생합성하기 위한 미생물 및 방법
JP5056897B2 (ja) 2010-05-14 2012-10-24 トヨタ自動車株式会社 ２−ブタノールの製造方法及び２−ブタノール生産能を有する組換え微生物
DE102010029973A1 (de) * 2010-06-11 2011-12-15 Evonik Degussa Gmbh Mikrobiologische Herstellung von C4-Körpern aus Saccharose und Kohlendioxid
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
BR112014002661A2 (pt) 2011-08-04 2019-09-24 Danisco Us Inc produção de isopreno, precursores de isoprenoide, e isoprenoides com o uso de acetoacetil-coa sintase
KR102021914B1 (ko) * 2011-11-09 2019-09-17 아미리스 인코퍼레이티드 아세틸-코엔자임 a로부터 유래되는 이소프레노이드의 제조 방법

Also Published As

Publication number Publication date
MX2014005543A (es) 2014-06-04
EP2776571A1 (en) 2014-09-17
US20140154765A1 (en) 2014-06-05
JP6073350B2 (ja) 2017-02-01
US20150093797A1 (en) 2015-04-02
JP2014532444A (ja) 2014-12-08
CA2853679A1 (en) 2013-05-16
CN104039974A (zh) 2014-09-10
PT2776571T (pt) 2017-07-07
BR112014010750A2 (pt) 2017-06-13
MX346459B (es) 2017-03-22
US20130236942A1 (en) 2013-09-12
CN104039974B (zh) 2016-10-12
KR20140093981A (ko) 2014-07-29
BR112014010750B1 (pt) 2021-09-21
US8603800B2 (en) 2013-12-10
DK2776571T3 (en) 2017-06-26
CA2853679C (en) 2021-05-04
IN2014CN03791A (es) 2015-10-16
AU2012335091B2 (en) 2016-06-16
EP2776571B1 (en) 2017-04-12
US8415136B1 (en) 2013-04-09
JP2017055777A (ja) 2017-03-23
KR102021914B1 (ko) 2019-09-17
SG11201402083XA (en) 2014-06-27
AU2012335091A1 (en) 2014-06-19
US8859261B2 (en) 2014-10-14
ZA201402989B (en) 2015-11-25
WO2013071172A1 (en) 2013-05-16
US9914941B2 (en) 2018-03-13
JP6461208B2 (ja) 2019-01-30

Similar Documents

Publication Publication Date Title
MX347976B (es) Celulas huesped y metodos para producir isobutanol.
MX353884B (es) Produccion de alquenos a traves de la conversion enzimatica combinada de acidos 3-hidroxialcanoicos.
BR112013010551A8 (pt) variantes de isopreno sintase para produção aprimorada de isopreno
MX2018004614A (es) Metodos para preparar celulas t para terapia de celulas t.
BR112012011073B8 (pt) Método para aumentar a produção de terpenoide em uma célula que produz um ou mais terpenoides
MX2016014235A (es) Biosintesis microbiana de ergotioneina.
MX2012013037A (es) Composiciones de endorribonucleasa y metodos de uso de las mismas.
EA036334B9 (ru) Генетически модифицированная бактерия clostridium для получения салицилата из хоризмата
UA112763C2 (uk) Алелі 1-дезокси-d-ксилулозо-5-фосфатсинтази, відповідальні за посилений біосинтез терпенів
BR112017018885A2 (pt) método de cultura celular para células-tronco mesenquimais
PH12013500349A1 (en) Differentiation of pluripotent stem cells
BR112015011088A2 (pt) Método para sintetizar isobuteno e hospedeiro recombinante
BR112018002214A2 (pt) ?métodos para cultivar células e para produzir uma proteína, meios de cultura de células cho e de batelada alimentada e/ou alimentação, e, alimentação de cultura de células cho?
MX346459B (es) Produccion de isoprenoides derivados de acetil - coenzima a.
AR092478A1 (es) Loci de performance de fad3 y proteinas de union especificas de un sitio objetivo correspondiente capaces de inducir rupturas direccionadas
MX392155B (es) Composiciones y metodos para la biosintesis de vainillina o de beta-d-glucosido de vainillina.
WO2012135591A3 (en) Microbial isoprenoid production using a heterologous dxp pathway
ES2721920T3 (es) Uso de fosfocetolasa y fosfotransacetilasa para la producción de compuestos derivados de acetil-coenzima A
WO2016168890A8 (en) Generation of muscle-lineage cells from stem cells
CL2012002634A1 (es) Microorganismo transgenico que comprende por lo menos un gen que codifica la glucosa-6-fosfato deshidrogenasa, por lo menos un gen que codifica 6-fosfogluconolactonasa y por lo menos un gen heterologo que codifica un producto no nativo de interes; levadura oleaginosa transgenica; y metodo para la produccion de un producto no nativo de interes.
BR112016029399A2 (pt) métodos, reagentes e células para biossintetizar composto
WO2012177129A3 (en) Valencene synthase
BR112015030208A2 (pt) Métodos para produzir um produto de fermentação e um micro-organismo, e, micro-organismo recombinante
CL2012002554A1 (es) Metodo para la produccion de carotenoides incluyendo astaxantina, que comprende cultivar una bacteria que produce en forma concurrente astaxantina y cantaxantina en un medio que contiene biotina; y composicion de carotenoides.
PH12014501161A1 (en) Biotechnological production of 3-hydroxyisobutyric acid