ES2767728T3 - Célula eucariota con elevada producción de producto de fermentación - Google Patents

Célula eucariota con elevada producción de producto de fermentación Download PDF

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Publication number: ES2767728T3
Authority: ES; Spain
Prior art keywords: eukaryotic cell; dehydrogenase; glucose; fermentation; nad
Prior art date: 2015-10-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

Application number

ES16778760T

Other languages

English (en)

Spanish (es)

Inventor

Ioannis Papapetridis

Jacobus Pronk

Maris Antonius Van

Paul Klaassen

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

DSM IP Assets BV

Original Assignee

DSM IP Assets BV

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2015-10-06

Filing date

2016-10-03

Publication date

2020-06-18

2016-10-03 Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV

2020-06-18 Application granted granted Critical

2020-06-18 Publication of ES2767728T3 publication Critical patent/ES2767728T3/es

Status Active legal-status Critical Current

2036-10-03 Anticipated expiration legal-status Critical

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230000002588 toxic effect Effects 0.000 description 1
QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
238000000844 transformation Methods 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
230000003827 upregulation Effects 0.000 description 1
238000010200 validation analysis Methods 0.000 description 1
MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
235000012141 vanillin Nutrition 0.000 description 1
235000013311 vegetables Nutrition 0.000 description 1
238000011514 vinification Methods 0.000 description 1
150000003722 vitamin derivatives Chemical class 0.000 description 1
235000020234 walnut Nutrition 0.000 description 1
239000002916 wood waste Substances 0.000 description 1
101150011516 xlnD gene Proteins 0.000 description 1
101150034227 xyl1 gene Proteins 0.000 description 1
229920001221 xylan Polymers 0.000 description 1
150000003741 xylose derivatives Chemical class 0.000 description 1
108091022915 xylulokinase Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C12N15/09—Recombinant DNA-technology
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C12N9/93—Ligases (6)
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- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01044—Phosphogluconate dehydrogenase (decarboxylating) (1.1.1.44)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
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- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)
- C12Y101/05003—Glycerol-3-phosphate dehydrogenase (1.1.5.3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03021—Glycerol-1-phosphatase (3.1.3.21)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01005—Xylose isomerase (5.3.1.5)
- C—CHEMISTRY; METALLURGY
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- C12Y602/01—Acid-Thiol Ligases (6.2.1)
- C12Y602/0101—Acid--CoA ligase (GDP-forming) (6.2.1.10)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

ES16778760T 2015-10-06 2016-10-03 Célula eucariota con elevada producción de producto de fermentación Active ES2767728T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP15188645		2015-10-06
PCT/EP2016/073561 WO2017060195A1 (en)	2015-10-06	2016-10-03	Eukaryotic cell with increased production of fermentation product

Publications (1)

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ES2767728T3 true ES2767728T3 (es)	2020-06-18

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US (1)	US11136600B2 (pl)
EP (2)	EP3620516A1 (pl)
CN (1)	CN108603179B (pl)
BR (1)	BR112018004548A2 (pl)
CA (1)	CA2998981C (pl)
DK (1)	DK3359655T3 (pl)
ES (1)	ES2767728T3 (pl)
MY (1)	MY186400A (pl)
PL (1)	PL3359655T3 (pl)
WO (1)	WO2017060195A1 (pl)

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DK3559246T3 (da) *	2016-12-20	2022-09-05	Dsm Ip Assets Bv	Forbedrede gærstammer til ethanolproduktion
DK3679059T3 (da) *	2017-09-04	2022-02-21	Metgen Oy	Nye forbedrede glucoseisomeraser
CA3089799A1 (en) *	2018-02-01	2019-08-08	Dsm Ip Assets B.V.	Yeast cell capable of simultaneously fermenting hexose and pentose sugars
CN113913448B (zh) *	2021-07-23	2023-10-20	中国人民解放军军事科学院军事医学研究院	一种提高甲基营养菌吡咯喹啉醌产量的方法及应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1990014423A1 (en)	1989-05-18	1990-11-29	The Infergene Company	Microorganism transformation
WO1991000920A2 (en)	1989-07-07	1991-01-24	Unilever N.V.	Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
DE69432543T2 (de)	1993-07-23	2003-12-24	Dsm N.V., Te Heerlen	Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
JP5303084B2 (ja)	1997-04-11	2013-10-02	コニンクリーケデーエスエムナムローゼフェンノートシャップ	工業的組み換え生物を溝築するための手段としての遺伝子変換
US6265186B1 (en)	1997-04-11	2001-07-24	Dsm N.V.	Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
CN100390285C (zh)	1998-05-19	2008-05-28	Dsm公司	体内生产头孢菌素的改良
CN1331751A (zh)	1998-12-22	2002-01-16	Dsm公司	改进的头孢菌素的体内生产方法
US7253001B2 (en) *	2002-03-19	2007-08-07	Forskarpatent I Syd Ab	Metabolic engineering for improved xylose utilisation of Saccharomyces cerevisiae
SE0202090D0 (sv)	2002-05-08	2002-07-04	Forskarpatent I Syd Ab	A modifierd yeast consuming L-arabinose
EA016303B1 (ru)	2006-10-02	2012-04-30	ДСМ АйПи АССЕТС Б.В.	Метаболическая инженерия сбраживающих арабинозу дрожжевых клеток
WO2009013159A2 (en)	2007-07-23	2009-01-29	Dsm Ip Assets B.V.	Acetyl-coa producing enzymes in yeast
EA201001280A1 (ru)	2008-03-13	2011-02-28	ДСМ АйПи АССЕТС Б.В.	Селекция организмов, способных сбраживать смешанные субстраты
EP2277989A1 (en)	2009-07-24	2011-01-26	Technische Universiteit Delft	Fermentative glycerol-free ethanol production
MX2014002787A (es) *	2011-09-08	2014-12-08	Genomatica Inc	Organismos eucarioticos y metodos para producir 1, 3 -butanodiol.
CN102978148B (zh) *	2012-12-18	2014-08-06	南京工业大学	一株氧化葡糖酸杆菌工程菌及其构建方法和在制备木糖醇中的应用
AR097479A1 (es)	2013-08-29	2016-03-16	Dsm Ip Assets Bv	Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado
AR097480A1 (es)	2013-08-29	2016-03-16	Dsm Ip Assets Bv	Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
EP3122876B1 (en)	2014-03-28	2020-11-25	Danisco US Inc.	Altered host cell pathway for improved ethanol production

2016
- 2016-10-03 WO PCT/EP2016/073561 patent/WO2017060195A1/en not_active Ceased
- 2016-10-03 PL PL16778760T patent/PL3359655T3/pl unknown
- 2016-10-03 ES ES16778760T patent/ES2767728T3/es active Active
- 2016-10-03 CN CN201680054678.5A patent/CN108603179B/zh not_active Expired - Fee Related
- 2016-10-03 US US15/766,184 patent/US11136600B2/en active Active
- 2016-10-03 DK DK16778760.5T patent/DK3359655T3/da active
- 2016-10-03 BR BR112018004548A patent/BR112018004548A2/pt not_active Application Discontinuation
- 2016-10-03 MY MYPI2018700875A patent/MY186400A/en unknown
- 2016-10-03 EP EP19198880.7A patent/EP3620516A1/en not_active Withdrawn
- 2016-10-03 CA CA2998981A patent/CA2998981C/en active Active
- 2016-10-03 EP EP16778760.5A patent/EP3359655B1/en active Active

Also Published As

Publication number	Publication date
US20180291404A1 (en)	2018-10-11
CA2998981A1 (en)	2017-04-13
CN108603179B (zh)	2022-02-08
BR112018004548A2 (pt)	2019-09-17
EP3620516A1 (en)	2020-03-11
DK3359655T3 (da)	2020-02-03
WO2017060195A1 (en)	2017-04-13
PL3359655T3 (pl)	2020-05-18
CN108603179A (zh)	2018-09-28
MY186400A (en)	2021-07-22
EP3359655B1 (en)	2019-11-20
EP3359655A1 (en)	2018-08-15
CA2998981C (en)	2022-04-26
US11136600B2 (en)	2021-10-05

Publication	Publication Date	Title
Jin et al.	2017	Metabolic engineering of yeast for lignocellulosic biofuel production
US12012604B2 (en)	2024-06-18	Acetate consuming yeast cell
WO2018172328A1 (en)	2018-09-27	Improved glycerol free ethanol production
CN118325748A (zh)	2024-07-12	重组酵母细胞
ES2767728T3 (es)	2020-06-18	Célula eucariota con elevada producción de producto de fermentación
US11198847B2 (en)	2021-12-14	Yeast cell capable of simultaneously fermenting hexose and pentose sugars
Dmytruk et al.	2013	Metabolic engineering of the yeast Hansenula polymorpha for the construction of efficient ethanol producers
WO2019110492A1 (en)	2019-06-13	Recombinant yeast cell
Dmytruk et al.	2017	Development of the thermotolerant methylotrophic yeast Hansenula polymorpha as efficient ethanol producer
EP4370692A1 (en)	2024-05-22	Recombinant yeast cell
EP4370651A1 (en)	2024-05-22	Recombinant yeast cell
CN117940571A (zh)	2024-04-26	重组酵母细胞
CN117881773A (zh)	2024-04-12	重组酵母细胞
WO2019110491A1 (en)	2019-06-13	Recombinant yeast cell
CN117916381A (zh)	2024-04-19	重组酵母细胞
CA3045740C (en)	2025-12-09	Improved glycerol free ethanol production
US20250320529A1 (en)	2025-10-16	Recombinant yeast cell
WO2023285282A1 (en)	2023-01-19	Recombinant yeast cell
WO2023285280A1 (en)	2023-01-19	Recombinant yeast cell
WO2023208762A2 (en)	2023-11-02	Mutant yeast cell and process for the production of ethanol
BR112018074490B1 (pt)	2025-06-03	Célula de levedura recombinante e processo para preparar um composto orgânico
배이현	2014	Investigation of mixed sugar fermentation for production of ethanol by engineered Saccharomyces cerevisiae
WO2018073107A1 (en)	2018-04-26	Eukaryotic cell comprising xylose isomerase