ES8302312A1 - Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes. - Google Patents

Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes.

Info

Publication number
ES8302312A1
ES8302312A1 ES507817A ES507817A ES8302312A1 ES 8302312 A1 ES8302312 A1 ES 8302312A1 ES 507817 A ES507817 A ES 507817A ES 507817 A ES507817 A ES 507817A ES 8302312 A1 ES8302312 A1 ES 8302312A1
Authority
ES
Spain
Prior art keywords
liquid phase
determination
component
enzyme
solid phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES507817A
Other languages
English (en)
Other versions
ES507817A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES8302312A1 publication Critical patent/ES8302312A1/es
Publication of ES507817A0 publication Critical patent/ES507817A0/es
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO INMUNOENZIMATICO EN FASE HETEROGENEA, QUE TIENE APLICACION A LA DETERMINACION DE COMPONENTES DE LA REACCION ENTRE UNA PROTEINA FIJADORA ESPECIFICAMENTE Y UNA SUSTANCIA CAPAZ DE FIJARSE CON ELLA. CONSISTE EN LA INCUBACION DE UNA DISOLUCION DE MUESTRA CON UNA CANTIDAD ESTABLECIDA DE COMPONENTE DE FIJACION EN FORMA INSOLUBLE, EN PRESENCIA DE UN REACTIVO CON UN COMPONENTE DE FIJACION MARCADO ENZIMATICAMENTE; SEGUIDA DE LA SEPARACION DE LAS FASES SOLIDA Y LIQUIDA POR CENTRIFUGACION; Y DETERMINACION DE LA ACTIVIDAD DE LA ENZIMA MARCADORA QUE HA QUEDADO EN LA FASE LIQUIDA EN UN ROTOR DE CENTRIFUGA.
ES507817A 1980-12-23 1981-12-09 Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes. Granted ES507817A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19803048884 DE3048884A1 (de) 1980-12-23 1980-12-23 Verfahren zur enzymimmunobestimmung in heterogener phase

Publications (2)

Publication Number Publication Date
ES8302312A1 true ES8302312A1 (es) 1983-01-16
ES507817A0 ES507817A0 (es) 1983-01-16

Family

ID=6120186

Family Applications (1)

Application Number Title Priority Date Filing Date
ES507817A Granted ES507817A0 (es) 1980-12-23 1981-12-09 Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes.

Country Status (9)

Country Link
US (1) US4743536A (es)
EP (1) EP0054675B1 (es)
JP (1) JPS57132060A (es)
AT (1) ATE13356T1 (es)
AU (1) AU524904B2 (es)
CA (1) CA1170180A (es)
DD (1) DD201943A5 (es)
DE (2) DE3048884A1 (es)
ES (1) ES507817A0 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60152954A (ja) * 1984-01-20 1985-08-12 Hitachi Ltd 生体試料の測定方法
JPS60152953A (ja) * 1984-01-20 1985-08-12 Hitachi Ltd 免疫測定用分析装置
JPH0799369B2 (ja) * 1985-11-25 1995-10-25 日本ケミカルリサーチ株式会社 上皮細胞増殖因子の酵素免疫測定法
JPH06105252B2 (ja) * 1986-02-05 1994-12-21 三共株式会社 遊離型サイロキシンの酵素免疫測定法
JPH0750112B2 (ja) * 1988-04-26 1995-05-31 日本電信電話株式会社 レーザ磁気免疫測定方法を実施するための検体調整方法
DE3941150A1 (de) * 1989-12-13 1991-06-20 Boehringer Mannheim Gmbh Verfahren zur bestimmung eines analyten
US5364591A (en) * 1992-06-01 1994-11-15 Eastman Kodak Company Device for moving a target-bearing solid through a liquid for detection while being contained
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5804141A (en) * 1996-10-15 1998-09-08 Chianese; David Reagent strip slide treating apparatus
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
AU2004210982B2 (en) * 2003-02-12 2008-12-18 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
US20080248511A1 (en) * 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
NL154599B (nl) * 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
AT357272B (de) * 1976-09-18 1980-06-25 Boehringer Mannheim Gmbh Verfahren und vorrichtung zum nachweis und zur bestimmung eines spezifischen bindeproteins oder einer damit bindefaehigen substanz
FR2418463A1 (fr) * 1978-02-28 1979-09-21 Nal Transfusion Sanguine Centr Procede pour le depistage ou l'identification, dans un milieu biologique, d'antigenes viraux, d'antigenes erythrocytaires ou cellulaires ou d'anticorps
US4222744A (en) * 1978-09-27 1980-09-16 Becton Dickinson & Company Assay for ligands
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays

Also Published As

Publication number Publication date
AU524904B2 (en) 1982-10-07
CA1170180A (en) 1984-07-03
ATE13356T1 (de) 1985-06-15
EP0054675B1 (de) 1985-05-15
DD201943A5 (de) 1983-08-17
EP0054675A1 (de) 1982-06-30
AU7866881A (en) 1982-07-22
JPS57132060A (en) 1982-08-16
DE3048884A1 (de) 1982-07-15
JPS6232422B2 (es) 1987-07-14
DE3170532D1 (en) 1985-06-20
ES507817A0 (es) 1983-01-16
US4743536A (en) 1988-05-10

Similar Documents

Publication Publication Date Title
ES8302312A1 (es) Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes.
EP0032782B1 (en) Method for the detection of fecal hemoglobin
EP0384130A3 (en) Myocardial infarction immunoassay
ZA856789B (en) Methods of assay
ES2006377A6 (es) Un metodo de analisis de acidos nucleicos por hibridacion.
ES8301367A1 (es) Un metodo para realizar un ensayo de ligandos en un medio poroso inerte.
ATE52140T1 (de) Bestimmungsmethode unter einbeziehung einer phasentrennung und testsatz hierfuer.
DK314987A (da) Fremgangsmaade til specifik bestemmelse af serumfructosaminindhold samt hertil egnet reagensblanding
AU4881890A (en) Assay for bone alkaline phospatase
EP0402997A3 (en) Method for determining nucleic acid
EP0399464A3 (en) Assay method for a substance with a specific sugar chain
EP0643835A4 (en) HTLV-I / HTLV-II ASSAY AND METHOD.
FI901468A0 (fi) Testipakkaus entsyymien määritykseen ja määritysmenetelmä
GB8626767D0 (en) Assays
FI68653C (fi) Metod foer bestaemning av tyroxin-bindningsindex i serum och testfoerpackning foer utfoerande av bestaemningen
ZA839522B (en) Process and reagent for determining n-carbamoylsarcosine
JPS6416964A (en) Method for immunoassay of specific saccharogenic protein
US4826771A (en) Procedure to be performed in conjunction with protein blotting or nucleic acid blotting
EP0292170A3 (en) Enzyme immunoassay
KR0151584B1 (ko) 혈액중의 특정한 결합물질 검출방법 및 상기 방법을 수행하기 위한 시험키트
JPS6146179B2 (es)
JPS54119026A (en) Simultaneous enzyme immunoassay and kit for practicing the same
GB2095400A (en) Detection of enzyme using supported enzyme binder
WO2000068415A3 (en) A method for measuring biomolecules
FR2527223B1 (fr) Anticorps anti-trehalase, reactif et procede de determination immuno-enzymatique de la trehalase