ES8606493A1 - Un procedimiento para la preparacion de una proteina hibrida fibrinoliticamente activa - Google Patents

Un procedimiento para la preparacion de una proteina hibrida fibrinoliticamente activa

Info

Publication number
ES8606493A1
ES8606493A1 ES538999A ES538999A ES8606493A1 ES 8606493 A1 ES8606493 A1 ES 8606493A1 ES 538999 A ES538999 A ES 538999A ES 538999 A ES538999 A ES 538999A ES 8606493 A1 ES8606493 A1 ES 8606493A1
Authority
ES
Spain
Prior art keywords
hybrid protein
chain
fibronolytically
preparation
pharmaceutical composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES538999A
Other languages
English (en)
Other versions
ES538999A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of ES538999A0 publication Critical patent/ES538999A0/es
Publication of ES8606493A1 publication Critical patent/ES8606493A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

METODO DE PREPARACION DE PROTEINAS HIBRIDAS FIBRINOLITICAMENTE ACTIVAS QUE COMPRENDE UNA CADENA DE PROTEASA DE DOS CADENAS UNIDAS A UNA CADENA DE PROTEASA DE DOS CADENAS DIFERENTES, O A LA MISMA CADENA DE LA MISMA PROTEASA. COMPRENDE LA MEZCLA DE UNA CADENA DE UNA PROTEASA CON UNA CADENA DE LA OTRA PROTEASA, OPCIONALMENTE CON DIALISIS, EN CONDICIONES OXIDATIVAS, DURANTE UNO O MAS DIAS; SEPARACION MEDIANTE TECNICAS CROMATOGRAFICAS DE LA PROTEINA HIBRIDA RESULTANTE, EN LA QUE, AL MENOS, UNA DE LAS CADENAS DERIVA DE UNA PROTEASA FIBRINOLITICAMENTE ACTIVA, DE FORMA QUE LA PROTEINA HIBRIDA TIENE UN SITIO CATALITICO ESENCIAL PARA LA ACTIVIDAD FIBRINOLITICA QUE ESTA BLOQUEADO. ESTAS PROTEINAS TIENEN APLICACIONES PARA EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
ES538999A 1983-12-24 1984-12-21 Un procedimiento para la preparacion de una proteina hibrida fibrinoliticamente activa Expired ES8606493A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB838334498A GB8334498D0 (en) 1983-12-24 1983-12-24 Compounds

Publications (2)

Publication Number Publication Date
ES538999A0 ES538999A0 (es) 1986-04-01
ES8606493A1 true ES8606493A1 (es) 1986-04-01

Family

ID=10553870

Family Applications (2)

Application Number Title Priority Date Filing Date
ES538999A Expired ES8606493A1 (es) 1983-12-24 1984-12-21 Un procedimiento para la preparacion de una proteina hibrida fibrinoliticamente activa
ES550008A Expired ES8702494A1 (es) 1983-12-24 1985-12-16 Un procedimiento para la preparacion de una proteina hibridafibrinoliticamente activa

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES550008A Expired ES8702494A1 (es) 1983-12-24 1985-12-16 Un procedimiento para la preparacion de una proteina hibridafibrinoliticamente activa

Country Status (11)

Country Link
US (3) US4752581A (es)
EP (1) EP0155387B1 (es)
JP (1) JPS60252422A (es)
AU (1) AU580023B2 (es)
DE (1) DE3479632D1 (es)
ES (2) ES8606493A1 (es)
GB (1) GB8334498D0 (es)
GR (1) GR82557B (es)
IE (1) IE57946B1 (es)
NZ (1) NZ210697A (es)
ZA (1) ZA849924B (es)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880776A (en) * 1983-12-24 1989-11-14 Beecham Group P.L.C. Plasmin A-chain urokinase B-chain hybrid protein
GB8609948D0 (en) * 1986-04-23 1986-05-29 Beecham Group Plc Compounds
GB8334498D0 (en) * 1983-12-24 1984-02-01 Beecham Group Plc Compounds
US5234686A (en) * 1985-04-03 1993-08-10 Beecham Group P.L.C. Human tissue plasminogen activator consisting essentially of t-PA residues to 160 to 527, pharmaceutical compositions and methods of treatment
US4997766A (en) * 1986-07-11 1991-03-05 American Home Products Corporation Poly-kringle plasminogen activator
US4916071A (en) * 1985-08-14 1990-04-10 American Home Products Corporation Poly-kringle plasminogen activator
US5045315A (en) * 1985-08-14 1991-09-03 American Home Products Corporation Process for treating thrombosis by administering poly-kringle plasminogen activator
ES2043610T3 (es) * 1986-01-31 1994-01-01 Sagami Chem Res Procedimiento para la produccion de un polipeptido hibrido del tipo activador de plasminogeno.
US5204255A (en) * 1986-01-31 1993-04-20 Sagami Chemical Research Center Hybrid tissue plasminogen activator/urokinase polypeptides
US4828988A (en) * 1986-05-15 1989-05-09 Smith Kline - Rit Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
US6150328A (en) * 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
US5242819A (en) * 1986-12-05 1993-09-07 Ciba-Geigy Corporation DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase
US5580559A (en) * 1986-12-05 1996-12-03 Ciba-Geigy Corporation Hybrid plasminogen activator
ZA879286B (en) * 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
NL8700013A (nl) * 1987-01-06 1988-08-01 Leuven Res & Dev Vzw Hybride plasminogeenactivatoren met verbeterde trombolytische eigenschappen en geneesmiddelen die deze plasminogeenactivatoren bevatten.
GB8705377D0 (en) * 1987-03-07 1987-04-08 Beecham Group Plc Compounds
GB8707369D0 (en) * 1987-03-27 1987-04-29 Beecham Group Plc Enzyme derivatives
NL8701021A (nl) * 1987-04-29 1988-11-16 Stichting Centraal Lab Humane t-pa(u-pa) substitutie-mutant eiwitten, daarvoor coderend recombinant dna, getransfecteerde gastheercellen, bereiding van de mutant eiwitten, en farmaceutische preparaten.
GB8711957D0 (en) * 1987-05-20 1987-06-24 Beecham Group Plc Compounds
ZA884012B (en) * 1987-06-04 1989-02-22 Zymogenetics Inc Tissue plasminogen activator analogs having modified growth factor domains
US5149533A (en) * 1987-06-04 1992-09-22 Zymogenetics, Inc. Modified t-PA with kringle-/replaced by another kringle
GB8715302D0 (en) * 1987-06-30 1987-08-05 Ordidge R J Nmr spectroscopy
AU615919B2 (en) * 1987-07-01 1991-10-17 Beecham Group Plc Hybrid plasminogen activators
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
AU2081788A (en) * 1987-07-06 1989-01-30 Genetics Institute Inc. Novel thrombolytic proteins
JP2708749B2 (ja) * 1987-08-10 1998-02-04 エーザイ株式会社 修飾型ｔＰＡ含有注射用組成物
US5023078A (en) * 1988-08-10 1991-06-11 Albert P. Halluin Plasminogen activator-heparin conjugates
US4892826A (en) * 1988-10-11 1990-01-09 Abbott Laboratories Process for the preparation of urokinase derivatives
US5198346A (en) * 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
CA2027936C (en) 1989-10-23 2001-07-24 Smithkline Beecham Corporation Cyclic anti-aggregatory peptides
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
US5227297A (en) * 1990-04-17 1993-07-13 Smithkline Beecham Corporation Affinity purification ligands
US5141862A (en) * 1990-04-17 1992-08-25 Smithkline Beecham Corporation Method of purifying tpa or plasminogen activator using a tripeptide of the formula: -X-Y-argininal wherein X and Y are selected from the group consisting of pro,phe,trp and tyr
GB9013345D0 (en) * 1990-06-14 1990-08-08 Beecham Group Plc Novel compounds
US5632986A (en) * 1991-05-09 1997-05-27 The University Of Washington Phospholipid-targeted thrombolytic agents
US20080139474A1 (en) * 1991-11-04 2008-06-12 David Israel Recombinant bone morphogenetic protein heterodimers, compositions and methods of use
WO1993009229A1 (en) * 1991-11-04 1993-05-13 Genetics Institute, Inc. Recombinant bone morphogenetic protein heterodimers, compositions and methods of use
WO1993014199A1 (en) * 1992-01-13 1993-07-22 Abbott Laboratories A pump-1 catalyzed method of making low molecular weight urokinase-type plasminogen activator
US6291206B1 (en) * 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
AU689184B2 (en) * 1993-12-07 1998-03-26 Genetics Institute, Llc BMP-12, BMP-13 and tendon-inducing compositions thereof
GB9614871D0 (en) 1996-07-15 1996-09-04 Smithkline Beecham Plc Compounds
US6727224B1 (en) * 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
DK1223990T3 (da) 1999-10-15 2004-11-29 Inst Genetics Llc Formuleringer af hyaluronsyre til tilförsel af osteogene proteiner
US6964764B2 (en) * 1999-11-13 2005-11-15 Talecris Biotherapeutics, Inc. Method of thrombolysis by local delivery of reversibly inactivated acidified plasmin
US6355243B1 (en) * 1999-11-13 2002-03-12 Bayer Corporation Method of thrombolysis by local delivery of active plasmin
US6969515B2 (en) 1999-11-13 2005-11-29 Talecris Biotherapeutics, Inc. Method of thrombolysis by local delivery of reversibly inactivated acidified plasmin
US7544500B2 (en) * 1999-11-13 2009-06-09 Talecris Biotherapeutics, Inc. Process for the production of a reversibly inactive acidified plasmin composition
US20030082233A1 (en) * 2000-12-01 2003-05-01 Lyons Karen M. Method and composition for modulating bone growth
US7226587B2 (en) * 2001-06-01 2007-06-05 Wyeth Compositions and methods for systemic administration of sequences encoding bone morphogenetic proteins
TWI267378B (en) * 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
TWI282283B (en) * 2002-05-17 2007-06-11 Wyeth Corp Injectable solid hyaluronic acid carriers for delivery of osteogenic proteins
JP5392981B2 (ja) * 2003-09-12 2014-01-22 ワイス・エルエルシー 骨形成蛋白の送達のための注入可能なリン酸カルシウム固体ロッドおよびペースト
JP4505416B2 (ja) * 2003-12-18 2010-07-21 ユストゥス−リービッヒ−ウニベルズィテート ギーセン 新規キメラプラスミノゲンアクチベータおよびその薬学的用途
WO2009149199A2 (en) * 2008-06-04 2009-12-10 Talecris Biotherapeutics, Inc. Composition, method and kit for preparing plasmin
PT2403865E (pt) 2009-03-03 2015-11-18 Grifols Therapeutics Inc Métodos de preparação de plasminogénio

Family Cites Families (11)

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US4082612A (en) * 1976-09-24 1978-04-04 Michael Reese Research Foundation Plasminogen activator complex
NZ191320A (en) * 1978-09-07 1982-09-14 Beecham Group Ltd In vivo fibrinolytic enzyme having active site blocked by hydrolytically removable group pharmaceutical compositions
AU546758B2 (en) * 1980-03-03 1985-09-19 Ryan, A.T. Feeding granular material into a gas stream
ZA831399B (en) * 1982-03-05 1984-02-29 Health Lab Service Board New fibrinolytic enzymes and methods for their production and pharmaceutical compositions containing them
EP0091240B1 (en) * 1982-04-07 1985-12-27 Beecham Group Plc Enzyme derivatives, process for preparing them and pharmaceutical compositions containing them
AU3745285A (en) * 1983-11-29 1985-06-13 Igen, Inc. Method of catalyzing chemical reactions
JPS60136520A (ja) * 1983-12-23 1985-07-20 Otsuka Pharmaceut Co Ltd フイブリン吸着性ウロキナ−ゼ複合体
GB8334498D0 (en) * 1983-12-24 1984-02-01 Beecham Group Plc Compounds
GB8334499D0 (en) * 1983-12-24 1984-02-01 Beecham Group Plc Derivatives
GB8508717D0 (en) * 1985-04-03 1985-05-09 Beecham Group Plc Composition
US4916071A (en) * 1985-08-14 1990-04-10 American Home Products Corporation Poly-kringle plasminogen activator

Also Published As

Publication number Publication date
ES550008A0 (es) 1987-01-01
US4752581A (en) 1988-06-21
EP0155387B1 (en) 1989-09-06
US4992274A (en) 1991-02-12
AU3707184A (en) 1985-07-04
AU580023B2 (en) 1988-12-22
EP0155387A3 (en) 1986-12-30
NZ210697A (en) 1989-06-28
EP0155387A2 (en) 1985-09-25
DE3479632D1 (en) 1989-10-12
ES538999A0 (es) 1986-04-01
IE843332L (en) 1985-06-24
IE57946B1 (en) 1993-05-19
GR82557B (en) 1985-04-23
ES8702494A1 (es) 1987-01-01
GB8334498D0 (en) 1984-02-01
US4908204A (en) 1990-03-13
JPS60252422A (ja) 1985-12-13
ZA849924B (en) 1986-04-30

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970612