ES2638853T3 - Amplificación isotérmica de ácido nucleico - Google Patents
Amplificación isotérmica de ácido nucleico Download PDFInfo
- Publication number
- ES2638853T3 ES2638853T3 ES09762016.5T ES09762016T ES2638853T3 ES 2638853 T3 ES2638853 T3 ES 2638853T3 ES 09762016 T ES09762016 T ES 09762016T ES 2638853 T3 ES2638853 T3 ES 2638853T3
- Authority
- ES
- Spain
- Prior art keywords
- target molecule
- nucleic acid
- primer
- molecule
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 230000003321 amplification Effects 0.000 title description 2
- 238000003199 nucleic acid amplification method Methods 0.000 title description 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract description 6
- 230000000295 complement effect Effects 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 102000018120 Recombinases Human genes 0.000 abstract 1
- 108010091086 Recombinases Proteins 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 description 2
- 108020000005 Sucrose phosphorylase Proteins 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 102000002281 Adenylate kinase Human genes 0.000 description 1
- 108020000543 Adenylate kinase Proteins 0.000 description 1
- 102000004420 Creatine Kinase Human genes 0.000 description 1
- 108010042126 Creatine kinase Proteins 0.000 description 1
- 101800001466 Envelope glycoprotein E1 Proteins 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 101710085030 Gene 32 protein Proteins 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101800001690 Transmembrane protein gp41 Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229950007002 phosphocreatine Drugs 0.000 description 1
- 229920002523 polyethylene Glycol 1000 Polymers 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Un procedimiento isotérmico para amplificar una molécula objetivo de ácido nucleico bicatenario que comprende las siguientes etapas: (a) proporcionar: (i) cebadores en dirección 5' y en dirección 3', comprendiendo cada uno una molécula de ADN monocatenario de menos de 30 nucleótidos, al menos una porción de la cual es complementaria a una secuencia de la molécula objetivo; (ii) un oligonucleótido que comprende una molécula de ADN monocatenario de al menos 30 nucleótidos, al menos una porción de la cual es complementaria a una secuencia de la molécula objetivo que interviene en los cebadores directo e inverso, en donde el oligonucleótido tiene un extremo terminal 3'; (b) poner en contacto el oligonucleótido (ii) con la recombinasa para permitir que invada la región complementaria de la molécula objetivo, volviendo de este modo la región complementaria de la molécula objetivo y regiones adyacentes monocatenarias; (c) aplicar el cebador en dirección 5' a la región monocatenaria de la molécula objetivo y extender el extremo terminal 3' del cebador en dirección 5' con polimerasa y dNTP para producir una molécula objetivo de ácido nucleico bicatenario; (d) aplicar el cebador en dirección 3' a la molécula objetivo monocatenaria y extender el extremo terminal 3' del cebador en dirección 3' con polimerasa y dNTP para producir otra molécula objetivo de ácido nucleico bicatenario; (e) continuar la reacción mediante la repetición de (b) hasta (d).
Description
Sacarosa fosforilasa (Sigma S0937) Se disolvió hasta 0,4 u/µL en glicerol al 40%/H2O UvsX, UvsY; 100 µM en acetato de K 300 mM; glicerol al 50%. Gp32 (NEB, 10 mg/mL) Klenow, exo -(Jena Biosciences, 50 u/µL) utilizado a una concentración final de 0,05 u/µL
- Componente
- Concentración final de reacción
- Tris pH 8
- 10 mM
- Acetato de Mg
- 10 mM
- BSA**
- 0,1 mg/mL
- DTT**
- 5 mM
- DMSO**
- 5%
- PEG 1000**
- 5%
- Sacarosa**
- 150 mM
- ATP
- 2mM
- dNTP
- 200 µM
- Sybr Green*
- 1:100.000
- Oligonucleótidos
- Como se muestra en los ejemplos
- gp32
- 0,5 µM
- Fosfocreatina (diTRIS) pH hasta 7,8 con KOH
- 75 mM
- Creatina Quinasa
- 1µM
- Mioquinasa**
- 1µM
- Pirofosfatasa**
- 1µM
- UvsY
- 1,5 µM
- UvsX
- 1,5 µM
- Sacarosa fosforilasa**
- 1µM
- Klenow
- 0,1µM
- ADN plantilla
- Como se muestra en los ejemplos
- ** = componentes que se encuentra que optimizan, pero que no son esenciales para la amplificación.
Se añadió plantilla de ensayo a una mezcla de los componentes de reacción excepto por UvsX y Klenow. Los componentes de reacción se incubaron con una muestra de ensayo durante 5 minutos a la temperatura de trabajo (40° C) y se añadieron UvsX y Klenow. Los volúmenes totales de muestra fueron de 20 µL colocados en placas de
10 microtitulación de 384 pocillos de bajo volumen. La fluorescencia se evaluó en un BMG-fluostar-II. La fluorescencia se controló a intervalos de un minuto por excitación a 480 nm y leyendo la emisión a 520 nm para la fluorescencia Sybr Green (a menos que se indique lo contrario).
Ejemplo 1: Sistema de dos cebadores, sin oligonucleótido intermedio (sistema de la técnica anterior)
El protocolo utilizado es el mismo que aquel descrito anteriormente a menos que se indique lo contrario. Los 15 constituyentes de oligonucleótidos comprendían dos cebadores a una concentración final de 150 nM junto con la
16
Claims (1)
-
imagen1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0810650 | 2008-06-11 | ||
| GB0810650A GB0810650D0 (en) | 2008-06-11 | 2008-06-11 | Strand invasion based isothermal nucleic acid amplification |
| GB0822533A GB0822533D0 (en) | 2008-12-11 | 2008-12-11 | Strand invasion based isothermal nucleic acid amplifications (2) |
| GB0822533 | 2008-12-11 | ||
| PCT/GB2009/050662 WO2009150467A1 (en) | 2008-06-11 | 2009-06-11 | Isothermal nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2638853T3 true ES2638853T3 (es) | 2017-10-24 |
Family
ID=40872336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09762016.5T Active ES2638853T3 (es) | 2008-06-11 | 2009-06-11 | Amplificación isotérmica de ácido nucleico |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US9062344B2 (es) |
| EP (3) | EP2304054B1 (es) |
| JP (2) | JP5798917B2 (es) |
| CN (2) | CN102119225B (es) |
| CA (1) | CA2727212C (es) |
| CY (2) | CY1119346T1 (es) |
| DK (2) | DK2304054T3 (es) |
| ES (1) | ES2638853T3 (es) |
| HR (2) | HRP20171286T1 (es) |
| HU (2) | HUE036005T2 (es) |
| LT (2) | LT2660336T (es) |
| PL (2) | PL2660336T3 (es) |
| PT (2) | PT2304054T (es) |
| SI (2) | SI2660336T1 (es) |
| WO (1) | WO2009150467A1 (es) |
| ZA (1) | ZA201008749B (es) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
| SI2660336T1 (sl) | 2008-06-11 | 2017-11-30 | Orion Pharma (Uk) Limited | Izotermna ojačitev nukleinske kisline |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| EP3564392B1 (en) | 2010-12-17 | 2021-11-24 | Life Technologies Corporation | Methods for nucleic acid amplification |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| US10093975B2 (en) | 2011-12-01 | 2018-10-09 | Genapsys, Inc. | Systems and methods for high efficiency electronic sequencing and detection |
| CN114854832A (zh) * | 2012-04-19 | 2022-08-05 | 生命技术公司 | 核酸扩增 |
| CN102816756B (zh) * | 2012-09-07 | 2014-04-16 | 江苏奇天基因生物科技有限公司 | 等温核酸扩增反应试剂及等温核酸扩增方法 |
| JP2016521120A (ja) | 2013-03-15 | 2016-07-21 | セラノス， インコーポレイテッド | 核酸増幅 |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| EP2971141B1 (en) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systems for biological analysis |
| AU2014233145A1 (en) | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
| AU2014233152A1 (en) | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
| CN105358711A (zh) * | 2013-04-25 | 2016-02-24 | 欧雷恩诊断公司 | 基于链侵入的dna扩增方法 |
| WO2015035260A1 (en) | 2013-09-06 | 2015-03-12 | Theranos, Inc. | Systems and methods for detecting infectious diseases |
| CN106414764B (zh) * | 2013-11-22 | 2020-10-30 | 欧雷恩诊断公司 | 通过基于链侵入的扩增的核酸检测 |
| WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
| US9822401B2 (en) | 2014-04-18 | 2017-11-21 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| GB201410022D0 (en) * | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
| US11268117B2 (en) * | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| WO2018017884A1 (en) | 2016-07-20 | 2018-01-25 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| ES3008759T3 (en) * | 2016-08-02 | 2025-03-25 | Hoffmann La Roche | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
| WO2018038232A1 (ja) * | 2016-08-24 | 2018-03-01 | 国立大学法人東北大学 | 標的核酸の増幅産物の生産方法及びその利用 |
| GB201618035D0 (en) | 2016-10-25 | 2016-12-07 | Orion Diagnostica Oy | Method |
| CN107058287B (zh) * | 2017-01-16 | 2020-06-16 | 浙江大学 | 一种恒温扩增体系中生成单链产物的方法 |
| WO2019060628A1 (en) | 2017-09-21 | 2019-03-28 | Genapsys, Inc. | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING |
| CN107893103A (zh) * | 2017-11-29 | 2018-04-10 | 默禾医疗科技（上海）有限公司 | 重组酶聚合酶扩增中重组酶和蛋白浓度比及活性定量法 |
| EP3530755B1 (de) * | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
| CA3122844A1 (en) * | 2019-01-15 | 2020-07-23 | Tangen Bioscience Inc. | A method for suppressing non-specific amplification products in nucleic acid amplification technologies |
| CN111926119B (zh) * | 2020-09-03 | 2023-04-21 | 上海市计量测试技术研究院 | 一种用于检测新型冠状病毒的核酸检测试剂盒及其使用方法 |
| GB202018125D0 (en) | 2020-11-18 | 2020-12-30 | Aidian Oy | Method |
| NL2033124B1 (en) | 2022-09-23 | 2024-03-29 | Rapidemic B V | Methods and device for multiple-label nucleic acid amplification and detection |
| WO2026015456A1 (en) * | 2024-07-08 | 2026-01-15 | Natera, Inc. | Methods for maintaining methylation in amplification of methylated nucleic acid molecules |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| WO1999055914A1 (en) * | 1998-04-29 | 1999-11-04 | Trustees Of Boston University | Methods and compositions pertaining to pd-loops |
| US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
| JP4707237B2 (ja) * | 1999-03-17 | 2011-06-22 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | 外因性アミノ酸および新規ａｔｐ再生システムを用いたインビトロ高分子生合成の方法 |
| JP2003507071A (ja) * | 1999-08-20 | 2003-02-25 | ロシュ ダイアグノスティックス ゲーエムベーハー | デオキシリボヌクレオシドの酵素的合成 |
| MXPA02002656A (es) | 1999-09-13 | 2003-10-14 | Nugen Technologies Inc | Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos. |
| US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| ES2425354T3 (es) * | 2004-06-01 | 2013-10-14 | Alere San Diego, Inc. | Amplificación por recombinasa-polimerasa |
| WO2006051988A1 (ja) | 2004-11-15 | 2006-05-18 | Riken | 核酸の増幅方法 |
| WO2006087574A2 (en) | 2005-02-19 | 2006-08-24 | Geneform Technologies Limited | Isothermal nucleic acid amplification |
| JP2006271372A (ja) | 2005-03-01 | 2006-10-12 | Yamasa Shoyu Co Ltd | 糖鎖の製造法 |
| JP5027126B2 (ja) * | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ， インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| SI2660336T1 (sl) | 2008-06-11 | 2017-11-30 | Orion Pharma (Uk) Limited | Izotermna ojačitev nukleinske kisline |
-
2009
- 2009-06-11 SI SI200931719T patent/SI2660336T1/sl unknown
- 2009-06-11 DK DK09762016.5T patent/DK2304054T3/en active
- 2009-06-11 JP JP2011513055A patent/JP5798917B2/ja active Active
- 2009-06-11 CN CN200980131223.9A patent/CN102119225B/zh active Active
- 2009-06-11 HU HUE09762016A patent/HUE036005T2/hu unknown
- 2009-06-11 PT PT97620165T patent/PT2304054T/pt unknown
- 2009-06-11 EP EP09762016.5A patent/EP2304054B1/en active Active
- 2009-06-11 LT LTEP13178545.3T patent/LT2660336T/lt unknown
- 2009-06-11 LT LTEP09762016.5T patent/LT2304054T/lt unknown
- 2009-06-11 ES ES09762016.5T patent/ES2638853T3/es active Active
- 2009-06-11 DK DK13178545.3T patent/DK2660336T3/en active
- 2009-06-11 EP EP17179338.3A patent/EP3249056B1/en active Active
- 2009-06-11 US US12/997,382 patent/US9062344B2/en active Active
- 2009-06-11 HU HUE13178545A patent/HUE034561T2/en unknown
- 2009-06-11 EP EP13178545.3A patent/EP2660336B1/en active Active
- 2009-06-11 PL PL13178545T patent/PL2660336T3/pl unknown
- 2009-06-11 CN CN201510103597.7A patent/CN104862385B/zh active Active
- 2009-06-11 CA CA2727212A patent/CA2727212C/en active Active
- 2009-06-11 PL PL09762016T patent/PL2304054T3/pl unknown
- 2009-06-11 SI SI200931718T patent/SI2304054T1/sl unknown
- 2009-06-11 HR HRP20171286TT patent/HRP20171286T1/hr unknown
- 2009-06-11 PT PT131785453T patent/PT2660336T/pt unknown
- 2009-06-11 WO PCT/GB2009/050662 patent/WO2009150467A1/en not_active Ceased
-
2010
- 2010-12-06 ZA ZA2010/08749A patent/ZA201008749B/en unknown
-
2015
- 2015-05-12 US US14/709,793 patent/US9657340B2/en active Active
- 2015-08-21 JP JP2015164059A patent/JP6301881B2/ja active Active
-
2017
- 2017-04-12 US US15/485,689 patent/US10472659B2/en active Active
- 2017-08-31 CY CY20171100921T patent/CY1119346T1/el unknown
- 2017-09-08 HR HRP20171356TT patent/HRP20171356T1/hr unknown
- 2017-09-11 CY CY20171100958T patent/CY1119331T1/el unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2638853T3 (es) | Amplificación isotérmica de ácido nucleico | |
| ES2639938T5 (es) | Métodos y composiciones para la secuenciación de ácidos nucleicos | |
| Navarro et al. | Real-time PCR detection chemistry | |
| Hu et al. | Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level | |
| TWI704229B (zh) | 核酸定序方法 | |
| CN106434871B (zh) | 用于检测目标核酸的方法与组合物 | |
| CN102827836B (zh) | 一种寡核苷酸探针以及用其对靶分子进行检测的方法 | |
| US20250236910A1 (en) | Methods for single molecule sequencing | |
| AU2021290387B2 (en) | Labeled nucleotide compositions and methods for nucleic acid sequencing | |
| US9809846B2 (en) | Compositions, kits, uses and methods for amplified detection of an analyte | |
| CN109562376A (zh) | 一种基于荧光能量转移的单分子/集群dna合成测序 | |
| EP2766498A2 (en) | Sequencing by structure assembly | |
| WO2010017932A4 (en) | Proofreading primer extension | |
| IL316759A (en) | Preparations and methods for identifying biological contaminants | |
| CN105087825A (zh) | 常温等温快速检测埃博拉病毒的方法、试剂及引物和探针 | |
| RU2016116333A (ru) | Определение нуклеиновых кислот путем амплификации, основанной на встраивании в цепь | |
| CN113088557B (zh) | 一种同时检测多种dna糖基化酶的荧光化学传感器及其检测方法和应用 | |
| JP2018523991A (ja) | 構造スイッチングのタンデム反応、核酸分解消化および核酸アセンブリーの増幅を含むバイオセンサー | |
| CN110997934A (zh) | 具有电子检测系统的寡核苷酸探针阵列 | |
| Zhang et al. | A rapid label-and enzyme-free G-quadruplex-based fluorescence strategy for highly-sensitive detection of HIV DNA | |
| Chen et al. | Enzyme-free and sensitive method for single-stranded nucleic acid detection based on CHA and HCR | |
| US11761040B2 (en) | DNA protection agent in DNA imaging buffer | |
| Liu et al. | Amplified detection of T4 polynucleotide kinase activity based on a λ-exonuclease cleavage-induced DNAzyme releasing strategy | |
| CN109536577B (zh) | 一种末端脱氧核酸酶活力的测定方法与应用 | |
| Cheng et al. | Ligase chain reaction coupled with rolling circle amplification for high sensitivity detection of single nucleotide polymorphisms |