FI104255B - Förfarande för framsätllning av fusionsproteiner innehållande N-terminalfragmenter av mänsklig serumalbumin - Google Patents

Förfarande för framsätllning av fusionsproteiner innehållande N-terminalfragmenter av mänsklig serumalbumin Download PDF

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Publication number
FI104255B
FI104255B FI915073A FI915073A FI104255B FI 104255 B FI104255 B FI 104255B FI 915073 A FI915073 A FI 915073A FI 915073 A FI915073 A FI 915073A FI 104255 B FI104255 B FI 104255B
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Finland
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hsa
variant
sequence
polypeptide
terminal
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FI915073A
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English (en)
Finnish (fi)
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FI915073A0 (fi
FI104255B1 (sv
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David James Ballance
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Delta Biotechnology Ltd
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Publication of FI104255B1 publication Critical patent/FI104255B1/sv
Publication of FI104255B publication Critical patent/FI104255B/sv

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/31—Fusion polypeptide fusions, other than Fc, for prolonged plasma life, e.g. albumin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (5)

1. Ett förfarande för framställning av en fusionspolypeptid omfattande, som ät-minstone en del av dess N-terminala del, en N-terminal del av HSA eller en variant därav och, som ätminstone en del av dess C-terminala del, en annan polypeptid med 5 undantag för att när nämnda N-terminala del av HSA är 1-n-delen, där n är 369-419 eller en en variant därav, sä är nämnda polypeptid (a) 585-1578-delen av humanfib-ronektin eller variant därav, (b) 1-368-delen av CD4 eller en variant därav, (c) plätt-härstammande tillväxtfaktor eller en variant därav, (d) transformerande tillväxtfaktor β eller en variant därav, (e) 1-261-delen av fullt utvecklat humanplasmafibronektin 10 eller en variant därav, (f) 278-578-delen av fullt utvecklat humanplasma-fibronektin eller en variant därav, (g) 1-272-delen av fullt utvecklat human-von Willebrandts faktor eller en variant därav eller (h) alfa-1-antitrypsin eller en variant därav, känne-tecknat av att i en transformerad värdcell uttrycks en polynukleotidsekvens, som kodar nämnda fusionspolypeptid.
2. Förfarande enligt patentkrav 1, kännetecknat av att fusionspolypeptiden des-sutom omfattar ätminstone en N-terminal aminosyra sträckande sig utöver den del som motsvarar den N-terminala delen av HSA. 20
3. Förfarande enligt patentkrav 1 eller 2, kännetecknat av att det finns en spalt- bar region vid kopplingspunkten för nämnda N-terminala eller C-terminala delar av fusionspolypeptid. • I
: 4. Förfarande enligt nägot av föregäende patentkrav, kännetecknat av att nämn- 25 da C-terminala del av fusionspolypeptid är 585-1578-delen av humanplasmafibro- : ’ * *i nektin eller varianter därav. ··· • · • · « • · · ··· ·
5. En polynukleotidsekvens uppvisande en nukleotidsekvens anordnad sä att den uttrycker en fusionspolypeptid enligt nägot av föregäende patentkrav. • · · ··· • · · • · · «aa a a a «aa a • a a a a · · a a « a < « « a a « a « « a a i i a a a a a a a a a a i a a a « a a
FI915073A 1989-04-29 1991-10-28 Förfarande för framsätllning av fusionsproteiner innehållande N-terminalfragmenter av mänsklig serumalbumin FI104255B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB898909916A GB8909916D0 (en) 1989-04-29 1989-04-29 Polypeptides
GB8909916 1989-04-29
PCT/GB1990/000650 WO1990013653A1 (en) 1989-04-29 1990-04-26 Fusion proteins containing n-terminal fragments of human serum albumin
GB9000650 1990-04-26

Publications (3)

Publication Number Publication Date
FI915073A0 FI915073A0 (fi) 1991-10-28
FI104255B1 FI104255B1 (sv) 1999-12-15
FI104255B true FI104255B (sv) 1999-12-15

Family

ID=10656000

Family Applications (1)

Application Number Title Priority Date Filing Date
FI915073A FI104255B (sv) 1989-04-29 1991-10-28 Förfarande för framsätllning av fusionsproteiner innehållande N-terminalfragmenter av mänsklig serumalbumin

Country Status (12)

Country Link
EP (1) EP0470165A1 (sv)
JP (1) JP2781459B2 (sv)
KR (1) KR100227167B1 (sv)
AU (1) AU630450B2 (sv)
CA (1) CA2015687C (sv)
FI (1) FI104255B (sv)
GB (2) GB8909916D0 (sv)
HU (1) HUT61049A (sv)
IE (1) IE67651B1 (sv)
IL (1) IL94243A (sv)
WO (1) WO1990013653A1 (sv)
ZA (1) ZA903237B (sv)

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US5610148A (en) * 1991-01-18 1997-03-11 University College London Macroscopically oriented cell adhesion protein for wound treatment
US5629287A (en) * 1991-01-18 1997-05-13 University College London Depot formulations
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FR2686900B1 (fr) * 1992-01-31 1995-07-21 Rhone Poulenc Rorer Sa Nouveaux polypeptides ayant une activite de stimulation des colonies de granulocytes, leur preparation et compositions pharmaceutiques les contenant.
WO1994016085A2 (en) * 1992-12-30 1994-07-21 Zymogenetics, Inc. Hybrid proteins having cross-linking and tissue-binding activities
GB9408466D0 (en) * 1994-04-27 1994-06-22 Univ Nottingham Cloning and functional expression of neurotoxins
US5543308A (en) * 1994-10-18 1996-08-06 New England Biolabs, Inc. Isolated DNA encoding the FSEI restriction endonuclease and related methods for producing the same
US5641483A (en) * 1995-06-07 1997-06-24 Beaulieu; Andre Wound healing formulations containing human plasma fibronectin
US7112320B1 (en) 1995-06-07 2006-09-26 Andre Beaulieu Solid wound healing formulations containing fibronectin
US5877149A (en) 1995-06-07 1999-03-02 Beaulieu; Andre Deepithelialized skin diffusion cell system
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US6423512B1 (en) 1996-07-26 2002-07-23 Novartis Ag Fusion polypeptides
AR008077A1 (es) * 1996-07-26 1999-12-09 Talarico Salinas Laura Beatriz Un polipeptido de fusion o una sal del mismo, su uso, un proceso para prepararlos, una composicion farmaceutica que los comprende, y un vector.
US5932693A (en) * 1996-12-10 1999-08-03 Washington University Antithrombotic peptides
WO1999037793A1 (en) 1998-01-23 1999-07-29 Novo Nordisk A/S Process for making desired polypeptides in yeast
JP4087563B2 (ja) 1998-06-15 2008-05-21 ジーティーシー バイオセラピューティックス インコーポレイテッド エリスロポエチン類似体−ヒト血清アルブミン融合物
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP1803730A1 (en) * 2000-04-12 2007-07-04 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
JP4949607B2 (ja) 2001-08-15 2012-06-13 タカラバイオ株式会社 抗原特異的細胞傷害性ｔ細胞拡大培養方法
EP1997829A1 (en) 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Albumin fusion proteins
ES2397319T3 (es) 2002-03-25 2013-03-06 Takara Bio Inc. Proceso para producir linfocitos citotóxicos
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
KR20060039940A (ko) 2003-08-22 2006-05-09 다카라 바이오 가부시키가이샤 세포 상해성 림프구의 제조 방법
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JP5936112B2 (ja) 2009-02-11 2016-06-15 アルブミディクス アクティーゼルスカブ アルブミン変異体及び複合体
BR112012009450A2 (pt) 2009-10-30 2017-05-23 Novozymes Biopharma Dk As variantes de albumina
US10233228B2 (en) 2010-04-09 2019-03-19 Albumedix Ltd Albumin derivatives and variants
KR102103476B1 (ko) 2011-06-24 2020-04-23 더 리젠츠 오브 더 유니버시티 오브 콜로라도, 어 바디 코포레이트 알파-1 안티트립신 융합 분자용 조성물, 방법 및 용도
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CN104736559B (zh) 2012-03-16 2022-04-08 阿尔布梅迪克斯医疗有限公司 白蛋白变体
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GB8725529D0 (en) * 1987-10-30 1987-12-02 Delta Biotechnology Ltd Polypeptides

Also Published As

Publication number Publication date
WO1990013653A1 (en) 1990-11-15
FI915073A0 (fi) 1991-10-28
IE901554L (en) 1990-10-29
FI104255B1 (sv) 1999-12-15
GB2246783A (en) 1992-02-12
AU630450B2 (en) 1992-10-29
IL94243A0 (en) 1991-01-31
JPH04506598A (ja) 1992-11-19
HU904413D0 (en) 1992-01-28
CA2015687A1 (en) 1990-10-29
IL94243A (en) 1995-10-31
KR100227167B1 (ko) 1999-10-15
JP2781459B2 (ja) 1998-07-30
AU5564690A (en) 1990-11-29
GB9119043D0 (en) 1991-12-04
HUT61049A (en) 1992-11-30
ZA903237B (en) 1991-03-27
GB2246783B (en) 1992-10-14
CA2015687C (en) 2000-08-29
IE67651B1 (en) 1996-04-17
KR920701451A (ko) 1992-08-11
GB8909916D0 (en) 1989-06-14
EP0470165A1 (en) 1992-02-12

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