CY1119331T1 - Ισοθερμικη ενισχυση νουκλεικων οξεων - Google Patents
Ισοθερμικη ενισχυση νουκλεικων οξεωνInfo
- Publication number
- CY1119331T1 CY1119331T1 CY20171100958T CY171100958T CY1119331T1 CY 1119331 T1 CY1119331 T1 CY 1119331T1 CY 20171100958 T CY20171100958 T CY 20171100958T CY 171100958 T CY171100958 T CY 171100958T CY 1119331 T1 CY1119331 T1 CY 1119331T1
- Authority
- CY
- Cyprus
- Prior art keywords
- oligonucleotide
- target
- isothermal
- aucation
- nucleic acid
- Prior art date
Links
- 239000002253 acid Substances 0.000 title 1
- 150000007513 acids Chemical class 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 238000011144 upstream manufacturing Methods 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Μία ισοθερμική διαδικασία για την ενίσχυση ενός μορίου νουκλεϊκού οξέος-στόχου που βασίζεται σε έναν ανοδικό εκκινητή, έναν καθοδικό εκκινητή και ένα ολιγονουκλεοτίδιο, όπου τουλάχιστον ένα τμήμα του ολιγονουκλεοτιδίου είναι συμπληρωματικό σε ένα τμήμα της αλληλουχίας-στόχου που παρεμβαίνει στους ανοδικούς και καθοδικούς εκκινητές. Οι ανοδικοί και οι καθοδικοί εκκινητές δεν ενισχύουν το μόριο-στόχου ανεξάρτητα από το ολιγονουκλεοτίδιο και όπου το ολιγονουκλεοτίδιο διευκολύνει τον διαχωρισμό του μορίου-στόχου νουκλεϊκού οξέος με διπλή έλικα, επιτρέποντας έτσι στους εκκινητές να έχουν πρόσβαση να δεσμεύονται στο συμπληρωματικό μονόκλωνο DNA επί του μορίου-στόχου.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0810650A GB0810650D0 (en) | 2008-06-11 | 2008-06-11 | Strand invasion based isothermal nucleic acid amplification |
| GB0822533A GB0822533D0 (en) | 2008-12-11 | 2008-12-11 | Strand invasion based isothermal nucleic acid amplifications (2) |
| EP09762016.5A EP2304054B1 (en) | 2008-06-11 | 2009-06-11 | Isothermal nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1119331T1 true CY1119331T1 (el) | 2018-02-14 |
Family
ID=40872336
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20171100921T CY1119346T1 (el) | 2008-06-11 | 2017-08-31 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
| CY20171100958T CY1119331T1 (el) | 2008-06-11 | 2017-09-11 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20171100921T CY1119346T1 (el) | 2008-06-11 | 2017-08-31 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US9062344B2 (el) |
| EP (3) | EP2660336B1 (el) |
| JP (2) | JP5798917B2 (el) |
| CN (2) | CN102119225B (el) |
| CA (1) | CA2727212C (el) |
| CY (2) | CY1119346T1 (el) |
| DK (2) | DK2304054T3 (el) |
| ES (1) | ES2638853T3 (el) |
| HR (2) | HRP20171286T1 (el) |
| HU (2) | HUE034561T2 (el) |
| LT (2) | LT2304054T (el) |
| PL (2) | PL2304054T3 (el) |
| PT (2) | PT2304054T (el) |
| SI (2) | SI2304054T1 (el) |
| WO (1) | WO2009150467A1 (el) |
| ZA (1) | ZA201008749B (el) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
| EP2660336B1 (en) | 2008-06-11 | 2017-08-16 | Orion Pharma (UK) Limited | Isothermal nucleic acid amplification |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| CN106591103B (zh) | 2011-12-01 | 2021-06-04 | 吉纳普赛斯股份有限公司 | 用于高效电子测序与检测的系统和方法 |
| SG10201802883UA (en) * | 2012-04-19 | 2018-05-30 | Life Technologies Corp | Nucleic acid amplification |
| CN102816756B (zh) * | 2012-09-07 | 2014-04-16 | 江苏奇天基因生物科技有限公司 | 等温核酸扩增反应试剂及等温核酸扩增方法 |
| US20140302504A1 (en) | 2013-03-15 | 2014-10-09 | Theranos, Inc. | Nucleic Acid Amplification |
| SG11201507273SA (en) | 2013-03-15 | 2015-10-29 | Theranos Inc | Nucleic acid amplification |
| CN116334193A (zh) | 2013-03-15 | 2023-06-27 | 赛拉诺斯知识产权有限责任公司 | 核酸扩增 |
| EP2971141B1 (en) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systems for biological analysis |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| EP2989212B1 (en) * | 2013-04-25 | 2018-07-18 | Orion Diagnostica Oy | Strand-invasion based dna amplification method |
| SG11201601611WA (en) | 2013-09-06 | 2016-04-28 | Theranos Inc | Systems and methods for detecting infectious diseases |
| US11008625B2 (en) * | 2013-11-22 | 2021-05-18 | Aidian Oy | Detection of nucleic acids by strand invasion based amplification |
| WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
| EP3556864B1 (en) * | 2014-04-18 | 2020-12-09 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| GB201410022D0 (en) * | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
| US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| US10443095B2 (en) * | 2016-08-02 | 2019-10-15 | Roche Molecular Systems, Inc. | Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids |
| WO2018038232A1 (ja) * | 2016-08-24 | 2018-03-01 | 国立大学法人東北大学 | 標的核酸の増幅産物の生産方法及びその利用 |
| GB201618035D0 (en) | 2016-10-25 | 2016-12-07 | Orion Diagnostica Oy | Method |
| CN107058287B (zh) * | 2017-01-16 | 2020-06-16 | 浙江大学 | 一种恒温扩增体系中生成单链产物的方法 |
| CA3076378A1 (en) | 2017-09-21 | 2019-03-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| CN107893103A (zh) * | 2017-11-29 | 2018-04-10 | 默禾医疗科技（上海）有限公司 | 重组酶聚合酶扩增中重组酶和蛋白浓度比及活性定量法 |
| EP3530755B1 (de) * | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
| US20200224260A1 (en) * | 2019-01-15 | 2020-07-16 | Tangen Bioscience Inc. | Method for suppressing non-specific amplification products in nucleic acid amplification technologies |
| CN111926119B (zh) * | 2020-09-03 | 2023-04-21 | 上海市计量测试技术研究院 | 一种用于检测新型冠状病毒的核酸检测试剂盒及其使用方法 |
| GB202018125D0 (en) | 2020-11-18 | 2020-12-30 | Aidian Oy | Method |
| NL2033124B1 (en) | 2022-09-23 | 2024-03-29 | Rapidemic B V | Methods and device for multiple-label nucleic acid amplification and detection |
| WO2026015456A1 (en) * | 2024-07-08 | 2026-01-15 | Natera, Inc. | Methods for maintaining methylation in amplification of methylated nucleic acid molecules |
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| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| AU3671099A (en) * | 1998-04-29 | 1999-11-16 | Trustees Of Boston University | Methods and compositions pertaining to pd-loops |
| US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
| WO2000055353A1 (en) | 1999-03-17 | 2000-09-21 | The Board Of Trustees Of The Leland Stanford Junior University | In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system |
| US7229797B1 (en) * | 1999-08-20 | 2007-06-12 | Institut Pasteur | Enzymatic synthesis of deoxyribonucleosides |
| DK1218542T3 (da) * | 1999-09-13 | 2004-08-02 | Nugen Technologies Inc | Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser |
| US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| EP3985125A1 (en) * | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| WO2006051988A1 (ja) * | 2004-11-15 | 2006-05-18 | Riken | 核酸の増幅方法 |
| WO2006087574A2 (en) | 2005-02-19 | 2006-08-24 | Geneform Technologies Limited | Isothermal nucleic acid amplification |
| JP2006271372A (ja) | 2005-03-01 | 2006-10-12 | Yamasa Shoyu Co Ltd | 糖鎖の製造法 |
| WO2007096702A2 (en) * | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| US8071308B2 (en) * | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| EP2660336B1 (en) | 2008-06-11 | 2017-08-16 | Orion Pharma (UK) Limited | Isothermal nucleic acid amplification |
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2009
- 2009-06-11 EP EP13178545.3A patent/EP2660336B1/en active Active
- 2009-06-11 LT LTEP09762016.5T patent/LT2304054T/lt unknown
- 2009-06-11 DK DK09762016.5T patent/DK2304054T3/en active
- 2009-06-11 JP JP2011513055A patent/JP5798917B2/ja active Active
- 2009-06-11 HU HUE13178545A patent/HUE034561T2/en unknown
- 2009-06-11 PL PL09762016T patent/PL2304054T3/pl unknown
- 2009-06-11 EP EP17179338.3A patent/EP3249056B1/en active Active
- 2009-06-11 EP EP09762016.5A patent/EP2304054B1/en active Active
- 2009-06-11 CA CA2727212A patent/CA2727212C/en active Active
- 2009-06-11 US US12/997,382 patent/US9062344B2/en active Active
- 2009-06-11 SI SI200931718T patent/SI2304054T1/sl unknown
- 2009-06-11 HR HRP20171286TT patent/HRP20171286T1/hr unknown
- 2009-06-11 CN CN200980131223.9A patent/CN102119225B/zh active Active
- 2009-06-11 SI SI200931719T patent/SI2660336T1/sl unknown
- 2009-06-11 HU HUE09762016A patent/HUE036005T2/hu unknown
- 2009-06-11 PT PT97620165T patent/PT2304054T/pt unknown
- 2009-06-11 PL PL13178545T patent/PL2660336T3/pl unknown
- 2009-06-11 PT PT131785453T patent/PT2660336T/pt unknown
- 2009-06-11 CN CN201510103597.7A patent/CN104862385B/zh active Active
- 2009-06-11 WO PCT/GB2009/050662 patent/WO2009150467A1/en not_active Ceased
- 2009-06-11 DK DK13178545.3T patent/DK2660336T3/en active
- 2009-06-11 LT LTEP13178545.3T patent/LT2660336T/lt unknown
- 2009-06-11 ES ES09762016.5T patent/ES2638853T3/es active Active
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2010
- 2010-12-06 ZA ZA2010/08749A patent/ZA201008749B/en unknown
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2015
- 2015-05-12 US US14/709,793 patent/US9657340B2/en active Active
- 2015-08-21 JP JP2015164059A patent/JP6301881B2/ja active Active
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2017
- 2017-04-12 US US15/485,689 patent/US10472659B2/en active Active
- 2017-08-31 CY CY20171100921T patent/CY1119346T1/el unknown
- 2017-09-08 HR HRP20171356TT patent/HRP20171356T1/hr unknown
- 2017-09-11 CY CY20171100958T patent/CY1119331T1/el unknown
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