ES2650950T3 - Un método para filtrar una muestra biológica - Google Patents

Un método para filtrar una muestra biológica Download PDF

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Publication number
ES2650950T3
ES2650950T3 ES13740218.6T ES13740218T ES2650950T3 ES 2650950 T3 ES2650950 T3 ES 2650950T3 ES 13740218 T ES13740218 T ES 13740218T ES 2650950 T3 ES2650950 T3 ES 2650950T3
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Spain
Prior art keywords
filter
microorganism
sample
lysed sample
lysed
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ES13740218.6T
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English (en)
Inventor
Roel Penterman
Bart VAN MEERBERGEN
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Biocartis NV
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Biocartis NV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Un método para filtrar una muestra de sangre de mamífero, que comprende las etapas de: (a) proporcionar una muestra de sangre de mamífero con células sanguíneas, y que contiene o se sospecha que contiene un microorganismo; (b) llevar a cabo una lisis selectiva de las células sanguíneas para obtener una muestra lisada; (c) filtrar una parte de la muestra lisada obtenida en la etapa (b) a través de un filtro dispuesto para retener el microorganismo; (d) lavar el filtro con un tampón de lavado basado en detergente, capaz de solubilizar selectivamente sustancias diferentes al microorganismo retenido por el filtro, haciendo pasar el tampón de lavado basado en detergente a través del filtro, para retirar del filtro obstrucciones que consisten en las sustancias diferentes al microorganismo, para permitir una etapa adicional (c) de filtración de dicha muestra lisada; (e) repetir la etapa (c) al menos 1 vez, para filtrar al menos una parte adicional de la muestra lisada, y repetir opcionalmente las etapas (d) y (c) para que la muestra lisada pueda filtrarse completamente

Description

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Claims (1)

  1. imagen1
ES13740218.6T 2012-07-09 2013-06-26 Un método para filtrar una muestra biológica Active ES2650950T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12175581 2012-07-09
EP12175581.3A EP2684947A1 (en) 2012-07-09 2012-07-09 A method for filtering a biological sample
PCT/EP2013/063333 WO2014009151A1 (en) 2012-07-09 2013-06-26 A method for filtering a biological sample

Publications (1)

Publication Number Publication Date
ES2650950T3 true ES2650950T3 (es) 2018-01-23

Family

ID=48874250

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13740218.6T Active ES2650950T3 (es) 2012-07-09 2013-06-26 Un método para filtrar una muestra biológica

Country Status (7)

Country Link
US (1) US9663813B2 (es)
EP (3) EP2684947A1 (es)
JP (1) JP6250661B2 (es)
CN (1) CN104321423B (es)
CA (1) CA2871212A1 (es)
ES (1) ES2650950T3 (es)
WO (1) WO2014009151A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3588342B1 (en) * 2012-06-11 2022-10-12 Samsung Electronics Co., Ltd. Mobile device and control method thereof
KR102535489B1 (ko) 2014-06-13 2023-05-22 큐-리네아 에이비 미생물 검출 방법 및 특성 규명 방법
CA2961510A1 (en) * 2014-09-17 2016-03-24 Helixbind, Inc. Methods and devices for detecting and identifying microorganisms
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
JP6688561B2 (ja) * 2015-04-28 2020-04-28 デンカ生研株式会社 微生物抗原の回収法
CN115181781A (zh) * 2015-11-02 2022-10-14 拜奥法尔诊断有限责任公司 困难样品类型的样品制备
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
GB201617713D0 (en) * 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
CN109401975B (zh) * 2018-11-28 2021-08-03 云南中烟工业有限责任公司 一种收集陈化烟叶表面微生物的方法
JP7371245B2 (ja) * 2020-05-12 2023-10-30 株式会社日立ハイテク 自動分析装置、自動分析方法
CN111518161A (zh) * 2020-06-02 2020-08-11 英文特生物技术(北京)有限公司 一种柱式法从细胞中分离蛋白质的方法
CN114032280B (zh) * 2021-11-29 2023-06-23 北部湾大学 一种海洋无脊椎动物血细胞悬液的制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0531540B1 (en) * 1991-03-26 1996-07-10 Otsuka Pharmaceutical Factory, Inc. Method and device for filtering plasma
FR2824144B1 (fr) * 2001-04-30 2004-09-17 Metagenex S A R L Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel
ATE517192T1 (de) * 2006-08-10 2011-08-15 Merck Patent Gmbh Verfahren zur isolierung von zellen
JPWO2008152888A1 (ja) * 2007-06-11 2010-08-26 アサヒビール株式会社 微生物の精製方法及び微生物の検出方法
JP2010535016A (ja) * 2007-08-02 2010-11-18 ユニヴェルシテ・ラヴァル 体液から微生物細胞および微生物核酸の濃縮および富化
DE102007041864B4 (de) * 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
JP2009095307A (ja) * 2007-10-18 2009-05-07 Olympus Corp 細胞処理容器および細胞処理装置
EP2333105A1 (en) 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
CA2831969A1 (en) * 2011-06-06 2012-12-30 Biocartis S.A. Selective lysis of cells by ionic surfactants

Also Published As

Publication number Publication date
EP2684947A1 (en) 2014-01-15
EP3290509A1 (en) 2018-03-07
CN104321423A (zh) 2015-01-28
CN104321423B (zh) 2018-09-25
EP2870233A1 (en) 2015-05-13
JP2015527063A (ja) 2015-09-17
US9663813B2 (en) 2017-05-30
US20150132793A1 (en) 2015-05-14
JP6250661B2 (ja) 2017-12-20
CA2871212A1 (en) 2014-01-16
WO2014009151A1 (en) 2014-01-16
EP2870233B1 (en) 2017-11-08

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