ES2910923T3 - Dispositivo y método para la separación de fluidos por centrifugación en gradiente de densidad - Google Patents
Dispositivo y método para la separación de fluidos por centrifugación en gradiente de densidad Download PDFInfo
- Publication number
- ES2910923T3 ES2910923T3 ES16791676T ES16791676T ES2910923T3 ES 2910923 T3 ES2910923 T3 ES 2910923T3 ES 16791676 T ES16791676 T ES 16791676T ES 16791676 T ES16791676 T ES 16791676T ES 2910923 T3 ES2910923 T3 ES 2910923T3
- Authority
- ES
- Spain
- Prior art keywords
- partition
- container
- insert
- separation
- fractions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 52
- 238000000432 density-gradient centrifugation Methods 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims description 27
- 239000012530 fluid Substances 0.000 title description 24
- 238000005192 partition Methods 0.000 claims abstract description 70
- 239000007788 liquid Substances 0.000 claims abstract description 54
- 238000005119 centrifugation Methods 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 239000013060 biological fluid Substances 0.000 claims abstract description 11
- 239000000725 suspension Substances 0.000 claims abstract description 6
- 210000004369 blood Anatomy 0.000 claims description 31
- 239000008280 blood Substances 0.000 claims description 31
- 210000003743 erythrocyte Anatomy 0.000 claims description 12
- 210000000265 leukocyte Anatomy 0.000 claims description 11
- 239000000523 sample Substances 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 8
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Centrifugal Separators (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- External Artificial Organs (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL413910A PL237582B1 (pl) | 2015-09-15 | 2015-09-15 | Insert do pojemnika do wirowania, zwłaszcza, do probówki, do wydzielenia frakcji o pożądanym zakresie gęstości metodą wirowania na gradiencie gęstości oraz pojemnik do wirowania zawierający ten insert |
| PCT/IB2016/055503 WO2017046736A1 (en) | 2015-09-15 | 2016-09-15 | Device and method for fluids separation by density gradient |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2910923T3 true ES2910923T3 (es) | 2022-05-17 |
Family
ID=58360192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES16791676T Active ES2910923T3 (es) | 2015-09-15 | 2016-09-15 | Dispositivo y método para la separación de fluidos por centrifugación en gradiente de densidad |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP3349897B8 (de) |
| CN (1) | CN108367288A (de) |
| DK (1) | DK3349897T3 (de) |
| ES (1) | ES2910923T3 (de) |
| PL (1) | PL237582B1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113329809B (zh) * | 2018-12-08 | 2023-01-31 | 魏民 | 用于生产细胞疗法产品的装置 |
| TWI683702B (zh) * | 2019-01-31 | 2020-02-01 | 瑩芳有限公司 | 採檢容器 |
| SE545603C2 (en) * | 2019-08-22 | 2023-11-07 | Grimaldi Dev Ab | Separating particles through centrifugal sedimentation |
| PL434517A1 (pl) * | 2020-06-30 | 2022-01-03 | Spark-Tech Spółka Z Ograniczoną Odpowiedzialnością | Urządzenie do kontrolowanego warstwowego nalewania cieczy |
| CN113358451A (zh) * | 2021-03-30 | 2021-09-07 | 刘开 | 一种新型离心层析提取器 |
| CN114053986B (zh) * | 2021-12-09 | 2024-12-10 | 杭州职业技术学院 | 可离心控制顺序混合反应的试剂预封装容器 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5132232A (en) * | 1985-07-30 | 1992-07-21 | V-Tech, Inc. | Method and apparatus for preparation of liquids for examination |
| IL100828A (en) * | 1992-01-31 | 2002-05-23 | Novamed Ltd | Method and means for density gradient centrifugation |
| US5648223A (en) * | 1994-08-31 | 1997-07-15 | Activated Cell Therapy, Inc. | Methods for enriching breast tumor cells |
| US6516953B1 (en) * | 1998-12-05 | 2003-02-11 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
| US7074577B2 (en) * | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
| JP4853295B2 (ja) * | 2007-01-11 | 2012-01-11 | 株式会社島津製作所 | 遠心分離機の遠沈管 |
| WO2012149641A1 (en) * | 2011-05-05 | 2012-11-08 | Stemcell Technologies Inc. | Method and insert for density gradient separation |
-
2015
- 2015-09-15 PL PL413910A patent/PL237582B1/pl unknown
-
2016
- 2016-09-15 DK DK16791676.6T patent/DK3349897T3/da active
- 2016-09-15 CN CN201680065865.3A patent/CN108367288A/zh active Pending
- 2016-09-15 EP EP16791676.6A patent/EP3349897B8/de not_active Not-in-force
- 2016-09-15 ES ES16791676T patent/ES2910923T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3349897B1 (de) | 2022-01-26 |
| CN108367288A (zh) | 2018-08-03 |
| EP3349897A1 (de) | 2018-07-25 |
| PL413910A1 (pl) | 2017-03-27 |
| PL237582B1 (pl) | 2021-05-04 |
| DK3349897T3 (da) | 2022-04-04 |
| EP3349897B8 (de) | 2022-02-16 |
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