ES8506902A1 - Un procedimiento para la purificacion de un antigeno. - Google Patents

Un procedimiento para la purificacion de un antigeno.

Info

Publication number
ES8506902A1
ES8506902A1 ES528000A ES528000A ES8506902A1 ES 8506902 A1 ES8506902 A1 ES 8506902A1 ES 528000 A ES528000 A ES 528000A ES 528000 A ES528000 A ES 528000A ES 8506902 A1 ES8506902 A1 ES 8506902A1
Authority
ES
Spain
Prior art keywords
antigen
antibody
environment
monoclonal antibodies
affinity purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES528000A
Other languages
English (en)
Other versions
ES528000A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Publication of ES528000A0 publication Critical patent/ES528000A0/es
Publication of ES8506902A1 publication Critical patent/ES8506902A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA PURIFICACION DE UN ANTIGENO.COMPRENDE: A) SELECCIONAR UN ANTICUERPO QUE TIENE UNA GRAN AFINIDAD POR EL ANTIGENO EN UN PRIMER MEDIO AMBIENTE Y UNA BAJA AFINIDAD POR EL ANTIGENO EN UN SEGUNDO MEDIO AMBIENTE, SIN QUE NINGUNO DE LOS MEDIOS AMBIENTES ALTERE DE MODO SUSTANCIALMENTE IRREVERSIBLE LAS PROPIEDADES INMUNOQUIMICAS O BIOLOGICAS DEL ANTIGENO O DEL ANTICUERPO; B) INMOVILIZAR EL ANTICUERPO SOBRE UN SOPORTE SOLIDO; C) FIJAR EL ANTIGENO AL ANTICUERPO EN EL PRIMER MEDIO AMBIENTE, CONSISTENTE EN UN LIQUIDO A UN PH DETERMINADO; D) SEPARAR LAS IMPUREZAS NO FIJADAS DEL ANTIGENO FIJADO, Y E) ELUIR EL ANTIGENO EN UNA FORMA PURIFICADA DESDE EL ANTICUERPO INMOVILIZADO, UTILIZANDO COMO ELUYENTE EL SEGUNDO MEDIO AMBIENTE, CONSISTENTE EN UNA SOLUCION QUE CONTIENE AGENTES CAOTROPICOS SELECCIONADOS ENTRE BRK, KI Y GUANIDINA Y UN PH ENTRE 4 Y 10,5.
ES528000A 1982-04-12 1983-12-13 Un procedimiento para la purificacion de un antigeno. Expired ES8506902A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36778182A 1982-04-12 1982-04-12

Publications (2)

Publication Number Publication Date
ES528000A0 ES528000A0 (es) 1985-08-01
ES8506902A1 true ES8506902A1 (es) 1985-08-01

Family

ID=23448565

Family Applications (3)

Application Number Title Priority Date Filing Date
ES521371A Expired ES8404858A1 (es) 1982-04-12 1983-04-11 Un procedimiento para la purificacion de un anticuerpo.
ES528000A Expired ES8506902A1 (es) 1982-04-12 1983-12-13 Un procedimiento para la purificacion de un antigeno.
ES528001A Expired ES8706964A1 (es) 1982-04-12 1983-12-13 Un procedimiento para fraccionar anticuerpos de suero.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES521371A Expired ES8404858A1 (es) 1982-04-12 1983-04-11 Un procedimiento para la purificacion de un anticuerpo.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES528001A Expired ES8706964A1 (es) 1982-04-12 1983-12-13 Un procedimiento para fraccionar anticuerpos de suero.

Country Status (11)

Country Link
EP (1) EP0105359A4 (es)
AT (1) AT393386B (es)
AU (1) AU622099B2 (es)
CA (1) CA1209907A (es)
CH (1) CH672028A5 (es)
DK (1) DK192191A (es)
ES (3) ES8404858A1 (es)
FI (1) FI88403C (es)
GB (1) GB2128630B (es)
IT (1) IT1219779B (es)
WO (1) WO1983003678A1 (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8328918D0 (en) * 1983-10-28 1983-11-30 Unilever Plc Alkaline phosphatase
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
US4666865A (en) * 1984-01-13 1987-05-19 Centocor, Inc. Immunoassay for biologically active human interferon-gamma employing unique monoclonal antibodies
US4789631A (en) * 1984-02-17 1988-12-06 Synbiotics Corporation Immunoassay for anti-dirofilaria immitis antibody
GB8426468D0 (en) * 1984-10-19 1984-11-28 Technology Licence Co Ltd Monoclonal antibodies
AU5259786A (en) * 1985-01-30 1986-08-07 United States Of America, The Monoclonal antibodies against chlamydial genes specific lipopolysaccharide
US4818687A (en) * 1985-02-28 1989-04-04 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
US4859611A (en) * 1985-02-28 1989-08-22 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
IL75828A (en) * 1985-07-17 1991-06-10 Univ Ramot Immobilization by biologically active proteins
NZ218336A (en) * 1985-12-09 1991-08-27 Kirin Amgen Inc Monoclonal antibodies to human pluripotent granulocyte colony stimulating factor (hpg-csf)
US5175255A (en) * 1987-03-23 1992-12-29 Amgen Inc. Methods for purification of platelet-derived growth factor
US5190859A (en) * 1987-02-26 1993-03-02 Dana-Farber Cancer Institute, Inc. Purification of LFA-3
SE468653B (sv) * 1987-03-06 1993-02-22 Perstorp Biolytica Adsorbent foer isokratisk affinitetskromatografi
JPS6463599A (en) * 1987-05-01 1989-03-09 Xoma Corp Purification of immunoglobulin by selective anti-idiotype bond
WO1989000695A1 (en) * 1987-07-14 1989-01-26 The Victoria University Of Manchester Method for the diagnosis of infections with detection of lipopolysaccharide antigens
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
US5372812A (en) * 1988-04-04 1994-12-13 The General Hospital Corporation Composition and method for acceleration of clot lysis
US5582862A (en) * 1988-04-04 1996-12-10 General Hospital Corporation Antibodies that bind to α2-antiplasmin crosslinked to fibrin which do not inhibit plasma α2-antiplasmin
CA2266339A1 (en) * 1996-09-20 1998-03-26 The General Hospital Corporation Chimeric, humanized and single chain antibodies to alpha-2-antiplasmin
EP2275443B1 (en) * 2008-04-11 2015-12-02 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly
TWI654204B (zh) 2010-11-30 2019-03-21 Chugai Seiyaku Kabushiki Kaisha 具有鈣依存性的抗原結合能力之抗體
CA2827923C (en) 2011-02-25 2021-11-23 Chugai Seiyaku Kabushiki Kaisha Fc.gamma.riib-specific fc antibody
US20150050269A1 (en) 2011-09-30 2015-02-19 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
CA3233142A1 (en) 2011-11-30 2013-06-06 Chugai Seiyaku Kabushiki Kaisha Drug containing carrier into cell for forming immune complex
WO2013125667A1 (ja) 2012-02-24 2013-08-29 中外製薬株式会社 FcγRIIBを介して抗原の消失を促進する抗原結合分子
NZ730607A (en) 2014-12-19 2022-07-01 Chugai Pharmaceutical Co Ltd Anti-myostatin antibodies, polypeptides containing variant fc regions, and methods of use
SG10201710322VA (en) 2014-12-19 2018-02-27 Chugai Pharmaceutical Co Ltd Anti-c5 antibodies and methods of use
WO2016125495A1 (en) 2015-02-05 2016-08-11 Chugai Seiyaku Kabushiki Kaisha Antibodies comprising an ion concentration dependent antigen-binding domain, fc region variants, il-8-binding antibodies, and uses therof
WO2017110981A1 (en) 2015-12-25 2017-06-29 Chugai Seiyaku Kabushiki Kaisha Anti-myostatin antibodies and methods of use
BR112019001902A2 (pt) 2016-08-05 2019-07-09 Chugai Seiyaku Kabushiki Kaisha composição para profilaxia ou tratamento de doenças relacionadas à il-8
JP7191833B2 (ja) 2017-01-30 2022-12-19 中外製薬株式会社 抗スクレロスチン抗体およびその使用
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4172124A (en) * 1978-04-28 1979-10-23 The Wistar Institute Method of producing tumor antibodies
JPS58500127A (ja) * 1981-02-26 1983-01-20 ユニリ−バ−　ナ−ムロ−ゼ　ベンノ−トシヤ−プ 回収
US4474893A (en) * 1981-07-01 1984-10-02 The University of Texas System Cancer Center Recombinant monoclonal antibodies
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies

Also Published As

Publication number Publication date
IT1219779B (it) 1990-05-24
ES521371A0 (es) 1984-05-16
GB2128630A (en) 1984-05-02
GB2128630B (en) 1986-08-28
DK192191D0 (da) 1991-11-26
GB8332644D0 (en) 1984-01-11
CA1209907A (en) 1986-08-19
ES528000A0 (es) 1985-08-01
ES8404858A1 (es) 1984-05-16
DK192191A (da) 1991-11-26
ES8706964A1 (es) 1987-07-01
AU622099B2 (en) 1992-04-02
EP0105359A4 (en) 1984-08-20
EP0105359A1 (en) 1984-04-18
AT393386B (de) 1991-10-10
ATA901983A (de) 1991-03-15
CH672028A5 (es) 1989-10-13
FI834530L (fi) 1983-12-09
FI834530A0 (fi) 1983-12-09
AU7256987A (en) 1987-09-03
ES528001A0 (es) 1987-07-01
FI88403B (fi) 1993-01-29
FI88403C (fi) 1993-05-10
WO1983003678A1 (en) 1983-10-27
IT8320550A0 (it) 1983-04-12

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970505