ES2653642T3 - Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO - Google Patents

Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO Download PDF

Info

Publication number
ES2653642T3
ES2653642T3 ES13868457.6T ES13868457T ES2653642T3 ES 2653642 T3 ES2653642 T3 ES 2653642T3 ES 13868457 T ES13868457 T ES 13868457T ES 2653642 T3 ES2653642 T3 ES 2653642T3
Authority
ES
Spain
Prior art keywords
cto
nucleic acid
pto
target nucleic
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13868457.6T
Other languages
English (en)
Spanish (es)
Inventor
Jong Yoon Chun
Young Jo Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seegene Inc
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Application granted granted Critical
Publication of ES2653642T3 publication Critical patent/ES2653642T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES13868457.6T 2012-12-27 2013-12-27 Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO Active ES2653642T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
KR20120154834 2012-12-27
KR20120154834 2012-12-27
KR20130008580 2013-01-25
KR20130008580 2013-01-25
KR20130034670 2013-03-29
KR20130034670 2013-03-29
PCT/KR2013/012312 WO2014104818A1 (en) 2012-12-27 2013-12-27 Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay

Publications (1)

Publication Number Publication Date
ES2653642T3 true ES2653642T3 (es) 2018-02-08

Family

ID=51021756

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13868457.6T Active ES2653642T3 (es) 2012-12-27 2013-12-27 Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO

Country Status (12)

Country Link
US (2) US9783845B2 (2)
EP (1) EP2943586B1 (2)
JP (1) JP6178430B2 (2)
KR (1) KR101760267B1 (2)
CN (1) CN104903465B (2)
AU (1) AU2013371128B2 (2)
BR (1) BR112015014152B1 (2)
CA (1) CA2896213C (2)
ES (1) ES2653642T3 (2)
MX (1) MX361191B (2)
RU (1) RU2620958C2 (2)
WO (1) WO2014104818A1 (2)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017213458A1 (en) 2016-06-10 2017-12-14 Seegene, Inc. Methods for preparing tagging oligonucleotides
KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
EP3523452B1 (en) 2016-10-06 2026-04-15 Seegene, Inc. Methods for preparing oligonucleotides for detecting target nucleic acid molecules in samples
CA3049833A1 (en) * 2016-12-23 2018-06-28 Albert-Ludwigs-Universitat Freiburg Two-piece mediator probe
IL319189A (en) 2016-12-29 2025-04-01 Seegene Inc Method for reducing primer dimer formation and increasing amplification efficiency
CN110475871B (zh) 2017-03-28 2024-04-12 Seegene株式会社 用于确定靶核酸序列的存在的分析信号
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
EP3735476B1 (en) 2018-01-05 2023-08-23 Seegene, Inc. Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
KR102464501B1 (ko) 2018-04-20 2022-11-17 주식회사 씨젠 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치
WO2020122679A1 (en) 2018-12-14 2020-06-18 Seegene, Inc. Method for detecting a target analyte in a sample using an s-shaped function for a slope data set
US20220298555A1 (en) * 2018-12-20 2022-09-22 Roche Molecular Systems, Inc. Detection of target nucleic acid by solid-phase molography
EP4301880A4 (en) 2021-03-05 2025-06-18 Seegene, Inc. METHOD FOR DETECTING SARS-COV-2 MUTATIONS
KR20240058112A (ko) * 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
EP4265734A1 (de) * 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
US20250340928A1 (en) * 2022-04-22 2025-11-06 Albert-Ludwigs-Universität Freiburg Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex
KR20250086619A (ko) 2022-10-14 2025-06-13 주식회사 씨젠 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법
EP4372100A1 (en) * 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
EP4621068A1 (en) 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
WO2025005717A1 (ko) * 2023-06-30 2025-01-02 주식회사 씨젠 Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7309573B2 (en) 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
KR20020063359A (ko) 2001-01-27 2002-08-03 일렉트론 바이오 (주) 핵산 및 올리거뉴클레오티드의 상보적 이중결합의 특정서열에 특이적으로 반응하는 절단기법을 이용한 핵산 혼성분석 방법 및 장치
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
EP1689764B1 (en) 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Oligonucleotides labeled with a plurality of fluorophores
EP2046989B1 (en) * 2006-07-17 2013-03-20 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
US20090023151A1 (en) * 2006-08-30 2009-01-22 Dawson Elliott P Method For The Labeling And Detection Of Small Polynucleotides
WO2008083261A1 (en) 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
MX352460B (es) * 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
JP5852222B2 (ja) * 2011-03-29 2016-02-03 シージーン アイエヌシー Pto切断及び延長−依存的切断によるターゲット核酸配列の検出
MX342067B (es) * 2011-05-04 2016-09-09 Seegene Inc Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda.

Also Published As

Publication number Publication date
EP2943586A4 (en) 2016-09-28
WO2014104818A1 (en) 2014-07-03
KR101760267B1 (ko) 2017-07-24
CN104903465B (zh) 2017-04-26
EP2943586B1 (en) 2017-09-20
US20160060690A1 (en) 2016-03-03
JP6178430B2 (ja) 2017-08-09
CA2896213C (en) 2018-12-11
EP2943586A1 (en) 2015-11-18
JP2016502861A (ja) 2016-02-01
MX2015008494A (es) 2015-09-10
AU2013371128A1 (en) 2015-08-13
US9783845B2 (en) 2017-10-10
BR112015014152A2 (2) 2017-08-22
KR20150093766A (ko) 2015-08-18
RU2015130335A (ru) 2017-01-30
RU2620958C2 (ru) 2017-05-30
US20180057869A1 (en) 2018-03-01
MX361191B (es) 2018-11-29
AU2013371128B2 (en) 2017-08-10
CN104903465A (zh) 2015-09-09
BR112015014152B1 (pt) 2023-01-24
CA2896213A1 (en) 2014-07-03

Similar Documents

Publication Publication Date Title
ES2653642T3 (es) Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO
US10731203B2 (en) Detection of target nucleic acid sequence by PTO cleavage and extension-dependent signaling oligonucleotide hybridization assay
ES2629759T3 (es) Detección de secuencias de ácidos nucleicos diana mediante un ensayo de escisión y extensión de PTO
ES2657906T3 (es) Detección de secuencias de ácidos nucleicos objetivo mediante una escisión y una hibridación de un PO
ES2669244T3 (es) Detección de variación de nucleótidos en una secuencia de ácido nucleico diana mediante escisión CTO y ensayo de extensión
JP6096885B2 (ja) PTO切断及び伸長依存的なシグナリングオリゴヌクレオチド切断を用いたターゲット核酸配列の検出{DetectionofTargetNucleicAcidSequencebyPTOCleavageandExtension−DependentSignalingOligonucleotideCleavage}
US20160258007A1 (en) Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay
AU2015203677B2 (en) Detection of Target Nucleic Acid Sequences by PTO Cleavage and Extension
RU2588457C2 (ru) Определение целевых нуклеиновокислотных последовательностей путем расщепления зондирующего олигонуклеотида и гибридизации
WO2013187628A1 (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent transcription