FI104376B - För Hb A1c specifika monoklonala antikroppar - Google Patents
För Hb A1c specifika monoklonala antikroppar Download PDFInfo
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- FI104376B FI104376B FI904226A FI904226A FI104376B FI 104376 B FI104376 B FI 104376B FI 904226 A FI904226 A FI 904226A FI 904226 A FI904226 A FI 904226A FI 104376 B FI104376 B FI 104376B
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- glycosylated
- antibody
- hemoglobin
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Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (10)
1. Monoklonal antikropp eller ett fragment därav, som innehäller antikroppens bindningsställe, k ä n - 5 netecknad av att den binder sig specifikt vid den glykosylerade, N-terminala peptidsekvensen i β-under- enheten av mänskligt hemoglobin.
2. Monoklonal antikropp eller ett fragment därav enligt patentkrav 1, kännetecknad av att den 10 specifikt binder sig vid en glykosylerad peptidrest med formeln Glyko-(NH)Val-His-AA där Glyko-(NH)Vai betecknar en icke-enzymatiskt glykosylerad valinrest och AA är en bindning eller en 15 eller flera ytterligare aminosyrarester.
3. Monoklonal antikropp eller ett fragment därav enligt patentkrav 2, kännetecknad av att AA är en sekvens som innehäller 1-12 aminosyror och som motsvarar β-underenhetens N-ände av mänskligt hemoglobin.
4. Monoklonal antikropp eller ett fragment därav : enligt nägot av patentkraven 1-3, känne- tecknad av att den är bildad mot en immunogen, som • « ;\j innehäller en glykosylerad peptid som är kemiskt förbunden • ( med ett immunogent bärarämne, varvid den glykosylerade s'·' 25 peptiden har minst tvä aminosyraenheter, som motsvarar N- • · !!! änden av hemoglobinets β-underenhet, eller mot ett • i i * * denaturerat eller spjälkat protein.
5. Monoklonal antikropp eller ett fragment därav ’ enligt nägot av patentkraven 1-4, k ä n n e- • · · *.· · 30 tecknad av att den binder sig specifikt tili nämnda ....: glykosylerade, N-terminala peptidsekvens, dä tillräckligt av den frambringats sä, att antikroppens tillträde i den är steriskt möjligt.
' ‘ 6. Monoklonal antikropp eller ett fragment därav 35 enligt patentkrav 5, kännetecknad av att nämnda glykosylerade peptidsekvens har frambringats för 104376 antikroppen medelst fysikalisk eller kemisk denaturering eller spjälkning.
7. Monoklonal antikropp eller ett fragment därav enligt patentkrav 5 eller 6, kännetecknad av 5 att nämnda glykosylerade peptidsekvens har frambringats för antikroppen medelst kemiskt denaturering genom att bringa den i kontakt med ett kaotropiskt ämne, förmcinligt guanidin, urea, tiocyanat eller ett detergent.
8. Monoklonal antikropp eller ett fragment därav 10 enligt nägot av patentkraven 1-4, kännetecknad av att den binder sig specifikt tili nämnda glykosylerade, N-terminala peptidsekvens eller hemoglobin, som är adsorberad tili en fast yta förmänligt framställd av polystyren eller cellulosa.
9. Hybridomcellinje, som utsöndrar monoklonala antikroppar, som binder sig specifikt tili den glykosylerade, N-terminala peptidsekvensen i hemoglobinets β-undergrupp och vars ATCC-deponeringsnummer är HB 8639 eller 8869.
10. Monoklonala antikroppar mot hemoglobin AiC producerade av hybridomer, vilkas ATCC-deponeringsnummer är HB 8639 eller 8869. • ·· · • · · • · • · tn • · · • · « • · · • · · e * I · · • · · • * * « f ( |
Applications Claiming Priority (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66581184A | 1984-10-29 | 1984-10-29 | |
| US66581184 | 1984-10-29 | ||
| US06/763,193 US4647654A (en) | 1984-10-29 | 1985-08-08 | Peptides useful in preparing hemoglobin A1c immunogens |
| US76319385 | 1985-08-08 | ||
| US77973185 | 1985-09-27 | ||
| US06/779,731 US4727036A (en) | 1985-08-08 | 1985-09-27 | Antibodies for use in determining hemoglobin A1c |
| US06/779,730 US4658022A (en) | 1985-08-08 | 1985-09-27 | Binding of antibody reagents to denatured protein analytes |
| US77973085 | 1985-09-27 | ||
| FI854187A FI84107C (sv) | 1984-10-29 | 1985-10-25 | HbA1c:s immunologiska bestämningsförfarande som innefattar denaturerin g |
| FI854187 | 1985-10-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FI904226A0 FI904226A0 (fi) | 1990-08-27 |
| FI104376B true FI104376B (sv) | 2000-01-14 |
Family
ID=27514615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI904226A FI104376B (sv) | 1984-10-29 | 1990-08-27 | För Hb A1c specifika monoklonala antikroppar |
Country Status (1)
| Country | Link |
|---|---|
| FI (1) | FI104376B (sv) |
-
1990
- 1990-08-27 FI FI904226A patent/FI104376B/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI904226A0 (fi) | 1990-08-27 |
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| GB | Transfer or assigment of application |
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| FG | Patent granted |
Owner name: BAYER CORPORATION |
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| MA | Patent expired |