ES8606502A1 - Un procedimiento para preparar un vector de expresion de un plasmido de levadura - Google Patents

Un procedimiento para preparar un vector de expresion de un plasmido de levadura

Info

Publication number
ES8606502A1
ES8606502A1 ES546116A ES546116A ES8606502A1 ES 8606502 A1 ES8606502 A1 ES 8606502A1 ES 546116 A ES546116 A ES 546116A ES 546116 A ES546116 A ES 546116A ES 8606502 A1 ES8606502 A1 ES 8606502A1
Authority
ES
Spain
Prior art keywords
antitrypsin
yeast
expression
human alpha
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546116A
Other languages
English (en)
Other versions
ES546116A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline RIT
Original Assignee
SmithKline RIT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline RIT filed Critical SmithKline RIT
Publication of ES546116A0 publication Critical patent/ES546116A0/es
Publication of ES8606502A1 publication Critical patent/ES8606502A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN VECTOR DE EXPRESION DE UN PLASMIDO DE LEVADURA. CONSISTE EN INTRODUCIR UN LUGAR DE ENDONUCLEASA (BAMHI) DE RESTRICCION ENTRE LAS REGIONES DE PROMOTOR Y TERMINADOR PARA LA INSERCION Y LA EXPRESION DE UNA SECUENCIA CODIFICADORA DE UN PLASMIDO. LA REGION DE PROMOTOR ES DE GEN ARG3, QUE DERIVA DE (PYEURA 3ARG3), SE TRANSPORTA POR UN FRAGMENTO (HIND III) DEL MISMO, CODIFICA ALA ORNITINA-CARBAMOIL-TRANSFERASA (OGT) Y SE AISLA EN UN FRAGMENTO (XHOI -HINC II) LA REGION DEL TERMINADOR DE ARG3 SE AISLA DE UN FRAGMENTO (HAL III -HIND III); LA SECUENCIA CODIFICADORA ES (HAAT) SE DERIVA DEL ARN MENSAJERO DEL HIGADO DE SERES HUMANOS, SE OBTIENE POR CLONACION DEL PBR322 EN EL PSTI Y TIENE TRES AMINOACIDOS BASICOS COMO EL GLUTAMICO, ACIDO ASPARFICO Y PROLINA Y EL VECTOR DE EXPRESION A OBTENER PUEDE SER (PRIT10773), (PRIT10774) Y (PRIT 10779).
ES546116A 1984-01-30 1985-08-13 Un procedimiento para preparar un vector de expresion de un plasmido de levadura Expired ES8606502A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57512284A 1984-01-30 1984-01-30

Publications (2)

Publication Number Publication Date
ES546116A0 ES546116A0 (es) 1986-04-01
ES8606502A1 true ES8606502A1 (es) 1986-04-01

Family

ID=24299041

Family Applications (3)

Application Number Title Priority Date Filing Date
ES539917A Expired ES8606402A1 (es) 1984-01-30 1985-01-29 Un procedimiento para preparar una molecula de adn recom- biante
ES546116A Expired ES8606502A1 (es) 1984-01-30 1985-08-13 Un procedimiento para preparar un vector de expresion de un plasmido de levadura
ES546115A Expired ES8703932A1 (es) 1984-01-30 1985-08-13 Un procedimiento para preparar una molecula de adn recombinante.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES539917A Expired ES8606402A1 (es) 1984-01-30 1985-01-29 Un procedimiento para preparar una molecula de adn recom- biante

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES546115A Expired ES8703932A1 (es) 1984-01-30 1985-08-13 Un procedimiento para preparar una molecula de adn recombinante.

Country Status (9)

Country Link
EP (1) EP0151102A3 (es)
JP (1) JPS60180593A (es)
AU (1) AU3819085A (es)
DK (1) DK41585A (es)
ES (3) ES8606402A1 (es)
GR (1) GR850236B (es)
IE (1) IE850208L (es)
PT (1) PT79810B (es)
ZA (1) ZA8410063B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60186290A (ja) * 1983-08-10 1985-09-21 チモ−ジエネテイツクス　インコ−ポレ−テツド アルフア−１−アンチトリプシンを細菌に表現させる方法
US4711848A (en) * 1984-03-14 1987-12-08 Zymogenetics, Inc. Site specific mutagenesis in alpha-1-antitrypsin
FR2601033B1 (fr) * 1986-07-02 1990-01-19 Transgene Sa Variants de l'alpha-antitryspine humaine glycosylee et procede pour la preparation
EP0278941A1 (en) * 1987-01-30 1988-08-17 Smithkline Biologicals S.A. Expression of the P. falciparum circumsporozoite protein by yeast
FR2864152B1 (fr) 2003-12-23 2007-11-23 Renault Sas Moteur pour vehicule comprenant au moins un piston definissant une chambre de combustion

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU577259B2 (en) * 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
BE895961A (fr) * 1983-02-21 1983-06-16 Wallone Region Procede de preparation d'un clone bacterien produisant de 1'alpha 1-antitrypsine humaine
AU3145184A (en) * 1983-08-16 1985-02-21 Zymogenetics Inc. High expression of foreign genes in schizosaccharomyces pombe
US4711848A (en) * 1984-03-14 1987-12-08 Zymogenetics, Inc. Site specific mutagenesis in alpha-1-antitrypsin
DE3585969D1 (de) * 1984-06-14 1992-06-11 Chiron Corp Protease-inhibitoren des alpha-1-antitrypsin typs mit modifizierter aktiever stelle und deren herstellung.

Also Published As

Publication number Publication date
IE850208L (en) 1985-07-30
GR850236B (es) 1985-05-29
ES546116A0 (es) 1986-04-01
DK41585A (da) 1985-07-31
PT79810B (en) 1986-10-28
ES8703932A1 (es) 1987-03-01
ES8606402A1 (es) 1986-04-16
ES539917A0 (es) 1986-04-16
ZA8410063B (en) 1985-10-30
ES546115A0 (es) 1987-03-01
EP0151102A2 (fr) 1985-08-07
AU3819085A (en) 1985-08-08
PT79810A (en) 1985-02-01
EP0151102A3 (fr) 1986-07-09
DK41585D0 (da) 1985-01-30
JPS60180593A (ja) 1985-09-14

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