ES3063801T3 - Methods of making high-throughput single-cell transcriptome libraries - Google Patents

Methods of making high-throughput single-cell transcriptome libraries

Info

Publication number
ES3063801T3
ES3063801T3 ES23187488T ES23187488T ES3063801T3 ES 3063801 T3 ES3063801 T3 ES 3063801T3 ES 23187488 T ES23187488 T ES 23187488T ES 23187488 T ES23187488 T ES 23187488T ES 3063801 T3 ES3063801 T3 ES 3063801T3
Authority
ES
Spain
Prior art keywords
methods
making high
cell transcriptome
throughput single
transcriptome libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES23187488T
Other languages
English (en)
Inventor
Jay Shendure
Junyue Cao
Frank J Steemers
Jacob Tome
Molly Gasperini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Illumina Inc
Original Assignee
University of Washington
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Illumina Inc filed Critical University of Washington
Application granted granted Critical
Publication of ES3063801T3 publication Critical patent/ES3063801T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
ES23187488T 2018-06-04 2019-06-04 Methods of making high-throughput single-cell transcriptome libraries Active ES3063801T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862680259P 2018-06-04 2018-06-04
US201962821678P 2019-03-21 2019-03-21

Publications (1)

Publication Number Publication Date
ES3063801T3 true ES3063801T3 (en) 2026-04-20

Family

ID=68769215

Family Applications (2)

Application Number Title Priority Date Filing Date
ES19814056T Active ES2966028T3 (es) 2018-06-04 2019-06-04 Bibliotecas de transcriptomas unicelulares de alto rendimiento y métodos de preparación y de uso
ES23187488T Active ES3063801T3 (en) 2018-06-04 2019-06-04 Methods of making high-throughput single-cell transcriptome libraries

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES19814056T Active ES2966028T3 (es) 2018-06-04 2019-06-04 Bibliotecas de transcriptomas unicelulares de alto rendimiento y métodos de preparación y de uso

Country Status (15)

Country Link
US (1) US20210102194A1 (es)
EP (2) EP3810774B1 (es)
JP (3) JP6986103B2 (es)
KR (2) KR102393414B1 (es)
CN (1) CN111247248A (es)
AU (3) AU2019282158B2 (es)
BR (1) BR112019027750A2 (es)
CA (2) CA3253118A1 (es)
DK (1) DK3810774T3 (es)
ES (2) ES2966028T3 (es)
FI (1) FI3810774T3 (es)
IL (2) IL314667A (es)
MX (2) MX2019015262A (es)
SG (1) SG11202000905PA (es)
WO (1) WO2019236599A2 (es)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
ES2994431T3 (en) 2017-09-22 2025-01-23 Univ Washington In situ combinatorial labeling of cellular molecules
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
US20230212656A1 (en) * 2020-02-20 2023-07-06 The Regents Of The University Of California Methods of spatially resolved single cell sequencing
EP4150106A4 (en) * 2020-05-15 2024-07-10 Integrated Dna Technologies, Inc. LIGATURE-COUPLED PCR METHODS
CN111549025B (zh) * 2020-06-08 2021-09-14 郑州大学第一附属医院 链置换引物和细胞转录组文库构建方法
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021257963A1 (en) * 2020-06-18 2021-12-23 Board Of Regents, The University Of Texas System Tiled clickseq for targeted virus whole genome sequencing
EP4185867A4 (en) * 2020-07-22 2024-08-07 Algen Biotechnologies, Inc. Methods and systems for determining drug effectiveness
EP4186060B1 (en) * 2020-07-23 2026-04-29 10X Genomics, Inc. Systems and methods for detecting and removing aggregates for calling cell-associated barcodes
CN111996598A (zh) * 2020-08-11 2020-11-27 杭州瀚因生命科技有限公司 一种单细胞染色质可及性的建库方法
EP4196600A4 (en) * 2020-08-14 2024-09-11 Factorial Diagnostics, Inc. PREPARATION OF IN SITU LIBRARIES FOR SEQUENCING
AU2021346093A1 (en) * 2020-09-15 2023-04-27 Nygen Analytics Ab Method and system for subsampling of cells from single-cell genomics dataset
US12378596B2 (en) * 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CN114606301B (zh) * 2020-12-07 2025-01-21 中国科学院脑科学与智能技术卓越创新中心 一种单细胞转录组测序的方法
CN112592968B (zh) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 高通量测序用分子标签接头及其合成方法与应用
EP4279609B1 (en) * 2020-12-31 2026-04-08 Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) Method and kit for labeling nucleic acid molecules
US12412100B2 (en) * 2021-01-22 2025-09-09 International Business Machines Corporation Cell state transition features from single cell data
CN117015617B (zh) 2021-02-23 2025-04-04 10X基因组学有限公司 基于探针的核酸和蛋白质分析
US20230048356A1 (en) * 2021-03-10 2023-02-16 Factorial Diagnostics, Inc. Cell barcoding compositions and methods
CN113160886B (zh) * 2021-04-02 2023-04-07 山东大学 基于单细胞Hi-C数据的细胞类型预测系统
CN114958996B (zh) * 2021-05-12 2022-12-20 浙江大学 一种超高通量单细胞测序试剂组合
CN113652475A (zh) * 2021-07-19 2021-11-16 中国辐射防护研究院 一种重金属铀诱导仓鼠卵巢细胞损伤的研究方法
WO2023044475A1 (en) * 2021-09-20 2023-03-23 Faeth Therapeutics, Inc. Methods of determining metabolic targets and designing nutrient modulating treatments
CN114107459B (zh) * 2021-11-11 2022-12-06 浙江大学 一种基于寡核苷酸链杂交标记的高通量单细胞测序方法
EP4453244A4 (en) * 2021-12-23 2025-12-24 Takara Bio Usa Inc METHODS AND COMPOSITIONS FOR THE PRODUCTION OF IDENTIFIABLE NUCLEIC ACID COLLECTIONS FROM CELLULAR SOURCES
EP4463567A4 (en) * 2022-01-12 2026-02-18 Jumpcode Genomics Inc METHODS AND COMPOSITIONS FOR TRANSCRIPTOME ANALYSIS
CA3244049A1 (en) * 2022-02-16 2023-08-24 Factorial Diagnostics, Inc. PREPARATION OF A LIBRARY ON SITE
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250308625A1 (en) * 2022-05-18 2025-10-02 Yale University Method for estimating a dynamic molecular program of a cell
CN114774527A (zh) * 2022-05-20 2022-07-22 良渚实验室 一种高通量单细胞转录组测序方法及其应用
CN119301275A (zh) * 2022-05-31 2025-01-10 伊路米纳有限公司 用于核酸测序的组合物和方法
EP4332234A1 (en) * 2022-08-29 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) Improved microfluidic transcriptome based screening methods
US20260103824A1 (en) * 2022-09-26 2026-04-16 The Rockefeller University Compositions and Methods for Selectively Synthesizing Triple-indexed cDNA Libraries
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
EP4594482A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
EP4627113A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Chemoenzymatic correction of false positive uracil transformations
CN115928224A (zh) * 2022-12-22 2023-04-07 广东省科学院生物与医学工程研究所 一种快速多时间生物素修饰核酸转录连缀测序文库的构建方法及其应用
CA3246669A1 (en) 2023-01-06 2024-07-11 Illumina, Inc. URACIL REDUCTION BY POLYMERASE
WO2024168092A2 (en) * 2023-02-07 2024-08-15 Parse Biosciences, Inc. Methods and kits for labeling cellular molecules for multiplex analysis
EP4720331A1 (en) 2023-05-31 2026-04-08 Illumina, Inc. False positive reduction by translesion polymerase repair
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法
WO2025072783A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025184872A1 (zh) * 2024-03-07 2025-09-12 深圳华大生命科学研究院 转录因子活性推断方法、装置、存储介质及计算机设备
WO2025240731A1 (en) * 2024-05-15 2025-11-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions to enrich target rnas for perturb-seq
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
CN120032727B (zh) * 2025-01-26 2026-02-06 河北工业大学 基于锚点选择与图学习协同优化的单细胞多组学整合方法
CN121171373A (zh) * 2025-06-18 2025-12-19 杭州联川生物技术股份有限公司 一种空间组学细胞类型注释方法、设备和介质

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
AU3539089A (en) 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
ATE144556T1 (de) 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5248607A (en) * 1988-10-27 1993-09-28 Ciba-Geigy Corporation Monoclonal antibodies and hybridomas specific for green-oat phytochrome
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH06197762A (ja) * 1992-12-28 1994-07-19 Iseki & Co Ltd タマネギのｄｎａ抽出法
WO1995021271A1 (en) 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
EP0880701A1 (en) * 1995-08-21 1998-12-02 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Methods of assessing mhc class i expression and proteins capable of modulating class i expression
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
GB2457851B (en) 2006-12-14 2011-01-05 Ion Torrent Systems Inc Methods and apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
WO2012061832A1 (en) 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
EP3305400A3 (en) 2011-10-28 2018-06-06 Illumina, Inc. Microarray fabrication system and method
EP3366348B1 (en) 2012-01-16 2023-08-23 Greatbatch Ltd. Emi filtered co-connected hermetic feedthrough, feedthrough capacitor and leadwire assembly for an active implantable medical device
CN204832037U (zh) 2012-04-03 2015-12-02 伊鲁米那股份有限公司 检测设备
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
DK3017065T3 (en) 2013-07-01 2018-11-26 Illumina Inc Catalyst-free Surface functionalization and polymer grafting
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US10017759B2 (en) * 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN104032377A (zh) * 2014-06-30 2014-09-10 北京诺禾致源生物信息科技有限公司 单细胞转录组测序文库的构建方法及其应用
WO2016066586A1 (en) 2014-10-31 2016-05-06 Illumina Cambridge Limited Novel polymers and dna copolymer coatings
US10900065B2 (en) * 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
JP6769969B2 (ja) * 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
CN107406890B (zh) * 2015-02-10 2023-07-18 亿明达股份有限公司 用于分析细胞组分的方法和组合物
WO2016154040A2 (en) * 2015-03-23 2016-09-29 The Regents Of The University Of California Biosynthetic labeling and separation of rna
EP4582556A3 (en) * 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
US12060412B2 (en) * 2016-03-21 2024-08-13 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
EP3904514A1 (en) * 2016-07-22 2021-11-03 Oregon Health & Science University Single cell whole genome libraries and combinatorial indexing methods of making thereof
JP7234114B2 (ja) * 2016-12-29 2023-03-07 イルミナ インコーポレイテッド 細胞区画内の生体分子への直交アクセスおよび細胞区画内の生体分子のタグ付けのための分析システム

Also Published As

Publication number Publication date
MX2019015262A (es) 2023-01-25
ES2966028T3 (es) 2024-04-18
EP3810774A2 (en) 2021-04-28
EP4269618A2 (en) 2023-11-01
JP2020533952A (ja) 2020-11-26
EP3810774A4 (en) 2022-06-01
AU2021261918A1 (en) 2021-12-02
BR112019027750A2 (pt) 2022-06-14
WO2019236599A2 (en) 2019-12-12
IL314667A (en) 2024-10-01
MX2023001088A (es) 2023-03-16
US20210102194A1 (en) 2021-04-08
AU2021261918B2 (en) 2025-04-03
JP6986103B2 (ja) 2021-12-22
CA3072273C (en) 2025-07-22
EP4269618B1 (en) 2025-12-24
NZ760374A (en) 2024-11-29
IL272234A (en) 2020-03-31
DK3810774T3 (da) 2023-12-11
FI3810774T3 (fi) 2023-12-11
JP2025026864A (ja) 2025-02-26
KR20220057665A (ko) 2022-05-09
RU2020102911A3 (es) 2021-08-27
SG11202000905PA (en) 2020-02-27
CA3072273A1 (en) 2019-12-12
AU2019282158A1 (en) 2020-01-16
EP4269618A3 (en) 2024-01-10
KR20200035955A (ko) 2020-04-06
KR102507415B1 (ko) 2023-03-07
RU2020102911A (ru) 2021-07-27
JP2022031810A (ja) 2022-02-22
KR102393414B1 (ko) 2022-05-02
CA3253118A1 (en) 2026-03-02
IL272234B2 (en) 2025-10-01
JP7581175B2 (ja) 2024-11-12
IL272234B1 (en) 2025-06-01
AU2019282158B2 (en) 2021-08-12
CN111247248A (zh) 2020-06-05
EP3810774B1 (en) 2023-09-13
EP4269618C0 (en) 2025-12-24
WO2019236599A3 (en) 2020-02-20
AU2025201842A1 (en) 2025-04-10

Similar Documents

Publication Publication Date Title
ES3063801T3 (en) Methods of making high-throughput single-cell transcriptome libraries
IL286643A (en) High-throughput single-cell libraries, methods for their creation and use
IL270875A (en) A method of amplifying single cell transcriptome
IL281829A (en) High throughput libraries of nuclei and single cells and methods for their preparation and use
IL263920A (en) Whole genome libraries for single cells and combinatorial indexing methods for them
LT3263715T (lt) Pavienių ląstelių transkriptomo analizės būdas
IL269288A (en) Re-synthesized recombinant nucleic acid libraries
EP4293122C0 (en) WHOLE GENOME LIBRARIES OF SINGLE CELLS FOR METHYLATION SEQUENCING
EP3784798A4 (en) ENRICHMENT OF DNA WITH TARGET SEQUENCE OF INTEREST
EP4205649C0 (en) METHOD OF MANUFACTURING AN ENZYMATIC MEMBRANE
SG11202001827UA (en) High-throughput single-cell sequencing with reduced amplification bias
EP3669018A4 (en) Binding reagent selection methods
EP3149168A4 (en) High-throughput assembly of genetic elements
IL267742A (en) Agbor kinetic exclusion of nucleic acid libraries
SG11201706636PA (en) Single-cell nucleic acids for high-throughput studies
SG11202100025QA (en) Methods for gene modification of hematopoietic cells
IL284217A (en) Automated production of viral vectors
IL257555B (en) Methods for maximum yield for obtaining microorganisms after seed treatment
GB201911958D0 (en) Methods of t cell production
EP3853379A4 (en) HIGH-THROUGH-THROUGH IDENTIFICATION OF INTERMOLECULAR INTERACTIONS
PL3601581T3 (pl) Sposoby hodowli komórkowej z użyciem inhibitorów hdac lub białek rep
GB201901576D0 (en) Production of 3C library
SI3612553T1 (sl) Izboljšan postopek za visokozmogljivostno proizvodnjo CRM-ja
EP3347471A4 (en) HIGH-EFFICIENCY SCREENING ASSAY BY CLOSED APTAMERS
IL291507A (en) Large vectors and methods for high-yield production