ES8306796A1 - "un metodo de crear un microorganismo capaz de producir una proteina predeterminada por expresion". - Google Patents

"un metodo de crear un microorganismo capaz de producir una proteina predeterminada por expresion".

Info

Publication number
ES8306796A1
ES8306796A1 ES510011A ES510011A ES8306796A1 ES 8306796 A1 ES8306796 A1 ES 8306796A1 ES 510011 A ES510011 A ES 510011A ES 510011 A ES510011 A ES 510011A ES 8306796 A1 ES8306796 A1 ES 8306796A1
Authority
ES
Spain
Prior art keywords
heterologous gene
coli
bacillus subtilis
subtilis
gene products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES510011A
Other languages
English (en)
Other versions
ES510011A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of ES8306796A1 publication Critical patent/ES8306796A1/es
Publication of ES510011A0 publication Critical patent/ES510011A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

METODO PARA CREAR UN MICROORGANISMO CAPAZ DE PRODUCIR UNA PROTEINA PREDETERMINADA POR EXPRESION. COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE CREAN MEDIOS Y LAS CONDICIONES DE CRECIMIENTO NECESARIAS PARA DESARROLLAR UNA CEPA DE ~ABACILLUS SUBTILIS~B EN LA QUE SE HA INTRODUCIDO UN PLASMIDO CAPAZ DE REPLICARSE EN LA CEPA Y QUE TIENE UN GEN PARA LA PROTEINA PREDETERMINADA, ESTANDO DICHO GEN SITUADO Y ORIENTADO EN EL PLASMIDO DE TAL MODO QUE ESTA BAJO EL CONTROL DE UNA SECUENCIA DE OPERADOR, PROMOTOR Y SITIO DE UNION RIBOSOMICA; SEGUNDA, LA PROTEINA SEGREGADA POR LA CEPA HOSPEDANTE SE SOMETE AL CONTROL DE UN MECANISMO DE TRANSPORTE; Y POR ULTIMO, LA PROTEINA SEGREGADA ES RECUPERADA POR LOS MEDIOS DE CRECIMIENTO.
ES510011A 1980-03-10 1982-03-01 "un metodo de crear un microorganismo capaz de producir una proteina predeterminada por expresion". Granted ES510011A0 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12853780A 1980-03-10 1980-03-10
US22180080A 1980-12-31 1980-12-31

Publications (2)

Publication Number Publication Date
ES8306796A1 true ES8306796A1 (es) 1983-06-01
ES510011A0 ES510011A0 (es) 1983-06-01

Family

ID=26826682

Family Applications (2)

Application Number Title Priority Date Filing Date
ES500201A Expired ES8307895A1 (es) 1980-03-10 1981-03-09 Un metodo de producir una proteina predeterminada por circuito de bacterias gam-positivas que contienen vectores de clonacion.
ES510011A Granted ES510011A0 (es) 1980-03-10 1982-03-01 "un metodo de crear un microorganismo capaz de producir una proteina predeterminada por expresion".

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES500201A Expired ES8307895A1 (es) 1980-03-10 1981-03-09 Un metodo de producir una proteina predeterminada por circuito de bacterias gam-positivas que contienen vectores de clonacion.

Country Status (9)

Country Link
EP (1) EP0036259B1 (es)
AU (1) AU545912B2 (es)
CA (1) CA1182408A (es)
DE (1) DE3177067D1 (es)
DK (1) DK172711B1 (es)
ES (2) ES8307895A1 (es)
FI (1) FI810722L (es)
IL (1) IL62327A0 (es)
NO (1) NO810803L (es)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3005843A1 (de) * 1980-02-16 1981-09-10 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
GR75411B (es) * 1981-04-29 1984-07-16 Biogen Nv
US4508826A (en) * 1982-04-15 1985-04-02 Merck & Co., Inc. Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
US4460689A (en) * 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
FR2533583A1 (fr) * 1982-09-24 1984-03-30 Centre Nat Rech Scient Nouvel adn recombinant utile dans la preparation d'a-amylase
US4783405A (en) * 1983-01-18 1988-11-08 Eli Lilly And Company Recombinant DNA expression vectors useful in bacillus and other host cells
US4559300A (en) * 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4585739A (en) * 1983-03-07 1986-04-29 E. I. Du Pont De Nemours And Company Plasmid for foreign gene expression in B. subtilis
DK138984A (da) * 1983-03-08 1984-09-09 Rikagaku Kenkyusho Plasmid, fremgangsmaade til fremstilling heraf, mikroorganismer indeholdende samme samt fremgangsmaade til dyrkning af mikroorganismen
NZ207842A (en) 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
US4912046A (en) * 1983-06-27 1990-03-27 Genentech, Inc. Portable inducible control system
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
JPS60137291A (ja) * 1983-12-26 1985-07-20 Takeda Chem Ind Ltd 発現ベクター
DE3581528D1 (de) * 1984-07-13 1991-02-28 Chiron Corp Insulinaehnlicher wachstumsfaktor ii.
JPH062074B2 (ja) * 1984-07-27 1994-01-12 國男 山根 オリゴペプチドの製法
SE8403929L (sv) * 1984-07-31 1986-02-21 Sten Gatenbeck Rekombinant-dna-molekyl, transformerade mikroorganismer och forfarande for framstellning av penicillin v-amidas
US5032510A (en) * 1984-09-26 1991-07-16 Eli Lilly And Company Method for expression and secretion in bacillus
IT1177378B (it) * 1984-12-11 1987-08-26 Anic Spa Vettore plasmidico psm112 ad espressione in bacillus subtilis
IT1208514B (it) * 1985-03-19 1989-07-10 Eniricerche Spa Vettori plasmidici ad espressione in escherichia coli e/o bacillussubtilis.
WO1986005812A1 (en) * 1985-03-29 1986-10-09 Biotechnica International, Inc. Secretion vector
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
ATE158816T1 (de) 1990-11-26 1997-10-15 Genetics Inst Expression von pace in wirtszellen und verfahren zu dessen verwendung
GB9513371D0 (en) 1995-06-30 1995-09-06 Biocine Spa Immunogenic detoxified mutant toxins
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
CA2671261A1 (en) 1997-11-06 1999-05-20 Novartis Vaccines And Diagnostics S.R.L. Neisserial antigens
EP1047784B2 (en) 1998-01-14 2015-03-18 Novartis Vaccines and Diagnostics S.r.l. Neissera meningitidis antigens
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
EP2261338A3 (en) 1998-05-01 2012-01-04 Novartis Vaccines and Diagnostics, Inc. Neisseria meningitidis antigens and compositions
WO2000066741A2 (en) 1999-04-30 2000-11-09 Chiron S.P.A. Conserved neisserial antigens
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
JP2004508801A (ja) 1999-10-29 2004-03-25 カイロン　ソチエタ　ア　レスポンサビリタ　リミタータ ナイセリア抗原性ペプチド
PT2289545T (pt) 2000-01-17 2016-09-06 Glaxosmithkline Biologicals Sa Vacina de omv suplementada contra meningococos
CA2881568C (en) 2000-10-27 2019-09-24 Novartis Vaccines And Diagnostics, Inc. Nucleic acids and proteins from streptococcus groups a & b
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
ATE469915T1 (de) 2001-07-27 2010-06-15 Novartis Vaccines & Diagnostic Antikörper gegen das meningokokken adhäsin app
RU2331435C2 (ru) 2001-12-12 2008-08-20 Чирон Срл. Иммунизация против chlamydia trachomatis
CA2485695A1 (en) 2002-05-24 2003-12-04 Fred W. Wagner Method for universal enzymatic production of bioactive peptides
AU2003243316A1 (en) 2002-05-24 2003-12-12 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
EP1562982B1 (en) 2002-11-15 2010-05-05 Novartis Vaccines and Diagnostics S.r.l. Unexpected surface proteins in neisseria meningitidis
GB0227346D0 (en) 2002-11-22 2002-12-31 Chiron Spa 741
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
HRP20120315T1 (hr) 2003-11-21 2012-05-31 Nps Pharmaceuticals Proizvodnja peptida 2 nalik glukagonu i analoga
GB0524066D0 (en) 2005-11-25 2006-01-04 Chiron Srl 741 ii
PT2054431E (pt) 2006-06-09 2011-11-03 Novartis Ag Confórmeros de adesinas bacterianas
CN102356089B (zh) 2008-02-21 2014-02-19 诺华股份有限公司 脑膜炎球菌fHBP多肽
ITMI20090946A1 (it) 2009-05-28 2010-11-29 Novartis Ag Espressione di proteine ricombinanti
CN102596240B (zh) 2009-08-27 2015-02-04 诺华股份有限公司 包括脑膜炎球菌fHBP序列的杂交多肽
CA2779816A1 (en) 2009-10-27 2011-05-05 Novartis Ag Modified meningococcal fhbp polypeptides
FR2954349A1 (fr) 2009-12-22 2011-06-24 Agronomique Inst Nat Rech Sulfatase modifiant selectivement les glycosaminoglycanes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
FR2442271A1 (fr) * 1978-11-27 1980-06-20 Pasteur Institut Vecteur permettant l'insertion d'un gene procaryote ou eucaryote, et l'excretion de la proteine exprimee
GB2048894A (en) * 1979-05-11 1980-12-17 Gist Brocades Nv Plasmid
GR70279B (es) * 1979-09-12 1982-09-03 Univ California
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis

Also Published As

Publication number Publication date
IL62327A0 (en) 1981-05-20
AU6790281A (en) 1981-09-17
NO810803L (no) 1981-09-11
FI810722A7 (fi) 1981-09-11
ES500201A0 (es) 1983-07-01
DK172711B1 (da) 1999-06-07
CA1182408A (en) 1985-02-12
DK109581A (da) 1981-09-11
EP0036259A3 (en) 1982-06-09
EP0036259A2 (en) 1981-09-23
AU545912B2 (en) 1985-08-08
ES510011A0 (es) 1983-06-01
EP0036259B1 (en) 1989-06-14
ES8307895A1 (es) 1983-07-01
DE3177067D1 (en) 1989-07-20
FI810722L (fi) 1981-09-11

Similar Documents

Publication Publication Date Title
ES8307895A1 (es) Un metodo de producir una proteina predeterminada por circuito de bacterias gam-positivas que contienen vectores de clonacion.
Doi et al. Potential use of Bacillus subtilis for secretion and production of foreign proteins
NO911138L (no) Bakterielle vektorer.
Fahnestock et al. Expression of the staphylococcal protein A gene in Bacillus subtilis by gene fusions utilizing the promoter from a Bacillus amyloliquefaciens alpha-amylase gene
CA2120514A1 (en) Synthetic dna sequence having enhanced insecticidal activity in maize
PL291226A1 (en) Purified and isolated dna sequence encoding a polypeptide of xylanase activity and method of obtaining such sequence; dna expression structure, method of obtaining same, transformed host of microbial origin and method of obtaining same; method of xylanase activity polypeptide hyperexpression, polypeptide of xsylanase activity and application of such polypeptide; animal feed and method of obtaining same; dough for baking bread; method of degradating products containing xylanes ...
IL79291A (en) Gram-positive expression control sequences of the bacillus subtilis bacteria
DK0686195T3 (da) Fremgangsmåde og system til forhöjet produktion af kommercielt vigtige exoproteiner i grampositive bakterier
AU588793B2 (en) Method of expressing alpha-1-antitrypsin in bacteria
DE3666838D1 (en) Plasmid vectors for expression in escherichia coli and/or bacillus subtilis
AU551529B2 (en) Method and vector organism for controlled accumulation of cloned heterologous gene products in bacillus subtilis ii
DK0677110T4 (da) Rekombinant mælkesyrebakterie, der indeholder en inserteret promotor
WO1989007144A3 (en) High yield protein production system
EP0164992A3 (en) Nitrogen fixation regulator genes
ES2185972T3 (es) Sistema de expresion regulable en bacterias productoras de acido lactico.
ATE60803T1 (de) Uebertragbare induzierbare kontrollsysteme, diese enthaltende expressionsvektoren, mit diesen transformierte mikroorganismen und ihre verwendung bei der expression von exogenem protein.
LV10491A (lv) SAF polipeptids gens promoters un to pielietojums ekspresijai STREPTOMYCES sunas
AU5971090A (en) Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
Vasantha et al. Fusion of pro region of subtilisin to staphylococcal protein A and its secretion by Bacillus subtilis
MX7726E (es) Proceso biotecnologico para producir el vector plasmido de escherichia coli
TW242161B (es)
FR2582316B1 (fr) Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
EP0587763A4 (en) METHOD AND MATERIALS FOR INCLUDING DNA IN PREVOTELLA RUMINICOLA.
KR910000180A (ko) 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법
IT1231016B (it) Espressione termoinducibile di geni eterologhi in bacillus subtilis e mezzi e metodi per il suo ottenimento

Legal Events

Date Code Title Description
PC1A Transfer granted