ES2647587T3 - Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo - Google Patents

Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo Download PDF

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Publication number
ES2647587T3
ES2647587T3 ES15152547.4T ES15152547T ES2647587T3 ES 2647587 T3 ES2647587 T3 ES 2647587T3 ES 15152547 T ES15152547 T ES 15152547T ES 2647587 T3 ES2647587 T3 ES 2647587T3
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Prior art keywords
aptamer
uridines
aptamers
benzyl group
thymidines
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Dominic Zichi
Sheri K. Wilcox
Chris Bock
Daniel J. Schneider
Bruce Eaton
Larry Gold
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Somalogic Inc
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Somalogic Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Un aptámero que se une a una molécula diana, en el que todos los restos de U y/o T de la región variable del aptámero están modificados químicamente en la posición 5, teniendo los nucleótidos modificados la siguiente estructura:**Fórmula** en la que Z >= al grupo de conexión R más (CH2)n, en la que n >= 1, 2 o 3, y además en la que R es:**Fórmula** y en donde el aptámero tiene una tasa de disociación (t1/2) de un complejo no covalente aptámero-molécula diana superior o igual a 30 minutos.

Description

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Claims (1)

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ES15152547.4T 2007-07-17 2008-07-17 Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo Active ES2647587T3 (es)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US95029307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US95028307P 2007-07-17 2007-07-17
US950293P 2007-07-17
US950283P 2007-07-17
US950281P 2007-07-17
US3142008P 2008-02-26 2008-02-26
US31420P 2008-02-26
US5159408P 2008-05-08 2008-05-08
US51594P 2008-05-08

Publications (1)

Publication Number Publication Date
ES2647587T3 true ES2647587T3 (es) 2017-12-22

Family

ID=40260081

Family Applications (6)

Application Number Title Priority Date Filing Date
ES17181688T Active ES2951068T3 (es) 2007-07-17 2008-07-17 Aptámero con una tasa de disociación mejorada, y complejo aptámero-diana no covalente del mismo
ES22162947T Active ES3045958T3 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates
ES15152547.4T Active ES2647587T3 (es) 2007-07-17 2008-07-17 Aptámeros con uridinas y/o timidinas sustituidas en la posición 5 con un grupo bencilo
ES12160299.9T Active ES2533711T3 (es) 2007-07-17 2008-07-17 Aptámeros con uridinas 5-(N-naftil)-sustituidas
ES09012809T Active ES2537322T5 (es) 2007-07-17 2008-07-17 Método para generar aptámeros con tasas de disociación mejoradas
ES08782013.0T Active ES2604764T3 (es) 2007-07-17 2008-07-17 Análisis de muestras de ensayo

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES17181688T Active ES2951068T3 (es) 2007-07-17 2008-07-17 Aptámero con una tasa de disociación mejorada, y complejo aptámero-diana no covalente del mismo
ES22162947T Active ES3045958T3 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES12160299.9T Active ES2533711T3 (es) 2007-07-17 2008-07-17 Aptámeros con uridinas 5-(N-naftil)-sustituidas
ES09012809T Active ES2537322T5 (es) 2007-07-17 2008-07-17 Método para generar aptámeros con tasas de disociación mejoradas
ES08782013.0T Active ES2604764T3 (es) 2007-07-17 2008-07-17 Análisis de muestras de ensayo

Country Status (17)

Country Link
US (1) US8409795B2 (es)
EP (10) EP2069529B1 (es)
JP (11) JP5684568B2 (es)
KR (3) KR101656240B1 (es)
CN (6) CN101802225B (es)
AU (3) AU2008276001A1 (es)
CA (4) CA2693453C (es)
DK (4) DK2489743T3 (es)
ES (6) ES2951068T3 (es)
FI (1) FI3284832T3 (es)
HR (1) HRP20150382T1 (es)
MX (7) MX2010000533A (es)
NO (1) NO2933340T3 (es)
PL (1) PL2489743T3 (es)
PT (2) PT2489743E (es)
SI (1) SI2489743T1 (es)
WO (3) WO2009012420A1 (es)

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JP2018078250A (ja) 2016-11-11 2018-05-17 株式会社ニューフレアテクノロジー マルチ荷電粒子ビーム描画装置

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