ATE496134T1 - Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden - Google Patents

Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden

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Publication number
ATE496134T1
ATE496134T1 AT03808015T AT03808015T ATE496134T1 AT E496134 T1 ATE496134 T1 AT E496134T1 AT 03808015 T AT03808015 T AT 03808015T AT 03808015 T AT03808015 T AT 03808015T AT E496134 T1 ATE496134 T1 AT E496134T1
Authority
AT
Austria
Prior art keywords
methods
expression
increased expression
recombinant polypeptides
materials
Prior art date
Application number
AT03808015T
Other languages
English (en)
Inventor
Arnold Horwitz
Original Assignee
Xoma Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xoma Technology Ltd filed Critical Xoma Technology Ltd
Application granted granted Critical
Publication of ATE496134T1 publication Critical patent/ATE496134T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT03808015T 2002-03-29 2003-03-31 Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden ATE496134T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36853002P 2002-03-29 2002-03-29
PCT/US2003/010154 WO2004033693A1 (en) 2002-03-29 2003-03-31 Methods vectors plasmids and methods for increasing expression of recombinant polypeptides

Publications (1)

Publication Number Publication Date
ATE496134T1 true ATE496134T1 (de) 2011-02-15

Family

ID=32093683

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03808015T ATE496134T1 (de) 2002-03-29 2003-03-31 Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden

Country Status (9)

Country Link
US (4) US7192737B2 (de)
EP (2) EP2311962B1 (de)
JP (2) JP4554370B2 (de)
AT (1) ATE496134T1 (de)
AU (2) AU2003300588B2 (de)
CA (1) CA2492008C (de)
DE (1) DE60335794D1 (de)
DK (1) DK1492874T3 (de)
WO (1) WO2004033693A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100311954A1 (en) * 2002-03-01 2010-12-09 Xencor, Inc. Optimized Proteins that Target Ep-CAM
ATE496134T1 (de) * 2002-03-29 2011-02-15 Xoma Technology Ltd Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden
US20050009097A1 (en) * 2003-03-31 2005-01-13 Better Marc D. Human engineered antibodies to Ep-CAM
CA2531698A1 (en) * 2003-07-09 2005-01-27 Sentigen Biosciences, Inc. Method for assaying protein-protein interaction
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
US20050202539A1 (en) * 2004-03-11 2005-09-15 Hydra Biosciences System for screening eukaryotic membrane proteins
JP2008522589A (ja) * 2004-12-03 2008-07-03 ゾーマ テクノロジー リミテッド 組換えタンパク質の発現のための方法及び材料
US20060154336A1 (en) * 2004-12-14 2006-07-13 Millennium Pharmaceuticals, Inc. Nucleic acid molecules for optimized CAB-2 expression and their use
US20070072174A1 (en) * 2005-09-28 2007-03-29 Sayler Gary S Bioreporter for detection of microbes
EP1783228A1 (de) * 2005-11-08 2007-05-09 ETH Zürich Rekombinante Expression von Multiproteinkomplexen mit Hilfe von Multigenen
US8278421B2 (en) 2006-03-20 2012-10-02 Xoma Techolology Ltd. Human antibodies specific for gastrin materials and methods
HRP20140081T1 (hr) 2006-08-18 2014-03-28 Novartis Ag Prlr-specifiäśna antitijela i njihova uporaba
CA2672120A1 (en) 2006-12-07 2008-06-12 Novartis Ag Antagonistic antibodies against ephb3
US7935808B2 (en) 2007-03-30 2011-05-03 Abbott Laboratories Recombinant expression vector elements (rEVEs) for enhancing expression of recombinant proteins in host cells
EP2189529B1 (de) * 2007-08-10 2017-01-11 Toto Ltd. Verfahren zur herstellung rekombinanter säugerzellen und verfahren zur herstellung eines zielproteins
WO2009055343A2 (en) 2007-10-22 2009-04-30 Schering Corporation Fully human anti-vegf antibodies and methods of using
US8318162B2 (en) * 2009-07-16 2012-11-27 Xoma Technology Ltd. Antibodies to high molecular weight melanoma associated antigen
SG177756A1 (en) * 2009-07-29 2012-02-28 Tecnogen Spa Improved human long pentraxin 3 expression system and uses thereof
WO2012011955A2 (en) * 2010-07-21 2012-01-26 Duke University Compositions and methods for production and screening of monoclonal antibodies
WO2012077128A1 (en) * 2010-12-07 2012-06-14 Intas Biopharmaceuticals Limited A novel cell line development process to produce recombinant proteins using twin vector expression system
ES2811648T3 (es) * 2013-03-12 2021-03-12 Agc Biologics Inc Expresión de proteína biotecnológica mejorada que usa un activador CHEF1 híbrido
US9388239B2 (en) 2014-05-01 2016-07-12 Consejo Nacional De Investigation Cientifica Anti-human VEGF antibodies with unusually strong binding affinity to human VEGF-A and cross reactivity to human VEGF-B
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
EP3510047A1 (de) 2016-09-07 2019-07-17 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Anti-nkp46-antikörper und therapeutische verwendung davon
US10318741B2 (en) 2016-11-21 2019-06-11 Bank Of America Corporation Data security and risk ranking tool
IL253984A0 (en) 2017-08-14 2017-09-28 Yeda Res & Dev Immunotherapy against aranviruses that bind through transferrin receptor 1 (tfr1
JP7278261B2 (ja) 2017-08-23 2023-05-19 ノバルティス アーゲー マルチコピー遺伝子タンパク質発現系
CN117343193A (zh) * 2017-11-02 2024-01-05 西雅图免疫公司 双特异性抗体及其制备和使用方法
IL276627A (en) 2020-08-10 2022-03-01 Yeda Res & Dev Compositions for diagnosis and treatment of coronavirus infections
CN117701410A (zh) * 2023-12-18 2024-03-15 中国海洋大学 介导协氧蛋白和褐藻胶裂解酶的双转录单元重组菌和应用

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4612282A (en) 1981-12-15 1986-09-16 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Monoclonal antibodies reactive with human breast cancer
IN163393B (de) * 1983-10-06 1988-09-17 Pfizer
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US5198541A (en) 1987-08-11 1993-03-30 New York University Dna encoding bactericidal/permeability-increasing proteins
US5576184A (en) 1988-09-06 1996-11-19 Xoma Corporation Production of chimeric mouse-human antibodies with specificity to human tumor antigens
GB9115364D0 (en) 1991-07-16 1991-08-28 Wellcome Found Antibody
EP1291360A1 (de) 1991-12-13 2003-03-12 Xoma Corporation Verfahren und Materialien zur Herstellung von modifizierten Antikörper Variablen Domänen und deren therapeutischen Verwendung
WO1993023434A2 (en) 1992-05-19 1993-11-25 Xoma Corporation Bpi-immunoglobulin fusion proteins
ATE178652T1 (de) 1992-05-19 1999-04-15 Xoma Corp Verbessertes verfahren zur herstellung von endotoxinbindungsproteinen
US5420019A (en) 1993-02-02 1995-05-30 Xoma Corporation Stable bactericidal/permeability-increasing protein muteins
US5348942A (en) 1993-03-12 1994-09-20 Xoma Corporation Therapeutic uses of bactericidal/permeability increasing protein products
US5652332A (en) 1993-03-12 1997-07-29 Xoma Biologically active peptides from functional domains of bactericidal/permeability-increasing protein and uses thereof
US5858974A (en) 1995-07-20 1999-01-12 Xoma Corporation Anti-fungal peptides
US5733872A (en) 1993-03-12 1998-03-31 Xoma Corporation Biologically active peptides from functional domains of bactericidal/permeability-increasing protein and uses thereof
AU694108B2 (en) 1993-03-12 1998-07-16 Xoma Corporation Biologically active peptides from functional domains of bactericidal/permeability-increasing protein and uses thereof
MX9401807A (es) 1993-03-12 1995-01-31 Xoma Corp Usos terapeuticos de productos de proteina que incrementan lapermeabilidad/bactericidas.
US5447913A (en) 1994-03-11 1995-09-05 Xoma Corporation Therapeutic uses of bactericidal/permeability-increasing protein dimer products
CA2200069C (en) 1994-09-15 2000-09-26 Xoma Corporation Anti-fungal peptides
US5866402A (en) 1995-05-05 1999-02-02 Chiron Corporation Chimeric MCP and DAF proteins with cell surface localizing domain
US5851802A (en) 1996-03-22 1998-12-22 Xoma Corporation Methods for recombinant microbial production of fusion proteins and BPI-derived peptides
US5998174A (en) * 1997-05-12 1999-12-07 University Of Pittsburgh Of The Commonwealth System Of Higher Education Multigene vectors
US6013631A (en) 1998-06-19 2000-01-11 Xoma Corporation Bactericidal/permeability-increasing protein (BPI) deletion analogs
CA2389633A1 (en) 1999-11-01 2001-05-10 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
ATE496134T1 (de) * 2002-03-29 2011-02-15 Xoma Technology Ltd Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden
US9320293B2 (en) 2008-06-06 2016-04-26 Gold Medal Products Company Popcorn kettle
US9402401B1 (en) 2008-11-03 2016-08-02 William P. Benzel Extruded three loop pretzel having a twist-knot appearance at its middle portion, apparatus and die for making said pretzel, and method of forming said pretzel
US8489677B2 (en) 2009-10-28 2013-07-16 Advanced Businesslink Corporation Session pooling for legacy application tasks
KR101858484B1 (ko) 2010-12-28 2018-06-27 히타치 긴조쿠 가부시키가이샤 페라이트 소결 자석 및 그 제조 방법
US9603845B2 (en) 2012-05-15 2017-03-28 Taiho Pharmaceutical Co., Ltd. Prophylactic agent and/or therapeutic agent for stress urinary incontinence
US9410427B2 (en) 2012-06-05 2016-08-09 United Technologies Corporation Compressor power and torque transmitting hub
PT2882444T (pt) 2012-10-22 2025-11-21 Zs Pharma Inc Silicato de zircónio microporoso para tratamento de hipercalemia
JP6453542B2 (ja) 2013-02-14 2019-01-16 ソウル セミコンダクター カンパニー リミテッド 半導体装置及びこれの製造方法
KR20150087669A (ko) 2014-01-22 2015-07-30 삼성전기주식회사 컨커런트 다중밴드 고주파 증폭회로
US9503125B2 (en) 2014-05-08 2016-11-22 Sandisk Technologies Llc Modified trellis-based min-max decoder for non-binary low-density parity-check error-correcting codes
US9402465B1 (en) 2014-06-11 2016-08-02 Paul Edward LaForge Shaving brush with vented holder
US9218176B1 (en) 2014-06-13 2015-12-22 International Business Machines Corporation Software deployment in a distributed virtual machine environment

Also Published As

Publication number Publication date
JP4554370B2 (ja) 2010-09-29
US20030203447A1 (en) 2003-10-30
DE60335794D1 (de) 2011-03-03
US8497096B2 (en) 2013-07-30
US20120178126A1 (en) 2012-07-12
EP2311962B1 (de) 2013-03-13
EP2311962A1 (de) 2011-04-20
JP2005528925A (ja) 2005-09-29
AU2009200829A1 (en) 2009-03-19
DK1492874T3 (da) 2011-03-14
EP1492874A1 (de) 2005-01-05
JP2006141415A (ja) 2006-06-08
EP1492874B1 (de) 2011-01-19
US7192737B2 (en) 2007-03-20
AU2003300588B2 (en) 2008-12-04
US20080145895A1 (en) 2008-06-19
CA2492008C (en) 2012-06-26
CA2492008A1 (en) 2004-04-22
WO2004033693A1 (en) 2004-04-22
AU2003300588A1 (en) 2004-05-04
US7993915B2 (en) 2011-08-09
US20070281334A1 (en) 2007-12-06

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