ES8506090A1 - Procedimiento para preparar un plasmido de expresion per 103 - Google Patents

Procedimiento para preparar un plasmido de expresion per 103

Info

Publication number
ES8506090A1
ES8506090A1 ES533213A ES533213A ES8506090A1 ES 8506090 A1 ES8506090 A1 ES 8506090A1 ES 533213 A ES533213 A ES 533213A ES 533213 A ES533213 A ES 533213A ES 8506090 A1 ES8506090 A1 ES 8506090A1
Authority
ES
Spain
Prior art keywords
sequences
prokaryotes
preparation
gene products
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES533213A
Other languages
English (en)
Other versions
ES533213A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of ES533213A0 publication Critical patent/ES533213A0/es
Publication of ES8506090A1 publication Critical patent/ES8506090A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN PLASMIDO DE EXPRESION PER 103.COMPRENDE: A) DISOCIAR CON ENDONUCLEASA DE RESTRICCION, UN PLASMIDO (PBR 322), PARAELIMINARLE SU FRAGMENTO (ECORI-HIND III) DE 29 PARES DE BASES DE LONGITUD PROPIA DEL PLASMIDO, Y B) HACER REACCIONAR AL PLASMIDO RESULTANTE CON LIGASA, PARA INTRODUCIRLE UNA SECUENCIA DE ADN DE FORMULA (II) Y OBTENER UN PLASMIDO DE EXPRESION (PER 103) CON UNA SECUENCIA DE ADN DE FORMULA (I) Y CON MUTANTES OBTENIBLES.
ES533213A 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de expresion per 103 Expired ES8506090A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823247922 DE3247922A1 (de) 1982-12-24 1982-12-24 Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten

Publications (2)

Publication Number Publication Date
ES533213A0 ES533213A0 (es) 1985-06-16
ES8506090A1 true ES8506090A1 (es) 1985-06-16

Family

ID=6181687

Family Applications (4)

Application Number Title Priority Date Filing Date
ES528350A Granted ES528350A0 (es) 1982-12-24 1983-12-23 Procedimiento para la preparacion de interferon humano.
ES533214A Expired ES8504930A1 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de produccion
ES533213A Expired ES8506090A1 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de expresion per 103
ES533197A Granted ES533197A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES528350A Granted ES528350A0 (es) 1982-12-24 1983-12-23 Procedimiento para la preparacion de interferon humano.
ES533214A Expired ES8504930A1 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de produccion

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES533197A Granted ES533197A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido

Country Status (19)

Country Link
EP (1) EP0115613B1 (es)
JP (2) JPH088868B2 (es)
KR (1) KR930005455B1 (es)
AT (1) ATE62018T1 (es)
AU (1) AU579991B2 (es)
DD (1) DD216043A5 (es)
DE (2) DE3247922A1 (es)
DK (1) DK576983A (es)
ES (4) ES528350A0 (es)
FI (1) FI86991C (es)
GR (1) GR78766B (es)
HU (1) HU202277B (es)
IL (1) IL70523A (es)
MX (1) MX9202878A (es)
NO (1) NO173742C (es)
NZ (1) NZ206700A (es)
SU (2) SU1366064A3 (es)
UA (1) UA8035A1 (es)
ZA (1) ZA839519B (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE67786T1 (de) * 1984-08-01 1991-10-15 Boehringer Ingelheim Int Neue genetische sequenzen, die durch sie codierten interferon-peptide vom typ i und diese sie produzierende organismen.
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
IL77227A (en) * 1984-12-06 1992-08-18 Synergen Biolog Inc Recombinant methods for production of serine protease inhibitors and methods for isolation of same
FI90990C (fi) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Rekombinantti-DNA-molekyyli, transformoitu isäntäorganismi ja menetelmä interferonin valmistamiseksi
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
DE3607835A1 (de) * 1986-03-10 1987-09-24 Boehringer Ingelheim Int Hybridinterferone, deren verwendung als arzneimittel und als zwischenprodukte zur herstellung von antikoerpern und deren verwendung sowie verfahren zu ihrer herstellung
DE3642096A1 (de) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int Pferde-(gamma)-interferon
GB8813032D0 (en) * 1988-06-02 1988-07-06 Boehringer Ingelheim Int Antiviral pharmaceutical composition
WO1990005186A1 (en) * 1988-11-04 1990-05-17 The Upjohn Company Somatotropin expression using a serratia promoter
IL108584A (en) * 1994-02-07 2008-03-20 Yeda Res & Dev Cloning of interferon -alpha/beta binding protein
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
US5531880A (en) * 1994-09-13 1996-07-02 Microelectronics And Computer Technology Corporation Method for producing thin, uniform powder phosphor for display screens
FR2724665B1 (fr) * 1994-09-16 1996-12-20 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes, plasmides et cellules modifiees

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
DE3176011D1 (en) * 1980-06-12 1987-04-23 Japan Found Cancer Plasmid
ZA814375B (en) * 1980-07-01 1982-07-28 Hoffmann La Roche Interferons and process for their preparation
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0062971B1 (en) * 1981-03-27 1990-03-07 Imperial Chemical Industries Plc Genetically modified microorganisms
DE3220116A1 (de) * 1982-05-28 1983-12-01 Dr. Karl Thomae Gmbh, 7950 Biberach Mikrobiologisch hergestellte (alpha)- und ss-interferone, dna-sequenzen, die fuer diese interferone codieren, mikroorganismen, die diese genetische information enthalten, und verfahren zu ihrer herstellung
DE3247923A1 (de) * 1982-12-24 1984-06-28 Dr. Karl Thomae Gmbh, 7950 Biberach Neue oligonucleotide und verfahren zu ihrer herstellung

Also Published As

Publication number Publication date
DD216043A5 (de) 1984-11-28
ES8503372A1 (es) 1985-02-16
JPS59162888A (ja) 1984-09-13
IL70523A (en) 1991-08-16
KR930005455B1 (ko) 1993-06-22
FI86991C (fi) 1992-11-10
NO834792L (no) 1984-06-25
ZA839519B (en) 1985-08-28
FI86991B (fi) 1992-07-31
ATE62018T1 (de) 1991-04-15
EP0115613B1 (de) 1991-03-27
FI834700L (fi) 1984-06-25
DE3247922A1 (de) 1984-06-28
GR78766B (es) 1984-10-02
DK576983D0 (da) 1983-12-14
DE3382233D1 (de) 1991-05-02
NO173742C (no) 1994-01-26
UA8035A1 (uk) 1995-12-26
NZ206700A (en) 1987-11-27
EP0115613A3 (en) 1986-07-23
ES533213A0 (es) 1985-06-16
NO173742B (no) 1993-10-18
AU579991B2 (en) 1988-12-22
JPH088868B2 (ja) 1996-01-31
ES533214A0 (es) 1985-04-16
MX9202878A (es) 1992-06-30
ES533197A0 (es) 1985-02-16
JP2637328B2 (ja) 1997-08-06
SU1417800A3 (ru) 1988-08-15
KR840007105A (ko) 1984-12-05
ES8501798A1 (es) 1984-12-01
SU1366064A3 (ru) 1988-01-07
ES528350A0 (es) 1984-12-01
HU202277B (en) 1991-02-28
JPH05214000A (ja) 1993-08-24
EP0115613A2 (de) 1984-08-15
FI834700A0 (fi) 1983-12-21
IL70523A0 (en) 1984-03-30
AU2283283A (en) 1984-06-28
DK576983A (da) 1984-06-25
ES8504930A1 (es) 1985-04-16

Similar Documents

Publication Publication Date Title
ES8503372A1 (es) Procedimiento para preparar un plasmido
Taniguchi et al. Expression of the human fibroblast interferon gene in Escherichia coli.
ES8307297A1 (es) Proceso para la produccion microbiana de interferon fibro- blasto humano
HU209747B (en) Process for producing fusion proteins with eukaryotic ballastparts
EP0256424B1 (en) Homogeneous recombinant immune interferon fragments and pharmaceutical compositions containing same
DK0652903T3 (da) Nyt humant rekombinant gammainterferon
SI9011347B (sl) Plazmidi, njihova priprava in njihova uporaba pri pridobivanju plazminogenskega aktivatorja
IL115308A0 (en) Process for producing recombinant proteins plasmids and modified cells
JPS63263087A (ja) ポリペプチドの遺伝子工学的製造法
EP0072541A3 (en) Human leukocyte interferons, process for their microbial production, intermediates therefor and compositions containing them
US7642072B2 (en) Optimized interferon-beta gene
FR2556365B1 (fr) Vecteurs de clonage et d'expression de l'interferon-g, bacteries transformees et procede de preparation de l'interferon-g
HUT52552A (en) Process for production of negf
ATE5151T1 (de) Mikrobiologisch hergestelltes polypeptid mit der aminosaeuresequenz des proinsulins von affen, dna und plasmide, die fuer diese sequenz codieren, mikroorganismen, die diese genetischen informationen enthalten, und verfahren zu deren herstellung.
Luck et al. Synthesis of bovine prolactin in Escherichia coli
Seth SYNTHESIS AND USE OF DEOXYOLIGORIBONUCLEOTIDES FOR THE STUDY OF SPECIFIC INITIATION OF PROTEIN SYNTHESIS AND EXPRESSION OF EUKARYOTIC GENES IN BACTERIA.
Munson TRANSCRIPTION AND TRANSLATION INITIATION IN ESCHERICHIA COLI.
BG52178B2 (en) Method for chemical synthesis and expression of the gene of human noncystein -interferon
Zarrilli et al. In vitro expression of the promoter of the histidine operon requires the presence of distal DNA sequences.
NL8203351A (nl) Dna-transcriptie promotor; portable promotor; dna en dna-fragmenten; plasmiden en werkwijze voor het maken daarvan; getransformeerde bacterien, in het bijzonder e.coli bacterien; werkwijze voor het produceren van eiwitten.
IT1189104B (it) Vettore plasmidico ad espressione in bacillus utile per il clonaggio del gene strutturale che codifica per l'ormone della crescita umano e procedimento per la produzione di detto ormone

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000201