ES2663386T3 - Novirabdovirus recombinante utilizable como vector de antígenos - Google Patents

Novirabdovirus recombinante utilizable como vector de antígenos Download PDF

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Publication number
ES2663386T3
ES2663386T3 ES13799108.9T ES13799108T ES2663386T3 ES 2663386 T3 ES2663386 T3 ES 2663386T3 ES 13799108 T ES13799108 T ES 13799108T ES 2663386 T3 ES2663386 T3 ES 2663386T3
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Prior art keywords
protein
domain
novirabdovirus
recombinant
rabdovirus
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ES13799108.9T
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English (en)
Inventor
Michel Bremont
Angella NZONZA
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Institut National de la Recherche Agronomique
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Institut National de la Recherche Agronomique
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/205—Rhabdoviridae, e.g. rabies virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20041—Use of virus, viral particle or viral elements as a vector
    • C12N2760/20043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Novirabdovirus recombinante cuyo genoma contiene, además de los genes que codifican las proteínas N, P, M, G y L endógenas de dicho novirabdovirus, un gen exógeno que codifica una proteína quimérica, caracterizándose dicha proteína quimérica porque comprende la secuencia de una proteína antigénica de interés, no siendo dicha proteína antigénica de interés una glicoproteína, fusionada en su extremo N terminal con el péptido señal de la proteína G de un rabdovirus y en su extremo C-terminal con un fragmento de secuencia de una proteína G de rabdovirus, comprendiendo dicho fragmento el dominio transmembrana de dicha proteína G de rabdovirus o una parte de ésta que comprende al menos 15 aminoácidos C-terminales consecutivos de dicho dominio, caracterizado porque comprende además, a continuación del dominio transmembrana o de la parte de éste, el dominio intravirión de una proteína G de rabdovirus, o una parte de dicho dominio intravirión, que comprende al menos 3 aminoácidos N-terminales consecutivos de dicho dominio.

Description

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Claims (1)

  1. imagen1
ES13799108.9T 2012-10-15 2013-10-08 Novirabdovirus recombinante utilizable como vector de antígenos Active ES2663386T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1259815A FR2996856B1 (fr) 2012-10-15 2012-10-15 Novirhabdovirus recombinant utilisable comme vecteur d'antigenes
FR1259815 2012-10-15
PCT/IB2013/059207 WO2014060905A1 (fr) 2012-10-15 2013-10-08 Novirhabdovirus recombinant utilisable comme vecteur d'antigènes

Publications (1)

Publication Number Publication Date
ES2663386T3 true ES2663386T3 (es) 2018-04-12

Family

ID=48224842

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13799108.9T Active ES2663386T3 (es) 2012-10-15 2013-10-08 Novirabdovirus recombinante utilizable como vector de antígenos

Country Status (5)

Country Link
US (1) US9907843B2 (es)
EP (1) EP2906704B1 (es)
ES (1) ES2663386T3 (es)
FR (1) FR2996856B1 (es)
WO (1) WO2014060905A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109136200B (zh) * 2018-09-20 2022-02-18 中国水产科学研究院黑龙江水产研究所 一种重组传染性造血器官坏死病毒及其构建方法与应用
WO2023214082A2 (en) * 2022-05-06 2023-11-09 Sanofi Signal sequences for nucleic acid vaccines
CN116375886A (zh) * 2023-05-09 2023-07-04 郑州市第六人民医院(郑州市结核病防治所) 一种诺如病毒vp1融合蛋白及表达方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL136905A0 (en) * 1997-12-22 2001-06-14 Univ Tennessee Res Corp Recombinant rhabdovirus containing a heterologous fusion protein
FR2839453B1 (fr) 2002-05-07 2007-05-11 Agronomique Inst Nat Rech Utilisation de novirhabdovirus modifies pour l'obtention de vaccins
JP4578973B2 (ja) * 2002-09-17 2010-11-10 ノバルティス アーゲー 免疫刺激のためのｉｈｎｖｇ蛋白質
EP1867725A1 (en) * 2006-06-16 2007-12-19 Institut National De La Recherche Agronomique (Inra) Recombinant novirhabdoviruses and uses thereof
ES2708856T3 (es) * 2007-08-03 2019-04-11 Pasteur Institut Vectores de transferencia de gen lentivírico y sus aplicaciones medicinales

Also Published As

Publication number Publication date
FR2996856B1 (fr) 2015-06-05
EP2906704B1 (fr) 2017-12-27
EP2906704A1 (fr) 2015-08-19
FR2996856A1 (fr) 2014-04-18
US20150265699A1 (en) 2015-09-24
US9907843B2 (en) 2018-03-06
WO2014060905A1 (fr) 2014-04-24

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