ES2664227T3 - Análisis celular de fluidos corporales - Google Patents

Análisis celular de fluidos corporales Download PDF

Info

Publication number
ES2664227T3
ES2664227T3 ES12862848.4T ES12862848T ES2664227T3 ES 2664227 T3 ES2664227 T3 ES 2664227T3 ES 12862848 T ES12862848 T ES 12862848T ES 2664227 T3 ES2664227 T3 ES 2664227T3
Authority
ES
Spain
Prior art keywords
fluid
cells
body fluid
fluorescent dye
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES12862848.4T
Other languages
English (en)
Inventor
Jiong Wu
Emily H. LIN
Jihping YANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2664227T3 publication Critical patent/ES2664227T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Un método in vitro para analizar un fluido corporal que contiene células, en que dicho método comprende: teñir un fluido corporal que comprende menos de 100 células/μl y seleccionado entre líquido cefalorraquídeo, líquido peritoneal, líquido pericárdico, líquido pleural, líquido sinovial, orina, saliva, lágrimas, semen, líquido amniótico, esputo y líquido ascítico, con un colorante fluorescente, en el que el colorante fluorescente está presente en una cantidad entre 0,000001 y 0,5 % (p/v), penetra en una membrana celular y se une a un ácido nucleico para formar un complejo colorante en el interior de la célula; irradiar el fluido corporal teñido con energía procedente de una fuente de energía; medir una señal de fluorescencia emitida por el complejo colorante en el fluido corporal teñido; y diferenciar células con núcleos de células sin núcleos, basándose en la presencia o ausencia del colorante fluorescente.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8

Claims (1)

  1. imagen1
ES12862848.4T 2011-12-27 2012-12-19 Análisis celular de fluidos corporales Active ES2664227T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161580623P 2011-12-27 2011-12-27
US201161580623P 2011-12-27
PCT/US2012/070696 WO2013101603A1 (en) 2011-12-27 2012-12-19 Cellular analysis of body fluids

Publications (1)

Publication Number Publication Date
ES2664227T3 true ES2664227T3 (es) 2018-04-18

Family

ID=48654915

Family Applications (2)

Application Number Title Priority Date Filing Date
ES18153846T Active ES2895391T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales
ES12862848.4T Active ES2664227T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES18153846T Active ES2895391T3 (es) 2011-12-27 2012-12-19 Análisis celular de fluidos corporales

Country Status (5)

Country Link
US (3) US9970045B2 (es)
EP (2) EP2798087B1 (es)
CN (2) CN108279301A (es)
ES (2) ES2895391T3 (es)
WO (1) WO2013101603A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
US8986944B2 (en) * 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
EP2041299A4 (en) * 2006-07-14 2010-01-13 Aviva Biosciences Corp METHOD AND COMPOSITIONS FOR DETECTION OF RARE CELLS FROM A BIOLOGICAL SAMPLE
ES2895391T3 (es) * 2011-12-27 2022-02-21 Abbott Lab Análisis celular de fluidos corporales
WO2015168393A1 (en) * 2014-04-30 2015-11-05 Northwestern University Nanostructures for modulating intercellular communication and uses thereof
US10413565B2 (en) 2014-04-30 2019-09-17 Northwestern University Nanostructures for modulating intercellular communication and uses thereof
US11193927B2 (en) 2016-09-08 2021-12-07 Abbott Laboratories Automated body fluid analysis
CN107686859B (zh) * 2017-07-18 2021-07-30 天津师范大学 一种适用于鱼类血细胞快速分类计数的方法及应用
JP7144511B2 (ja) * 2017-09-05 2022-09-29 ベックマン コールター， インコーポレイテッド 診療現場診断のための血液サンプルの収集および調製
SG11202008344WA (en) * 2018-03-01 2020-09-29 Ecolab Usa Inc Method of measuring benzimidazole-based compounds in water
EP3837543A4 (en) * 2018-08-16 2022-11-16 Essenlix Corporation CELL ANALYSIS IN BODY FLUID, ESPECIALLY BLOOD
JP2022544851A (ja) * 2019-08-22 2022-10-21 マイケル・イアノッティ ハイスループット分析及びソーティング、並びにハイスループット分析及びソーティングためのサンプリングインターフェース及びアセンブリ
CN114450589A (zh) * 2019-10-23 2022-05-06 深圳迈瑞生物医疗电子股份有限公司 分析血液样本中红细胞方法及血液分析系统
CN113567408A (zh) * 2021-07-28 2021-10-29 上海睿钰生物科技有限公司 一种确定荧光通道曝光时间的方法
CN116067742B (zh) * 2023-03-29 2023-09-01 北京汉氏联合生物技术股份有限公司 CTCs染色试剂盒和染色方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4325706A (en) * 1980-08-15 1982-04-20 Ortho Diagnostic Systems Inc. Automated detection of platelets and reticulocytes in whole blood
US4485175A (en) * 1983-01-03 1984-11-27 Coulter Electronics, Inc. Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
JP2549665B2 (ja) * 1987-07-31 1996-10-30 東亜医用電子株式会社 フロ−サイトメトリ用網状赤血球測定試薬
IE76319B1 (en) 1990-07-23 1997-10-22 Becton Dickinson Co Preservation of cells as controls or standards in cellular analysis
JP3070968B2 (ja) * 1991-05-14 2000-07-31 シスメックス株式会社 尿中の細胞分析用試薬及び方法
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5812419A (en) 1994-08-01 1998-09-22 Abbott Laboratories Fully automated analysis method with optical system for blood cell analyzer
US5691204A (en) * 1995-04-21 1997-11-25 Abbott Laboratories Compositions and methods for the rapid analysis of reticulocytes
US5686308A (en) * 1995-06-08 1997-11-11 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US6911313B2 (en) 1998-02-06 2005-06-28 Sysmex Corporation Process for discriminating and counting erythroblasts
ATE399878T1 (de) * 1999-10-25 2008-07-15 Genprime Inc Verfahren und vorrichtung zur quantifizierung von prokaryotischen und eukaryotischen zellen
CA2428740A1 (en) 2002-05-20 2003-11-20 Bayer Corporation Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf)
US7625712B2 (en) * 2004-05-21 2009-12-01 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
JP4976038B2 (ja) * 2006-03-29 2012-07-18 シスメックス株式会社 血液学的試料の測定方法
JP4806334B2 (ja) * 2006-11-27 2011-11-02 シスメックス株式会社 血液学的試料の測定方法および測定装置
EP3260842B1 (en) 2007-02-01 2022-04-13 Sysmex Corporation Hematological analyzer and method for analyzing a sample
US9602777B2 (en) 2008-04-25 2017-03-21 Roche Diagnostics Hematology, Inc. Systems and methods for analyzing body fluids
JP5452058B2 (ja) 2009-03-31 2014-03-26 シスメックス株式会社 血液分析装置
US9097704B2 (en) * 2010-05-05 2015-08-04 Abbott Laboratories Method for hematology analysis
ES2902648T3 (es) * 2011-05-04 2022-03-29 Abbott Lab Método de análisis de glóbulos rojos nucleados y analizador hematológico automatizado
ES2927316T3 (es) * 2011-05-04 2022-11-04 Abbott Lab Sistema y método de análisis de glóbulos blancos
ES2895391T3 (es) * 2011-12-27 2022-02-21 Abbott Lab Análisis celular de fluidos corporales

Also Published As

Publication number Publication date
WO2013101603A1 (en) 2013-07-04
EP2798087B1 (en) 2018-03-07
EP3382035B1 (en) 2021-08-11
CN108279301A (zh) 2018-07-13
US20130164740A1 (en) 2013-06-27
US20210130866A1 (en) 2021-05-06
CN104024437A (zh) 2014-09-03
EP2798087A4 (en) 2015-06-24
US20180334699A1 (en) 2018-11-22
ES2895391T3 (es) 2022-02-21
EP2798087A1 (en) 2014-11-05
US10774359B2 (en) 2020-09-15
EP3382035A1 (en) 2018-10-03
US9970045B2 (en) 2018-05-15

Similar Documents

Publication Publication Date Title
ES2664227T3 (es) Análisis celular de fluidos corporales
ES2548133T3 (es) Método de detección de niveles muy bajos de analito en una muestra fluida de película delgada contenida en una cámara de pequeño espesor
WO2010101926A3 (en) Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption
BR112014006432A2 (pt) biomarcadores do câncer de pulmão e seus usos
WO2012151103A3 (en) Nucleated red blood cell analysis system and method
CO6231057A2 (es) Sensibilidad mejorada de determinacion del perfil de anticuerpos a traves de una colocacion en capas incrementada de anticuerpo reportero
MX2013001042A (es) Biomarcadores de cancer pancreatico y usos de los mismos.
BR112015020370A2 (pt) dispositivo de análise de espécimes de urina e método de análise de espécimes de urina
BR112015001075A8 (pt) Centrífuga compacta de alta velocidade para uso com recipientes de amostra de baixo volume, método e dispositivo
BR122021002358B8 (pt) Sistema fluídico
MX367366B (es) Composiciones y metodos para diagnosticar tumores de tiroides.
WO2012151105A3 (en) Basophil analysis system and method
BR112013027349A2 (pt) método para classificação/contagem de leucócitos, kit de reagentes para classificação de leucócitos, e reagentes para classificação de leucócitos
Cho et al. Fluorescence-based detection and quantification of features of cellular senescence
BR112015027553A2 (pt) cartucho de imageamento, pipeta e método de uso para microscopia de esfregaço de expectoração direta
PE20160657A1 (es) Composiciones y metodos para identificar un riesgo de cancer en un sujeto
CN105849559B (zh) 用于检测循环肿瘤细胞(ctcs)的方法
IN2014DN08957A (es)
MX2018000679A (es) Prueba mejorada de endotoxina bacteriana para determinacion de endotoxinas.
AR053948A1 (es) Deteccion de un antigeno diana independientemente de la presencia o ausencia de un anticuerpo terapeutico correspondiente
BR112014031958A2 (pt) bag3 como marcador de tecido e soro bioquímico
BR112018017304A2 (pt) cartucho para purificar uma amostra e análise
ES2572630T3 (es) Procedimiento para el enriquecimiento de ácidos nucleicos de cadena corta
WO2008123180A1 (ja) 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット
MX2013010997A (es) Ensayo para clasificacion de compuestos que disminuyen selectivamente el numero de celulas madre de cance.