ES8701842A1 - Procedimiento para la determinacion fotometrica de procalicreina en el plasma - Google Patents

Procedimiento para la determinacion fotometrica de procalicreina en el plasma

Info

Publication number
ES8701842A1
ES8701842A1 ES551764A ES551764A ES8701842A1 ES 8701842 A1 ES8701842 A1 ES 8701842A1 ES 551764 A ES551764 A ES 551764A ES 551764 A ES551764 A ES 551764A ES 8701842 A1 ES8701842 A1 ES 8701842A1
Authority
ES
Spain
Prior art keywords
plasma
extinction
time
photometric determination
prekallikrein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551764A
Other languages
English (en)
Other versions
ES551764A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES551764A0 publication Critical patent/ES551764A0/es
Publication of ES8701842A1 publication Critical patent/ES8701842A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96455—Kallikrein (3.4.21.34; 3.4.21.35)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION DE PROCALICREINA EN PLASMA POR FOTOMETRIA. EL PLASMA SE INCUBA A 30-37JC CON UN ACTIVADOR DE SUPERFICIE Y SE MIDE LA EXTINCION QUE SUMINISTRA; LUEGO SE AÑADE UN SUSTRATO CROMOGENO DE TROMBINA, EN UNA CONCENTRACION DE 0,01-2 MMOL/L Y SE MIDE, DURANTE 4 A 6 MINUTOS, A INTERVALOS CORTOS O DE FORMA CONTINUA, EL GRUPO OPTICAMENTE DETERMINABLE LIBERADO A PARTIR DE AQUEL Y SE DETERMINA LA PENDIENTE DE LA LINEA OBTENIDA AL REGISTRAR LA EXTINCION EN FUNCION DEL TIEMPO, COMO MEDIDA DEL CONTENIDO EN PROCALICREINA. COMO ACTIVADORES DE SUPERFICIE SE PUEDEN USAR ACIDO ELAGICO, SULFATIDOS Y DIOXIDO DE SILICIO FINAMENTE DIVIDIDO; EL PROCEDIMIENTO SE LLEVA A CABO EN PRESENCIA DE FOSFOLIPIDOS, ANIMALES VEGETALES O SINTETICOS Y DE IONES CALCIO (1-100 MMOL/L), LO QUE AUMENTA LA SENSIBILIDAD DEL PROCEDIMIENTO.
ES551764A 1985-02-08 1986-02-07 Procedimiento para la determinacion fotometrica de procalicreina en el plasma Expired ES8701842A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853504405 DE3504405A1 (de) 1985-02-08 1985-02-08 Bestimmung von prokallikrein

Publications (2)

Publication Number Publication Date
ES551764A0 ES551764A0 (es) 1986-12-16
ES8701842A1 true ES8701842A1 (es) 1986-12-16

Family

ID=6262060

Family Applications (1)

Application Number Title Priority Date Filing Date
ES551764A Expired ES8701842A1 (es) 1985-02-08 1986-02-07 Procedimiento para la determinacion fotometrica de procalicreina en el plasma

Country Status (9)

Country Link
US (1) US4732860A (es)
EP (1) EP0190766B1 (es)
JP (1) JPH0675518B2 (es)
AT (1) ATE43641T1 (es)
CA (1) CA1267598A (es)
DE (2) DE3504405A1 (es)
DK (1) DK164512C (es)
ES (1) ES8701842A1 (es)
NO (1) NO169022C (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5055412A (en) * 1989-03-21 1991-10-08 Proksch Gary J Factor sensitive reagent for testing of blood coagulation containing ellagic acid and divalent metal ions and method of making the same
DE69120674T2 (de) * 1990-04-17 1996-11-07 Analytical Control Syst Inc Koagulationsassays und reagenzien
US5569590A (en) * 1990-04-17 1996-10-29 Analytical Control Systems, Inc. Initial screen for abnormal platelet condition
US5637452A (en) * 1992-10-02 1997-06-10 Analytical Control Systems, Inc. Kit for an initial screen for abnormal platelet condition comprising propyl gallate or tannin and a metal ion
JP3213831B2 (ja) * 1993-08-06 2001-10-02 日本臓器製薬株式会社 被検物質の活性測定方法
JP3213832B2 (ja) * 1993-08-06 2001-10-02 日本臓器製薬株式会社 被検物質の活性測定法
JPH07109610A (ja) * 1993-08-16 1995-04-25 Tokai Kensetsu:Kk ヘルメット
ATE268477T1 (de) 1999-09-03 2004-06-15 Roche Diagnostics Corp Verfahren, reagenz und messkartusche zur bestimmung der gerinnungszeit
US6448024B1 (en) 2000-10-03 2002-09-10 Roche Diagnostics Corporation Method, reagent, cartridge, and device for determining fibrinogen
EP1367135A1 (en) * 2002-05-29 2003-12-03 Pentapharm AG Improved method for the assessment of thrombin formation in blood or plasma

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2527932C2 (de) * 1974-07-02 1983-04-21 Pentapharm AG, 4052 Basel Säureadditionssalze von Tripeptidderivaten und deren Verwendung als Substrate zur Bestimmung von Plasmakallikrein
DE2915310A1 (de) * 1979-04-14 1980-10-30 Behringwerke Ag Neues partielles thromboplastin und dessen verwendung
CA1161432A (en) * 1980-02-12 1984-01-31 Lars G. Svendsen Tripeptide derivatives and their application in assaying enzymes
JPS5848567B2 (ja) * 1980-06-04 1983-10-29 三菱化学株式会社 イソシアヌレ−ト基を含有するウレタンプレポリマ−の製造法
US4335203A (en) * 1980-07-10 1982-06-15 The Regents Of The University Of California Method for identifying potential contrast media reactors
JPS5750923A (en) * 1980-09-12 1982-03-25 Ono Pharmaceut Co Ltd Preparation of blood-plasma kallikrein and determination of blood-plasma prekallikrein
DE3244030A1 (de) * 1982-11-27 1984-05-30 Behringwerke Ag, 3550 Marburg Chromogene verbindungen, verfahren zu ihrer herstellung und ihre verwendung
DE3311287A1 (de) * 1983-03-28 1984-10-04 Behringwerke Ag, 3550 Marburg Verfahren zur fotometrischen bestimmung der aktivierten partiellen thromboplastinzeit und reagenz dazu

Also Published As

Publication number Publication date
US4732860A (en) 1988-03-22
DK164512C (da) 1992-11-23
EP0190766B1 (de) 1989-05-31
ES551764A0 (es) 1986-12-16
DE3504405A1 (de) 1986-08-14
DK164512B (da) 1992-07-06
CA1267598A (en) 1990-04-10
DK62186D0 (da) 1986-02-07
ATE43641T1 (de) 1989-06-15
NO860200L (no) 1986-08-11
JPS61185199A (ja) 1986-08-18
JPH0675518B2 (ja) 1994-09-28
NO169022C (no) 1992-04-29
DK62186A (da) 1986-08-09
NO169022B (no) 1992-01-20
EP0190766A3 (en) 1986-12-10
EP0190766A2 (de) 1986-08-13
DE3663684D1 (de) 1989-07-06

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970519