ES8305828A1 - Un procedimiento para la preparacion de una proteina de fusion. - Google Patents

Un procedimiento para la preparacion de una proteina de fusion.

Info

Publication number
ES8305828A1
ES8305828A1 ES514526A ES514526A ES8305828A1 ES 8305828 A1 ES8305828 A1 ES 8305828A1 ES 514526 A ES514526 A ES 514526A ES 514526 A ES514526 A ES 514526A ES 8305828 A1 ES8305828 A1 ES 8305828A1
Authority
ES
Spain
Prior art keywords
growth
hormone
bovine
microorganism
transformed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES514526A
Other languages
English (en)
Other versions
ES514526A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22663908&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8305828(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of ES8305828A1 publication Critical patent/ES8305828A1/es
Publication of ES514526A0 publication Critical patent/ES514526A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UNA PROTEINA DE FUSION. SE TRANSFORMA UN MICROORGANISMO CON UN VECTOR DE TRANSFERENCIA DE DNA PARA USO EN EL MANTENIMIENTO Y LA REPLICACION DE UNA SECUENCIA DEOXINUCLEOTIDICA QUE CODIFICA PARA LA PREHORMONA BOVINA DEL CRECIMIENTO. SE INCUBA EL MICROORGANISMO TRANSFORMADO PROCEDENTE DE LA ETAPA ANTERIOR EN CONDICIONES ADECUADAS PARA LA EXPRESION DE LA CITADA SECUENCIA CODIFICANTE DE LA HORMONA BOVINA DEL CRECIMIENTO. SE PURIFICA LA HORMONA BOVINA OBTENIDA DEL MEDIO DE CULTIVO DE DICHO ORGANISMO Y POR ULTIMO SE AISLA LA PROTEINA DESEADA.
ES514526A 1980-08-26 1982-07-29 Un procedimiento para la preparacion de una proteina de fusion. Granted ES514526A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18134880A 1980-08-26 1980-08-26

Publications (2)

Publication Number Publication Date
ES8305828A1 true ES8305828A1 (es) 1983-04-16
ES514526A0 ES514526A0 (es) 1983-04-16

Family

ID=22663908

Family Applications (2)

Application Number Title Priority Date Filing Date
ES504968A Expired ES8405072A1 (es) 1980-08-26 1981-08-25 Un procedimiento para la preparacion de un vector de trans- ferencia dna
ES514526A Granted ES514526A0 (es) 1980-08-26 1982-07-29 Un procedimiento para la preparacion de una proteina de fusion.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES504968A Expired ES8405072A1 (es) 1980-08-26 1981-08-25 Un procedimiento para la preparacion de un vector de trans- ferencia dna

Country Status (24)

Country Link
EP (1) EP0047600B1 (es)
JP (2) JP2534221B2 (es)
KR (1) KR830007822A (es)
AT (1) ATE72833T1 (es)
AU (1) AU544453B2 (es)
CA (1) CA1225948A (es)
CZ (1) CZ280596B6 (es)
DD (4) DD204711A5 (es)
DE (1) DE3177273D1 (es)
DK (1) DK372881A (es)
ES (2) ES8405072A1 (es)
FI (1) FI812614L (es)
GR (1) GR74350B (es)
HU (1) HU195535B (es)
IE (1) IE52755B1 (es)
IL (1) IL63628A (es)
NZ (1) NZ198050A (es)
PH (2) PH21216A (es)
PL (1) PL232797A1 (es)
PT (1) PT73569B (es)
RO (1) RO105533B1 (es)
SU (1) SU1471949A3 (es)
YU (1) YU44962B (es)
ZA (1) ZA815696B (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4443539A (en) * 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
JPS58996A (ja) * 1981-06-01 1983-01-06 ザ・ボ−ド・オブ・トラステイ−ズ・ミシガンステ−トユニバ−シテイ− 細菌中での牛成長ホルモン遺伝子のクロ−ン化
NZ201918A (en) * 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US5254463A (en) * 1981-09-18 1993-10-19 Genentech, Inc. Method for expression of bovine growth hormone
EP0278121B1 (en) * 1981-09-18 1995-09-06 Genentech, Inc. Microbially produced bovine growth hormone (BGH) and its use
GB2121054B (en) * 1982-05-25 1986-02-26 Lilly Co Eli Cloning vectors for expression of exogenous protein
EP0103395A3 (en) * 1982-08-17 1985-05-22 Biogen N.V. Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield
JPS59501852A (ja) * 1982-09-16 1984-11-08 アムジエン 鳥類の成長ホルモン
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
ZA837173B (en) * 1982-11-08 1984-05-30 Univ Case Western Reserve Genomic bovine growth hormone
AU2092983A (en) * 1982-11-08 1984-05-17 Genentech Inc. Porcine growth hormone produced by recombinant dna technology
EP0111814A3 (en) * 1982-12-16 1985-08-14 Mgi Pharma, Inc. The cloning and expression of nucleotide sequences encoding bovine growth hormone
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS6011557A (ja) * 1983-04-19 1985-01-21 ジエネツクス・コ−ポレイシヨン クロ−ンヒツジ成長ホルモン遺伝子
US4859600A (en) * 1983-04-25 1989-08-22 Genentech, Inc. Recombinant procaryotic cell containing correctly processed human growth hormone
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US5198361A (en) * 1983-07-15 1993-03-30 Bio-Technology General Corp. Plasmids for production of met-asp-gln bovine growth hormone, hosts containing the plasmids, products manufactured thereby and related methods
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US4521409A (en) * 1983-10-03 1985-06-04 Cornell Research Foundation, Inc. Use of growth hormone to enhance ruminant mammary development
US4935370A (en) * 1983-12-23 1990-06-19 Pfizer Inc. Expression plasmids for improved production of heterologous protein in bacteria
ATE66251T1 (de) * 1983-12-23 1991-08-15 Pfizer Expressionsplasmide fuer die herstellung eines heterologen proteins in bakterien.
US5489529A (en) * 1984-07-19 1996-02-06 De Boer; Herman A. DNA for expression of bovine growth hormone
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
US4861868A (en) * 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
ATE76435T1 (de) 1985-06-26 1992-06-15 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US4786501A (en) * 1985-07-15 1988-11-22 International Minerals & Chemical Corp. Cylindrical implants for the controlled release of growth hormones
US5631227A (en) * 1985-09-18 1997-05-20 The Upjohn Company Somatotropin analogs
DE3675382D1 (de) * 1985-09-18 1990-12-06 Upjohn Co Erhoehte biowirkung von saeugetier-somatotropin durch selektive deamidierung.
ES2019874B3 (es) * 1986-10-08 1991-07-16 Upjohn Co Bioactividad mejorada de somatotropina de mamiferos mediante desamidacion selectiva.
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5268284A (en) * 1988-03-11 1993-12-07 The Upjohn Company Ribosome binding site
JP2787729B2 (ja) * 1990-08-02 1998-08-20 株式会社ニッポンジーン 組換え型ミンク成長ホルモン遺伝子、組換え型ミンクプレ成長ホルモン遺伝子、ミンク成長ホルモン又はミンクプレ成長ホルモン形質転換プラスミド、及び、ミンク成長ホルモン又はミンクプレ成長ホルモン形質転換大腸菌
AU2002232758A1 (en) 2000-12-26 2002-07-08 Monsanto Technology Llc Recombinant dna vectors for expression of somatotropins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MW3378A1 (en) * 1977-11-08 1980-03-12 Genentech Inc Method and means for microbial polypeptide expression;synthetic dna and process therefor
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
IL59690A (en) * 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it

Also Published As

Publication number Publication date
PT73569A (en) 1981-09-01
KR830007822A (ko) 1983-11-07
AU544453B2 (en) 1985-05-30
FI812614A7 (fi) 1982-02-27
RO105533B1 (ro) 1995-10-01
PL232797A1 (en) 1983-07-18
ATE72833T1 (de) 1992-03-15
DD212403A5 (de) 1984-08-15
YU206281A (en) 1984-08-31
JPH09135691A (ja) 1997-05-27
ZA815696B (en) 1982-08-25
DE3177273D1 (de) 1992-04-02
ES504968A0 (es) 1984-05-16
CZ280596B6 (cs) 1996-03-13
PT73569B (en) 1982-11-09
IE52755B1 (en) 1988-02-17
DD212267A5 (de) 1984-08-08
HU195535B (en) 1988-05-30
CZ636081A3 (en) 1995-02-15
IL63628A (en) 1991-06-10
DD204711A5 (de) 1983-12-07
JP2763024B2 (ja) 1998-06-11
JPS57171999A (en) 1982-10-22
PH22847A (en) 1989-01-19
SU1471949A3 (ru) 1989-04-07
YU44962B (en) 1991-06-30
DD211581A5 (de) 1984-07-18
CA1225948A (en) 1987-08-25
IL63628A0 (en) 1981-11-30
PH21216A (en) 1987-08-21
FI812614L (fi) 1982-02-27
DK372881A (da) 1982-02-27
NZ198050A (en) 1984-12-14
IE811822L (en) 1982-02-26
GR74350B (es) 1984-06-26
JP2534221B2 (ja) 1996-09-11
AU7449681A (en) 1982-03-04
EP0047600A2 (en) 1982-03-17
ES8405072A1 (es) 1984-05-16
EP0047600B1 (en) 1992-02-26
ES514526A0 (es) 1983-04-16
EP0047600A3 (en) 1982-10-20

Similar Documents

Publication Publication Date Title
Nakahara et al. Bone and cartilage formation in diffusion chambers by subcultured cells derived from the periosteum
AR241314A1 (es) Aislado de adn, vector en base en dicho aislado cultivo de celulas tratadas con dicho vector y preparacion de factor viii humano funcional.
YU45871B (sh) Polipeptid sa humanim imunointerferonskim (ifn-gama) svojstvima
ES2056817T3 (es) Transformacion de trichoderma.
ES8600389A1 (es) Procedimiento para la produccion de una linea celular humana independiente de suero
YU206281A (en) Process for obtaining the dna transferring vector to be used in the maintaining and replication of the deoxynucleotide copying series for the bovine pre-growth hormone
YU46723B (sh) Ekspresija, prerada i sekrecija heterologog proteina pomoću kvasca
ES2144999T3 (es) Procedimiento para la preparacion de l-leucina terciaria mediante transaminacion.
FR2619314B1 (fr) Analogue du facteur viii, procede de preparation et composition pharmaceutique le contenant
ES8600783A1 (es) Procedimiento para la preparacion de la hormona polipeptidica factor de crecimiento de nervios de humano.
DE69021335D1 (de) Wachstumshormonfusionsproteine.
FI890821A0 (fi) Menetelmä kudossiirtoon soveltuvien, in vitro-viljeltyjen epiteelikalvojen säilyttämiseksi
UA6025A1 (uk) Спосіб одержання факторів 1,2,3,4,5 тейхоміціна а та їх солей 2
ES8504255A1 (es) Procedimiento para la produccion de una hormona de crecimiento de porcino.
FR2657884B1 (fr) Procede pour la preparation du facteur viii humain et d'analogues du facteur viii.
ES2099737T3 (es) Control de microsporogenesis que utilizan secuencias de promotores inducibles del exterior.
ES2084146T3 (es) Metodo para preparar lineas de celulas b humanas dependientes de factor.
DE3061854D1 (en) Process for the preparation of interferon and culture medium composition used in it
ES2059436T3 (es) Proteina de la membrana exterior f de pseudomonas aeruginosa.
ES2174848T3 (es) Cepas de levadura que expresan el gen de ldh lactica, y vectores utilizables para la obtencion de las citadas cepas.
ATE87654T1 (de) Verfahren zur herstellung von menschlichem wachstumshormon.
FI102544B1 (fi) Menetelmä E.coli-kannan valmistamiseksi
DE3683956D1 (de) In einem serumfreien medium in suspension kultivierte humane monozyten.
ES8405842A1 (es) Procedimiento de producir razas estables de celulas de mamiferos
FR2227276A1 (en) Non-toxic nitrogen-active blood serum fractions - for culture of human diploid fibro-blasts, are prepd. by fractionation using ultrafiltration membrane sieves

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20020401