ES8505406A1 - Procedimiento de obtener y aislar hibridomas secretores de anticuerpos, procedimiento de obtener anticuerpos y metodo de obtener polipeptidos - Google Patents
Procedimiento de obtener y aislar hibridomas secretores de anticuerpos, procedimiento de obtener anticuerpos y metodo de obtener polipeptidosInfo
- Publication number
- ES8505406A1 ES8505406A1 ES531436A ES531436A ES8505406A1 ES 8505406 A1 ES8505406 A1 ES 8505406A1 ES 531436 A ES531436 A ES 531436A ES 531436 A ES531436 A ES 531436A ES 8505406 A1 ES8505406 A1 ES 8505406A1
- Authority
- ES
- Spain
- Prior art keywords
- obtaining
- procedure
- hcmvs
- hybridomes
- isolating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/081—DNA viruses
- C07K16/085—Orthoherpesviridae (F), e.g. pseudorabies virus or Epstein-Barr virus
- C07K16/089—Cytomegalovirus
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
PROCEDIMIENTO DE PRODUCIR HIBRIDOMAS SECRETORES DE ANTICUERPOS.COMPRENDE: A) HACER REACCIONAR A LOS ANTICUERPOS NEUTRALIZANTES CON UNAS CELULAS HUMANAS EN CULTIVOS PREVIAMENTE INFECTADOS CON UN HCMV, FIJADAS CON ACETONA, Y SECADAS AL AIRE; B) EFECTUAR UNA PRIMERA SELECCION DE LOS CLONES QUE DEN LUGAR A UNA REACCION DE FIJACION, DETECTABLE POR INMUNOFLUORESCENCIA SOBRE LAS CELULAS HUMANAS Y UNA SEGUNDA SELECCION DE LOS ANTICUERPOS MONOCLONALES RETENIDAS AL FINAL DE LA PRIMERA SELECCION, QUE REACCIONAN SELECTIVAMENTE CON UNA PROTEINA VIRAL, INDUCIDA EN EL CITOPLASMA DE LAS CELULAS INFECTADAS, OBTENIDA A PARTIR DE ESTE, Y CONTENIDA EN UNA FRACCION POLIPEPTIDICA PRODUCIDA A PARTIR DEL CITOPLASMA, DESPUES DE SEPARACION PREVIA DE LAS PROTEINAS O POLIPEPTIDOS QUE TIENEN PESOS MOLECULARES DIFERENTES, Y C) RECUPERAR EL ANTICUERPO SELECCIONADO A PARTIR DE LOS PRODUCTOS DE REACCION OBTENIDOS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8305384A FR2543570B1 (fr) | 1983-03-31 | 1983-03-31 | Anticorps monoclonaux anticytomegalovirus humains, hybridomes secreteurs de ces anticorps et polypeptides porteurs d'un determinant antigenique sequentiel de cytomegalovirus humains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8505406A1 true ES8505406A1 (es) | 1985-06-01 |
| ES531436A0 ES531436A0 (es) | 1985-06-01 |
Family
ID=9287460
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES531436A Granted ES531436A0 (es) | 1983-03-31 | 1984-03-30 | Procedimiento de obtener y aislar hibridomas secretores de anticuerpos, procedimiento de obtener anticuerpos y metodo de obtener polipeptidos |
| ES540009A Granted ES540009A0 (es) | 1983-03-31 | 1985-01-31 | Procedimiento de obtencion de polipeptidos |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES540009A Granted ES540009A0 (es) | 1983-03-31 | 1985-01-31 | Procedimiento de obtencion de polipeptidos |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0122841A1 (es) |
| JP (1) | JPS59231026A (es) |
| ES (2) | ES531436A0 (es) |
| FR (1) | FR2543570B1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8404368D0 (en) * | 1984-02-20 | 1984-03-28 | Cogent Ltd | Monoclonal antibodies to human cytomegalovirus |
| US4783399A (en) * | 1984-05-04 | 1988-11-08 | Scripps Clinic And Research Foundation | Diagnostic system for the detection of cytomegalovirus |
| FR2590674B1 (fr) * | 1985-11-25 | 1989-03-03 | Inst Nat Sante Rech Med | Nouveaux reactifs de diagnostic |
| AU592142B2 (en) * | 1985-11-25 | 1990-01-04 | Cytogen Corporation | Modified viral glycoproteins, diagnostic and therapeutic uses therefore |
| WO1987003602A1 (fr) * | 1985-12-06 | 1987-06-18 | Teijin Limited | Anticorps monoclonal humain contre le virus megalocytique et procede de preparation dudit anticorps |
| DE3619902A1 (de) * | 1986-06-13 | 1988-03-03 | Biotechnolog Forschung Gmbh | Cmv-dna-strukturen, expressionsvektor dafuer, cmv-proteinstrukturen und deren verwendung |
| US5124440A (en) * | 1986-11-24 | 1992-06-23 | The Childrens Hospital, Inc. | Antibody and T cell recognition sites on glycoproteins comprising the GCI complex of human cytomegalovirus |
| US5248768A (en) * | 1986-11-24 | 1993-09-28 | The Children's Hospital, Incorporated | Immunogenic glycoproteins of human cytomegalovirus |
| US5153311A (en) * | 1986-11-24 | 1992-10-06 | The Children's Hospital, Incorporated | Immunogenic glycoproteins of human cytomegalovirus gCII |
| EP0376973A1 (en) * | 1987-08-07 | 1990-07-11 | The Children's Hospital, Incorporated | Monoclonal antibodies reactive with cytomegalovirus glycoprotein a |
| US7947274B2 (en) | 2007-01-04 | 2011-05-24 | Humabs, LLC. | Human cytomegalovirus neutralising antibodies and use thereof |
| GB0700133D0 (en) | 2007-01-04 | 2007-02-14 | Humabs Llc | Human cytomegalovirus neutralising antibodies and use thereof |
| PE20141398A1 (es) | 2008-07-16 | 2014-10-18 | Inst Research In Biomedicine | Anticuerpos neutralizantes de citomegalovirus humano |
| AU2010334809C1 (en) | 2009-12-23 | 2015-02-26 | Agenus Inc. | Binding members for human cytomegalovirus |
-
1983
- 1983-03-31 FR FR8305384A patent/FR2543570B1/fr not_active Expired
-
1984
- 1984-03-30 JP JP59063091A patent/JPS59231026A/ja active Pending
- 1984-03-30 EP EP84400648A patent/EP0122841A1/fr not_active Withdrawn
- 1984-03-30 ES ES531436A patent/ES531436A0/es active Granted
-
1985
- 1985-01-31 ES ES540009A patent/ES540009A0/es active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2543570B1 (fr) | 1985-08-09 |
| EP0122841A1 (fr) | 1984-10-24 |
| FR2543570A1 (fr) | 1984-10-05 |
| ES8601307A1 (es) | 1985-11-16 |
| ES531436A0 (es) | 1985-06-01 |
| ES540009A0 (es) | 1985-11-16 |
| JPS59231026A (ja) | 1984-12-25 |
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