ES8205264A1 - Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado. - Google Patents

Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado.

Info

Publication number
ES8205264A1
ES8205264A1 ES498333A ES498333A ES8205264A1 ES 8205264 A1 ES8205264 A1 ES 8205264A1 ES 498333 A ES498333 A ES 498333A ES 498333 A ES498333 A ES 498333A ES 8205264 A1 ES8205264 A1 ES 8205264A1
Authority
ES
Spain
Prior art keywords
bacillus
desired genetic
genetic trait
recombinant
select
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES498333A
Other languages
English (en)
Other versions
ES498333A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Rochester
Original Assignee
University of Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Rochester filed Critical University of Rochester
Publication of ES498333A0 publication Critical patent/ES498333A0/es
Publication of ES8205264A1 publication Critical patent/ES8205264A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION Y SELECCION DE UN BACTERIOFAGO RECOMBINANTE QUE CONTIENE UN RASGO GENETICO DESEADO, ADECUADO PARA SER UTILIZADO EN LA TRANSFORMACION HETEROLOGA DE UN MICROOGANISMO ANFITRION DE BACILLUS. CONSITE EN OBTENER FRAGMENTOS GENETICOS QUE CODIFICAN EL RASGO GENETICO DESEADO; AISLAR FRAGMENTOS DE DNA DE UN BACTERIOFAGO; LIGAR LOS FRAGMENTOS GENETICOS CON EL DNA; INCUBAR LA MEZCLA CON DNA AISLADO DE UN SEGUNDO MICROORGANISMO DE BACILLUS QUE NO CONTIENE LOS FRAGMENTOS GENETICOS ANTES CITADOS; INCUBAR ESTA MEZCLA CON EL MICROORGANISMO ANFITRION DE BACILLUS, LISOGENICO PARA EL BACTERIOFAGO Y AUXOTROPICO PARA LA NECESIDAD DE CRECIMIENTO; SELECCIONAR EL BACILLUS ANFITRION TRANSFORMADO; DETERMINAR LA PRESENCIA DEL RASGO GENETICO DESEADO, Y OBTENER A PARTIR DEL MISMO EL BACTERIOFAGO RECOMBINANTE QUE CONTIENE EL RASGO GENETICO DESEADO POR INDUCCION DEL BACILLUS ANFITRON PROTOTROFICO.
ES498333A 1980-01-07 1981-01-05 Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado. Expired ES8205264A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11004280A 1980-01-07 1980-01-07

Publications (2)

Publication Number Publication Date
ES498333A0 ES498333A0 (es) 1982-06-01
ES8205264A1 true ES8205264A1 (es) 1982-06-01

Family

ID=22330923

Family Applications (1)

Application Number Title Priority Date Filing Date
ES498333A Expired ES8205264A1 (es) 1980-01-07 1981-01-05 Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado.

Country Status (9)

Country Link
EP (1) EP0032238B1 (es)
JP (2) JPS56113293A (es)
AT (1) ATE16943T1 (es)
DE (1) DE3071292D1 (es)
DK (1) DK3781A (es)
ES (1) ES8205264A1 (es)
IL (1) IL61837A (es)
NO (1) NO159863C (es)
ZA (1) ZA8142B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
EP0074553A3 (en) * 1981-09-11 1985-01-09 The University Of Rochester Method of increasing the yield of a product by altering a microorganism
JPS60145685A (ja) 1984-01-09 1985-08-01 Nec Corp 分布帰還型半導体レ−ザ
JPS592689A (ja) * 1982-06-04 1984-01-09 Handai Biseibutsubiyou Kenkyukai 強力な遺伝子発現能を有する新規レプリコンの作成法
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
DE3380878D1 (en) * 1982-11-01 1989-12-28 Miles Inc Method of heterologous cloning of gene in bacillus microorganism
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
IE58014B1 (en) * 1983-07-06 1993-06-16 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
EP0221923A4 (en) * 1985-03-29 1990-03-22 Bioteknika International SECRETION VECTOR.
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.

Also Published As

Publication number Publication date
ATE16943T1 (de) 1985-12-15
JPS6246153B2 (es) 1987-09-30
JPS56113293A (en) 1981-09-07
ES498333A0 (es) 1982-06-01
IL61837A0 (en) 1981-02-27
DE3071292D1 (en) 1986-01-23
NO159863B (no) 1988-11-07
JPS62163694A (ja) 1987-07-20
IL61837A (en) 1984-08-31
EP0032238A3 (en) 1982-01-20
DK3781A (da) 1981-07-08
NO803779L (no) 1981-07-08
EP0032238A2 (en) 1981-07-22
NO159863C (no) 1989-02-15
ZA8142B (en) 1982-07-28
EP0032238B1 (en) 1985-12-11

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