ES8900255A1 - Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica - Google Patents

Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica

Info

Publication number
ES8900255A1
ES8900255A1 ES548941A ES548941A ES8900255A1 ES 8900255 A1 ES8900255 A1 ES 8900255A1 ES 548941 A ES548941 A ES 548941A ES 548941 A ES548941 A ES 548941A ES 8900255 A1 ES8900255 A1 ES 8900255A1
Authority
ES
Spain
Prior art keywords
vectors
lipolytica
dna
transformation
yarrowia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES548941A
Other languages
English (en)
Other versions
ES548941A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Publication of ES8900255A1 publication Critical patent/ES8900255A1/es
Publication of ES548941A0 publication Critical patent/ES548941A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Magnetic Ceramics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Electrotherapy Devices (AREA)
  • Protection Of Transformers (AREA)
  • Treatment Of Sludge (AREA)
  • Saccharide Compounds (AREA)
  • Air-Conditioning For Vehicles (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Paper (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)

Abstract

SE REFIERE LA PRESENTE INVENCION A LA TRANSFORMACION DE YARROWIA LIPOLYTICA, A VECTORES UTILES PARA ELLO QUE CONTENGAN DNA MICROBIANO Y DNA CROMASOMICO DE Y. LIPOLYTICA, A TRANSFORMANTES DE E. COLI Y Y. LIPOLYTICA, ASI COMO A VECTORES LANZADERA INTEGRANTES PARA EL CLONAJE TRANSGENERICO ESCHERICHIA-YARROWIA. LOS VECTORES O SUBCLONES PERMITEN LA CREACION DE VECTORES DE CLONAJE DE Y. LYPOLYTICA EN LA QUE PUEDEN INSERTARSE SEGMENTOS ESPECIFICOS O ALEATORIOS DE DNA, PUDIENDOSE UTILIZAR LOS VECTORES RESULTANTES PARA TRANSFORMAR UN MICROBIO HUESPED, ESPECIALMENTE Y. LIPOLYTIA, Y MEJORAR ASI SUS CARACTERISTICAS DE FERMENTACION Y CON ELLO SU UTILIZACION INDUSTRIAL.
ES548941A 1983-10-06 1985-11-15 Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica Expired ES8900255A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US53959183A 1983-10-06 1983-10-06

Publications (2)

Publication Number Publication Date
ES8900255A1 true ES8900255A1 (es) 1989-05-01
ES548941A0 ES548941A0 (es) 1989-05-01

Family

ID=24151875

Family Applications (3)

Application Number Title Priority Date Filing Date
ES536546A Expired ES8605038A1 (es) 1983-10-06 1984-10-05 Un procedimiento para la transformacion de yarrowia lipolytica
ES548941A Expired ES8900255A1 (es) 1983-10-06 1985-11-15 Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica
ES548940A Expired ES8802399A1 (es) 1983-10-06 1985-11-15 Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES536546A Expired ES8605038A1 (es) 1983-10-06 1984-10-05 Un procedimiento para la transformacion de yarrowia lipolytica

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES548940A Expired ES8802399A1 (es) 1983-10-06 1985-11-15 Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli.

Country Status (17)

Country Link
EP (1) EP0138508B1 (es)
JP (2) JP2559689B2 (es)
KR (1) KR870000239B1 (es)
AT (1) ATE73848T1 (es)
AU (1) AU554118B2 (es)
BR (1) BR8404984A (es)
DE (1) DE3485589D1 (es)
DK (2) DK171878B1 (es)
ES (3) ES8605038A1 (es)
FI (1) FI85987C (es)
IE (1) IE57685B1 (es)
IL (1) IL73119A (es)
IN (1) IN163393B (es)
NO (1) NO173743C (es)
NZ (1) NZ209794A (es)
PH (1) PH25363A (es)
ZA (1) ZA847824B (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2579617B2 (fr) * 1985-04-02 1987-06-26 Agronomique Inst Nat Rech Vecteurs de transformation de la levure yarrowia lipolytica, procede de transformation et levure transformee
EP0166659B1 (fr) * 1984-06-26 1989-08-30 Institut National De La Recherche Agronomique (Inra) Vecteurs de transformation de la levure yarrowia lipolytica, procédé de transformation et levure transformée
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
GB8529275D0 (en) * 1985-11-28 1986-01-02 Whitbread & Co Plc Dna recombination
RU2198923C2 (ru) * 1986-03-18 2003-02-20 Пфайзер Инк. Нуклеотидная последовательность гена leu2 yarrowia lipolytica (варианты), рекомбинантный днк-материал (варианты), штамм дрожжей yarrowia lipolytica (варианты)
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
FR2734843A1 (fr) * 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
GB9715905D0 (en) * 1997-07-28 1997-10-01 Ciba Geigy Ag Plant phosphoribosyl transferase and DNA coding thereof
FR2782733B1 (fr) * 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
ATE496134T1 (de) * 2002-03-29 2011-02-15 Xoma Technology Ltd Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
KR20090058077A (ko) * 2007-12-04 2009-06-09 충북대학교 산학협력단 생물체 정보의 염색체 내 표지 방법 및 그 정보가 표지된생물체
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
KR20170010072A (ko) * 2008-05-23 2017-01-25 누셀리스 인크. 효모를 사용한 스쿠알렌 생산
WO2010135624A2 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
EP0138508B1 (en) 1992-03-18
DK171879B1 (da) 1997-07-28
ATE73848T1 (de) 1992-04-15
NO173743C (no) 1994-01-26
IE842535L (en) 1985-04-06
DK148195A (da) 1995-12-28
NO844001L (no) 1985-04-09
ES8802399A1 (es) 1988-05-16
NZ209794A (en) 1989-01-27
IE57685B1 (en) 1993-02-24
PH25363A (en) 1991-05-13
DE3485589D1 (de) 1992-04-23
JPS60199386A (ja) 1985-10-08
AU554118B2 (en) 1986-08-07
DK477084A (da) 1985-05-15
DK477084D0 (da) 1984-10-05
KR850003167A (ko) 1985-06-13
FI843932L (fi) 1985-04-07
ES548941A0 (es) 1989-05-01
JP2559689B2 (ja) 1996-12-04
KR870000239B1 (ko) 1987-02-18
DK171878B1 (da) 1997-07-28
NO173743B (no) 1993-10-18
FI85987C (fi) 1992-06-25
BR8404984A (pt) 1985-08-20
ES536546A0 (es) 1986-02-16
JP2718659B2 (ja) 1998-02-25
AU3387684A (en) 1985-04-18
IL73119A (en) 1990-01-18
ES8605038A1 (es) 1986-02-16
JPH09131176A (ja) 1997-05-20
FI843932A0 (fi) 1984-10-05
EP0138508A1 (en) 1985-04-24
IL73119A0 (en) 1984-12-31
ZA847824B (en) 1986-05-28
IN163393B (es) 1988-09-17
ES548940A0 (es) 1988-05-16
FI85987B (fi) 1992-03-13

Similar Documents

Publication Publication Date Title
MX169933B (es) Procedimiento para la produccion biologica de amidas
MX2021002157A (es) Composiciones de tratamiento que comprenden un sistema surfactante y una oligoamina.
ES2058541T3 (es) Un retardante de la llama y una composicion de resina retardante de la llama.
GT198065892A (es) Derivados de acidos 6 b-hidroxialquilpenicilanicos como inhibidores de b-lactamasa.
ES2175244T3 (es) Empleo de acido poliasparaginico y de sus sales.
ES2072560T3 (es) Procedimiento de decapado de materiales de acero, especialmente de acero inoxidable.
ES2126093T3 (es) Composiciones liquidas estables y su uso.
ES2064610T3 (es) Mezcla de arcilla que tiene resistencia a la contaminacion.
CO4290320A1 (es) Composicion para inhibir la corrosion de metales utilizando acidos politartaricos
SE0100714D0 (sv) Reaction vessel and method for distributing fluid in such a vessel
GB9017611D0 (en) Method of decolourising water
GB1066189A (en) Improvements in piston and piston rod connections and methods of constructing such connections
ES2064434T3 (es) Procedimiento para la conservacion de sistemas acuosos que pueden sufrir degradacion microbiologica.
SE7604641L (sv) Beleggningskomposition
GB1034109A (en) Improvements in or relating to valves
ES8600775A1 (es) Procedimiento para curtir posteriormente cuero curtido con marterial mineral
JPS51111913A (en) An injection method of the liquid-gas
JPS5216374A (en) Method for converting waste white clay to feed
GB549111A (en) An improved method of softening water
BE609874A (fr) Perfectionnements apportés aux installations de télécommande de vannes, notamment de vannes sur les navires tels que les navires pétroliers
UA13176A (uk) Спосіб очищеhhя стічhих вод
JPS5386080A (en) Cultivation of microorganisms
DK438883A (da) 1,2,3,4-tetrahydro-1-aminomethyl-4-phenyl-isoquinoliner, samt fremgangsmaade til fremstilling heraf
AR248382A1 (es) Metodo para inhibir el crecimiento de por lo menos un microorganismo en un sistema industrial susceptible al crecimiento de un microorganismo.
GB1119345A (en) Treated slag and process for the production thereof

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20100623