ES8506352A1 - Un metodo para preparar adn terminalmente unido o marcado - Google Patents
Un metodo para preparar adn terminalmente unido o marcadoInfo
- Publication number
- ES8506352A1 ES8506352A1 ES531549A ES531549A ES8506352A1 ES 8506352 A1 ES8506352 A1 ES 8506352A1 ES 531549 A ES531549 A ES 531549A ES 531549 A ES531549 A ES 531549A ES 8506352 A1 ES8506352 A1 ES 8506352A1
- Authority
- ES
- Spain
- Prior art keywords
- reagent
- terminally attached
- dna sequence
- marked dna
- transferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 102000004357 Transferases Human genes 0.000 abstract 2
- 108090000992 Transferases Proteins 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000005547 deoxyribonucleotide Substances 0.000 abstract 1
- 239000013024 dilution buffer Substances 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
METODO DE PRODUCCION DE ESTUCHES DE REACTIVOS PARA MARCAR EL EXTREMO TERMINAL 3 DE UNA SECUENCIA DE ADN SELECCIONADA.COMPRENDE LA PREPARACION DE UN ESTUCHE QUE CONTIENE UN REACTIVO CAPAZ DE EXPONER UN EXTREMO TERMIAL 3-HIDROXI DE LA SECUENCIA DE ADN, TAL COMO UNA ENZIMA, POR TRATAMIENTO CON EL MISMO DESOXIRRIBONUCLEOTIDO-TRANSFERASA TERMINAL, Y UN COMPUESTO QUE CONTIENE, AL MENOS, UN RESTO DE DESOXIRRIBONUCLEOTIDO Y, AL MENOS, UN AGENTE DE MARCAJE DETECTABLE, NO RADIACTIVO, ASOCIADO CON EL MISMO, COMPUESTO CAPAZ DE UNIRSE DE FORMA ESTABLE AL EXTREMO TERMINAL 3 EN PRESENCIA DE LA TRANSFERASA. EL REACTIVO CONTIENE ADEMAS UN TAMPON DE DILUCION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48458383A | 1983-04-13 | 1983-04-13 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8506352A1 true ES8506352A1 (es) | 1985-07-01 |
| ES531549A0 ES531549A0 (es) | 1985-07-01 |
Family
ID=23924744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES531549A Granted ES531549A0 (es) | 1983-04-13 | 1984-04-12 | Un metodo para preparar adn terminalmente unido o marcado |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0122614B1 (es) |
| JP (1) | JP2595201B2 (es) |
| AT (1) | ATE91508T1 (es) |
| AU (2) | AU2677584A (es) |
| CA (1) | CA1254525A (es) |
| DE (1) | DE3486175T2 (es) |
| DK (1) | DK185884A (es) |
| ES (1) | ES531549A0 (es) |
| IL (1) | IL71519A (es) |
| NO (1) | NO841404L (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3844485A (en) * | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| US4957858A (en) * | 1986-04-16 | 1990-09-18 | The Salk Instute For Biological Studies | Replicative RNA reporter systems |
| US4670379A (en) * | 1984-12-19 | 1987-06-02 | E. I. Du Pont De Nemours And Company | Polynucleotide hydridization assays employing catalyzed luminescence |
| CA1278755C (en) * | 1985-03-15 | 1991-01-08 | William J. Knowles | Labelling of oligonucleotides |
| WO1986005519A1 (en) * | 1985-03-15 | 1986-09-25 | James Summerton | Polynucleotide assay reagent and method |
| US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| US5047523A (en) * | 1988-08-02 | 1991-09-10 | Ortho Diagnostic Systems Inc. | Nucleic acid probe for detection of neisseria gonorrhoea |
| US5099011A (en) * | 1988-08-02 | 1992-03-24 | Ortho Diagnostic Systems Inc. | Nucleic acid probe for detection of Neisseria gonorrhoea |
| US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
| DE4001154A1 (de) * | 1990-01-17 | 1991-07-18 | Boehringer Mannheim Gmbh | Verfahren zur herstellung von modifizierten nukleinsaeuren |
| HU225970B1 (en) * | 1991-10-11 | 2008-01-28 | Acad Of Science Czech Republic | Antiviral acyclic phosphonomethoxyalkyl-substituted alkenyl purine and pyrimidine derivatives, and pharmaceutical preparations containing them |
| DE4336266A1 (de) * | 1993-10-23 | 1995-04-27 | Boehringer Mannheim Gmbh | Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren |
| US6613516B1 (en) | 1999-10-30 | 2003-09-02 | Affymetrix, Inc. | Preparation of nucleic acid samples |
| EP1700922B1 (en) | 2005-03-10 | 2016-08-24 | Roche Diagnostics GmbH | 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them |
| US7759469B2 (en) | 2005-03-10 | 2010-07-20 | Roche Diagnostics Operations, Inc. | Labeling reagent |
| EP2119797B1 (en) | 2007-01-31 | 2012-04-11 | Wako Pure Chemical Industries, Ltd. | Method of detecting amplification or deletion in genomic dna fragment |
| CN113462751A (zh) * | 2021-07-15 | 2021-10-01 | 河南赛诺特生物技术有限公司 | 一种新型探针标记方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4307189A (en) * | 1980-03-21 | 1981-12-22 | Malon Kit | Method for the quantitative determination of terminal deoxynucleotidyl transferase in biological samples |
| CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
| CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
| CA1309672C (en) * | 1983-01-27 | 1992-11-03 | Jannis G. Stavrianopoulos | Methods and structures employing non-radioactive chemically-labeled polynucleotide probes |
-
1984
- 1984-03-27 CA CA000450631A patent/CA1254525A/en not_active Expired
- 1984-04-09 NO NO841404A patent/NO841404L/no unknown
- 1984-04-10 DK DK185884A patent/DK185884A/da not_active Application Discontinuation
- 1984-04-12 JP JP59074800A patent/JP2595201B2/ja not_active Expired - Lifetime
- 1984-04-12 DE DE84104165T patent/DE3486175T2/de not_active Expired - Lifetime
- 1984-04-12 EP EP84104165A patent/EP0122614B1/en not_active Expired - Lifetime
- 1984-04-12 IL IL71519A patent/IL71519A/xx unknown
- 1984-04-12 AT AT84104165T patent/ATE91508T1/de not_active IP Right Cessation
- 1984-04-12 AU AU26775/84A patent/AU2677584A/en not_active Abandoned
- 1984-04-12 ES ES531549A patent/ES531549A0/es active Granted
-
1988
- 1988-12-20 AU AU27091/88A patent/AU2709188A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0122614B1 (en) | 1993-07-14 |
| EP0122614A2 (en) | 1984-10-24 |
| AU2677584A (en) | 1984-10-18 |
| JP2595201B2 (ja) | 1997-04-02 |
| IL71519A0 (en) | 1984-07-31 |
| ATE91508T1 (de) | 1993-07-15 |
| CA1254525A (en) | 1989-05-23 |
| DK185884A (da) | 1984-10-14 |
| NO841404L (no) | 1984-10-15 |
| DK185884D0 (da) | 1984-04-10 |
| EP0122614A3 (en) | 1987-05-06 |
| DE3486175T2 (de) | 1993-12-16 |
| ES531549A0 (es) | 1985-07-01 |
| DE3486175D1 (de) | 1993-08-19 |
| AU2709188A (en) | 1989-04-13 |
| JPS59206766A (ja) | 1984-11-22 |
| IL71519A (en) | 1988-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 20050226 |